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The map label for this gene is leuC [H]

Identifier: 108757641

GI number: 108757641

Start: 1496990

End: 1498960

Strand: Reverse

Name: leuC [H]

Synonym: MXAN_1282

Alternate gene names: 108757641

Gene position: 1498960-1496990 (Counterclockwise)

Preceding gene: 108760083

Following gene: 108760047

Centisome position: 16.4

GC content: 68.54

Gene sequence:

>1971_bases
ATGAACACCCGACCCATGACCCTGACGCAGAAAATCCTGGCCCACCATGCCCGGCGCCTCGATCGCCCCTGGGTCCAGGC
GGGAGACGTCCTGCAGCTCCGGGTGGACTGGACGATGGCGAGCGAGCTGGCCTGGAACGGCATGGACCGCACCTACCAGG
CCCTGGGACGCCCGGAGCTCCACGACCGGGAGCGCGTCTTCCTCGCCGTGGACCACACGGTGGACGCAGTGACCCTTGCC
AAGGACGTGCGCGTCCAGCGGCTGGTACAGCGCTCGCGTGACTTCGCCCGCGAACGGAGGCTGCGCCACTTCTACGATGC
CAACGAGACCATCCTTCACACGAAGTTCTATCGAGAGCTGGTCCAGCCAGGACAGTTGGTGCTGGGAGCCGATTCACACA
CCACCTCCCATGGAGGGATGGGCGCCTTCGCCATCGGACTGGGTGGAGCGGACATCGTTGCCGCGATGGTACTCGGGGAG
ACATGGCTCGAGGTTCCCGAGGCCATCACCGTGGAGTACCAGGGCACGCCTGCCTTTGGCATCGGCGGCAAGGACATCAT
CCTGAAGACCTTGGGGCTCCTTGGAAGGAACACCGCCGCGCTGGAGCGGACGGTGGAGTACCGCGGAGAAGCCGCGCGCG
GCTTCACCACGGACATGCGCTTCACCATCGCGAACATGACGGCGGAGCTCGGCGGGCTGAATGGCATCTTCGAGCCCGAC
GCGAGGGTCACCGAGTGGCTCGCCATGCGCCCGTCGAACAAGGACGAGGCCCTGTACTTCCGCGCCGATGAGGATGCGCC
CTACGTACAGCGCTTCAGCATCGCGCTGGAGAAGCTCGGGCCGCAGCTCGCCCGGCCCTACGCTCCGGACAACGTGCTGG
AGGTCCAGGACGCGGTAGGGATGGCGGTGGACGGCTGCTTCATCGGTGCGTGCACCACCACCGAGGAGGAGCTCGTGCTG
GCGGCGCTCCTGTTGGAGCAGGCGCTCCGAGGCAAGGCGGCGCGCCCCGCCTCACCCAAGCGGCTCGTGGTGCCCGGGGA
CCTCGCCATCCACGAACGAATGCGGCAAGCCGGCCTGTGGCGGCACTACGAGCAGGCGGGCTTCCGAATCGGCCCGCCAG
GCTGCTCCATGTGTCTGGGCGTGGCCTCAGAAAAGGCCCTTCCCGGCGAGGTGTGGCTCACATCACAGAACCGCAACTAC
GAGAACCGGATGGGGCCCGGATCACACGCTTGGCTCGCCTCGGCGGCCACTGTAGCGGCCTCCTCCCTGGAGATGCGCGT
GGAGGACCCCCGCGCCGCGCTGAAGAGGATCGACCCGTCCGTCCTCGAGCGAATCCTCGCCCGCAAGCCAGCGGGCCGCC
CCATCGAGTTCCCGTCCTCCGAGCCACGACTGCCCATGACGCCCCAGCCACATGACGGCGGCACGCGCGCGGGCACGGCC
TCCGGAGGACGCATCGCTGGACGCATTCAGCGCTTCGGGAACCATGTCGACACGGACGCCATCATCCCGGGCGAGTTCTG
CCACCTTGATGAGCCCTCGGAGCTGGCTCGCCATGCATTCAGGTATGTCCGCCCCGAGTTTCTGGAACGCGTGCAGCAGG
GGCGGACGCTCATCGTCGCAGGGGAGGGCTGGGGCACGGGCAGCTCCCGAGAGCAGGCCGTCTGGGCGCTGGCCGGGGCC
GGCATCAAGGCCGTCATCGCCAGCAGCTATGCCTTCATCCACCGGCGCAACCTGGTGAACGAGGCGCTGCCCCACCTCGT
CGTGACGAGCCCTGCGTTCCACGCCCTGGTCCAGGAAGACGAGGAGCTCGTCGTCGACCTGGAGACGGGCCTGGTGGAGC
ATGTGGCGAGCGGCCAACGGTTCCAGGCCGAGCGGCCCAGCCCCATCGCCCGCGCGCTCATGCAAGAGGGCGGACTCGTT
CCCGCCATCCGGCGCCTCGGCGCCCGGGCGCTCCACCTCGGAGCCCCCTGA

Upstream 100 bases:

>100_bases
GACCCGCACGTAGGGGCGGATTGCCCTGCCGCGTGAGGCTGGTGAGCGGAACGCCATCCTCTTGAGGGGACGCCAGCGCG
TCCCAAGAAAGGCGCTGCAC

Downstream 100 bases:

>100_bases
CCCGGCGCCGTCGTCCTCGGCGGGGCGCGCCGGGCAAGACCGCGCCTGGAAACAGCGCCCGAAACCGCTCGTTGTCAGGG
AACGACCACTCAGGCCGAAG

Product: putative 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MNTRPMTLTQKILAHHARRLDRPWVQAGDVLQLRVDWTMASELAWNGMDRTYQALGRPELHDRERVFLAVDHTVDAVTLA
KDVRVQRLVQRSRDFARERRLRHFYDANETILHTKFYRELVQPGQLVLGADSHTTSHGGMGAFAIGLGGADIVAAMVLGE
TWLEVPEAITVEYQGTPAFGIGGKDIILKTLGLLGRNTAALERTVEYRGEAARGFTTDMRFTIANMTAELGGLNGIFEPD
ARVTEWLAMRPSNKDEALYFRADEDAPYVQRFSIALEKLGPQLARPYAPDNVLEVQDAVGMAVDGCFIGACTTTEEELVL
AALLLEQALRGKAARPASPKRLVVPGDLAIHERMRQAGLWRHYEQAGFRIGPPGCSMCLGVASEKALPGEVWLTSQNRNY
ENRMGPGSHAWLASAATVAASSLEMRVEDPRAALKRIDPSVLERILARKPAGRPIEFPSSEPRLPMTPQPHDGGTRAGTA
SGGRIAGRIQRFGNHVDTDAIIPGEFCHLDEPSELARHAFRYVRPEFLERVQQGRTLIVAGEGWGTGSSREQAVWALAGA
GIKAVIASSYAFIHRRNLVNEALPHLVVTSPAFHALVQEDEELVVDLETGLVEHVASGQRFQAERPSPIARALMQEGGLV
PAIRRLGARALHLGAP

Sequences:

>Translated_656_residues
MNTRPMTLTQKILAHHARRLDRPWVQAGDVLQLRVDWTMASELAWNGMDRTYQALGRPELHDRERVFLAVDHTVDAVTLA
KDVRVQRLVQRSRDFARERRLRHFYDANETILHTKFYRELVQPGQLVLGADSHTTSHGGMGAFAIGLGGADIVAAMVLGE
TWLEVPEAITVEYQGTPAFGIGGKDIILKTLGLLGRNTAALERTVEYRGEAARGFTTDMRFTIANMTAELGGLNGIFEPD
ARVTEWLAMRPSNKDEALYFRADEDAPYVQRFSIALEKLGPQLARPYAPDNVLEVQDAVGMAVDGCFIGACTTTEEELVL
AALLLEQALRGKAARPASPKRLVVPGDLAIHERMRQAGLWRHYEQAGFRIGPPGCSMCLGVASEKALPGEVWLTSQNRNY
ENRMGPGSHAWLASAATVAASSLEMRVEDPRAALKRIDPSVLERILARKPAGRPIEFPSSEPRLPMTPQPHDGGTRAGTA
SGGRIAGRIQRFGNHVDTDAIIPGEFCHLDEPSELARHAFRYVRPEFLERVQQGRTLIVAGEGWGTGSSREQAVWALAGA
GIKAVIASSYAFIHRRNLVNEALPHLVVTSPAFHALVQEDEELVVDLETGLVEHVASGQRFQAERPSPIARALMQEGGLV
PAIRRLGARALHLGAP
>Mature_656_residues
MNTRPMTLTQKILAHHARRLDRPWVQAGDVLQLRVDWTMASELAWNGMDRTYQALGRPELHDRERVFLAVDHTVDAVTLA
KDVRVQRLVQRSRDFARERRLRHFYDANETILHTKFYRELVQPGQLVLGADSHTTSHGGMGAFAIGLGGADIVAAMVLGE
TWLEVPEAITVEYQGTPAFGIGGKDIILKTLGLLGRNTAALERTVEYRGEAARGFTTDMRFTIANMTAELGGLNGIFEPD
ARVTEWLAMRPSNKDEALYFRADEDAPYVQRFSIALEKLGPQLARPYAPDNVLEVQDAVGMAVDGCFIGACTTTEEELVL
AALLLEQALRGKAARPASPKRLVVPGDLAIHERMRQAGLWRHYEQAGFRIGPPGCSMCLGVASEKALPGEVWLTSQNRNY
ENRMGPGSHAWLASAATVAASSLEMRVEDPRAALKRIDPSVLERILARKPAGRPIEFPSSEPRLPMTPQPHDGGTRAGTA
SGGRIAGRIQRFGNHVDTDAIIPGEFCHLDEPSELARHAFRYVRPEFLERVQQGRTLIVAGEGWGTGSSREQAVWALAGA
GIKAVIASSYAFIHRRNLVNEALPHLVVTSPAFHALVQEDEELVVDLETGLVEHVASGQRFQAERPSPIARALMQEGGLV
PAIRRLGARALHLGAP

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=453, Percent_Identity=27.8145695364238, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1786259, Length=429, Percent_Identity=27.972027972028, Blast_Score=149, Evalue=8e-37,
Organism=Escherichia coli, GI87081781, Length=413, Percent_Identity=24.2130750605327, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1787531, Length=405, Percent_Identity=25.1851851851852, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=458, Percent_Identity=27.0742358078603, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32564738, Length=349, Percent_Identity=28.080229226361, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI25149342, Length=290, Percent_Identity=26.2068965517241, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=628, Percent_Identity=26.5923566878981, Blast_Score=190, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6321429, Length=675, Percent_Identity=25.3333333333333, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=679, Percent_Identity=26.2150220913108, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322261, Length=327, Percent_Identity=27.5229357798165, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI281365315, Length=460, Percent_Identity=25.8695652173913, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17864292, Length=460, Percent_Identity=25.8695652173913, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571643, Length=452, Percent_Identity=26.3274336283186, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI161076999, Length=389, Percent_Identity=26.2210796915167, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 72005; Mature: 72005

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTRPMTLTQKILAHHARRLDRPWVQAGDVLQLRVDWTMASELAWNGMDRTYQALGRPEL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHCCCHHHHHHHCCCCC
HDRERVFLAVDHTVDAVTLAKDVRVQRLVQRSRDFARERRLRHFYDANETILHTKFYREL
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
VQPGQLVLGADSHTTSHGGMGAFAIGLGGADIVAAMVLGETWLEVPEAITVEYQGTPAFG
CCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
IGGKDIILKTLGLLGRNTAALERTVEYRGEAARGFTTDMRFTIANMTAELGGLNGIFEPD
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCC
ARVTEWLAMRPSNKDEALYFRADEDAPYVQRFSIALEKLGPQLARPYAPDNVLEVQDAVG
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHC
MAVDGCFIGACTTTEEELVLAALLLEQALRGKAARPASPKRLVVPGDLAIHERMRQAGLW
CEECCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
RHYEQAGFRIGPPGCSMCLGVASEKALPGEVWLTSQNRNYENRMGPGSHAWLASAATVAA
HHHHHCCCEECCCHHHHHHHHHCCCCCCCCEEEECCCCCCHHCCCCCCHHHHHHHHHHHH
SSLEMRVEDPRAALKRIDPSVLERILARKPAGRPIEFPSSEPRLPMTPQPHDGGTRAGTA
HHHHEEECCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SGGRIAGRIQRFGNHVDTDAIIPGEFCHLDEPSELARHAFRYVRPEFLERVQQGRTLIVA
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCEEEEE
GEGWGTGSSREQAVWALAGAGIKAVIASSYAFIHRRNLVNEALPHLVVTSPAFHALVQED
CCCCCCCCCCHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCC
EELVVDLETGLVEHVASGQRFQAERPSPIARALMQEGGLVPAIRRLGARALHLGAP
CCEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCHHCCCCCC
>Mature Secondary Structure
MNTRPMTLTQKILAHHARRLDRPWVQAGDVLQLRVDWTMASELAWNGMDRTYQALGRPEL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHCCCHHHHHHHCCCCC
HDRERVFLAVDHTVDAVTLAKDVRVQRLVQRSRDFARERRLRHFYDANETILHTKFYREL
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
VQPGQLVLGADSHTTSHGGMGAFAIGLGGADIVAAMVLGETWLEVPEAITVEYQGTPAFG
CCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
IGGKDIILKTLGLLGRNTAALERTVEYRGEAARGFTTDMRFTIANMTAELGGLNGIFEPD
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCC
ARVTEWLAMRPSNKDEALYFRADEDAPYVQRFSIALEKLGPQLARPYAPDNVLEVQDAVG
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHC
MAVDGCFIGACTTTEEELVLAALLLEQALRGKAARPASPKRLVVPGDLAIHERMRQAGLW
CEECCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
RHYEQAGFRIGPPGCSMCLGVASEKALPGEVWLTSQNRNYENRMGPGSHAWLASAATVAA
HHHHHCCCEECCCHHHHHHHHHCCCCCCCCEEEECCCCCCHHCCCCCCHHHHHHHHHHHH
SSLEMRVEDPRAALKRIDPSVLERILARKPAGRPIEFPSSEPRLPMTPQPHDGGTRAGTA
HHHHEEECCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SGGRIAGRIQRFGNHVDTDAIIPGEFCHLDEPSELARHAFRYVRPEFLERVQQGRTLIVA
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCEEEEE
GEGWGTGSSREQAVWALAGAGIKAVIASSYAFIHRRNLVNEALPHLVVTSPAFHALVQED
CCCCCCCCCCHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCC
EELVVDLETGLVEHVASGQRFQAERPSPIARALMQEGGLVPAIRRLGARALHLGAP
CCEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]