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The map label for this gene is rumA [H]

Identifier: 108758357

GI number: 108758357

Start: 4552657

End: 4553952

Strand: Reverse

Name: rumA [H]

Synonym: MXAN_3787

Alternate gene names: 108758357

Gene position: 4553952-4552657 (Counterclockwise)

Preceding gene: 108758933

Following gene: 108756945

Centisome position: 49.83

GC content: 71.76

Gene sequence:

>1296_bases
ATGCTGCCGCTACCCGAACTTCCCATTGAATTGACCATCGAACGCCTCGGCCAGCTCGGCGAAGGCGTGGCCTCCTGGCA
GGGGCGCACCGTCTTCATCCCGGGGACCTTCCCGGGCGACACCGTGCGCGTCCACCTGGAGACGCAAGGCCGTGTGCTGC
GCGGCATCCTGCGACAGGTGGTGTCGCCAGGCCCGGACCGGCGTGACGCGCCCTGCGTGTACGCGTCCGACTGCGGCGGC
TGTGACTGGCTGGCGCTGGCCGAGCCCGCACAGCGCAGCGCGAAGCAGGAGATCGTCCTCTCCACCCTGGAGCACCTGGG
GCACCTGGCTCGGGACAGCTTCACCGTGCGTCCGCTGCTGGTGGCCCCACGCGACTGGGGCTACCGGCGCCGCGCGGTGC
TGCATCCCGCGGGCAAGGGCGCGCTGGGGTACTTCGGCCGGCGCAGCCACGAACGCGTACCCGTGGAAGTCTGCGGCGCG
CTCACGCCCGTGCTCACGGCGCTCCCCGGCAAGCTGGCCCCGCTGCTCAAGCCCCTGGCGAAGGATGCCGAGGAAGTGCT
GCTGCTGGCGGAAGGGGACAAGGCCGCGTTCGCGGTGAATCTCAGCGGCCCGGTGACGGCGCGCCACCTGGAGGCCGCCG
AGGCGGCCGTGCGCGCCCTGCGGCTGGAGGGCGCGGTGCTGACGCCGAAGGAAGGATCGGCTCGGCTTCTTGGCAAGCCG
GCGCTGCGCTCCTACTCGCCGCTGCGGCCAGAAGTCCCCCTGTACCTGCGGCCGGATGCCTTCGCTCAGGCCCATGCCGA
GGCCAACGTGGGGCTGGTGACCTCGGCCGTGTACGAGCTGGGAGCCCGCGAGTCCGACACCGTGCTGGAGCTGTATTCGG
GCAACGGCAACTTCACCTTCCCGCTGGCCGGAACCGCCGCGTCCGTGCTGGGCGTGGAGTCCTCCCCCGTGGGCGTCGAG
TTGGCACAGCGGAGCGCTCACGAGGGTGGGGTGACGAATGTGCGCTTCATCCAGGGCGACGCCCGCAAGGTGTGCGACGG
GCTGGTGGCCGAGCAGCGCCGCTTCGATGTGTGCCTCGCGGACCCGCCGCGCGCGGGAGCCCCGGGCCTGGCGAAGTGGA
TGACCGCCTTGGGGGTGCGCCGCGTGGTGTACGTGGCGTGTGATCCGGCATCCCTGGCTCGGGACGCCGCCGGCCTGGTT
GCCGCGGGCTACAAGCCCCTGGCCCTCCAGGTGGTCGACATGTTCCCTCAGACGCATCACGTCGAGGCCGTGATGTCCTT
CGAGCGGCCGGCCTGA

Upstream 100 bases:

>100_bases
ATGGCGGTGGAGTTCCTGGACGTTGATGAAGACAAGCGAACGCGTATCGCGGAGTTCGTGGAGCGCCTGCGCTCGGAGCT
CCCGCCCGAATAGCGCCTGA

Downstream 100 bases:

>100_bases
ACAGCACCAACACGTACTGTCAGTATTTGCTCTGTTGAGCAGCGAGATTCGGGCGTCCATCGGCGCTCAGGCTACGGCTT
GCCCGATTCCCCTCTGAAAC

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MLPLPELPIELTIERLGQLGEGVASWQGRTVFIPGTFPGDTVRVHLETQGRVLRGILRQVVSPGPDRRDAPCVYASDCGG
CDWLALAEPAQRSAKQEIVLSTLEHLGHLARDSFTVRPLLVAPRDWGYRRRAVLHPAGKGALGYFGRRSHERVPVEVCGA
LTPVLTALPGKLAPLLKPLAKDAEEVLLLAEGDKAAFAVNLSGPVTARHLEAAEAAVRALRLEGAVLTPKEGSARLLGKP
ALRSYSPLRPEVPLYLRPDAFAQAHAEANVGLVTSAVYELGARESDTVLELYSGNGNFTFPLAGTAASVLGVESSPVGVE
LAQRSAHEGGVTNVRFIQGDARKVCDGLVAEQRRFDVCLADPPRAGAPGLAKWMTALGVRRVVYVACDPASLARDAAGLV
AAGYKPLALQVVDMFPQTHHVEAVMSFERPA

Sequences:

>Translated_431_residues
MLPLPELPIELTIERLGQLGEGVASWQGRTVFIPGTFPGDTVRVHLETQGRVLRGILRQVVSPGPDRRDAPCVYASDCGG
CDWLALAEPAQRSAKQEIVLSTLEHLGHLARDSFTVRPLLVAPRDWGYRRRAVLHPAGKGALGYFGRRSHERVPVEVCGA
LTPVLTALPGKLAPLLKPLAKDAEEVLLLAEGDKAAFAVNLSGPVTARHLEAAEAAVRALRLEGAVLTPKEGSARLLGKP
ALRSYSPLRPEVPLYLRPDAFAQAHAEANVGLVTSAVYELGARESDTVLELYSGNGNFTFPLAGTAASVLGVESSPVGVE
LAQRSAHEGGVTNVRFIQGDARKVCDGLVAEQRRFDVCLADPPRAGAPGLAKWMTALGVRRVVYVACDPASLARDAAGLV
AAGYKPLALQVVDMFPQTHHVEAVMSFERPA
>Mature_431_residues
MLPLPELPIELTIERLGQLGEGVASWQGRTVFIPGTFPGDTVRVHLETQGRVLRGILRQVVSPGPDRRDAPCVYASDCGG
CDWLALAEPAQRSAKQEIVLSTLEHLGHLARDSFTVRPLLVAPRDWGYRRRAVLHPAGKGALGYFGRRSHERVPVEVCGA
LTPVLTALPGKLAPLLKPLAKDAEEVLLLAEGDKAAFAVNLSGPVTARHLEAAEAAVRALRLEGAVLTPKEGSARLLGKP
ALRSYSPLRPEVPLYLRPDAFAQAHAEANVGLVTSAVYELGARESDTVLELYSGNGNFTFPLAGTAASVLGVESSPVGVE
LAQRSAHEGGVTNVRFIQGDARKVCDGLVAEQRRFDVCLADPPRAGAPGLAKWMTALGVRRVVYVACDPASLARDAAGLV
AAGYKPLALQVVDMFPQTHHVEAVMSFERPA

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=185, Percent_Identity=30.8108108108108, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI51173878, Length=185, Percent_Identity=30.8108108108108, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1789148, Length=429, Percent_Identity=31.2354312354312, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1790403, Length=176, Percent_Identity=28.9772727272727, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787083, Length=175, Percent_Identity=26.2857142857143, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24666579, Length=193, Percent_Identity=31.0880829015544, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 45950; Mature: 45950

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLPELPIELTIERLGQLGEGVASWQGRTVFIPGTFPGDTVRVHLETQGRVLRGILRQV
CCCCCCCCHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
VSPGPDRRDAPCVYASDCGGCDWLALAEPAQRSAKQEIVLSTLEHLGHLARDSFTVRPLL
HCCCCCCCCCCEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
VAPRDWGYRRRAVLHPAGKGALGYFGRRSHERVPVEVCGALTPVLTALPGKLAPLLKPLA
ECCCCCCCCCEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
KDAEEVLLLAEGDKAAFAVNLSGPVTARHLEAAEAAVRALRLEGAVLTPKEGSARLLGKP
CCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCEECCCC
ALRSYSPLRPEVPLYLRPDAFAQAHAEANVGLVTSAVYELGARESDTVLELYSGNGNFTF
CCCCCCCCCCCCCEEECCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEE
PLAGTAASVLGVESSPVGVELAQRSAHEGGVTNVRFIQGDARKVCDGLVAEQRRFDVCLA
EECCCHHHHEECCCCCCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEC
DPPRAGAPGLAKWMTALGVRRVVYVACDPASLARDAAGLVAAGYKPLALQVVDMFPQTHH
CCCCCCCCHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHH
VEAVMSFERPA
HHHHHHCCCCC
>Mature Secondary Structure
MLPLPELPIELTIERLGQLGEGVASWQGRTVFIPGTFPGDTVRVHLETQGRVLRGILRQV
CCCCCCCCHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
VSPGPDRRDAPCVYASDCGGCDWLALAEPAQRSAKQEIVLSTLEHLGHLARDSFTVRPLL
HCCCCCCCCCCEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
VAPRDWGYRRRAVLHPAGKGALGYFGRRSHERVPVEVCGALTPVLTALPGKLAPLLKPLA
ECCCCCCCCCEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
KDAEEVLLLAEGDKAAFAVNLSGPVTARHLEAAEAAVRALRLEGAVLTPKEGSARLLGKP
CCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCCEECCCC
ALRSYSPLRPEVPLYLRPDAFAQAHAEANVGLVTSAVYELGARESDTVLELYSGNGNFTF
CCCCCCCCCCCCCEEECCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEE
PLAGTAASVLGVESSPVGVELAQRSAHEGGVTNVRFIQGDARKVCDGLVAEQRRFDVCLA
EECCCHHHHEECCCCCCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEC
DPPRAGAPGLAKWMTALGVRRVVYVACDPASLARDAAGLVAAGYKPLALQVVDMFPQTHH
CCCCCCCCHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHH
VEAVMSFERPA
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA