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The map label for this gene is mdh

Identifier: 108762306

GI number: 108762306

Start: 4122581

End: 4123525

Strand: Direct

Name: mdh

Synonym: MXAN_3538

Alternate gene names: 108762306

Gene position: 4122581-4123525 (Clockwise)

Preceding gene: 108759727

Following gene: 108761290

Centisome position: 45.11

GC content: 66.88

Gene sequence:

>945_bases
ATGGCTCAGAATGGCAAGAAGAAGATCGGCCTCATCGGCGGCGGTCAGATCGGCGGCAACCTGGCGCTGCTCGCCGTGCA
GAAGTCGCTCGGTGACGTGGTGCTGTACGACATCCCGGCGGCCGAGGGTCTGGTCAAGGGCAAGGCGCTGGACATCAACC
AGCTGGCCGCGGTCGACGGTTACGACTGCCGCGTGAAGGGCACCACGGACTGGAAGGACGTGGCTGGCTCGGACGTGATC
ATCATCACGGCCGGCATGCCCCGCAAGCCGGGCATGTCCCGCGAGGACCTGCTCGAGATCAACCTGAAGATCATGACGGA
CGTGGCGGGCAACATCAAGCAGCACGCCCCCAACGCCTTCGTCATCAACGTGGCCAACCCGCTGGACGCGATGGTGTTCG
CGCTCCACAAGATCGCCGGTCTGCCCAAGCACATGGTCGCGGGCATGGCGGGCGTGCTGGACACCAGCCGCTTCAAGTGC
TTCGTCGCCGAGGCGCTGGGCTGCTCCATCCGCGACGTGGAGGCCCTGGTGCTGGGCGGCCACGGCGACGACATGGTCCC
GCTCGTCCGCCACAGCACGGTGGGCGGCGTGCCCCTGACGGAGCTGATCGCCAAGGACAAGCTGGACGCCATCATCAAGC
GCACCCGTGAGGGCGGCGCGGAGCTGGTCGGTCTGTACAAGACGGGCAGCGCCTACTTCGGCCCCGCGGCGTCCGCCATC
GCCATGGCGGAGAGCTTCCTGCAGGACCGCAAGCGCGTGCTGCCGGCCGCCGCCCTCCTGGAGGGCCAGTACGGCATCAA
CGGCTACTTCTTCGGCGTCCCCGTGCAGATCGGCGCGGGCGGCGTGGAGAAGATCCACACCGTCGAGCTGAACGATGGTG
AGAAGGCCGAGCTGGAGAAGTCCTTCCAGTCGGTGAAGAAGACGGTCGACTCCGTCAAGCTGTAG

Upstream 100 bases:

>100_bases
AAGGGCAGAGCGGCGTCTCCGAGGTCAAGTGCTCCGAGTTCGGGCAGGCCATCATCAAGAACATGTAGTCCCCGGTCGGG
GATCTGGAAGAGGCGAATCC

Downstream 100 bases:

>100_bases
TCGCTGGACAGTCACGTGCGGCCCGCTGGGGAAACCTGGCGGGCCCTGTGCCTGATGCTGTGGATGACGCGGGCTAATTG
CCCCGGACGCCATCGTTGCA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MAQNGKKKIGLIGGGQIGGNLALLAVQKSLGDVVLYDIPAAEGLVKGKALDINQLAAVDGYDCRVKGTTDWKDVAGSDVI
IITAGMPRKPGMSREDLLEINLKIMTDVAGNIKQHAPNAFVINVANPLDAMVFALHKIAGLPKHMVAGMAGVLDTSRFKC
FVAEALGCSIRDVEALVLGGHGDDMVPLVRHSTVGGVPLTELIAKDKLDAIIKRTREGGAELVGLYKTGSAYFGPAASAI
AMAESFLQDRKRVLPAAALLEGQYGINGYFFGVPVQIGAGGVEKIHTVELNDGEKAELEKSFQSVKKTVDSVKL

Sequences:

>Translated_314_residues
MAQNGKKKIGLIGGGQIGGNLALLAVQKSLGDVVLYDIPAAEGLVKGKALDINQLAAVDGYDCRVKGTTDWKDVAGSDVI
IITAGMPRKPGMSREDLLEINLKIMTDVAGNIKQHAPNAFVINVANPLDAMVFALHKIAGLPKHMVAGMAGVLDTSRFKC
FVAEALGCSIRDVEALVLGGHGDDMVPLVRHSTVGGVPLTELIAKDKLDAIIKRTREGGAELVGLYKTGSAYFGPAASAI
AMAESFLQDRKRVLPAAALLEGQYGINGYFFGVPVQIGAGGVEKIHTVELNDGEKAELEKSFQSVKKTVDSVKL
>Mature_313_residues
AQNGKKKIGLIGGGQIGGNLALLAVQKSLGDVVLYDIPAAEGLVKGKALDINQLAAVDGYDCRVKGTTDWKDVAGSDVII
ITAGMPRKPGMSREDLLEINLKIMTDVAGNIKQHAPNAFVINVANPLDAMVFALHKIAGLPKHMVAGMAGVLDTSRFKCF
VAEALGCSIRDVEALVLGGHGDDMVPLVRHSTVGGVPLTELIAKDKLDAIIKRTREGGAELVGLYKTGSAYFGPAASAIA
MAESFLQDRKRVLPAAALLEGQYGINGYFFGVPVQIGAGGVEKIHTVELNDGEKAELEKSFQSVKKTVDSVKL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=316, Percent_Identity=32.2784810126582, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI221136809, Length=316, Percent_Identity=32.2784810126582, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI260099723, Length=308, Percent_Identity=30.5194805194805, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI5031857, Length=308, Percent_Identity=30.5194805194805, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI291575128, Length=303, Percent_Identity=30.03300330033, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI4557032, Length=303, Percent_Identity=30.03300330033, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI15082234, Length=316, Percent_Identity=27.5316455696203, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI9257228, Length=312, Percent_Identity=28.8461538461538, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4504973, Length=312, Percent_Identity=28.8461538461538, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI260099727, Length=201, Percent_Identity=32.3383084577114, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI21735621, Length=303, Percent_Identity=31.023102310231, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI260099725, Length=201, Percent_Identity=32.3383084577114, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI207028494, Length=186, Percent_Identity=31.1827956989247, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789632, Length=319, Percent_Identity=30.4075235109718, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535107, Length=284, Percent_Identity=25.7042253521127, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17554310, Length=319, Percent_Identity=29.153605015674, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322765, Length=303, Percent_Identity=30.03300330033, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320125, Length=318, Percent_Identity=25.1572327044025, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17136226, Length=304, Percent_Identity=27.3026315789474, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24647881, Length=322, Percent_Identity=30.4347826086957, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI45550422, Length=305, Percent_Identity=27.5409836065574, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24663599, Length=318, Percent_Identity=27.6729559748428, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24663595, Length=288, Percent_Identity=26.3888888888889, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_MYXXA (Q6ZZC6)

Other databases:

- EMBL:   AJ633546
- ProteinModelPortal:   Q6ZZC6
- SMR:   Q6ZZC6
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33044; Mature: 32913

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00867 CPSASE_2

Important sites: ACT_SITE 181-181 BINDING 37-37 BINDING 88-88 BINDING 94-94 BINDING 101-101 BINDING 126-126 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQNGKKKIGLIGGGQIGGNLALLAVQKSLGDVVLYDIPAAEGLVKGKALDINQLAAVDG
CCCCCCCEEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCCHHCCCCCCHHHHHCCCC
YDCRVKGTTDWKDVAGSDVIIITAGMPRKPGMSREDLLEINLKIMTDVAGNIKQHAPNAF
CEEEEECCCCHHHCCCCCEEEEECCCCCCCCCCHHHHHEEEEEEEECHHCCHHHCCCCEE
VINVANPLDAMVFALHKIAGLPKHMVAGMAGVLDTSRFKCFVAEALGCSIRDVEALVLGG
EEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECC
HGDDMVPLVRHSTVGGVPLTELIAKDKLDAIIKRTREGGAELVGLYKTGSAYFGPAASAI
CCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHH
AMAESFLQDRKRVLPAAALLEGQYGINGYFFGVPVQIGAGGVEKIHTVELNDGEKAELEK
HHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEEEEEEECCCCCCEEEEEECCCCCHHHHHH
SFQSVKKTVDSVKL
HHHHHHHHHHHCCC
>Mature Secondary Structure 
AQNGKKKIGLIGGGQIGGNLALLAVQKSLGDVVLYDIPAAEGLVKGKALDINQLAAVDG
CCCCCCEEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCCHHCCCCCCHHHHHCCCC
YDCRVKGTTDWKDVAGSDVIIITAGMPRKPGMSREDLLEINLKIMTDVAGNIKQHAPNAF
CEEEEECCCCHHHCCCCCEEEEECCCCCCCCCCHHHHHEEEEEEEECHHCCHHHCCCCEE
VINVANPLDAMVFALHKIAGLPKHMVAGMAGVLDTSRFKCFVAEALGCSIRDVEALVLGG
EEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECC
HGDDMVPLVRHSTVGGVPLTELIAKDKLDAIIKRTREGGAELVGLYKTGSAYFGPAASAI
CCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHH
AMAESFLQDRKRVLPAAALLEGQYGINGYFFGVPVQIGAGGVEKIHTVELNDGEKAELEK
HHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEEEEEEECCCCCCEEEEEECCCCCHHHHHH
SFQSVKKTVDSVKL
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA