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The map label for this gene is hemK [H]

Identifier: 108759552

GI number: 108759552

Start: 6141434

End: 6142315

Strand: Direct

Name: hemK [H]

Synonym: MXAN_4908

Alternate gene names: 108759552

Gene position: 6141434-6142315 (Clockwise)

Preceding gene: 108759492

Following gene: 108761915

Centisome position: 67.19

GC content: 71.77

Gene sequence:

>882_bases
ATGAGCAGCGAGCCCTGGACCATCCGCAGGGTCCTCACCTGGACGACGCAGCACTTCGAGAAGCGACAGGTGGATGCGCC
GCGGCTCACCGCGGAAATCCTCCTGTCGCACGTGCTCAAGCTCAGCCGCGTCCGGCTGTACGTGGACCTGGACCGCCCGC
TCTCCAAGGACGAGCTGGGCGCCTACCGTGCCCTCATCGAGCGGCGCATGGCGGGCGAGCCCACGCAGTACCTGACGGGG
GTGCGTGAGTTCTACAACCGCCCCTTCAAGGTGGACGCCCGGGTGCTGATTCCGCGCCCGGAGACGGAGCTGTTGGTGGA
GGCGGCGCTGCGCATGCTTCCCAAGGACGCGCCCGGCCGCGCGCTGGACGTGTGCACCGGCTCCGGCTGCATCGCCATCA
GCCTCGCGGCCGAGCGGCCACAGGCCACCGTCATCGCCACCGACCTGTCTCCGGACGCCTGCGCGCTGGCGCGGGAGAAC
GCCCAGGCGCTGGGCGTGGCGGACCGGGTGACGGTGCTGCAGGGCGACCTCTTCACCCCGGTGCCCGCGGGCGAGCGCTT
CCAGGTGGTGGTCTCCAACCCGCCGTACATCGCCTCCGGGGAGATTCCCGGCCTGTCCGCCGAGGTGCGACGCGAGCCGA
CGCTGGCGCTCGACGGCGGGCCGGACGGGCTGGTGGCGGTGCGCCGAGTGGTGACGGGTGCGCGCCAGTGGTTGGAGCCT
GGCGGCCTCCTTGCATTGGAGATTGGCGAGGACCAGGGCCCCGCCGTCCTGGAGCTCCTGCGTGCCGCGGGTTACGCGGA
CGCGCGGGTGGAGAAGGACCTGGAGCGGCGGGAACGGATGGCATTTGGGACACAGCCCGTGGCCAGCGAGCCACAGGGCT
GA

Upstream 100 bases:

>100_bases
TCATGGCCGGTGACGTGGAAGACGTCATCACCGCCTGCCGGACCTTCTACCAGGCCGAAGCGCTCAAGGCGCAGACGGCC
GGCGGCCCGAAGCCCTCCGC

Downstream 100 bases:

>100_bases
AGACGCAGGGAACCATGGACAAGATCGTCATCAAGGGCGGCCAGGCGCTGCACGGCGAAGTGCAGGCCTCGGGCGCGAAG
AACGCGGCGCTGCCCATCCT

Product: putative protein methyltransferase HemK

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSSEPWTIRRVLTWTTQHFEKRQVDAPRLTAEILLSHVLKLSRVRLYVDLDRPLSKDELGAYRALIERRMAGEPTQYLTG
VREFYNRPFKVDARVLIPRPETELLVEAALRMLPKDAPGRALDVCTGSGCIAISLAAERPQATVIATDLSPDACALAREN
AQALGVADRVTVLQGDLFTPVPAGERFQVVVSNPPYIASGEIPGLSAEVRREPTLALDGGPDGLVAVRRVVTGARQWLEP
GGLLALEIGEDQGPAVLELLRAAGYADARVEKDLERRERMAFGTQPVASEPQG

Sequences:

>Translated_293_residues
MSSEPWTIRRVLTWTTQHFEKRQVDAPRLTAEILLSHVLKLSRVRLYVDLDRPLSKDELGAYRALIERRMAGEPTQYLTG
VREFYNRPFKVDARVLIPRPETELLVEAALRMLPKDAPGRALDVCTGSGCIAISLAAERPQATVIATDLSPDACALAREN
AQALGVADRVTVLQGDLFTPVPAGERFQVVVSNPPYIASGEIPGLSAEVRREPTLALDGGPDGLVAVRRVVTGARQWLEP
GGLLALEIGEDQGPAVLELLRAAGYADARVEKDLERRERMAFGTQPVASEPQG
>Mature_292_residues
SSEPWTIRRVLTWTTQHFEKRQVDAPRLTAEILLSHVLKLSRVRLYVDLDRPLSKDELGAYRALIERRMAGEPTQYLTGV
REFYNRPFKVDARVLIPRPETELLVEAALRMLPKDAPGRALDVCTGSGCIAISLAAERPQATVIATDLSPDACALARENA
QALGVADRVTVLQGDLFTPVPAGERFQVVVSNPPYIASGEIPGLSAEVRREPTLALDGGPDGLVAVRRVVTGARQWLEPG
GLLALEIGEDQGPAVLELLRAAGYADARVEKDLERRERMAFGTQPVASEPQG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=256, Percent_Identity=34.765625, Blast_Score=111, Evalue=9e-25,
Organism=Escherichia coli, GI1787463, Length=259, Percent_Identity=41.6988416988417, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI87082085, Length=262, Percent_Identity=33.587786259542, Blast_Score=130, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24582226, Length=260, Percent_Identity=31.9230769230769, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32019; Mature: 31888

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEPWTIRRVLTWTTQHFEKRQVDAPRLTAEILLSHVLKLSRVRLYVDLDRPLSKDELG
CCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHH
AYRALIERRMAGEPTQYLTGVREFYNRPFKVDARVLIPRPETELLVEAALRMLPKDAPGR
HHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCEEEECCCCHHHHHHHHHHHCCCCCCCC
ALDVCTGSGCIAISLAAERPQATVIATDLSPDACALARENAQALGVADRVTVLQGDLFTP
EEEEECCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHCCHHHHCHHHHEEEEECCCCCC
VPAGERFQVVVSNPPYIASGEIPGLSAEVRREPTLALDGGPDGLVAVRRVVTGARQWLEP
CCCCCEEEEEECCCCEEECCCCCCCCHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCC
GGLLALEIGEDQGPAVLELLRAAGYADARVEKDLERRERMAFGTQPVASEPQG
CCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SSEPWTIRRVLTWTTQHFEKRQVDAPRLTAEILLSHVLKLSRVRLYVDLDRPLSKDELG
CCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHH
AYRALIERRMAGEPTQYLTGVREFYNRPFKVDARVLIPRPETELLVEAALRMLPKDAPGR
HHHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCEEEECCCCHHHHHHHHHHHCCCCCCCC
ALDVCTGSGCIAISLAAERPQATVIATDLSPDACALARENAQALGVADRVTVLQGDLFTP
EEEEECCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHCCHHHHCHHHHEEEEECCCCCC
VPAGERFQVVVSNPPYIASGEIPGLSAEVRREPTLALDGGPDGLVAVRRVVTGARQWLEP
CCCCCEEEEEECCCCEEECCCCCCCCHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHCCC
GGLLALEIGEDQGPAVLELLRAAGYADARVEKDLERRERMAFGTQPVASEPQG
CCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA