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The map label for this gene is prfA

Identifier: 108759492

GI number: 108759492

Start: 6140349

End: 6141437

Strand: Direct

Name: prfA

Synonym: MXAN_4907

Alternate gene names: 108759492

Gene position: 6140349-6141437 (Clockwise)

Preceding gene: 108760803

Following gene: 108759552

Centisome position: 67.18

GC content: 67.31

Gene sequence:

>1089_bases
ATGATTGACAAACTCGAAGACGTCGAACGCCGGTTCGAACGCCTGACCGCCGACCTGTCGAACCCCGACGTGCTCGCCGA
CTCGGCGAGGCTCCAGAAGGTGTCCAAGGAGCGCGCGGGCCTGGAGAAGCTGGTCGAGGCCTTCAGGACCTACCGCAAGG
TGCTCGCCGACCTCAGCGAGGTCGAAGCATGGCTCGGCAGCAGCGACGCCGACGAGAAGGCCTTCGCACGCGAGTCCCTG
CCCGGACTGAAGGAACAGCGCGACGAGCTGGAGGCCAGCCTCAAGATTCTCCTCCTGCCCAAAGACCCGAATGACGAGAA
GAACGTCATCCTGGAAATCCGCGCGGGCGCGGGCGGTGACGAGGCCGCCCTCTTCGCCGAGGAGGTCATGCAGATGTACC
TCCGCTACGCGGACCGCCGGGGCTGGAAGGCGGACATCCTGGACATGAGCCCGGGCAACGCCGGCGGCGTGAAGGACGCC
ACGGTGACGCTGTCCGGTGACGCCGTGTTCAGCAGCATGAAGTACGAGTCCGGCGTGCACCGCGTGCAGCGCGTGCCGGC
CACGGAGACGCAGGGCCGCATCCACACGTCCACCATCACGGTGTCGGTGATGCCGGAGGCGGAGGACGTGGACGTGCAGG
TGAACCCGGCGGACATCGAGATGCAGGTGATGCGCTCCACGGGCTCGGGCGGCCAGAGCGTCAACACCACGGACTCCGCG
GTGCGCCTCATCCACCACCCGACGGGCATCGTAGTGAAGTGCCAGCAGGAGAAGAGCCAGTTGAAGAACCGCACCATGGC
CATGCGCATGCTGCGGGCGAAGCTCTACGACATCGAGCAGGAGCGCATCCGCAACGAGCGCGACTCCGCGCGCCGCGCCC
AGGTGGGCACCGGCGACCGCAGCGAGAAGATTCGCACCTACAACTTCCCGCAGGACCGGCTGACGGACCACCGCATCGGC
CTCACGGTGCACAACCTGCCGGGCGTCATGGCCGGTGACGTGGAAGACGTCATCACCGCCTGCCGGACCTTCTACCAGGC
CGAAGCGCTCAAGGCGCAGACGGCCGGCGGCCCGAAGCCCTCCGCATGA

Upstream 100 bases:

>100_bases
GCCCTTTTTTCATGCTCCCGCCCAAGCGTCCAAGGAATTTCTTGACTCCGCCCGCCCTTGCCAGAGGCCGTGCAAAGGGG
CAATACCCCCCGGAACGACG

Downstream 100 bases:

>100_bases
GCAGCGAGCCCTGGACCATCCGCAGGGTCCTCACCTGGACGACGCAGCACTTCGAGAAGCGACAGGTGGATGCGCCGCGG
CTCACCGCGGAAATCCTCCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MIDKLEDVERRFERLTADLSNPDVLADSARLQKVSKERAGLEKLVEAFRTYRKVLADLSEVEAWLGSSDADEKAFARESL
PGLKEQRDELEASLKILLLPKDPNDEKNVILEIRAGAGGDEAALFAEEVMQMYLRYADRRGWKADILDMSPGNAGGVKDA
TVTLSGDAVFSSMKYESGVHRVQRVPATETQGRIHTSTITVSVMPEAEDVDVQVNPADIEMQVMRSTGSGGQSVNTTDSA
VRLIHHPTGIVVKCQQEKSQLKNRTMAMRMLRAKLYDIEQERIRNERDSARRAQVGTGDRSEKIRTYNFPQDRLTDHRIG
LTVHNLPGVMAGDVEDVITACRTFYQAEALKAQTAGGPKPSA

Sequences:

>Translated_362_residues
MIDKLEDVERRFERLTADLSNPDVLADSARLQKVSKERAGLEKLVEAFRTYRKVLADLSEVEAWLGSSDADEKAFARESL
PGLKEQRDELEASLKILLLPKDPNDEKNVILEIRAGAGGDEAALFAEEVMQMYLRYADRRGWKADILDMSPGNAGGVKDA
TVTLSGDAVFSSMKYESGVHRVQRVPATETQGRIHTSTITVSVMPEAEDVDVQVNPADIEMQVMRSTGSGGQSVNTTDSA
VRLIHHPTGIVVKCQQEKSQLKNRTMAMRMLRAKLYDIEQERIRNERDSARRAQVGTGDRSEKIRTYNFPQDRLTDHRIG
LTVHNLPGVMAGDVEDVITACRTFYQAEALKAQTAGGPKPSA
>Mature_362_residues
MIDKLEDVERRFERLTADLSNPDVLADSARLQKVSKERAGLEKLVEAFRTYRKVLADLSEVEAWLGSSDADEKAFARESL
PGLKEQRDELEASLKILLLPKDPNDEKNVILEIRAGAGGDEAALFAEEVMQMYLRYADRRGWKADILDMSPGNAGGVKDA
TVTLSGDAVFSSMKYESGVHRVQRVPATETQGRIHTSTITVSVMPEAEDVDVQVNPADIEMQVMRSTGSGGQSVNTTDSA
VRLIHHPTGIVVKCQQEKSQLKNRTMAMRMLRAKLYDIEQERIRNERDSARRAQVGTGDRSEKIRTYNFPQDRLTDHRIG
LTVHNLPGVMAGDVEDVITACRTFYQAEALKAQTAGGPKPSA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=296, Percent_Identity=44.2567567567568, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI34577120, Length=341, Percent_Identity=37.8299120234604, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI166795305, Length=192, Percent_Identity=41.1458333333333, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1787462, Length=353, Percent_Identity=46.1756373937677, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=36.2275449101796, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=240, Percent_Identity=41.25, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=326, Percent_Identity=40.4907975460123, Blast_Score=236, Evalue=6e-63,
Organism=Drosophila melanogaster, GI19921226, Length=243, Percent_Identity=46.5020576131687, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_MYXXA (Q93NC7)

Other databases:

- EMBL:   AF377339
- ProteinModelPortal:   Q93NC7
- SMR:   Q93NC7
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40143; Mature: 40143

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKLEDVERRFERLTADLSNPDVLADSARLQKVSKERAGLEKLVEAFRTYRKVLADLSE
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEAWLGSSDADEKAFARESLPGLKEQRDELEASLKILLLPKDPNDEKNVILEIRAGAGGD
HHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEECCCCCC
EAALFAEEVMQMYLRYADRRGWKADILDMSPGNAGGVKDATVTLSGDAVFSSMKYESGVH
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
RVQRVPATETQGRIHTSTITVSVMPEAEDVDVQVNPADIEMQVMRSTGSGGQSVNTTDSA
HHHHCCCCCCCCEEEEEEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCHHHH
VRLIHHPTGIVVKCQQEKSQLKNRTMAMRMLRAKLYDIEQERIRNERDSARRAQVGTGDR
HHHEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCC
SEKIRTYNFPQDRLTDHRIGLTVHNLPGVMAGDVEDVITACRTFYQAEALKAQTAGGPKP
CCCCEECCCCHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
SA
CC
>Mature Secondary Structure
MIDKLEDVERRFERLTADLSNPDVLADSARLQKVSKERAGLEKLVEAFRTYRKVLADLSE
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEAWLGSSDADEKAFARESLPGLKEQRDELEASLKILLLPKDPNDEKNVILEIRAGAGGD
HHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEECCCCCC
EAALFAEEVMQMYLRYADRRGWKADILDMSPGNAGGVKDATVTLSGDAVFSSMKYESGVH
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
RVQRVPATETQGRIHTSTITVSVMPEAEDVDVQVNPADIEMQVMRSTGSGGQSVNTTDSA
HHHHCCCCCCCCEEEEEEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCHHHH
VRLIHHPTGIVVKCQQEKSQLKNRTMAMRMLRAKLYDIEQERIRNERDSARRAQVGTGDR
HHHEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCCC
SEKIRTYNFPQDRLTDHRIGLTVHNLPGVMAGDVEDVITACRTFYQAEALKAQTAGGPKP
CCCCEECCCCHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
SA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA