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The map label for this gene is istB [H]

Identifier: 108758112

GI number: 108758112

Start: 4216751

End: 4217512

Strand: Direct

Name: istB [H]

Synonym: MXAN_3614

Alternate gene names: 108758112

Gene position: 4216751-4217512 (Clockwise)

Preceding gene: 108757122

Following gene: 108756951

Centisome position: 46.14

GC content: 64.7

Gene sequence:

>762_bases
ATGAGCCCCTTGAAGGAACGCCTCAACACCCTGGGCCTGCATCACACCAGTGCCGCGCTCGATGACCTCATTGCCCTGGC
CACCAAACGCAGGTGGGGCCCCACCGAGTTGCTGGAAGCCATCACCGAGGAAGAGACGAAGGACAGAGCCCGGCGCAGCG
TGGAGCGGCGCCTGTCGCGCAGCAAGCTGGGCGCGCTCAGGACGATGGCCGACTTCGACTGGGGCTGGCCCAAGCGCATC
GACAGGACAGCCGTCGAGGGCGCCCTGGCGCTGGACTTCATGGCCCGCCCCGCCAACGTCGTCCTCGTCGCGCCCCAAGG
ACTGGGCAAGACGATGATTGCTCAGAATATCGCCTACAACGCCGTGCAGGCTGGGCACCCCGTCCTCTTCACCACCGCCT
CCCAGTTGCTGCTCGACTTGGGAAGCCAGGACTCCGCTCGCGCTCTCGACAGGCGCCTGAAGCACTACTGCACTCAGGCG
GGGTTGCTCGTCATCGACGAGATTGGCTACCTCTCCTACGACGCCAGGAACGCCGACCTCCTCTTCCAGGTGGTGTCGCG
CAGGTATGAGAAGAAGCCGATTGTCCTGACGACCAACCTCCCATTCTCCGAGTGGACCACCATCTTCCCCAACGCCGCGT
GCGCCATCGCCCTCATTGACCGCGTCATCCACCACGCGAGCATCATCAGCATCGAGGGCGACAGCTACCGCAGGCGTATG
GCCGAGGAATCACGCGCGGAGCGCAAGGCAAGGAAGAGCTGA

Upstream 100 bases:

>100_bases
TCGTGCTGCCGGATGACCCGCGCGTGCGCGAGCAACGCGTCCCCCAGCACTCCCTCAACCCCTACGACGCCCTCCTCGGG
CGCGACACGGAGCAAGTGCC

Downstream 100 bases:

>100_bases
GCGTCCCCGCGACTCACCGATGCCCACCAGCCTCGCTCTGCGCCCCGCAATCGGAGCGCAGCGCGGGGCCGTCTTGCGTT
GCCTCGACTCGATACCTGCG

Product: IS21 family transposition helper protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSPLKERLNTLGLHHTSAALDDLIALATKRRWGPTELLEAITEEETKDRARRSVERRLSRSKLGALRTMADFDWGWPKRI
DRTAVEGALALDFMARPANVVLVAPQGLGKTMIAQNIAYNAVQAGHPVLFTTASQLLLDLGSQDSARALDRRLKHYCTQA
GLLVIDEIGYLSYDARNADLLFQVVSRRYEKKPIVLTTNLPFSEWTTIFPNAACAIALIDRVIHHASIISIEGDSYRRRM
AEESRAERKARKS

Sequences:

>Translated_253_residues
MSPLKERLNTLGLHHTSAALDDLIALATKRRWGPTELLEAITEEETKDRARRSVERRLSRSKLGALRTMADFDWGWPKRI
DRTAVEGALALDFMARPANVVLVAPQGLGKTMIAQNIAYNAVQAGHPVLFTTASQLLLDLGSQDSARALDRRLKHYCTQA
GLLVIDEIGYLSYDARNADLLFQVVSRRYEKKPIVLTTNLPFSEWTTIFPNAACAIALIDRVIHHASIISIEGDSYRRRM
AEESRAERKARKS
>Mature_252_residues
SPLKERLNTLGLHHTSAALDDLIALATKRRWGPTELLEAITEEETKDRARRSVERRLSRSKLGALRTMADFDWGWPKRID
RTAVEGALALDFMARPANVVLVAPQGLGKTMIAQNIAYNAVQAGHPVLFTTASQLLLDLGSQDSARALDRRLKHYCTQAG
LLVIDEIGYLSYDARNADLLFQVVSRRYEKKPIVLTTNLPFSEWTTIFPNAACAIALIDRVIHHASIISIEGDSYRRRMA
EESRAERKARKS

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 28379; Mature: 28248

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLKERLNTLGLHHTSAALDDLIALATKRRWGPTELLEAITEEETKDRARRSVERRLSR
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKLGALRTMADFDWGWPKRIDRTAVEGALALDFMARPANVVLVAPQGLGKTMIAQNIAYN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHH
AVQAGHPVLFTTASQLLLDLGSQDSARALDRRLKHYCTQAGLLVIDEIGYLSYDARNADL
HHHCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCEECCCCHHH
LFQVVSRRYEKKPIVLTTNLPFSEWTTIFPNAACAIALIDRVIHHASIISIEGDSYRRRM
HHHHHHHHHCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCHHHHHHH
AEESRAERKARKS
HHHHHHHHHHCCC
>Mature Secondary Structure 
SPLKERLNTLGLHHTSAALDDLIALATKRRWGPTELLEAITEEETKDRARRSVERRLSR
CHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKLGALRTMADFDWGWPKRIDRTAVEGALALDFMARPANVVLVAPQGLGKTMIAQNIAYN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHH
AVQAGHPVLFTTASQLLLDLGSQDSARALDRRLKHYCTQAGLLVIDEIGYLSYDARNADL
HHHCCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCEECCCCHHH
LFQVVSRRYEKKPIVLTTNLPFSEWTTIFPNAACAIALIDRVIHHASIISIEGDSYRRRM
HHHHHHHHHCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCHHHHHHH
AEESRAERKARKS
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]