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The map label for this gene is leuB [H]

Identifier: 108760083

GI number: 108760083

Start: 1499048

End: 1500118

Strand: Reverse

Name: leuB [H]

Synonym: MXAN_1283

Alternate gene names: 108760083

Gene position: 1500118-1499048 (Counterclockwise)

Preceding gene: 108759105

Following gene: 108757641

Centisome position: 16.41

GC content: 66.57

Gene sequence:

>1071_bases
ATGGCAAGAATTGCGGTCATCCCTGGTGATGGCATTGGAATCGAAGTAACCCGAGAGGTCATGCGCATCCTGCAAGTGCT
CGACGCGGAAATGCGGCTCGGGCTCTCACTGGAGGCGTTTGACTTTGGCGCGGAGCGCTTCCTGAGGACAGGGGTGGGGC
TGCCCCCGGGCCAGATGGATGTCTTCCGCACCGAGTTCGATGCCATCCTCCTGGGCGCGATGGGGGACCCGCGCGTGCCA
GACAATGCGCATGCGAGGGAAATCCTGCTGGGCCTGCGCTTCGGGCTCGACCTCTACGTCAATTTCCGGCCCTGCTCTCC
GCTGGCGGAGCGCCTCTCCCCGCTCAAGGGCAAGGGACCGCAAGAGATTGACATGACAATCTTCCGCGAGAACACGGAGG
GCATCTACGGCGCCATCGGCGGGAGCTTCAAGCGGGGCACACTCGACGAGGTCGCCATTGCCGAGGAAATCCATACCCGC
AAGGGGGTGGAGCGAATCATCCGCGCCGCGTTCAAGTGGGCCAGCGCCCATGGCAAGCGGCACGTAGCGCTGGGGGACAA
GTCCAATGCCATTGCCGCCCACGGCCTCTGGCTGCGGACCTTTCGTGAGGTCGCCGCCGAATACCCGCAGATTGAGGCGC
GGCACTACTACGTGGATGCCTTGGCGCTCCAGATGGTGACACGGCCGGAGTCCCTCCAGGTCATCGTGACGACCAATCTC
TTCGGCGACATTCTGACGGACCTGGGCGCGGCCCTGAGCGGTGGGCTTGGCGTTGCGCCCTCCGCCAACCTCAATCCGGA
CTCCACGCCGCTCTTCGAACCCGTGCATGGCTCGGCACCCGAGCTCACCGGCAAGGGGCTGGCCAACCCCGCGGCCATGG
TGCTGAGCGCGGCCCTGATGCTCGAGCACCTCGGTCAGGCTGGGGCCGCCCGGCGTCTGAGCACGGCCGTTCAGCAGACG
GTGGCGGAGGGCCAGTGCACGCGCGACGTGGGGGGAACGCTCTCCACGACGGAGGCGGCCGACGCCATCCTGGACCGGAT
GCTCCAGGGACTCCGGCGGACCCGCACGTAG

Upstream 100 bases:

>100_bases
AAGACCAGAACCCAATAGACTCACATCGCGACGACATACACATGTCAGACAACTTCACACCTTGCCGCGCCCTCGGGATT
CCCGGATTCTCTCTGTCAAC

Downstream 100 bases:

>100_bases
GGGCGGATTGCCCTGCCGCGTGAGGCTGGTGAGCGGAACGCCATCCTCTTGAGGGGACGCCAGCGCGTCCCAAGAAAGGC
GCTGCACATGAACACCCGAC

Product: putative 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MARIAVIPGDGIGIEVTREVMRILQVLDAEMRLGLSLEAFDFGAERFLRTGVGLPPGQMDVFRTEFDAILLGAMGDPRVP
DNAHAREILLGLRFGLDLYVNFRPCSPLAERLSPLKGKGPQEIDMTIFRENTEGIYGAIGGSFKRGTLDEVAIAEEIHTR
KGVERIIRAAFKWASAHGKRHVALGDKSNAIAAHGLWLRTFREVAAEYPQIEARHYYVDALALQMVTRPESLQVIVTTNL
FGDILTDLGAALSGGLGVAPSANLNPDSTPLFEPVHGSAPELTGKGLANPAAMVLSAALMLEHLGQAGAARRLSTAVQQT
VAEGQCTRDVGGTLSTTEAADAILDRMLQGLRRTRT

Sequences:

>Translated_356_residues
MARIAVIPGDGIGIEVTREVMRILQVLDAEMRLGLSLEAFDFGAERFLRTGVGLPPGQMDVFRTEFDAILLGAMGDPRVP
DNAHAREILLGLRFGLDLYVNFRPCSPLAERLSPLKGKGPQEIDMTIFRENTEGIYGAIGGSFKRGTLDEVAIAEEIHTR
KGVERIIRAAFKWASAHGKRHVALGDKSNAIAAHGLWLRTFREVAAEYPQIEARHYYVDALALQMVTRPESLQVIVTTNL
FGDILTDLGAALSGGLGVAPSANLNPDSTPLFEPVHGSAPELTGKGLANPAAMVLSAALMLEHLGQAGAARRLSTAVQQT
VAEGQCTRDVGGTLSTTEAADAILDRMLQGLRRTRT
>Mature_355_residues
ARIAVIPGDGIGIEVTREVMRILQVLDAEMRLGLSLEAFDFGAERFLRTGVGLPPGQMDVFRTEFDAILLGAMGDPRVPD
NAHAREILLGLRFGLDLYVNFRPCSPLAERLSPLKGKGPQEIDMTIFRENTEGIYGAIGGSFKRGTLDEVAIAEEIHTRK
GVERIIRAAFKWASAHGKRHVALGDKSNAIAAHGLWLRTFREVAAEYPQIEARHYYVDALALQMVTRPESLQVIVTTNLF
GDILTDLGAALSGGLGVAPSANLNPDSTPLFEPVHGSAPELTGKGLANPAAMVLSAALMLEHLGQAGAARRLSTAVQQTV
AEGQCTRDVGGTLSTTEAADAILDRMLQGLRRTRT

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=349, Percent_Identity=33.5243553008596, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI4758582, Length=346, Percent_Identity=29.4797687861272, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI28178838, Length=318, Percent_Identity=29.559748427673, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI28178821, Length=348, Percent_Identity=27.2988505747126, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI28178816, Length=345, Percent_Identity=26.9565217391304, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=31.9587628865979, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=40.4494382022472, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI87081683, Length=362, Percent_Identity=35.3591160220994, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787381, Length=397, Percent_Identity=27.7078085642317, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71986051, Length=341, Percent_Identity=34.8973607038123, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17550882, Length=352, Percent_Identity=32.3863636363636, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI25144293, Length=354, Percent_Identity=31.638418079096, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=354, Percent_Identity=29.9435028248588, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6322097, Length=357, Percent_Identity=36.1344537815126, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6319830, Length=361, Percent_Identity=35.4570637119114, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324709, Length=351, Percent_Identity=32.4786324786325, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324291, Length=357, Percent_Identity=32.4929971988796, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24643268, Length=347, Percent_Identity=33.7175792507205, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24643270, Length=347, Percent_Identity=33.7175792507205, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24661184, Length=354, Percent_Identity=31.3559322033898, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI161078635, Length=353, Percent_Identity=27.4787535410765, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078633, Length=353, Percent_Identity=27.4787535410765, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24650122, Length=353, Percent_Identity=27.4787535410765, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078637, Length=353, Percent_Identity=27.4787535410765, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078639, Length=258, Percent_Identity=30.6201550387597, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI281362242, Length=347, Percent_Identity=28.5302593659942, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648872, Length=347, Percent_Identity=28.5302593659942, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI20130355, Length=346, Percent_Identity=23.4104046242775, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38313; Mature: 38181

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIAVIPGDGIGIEVTREVMRILQVLDAEMRLGLSLEAFDFGAERFLRTGVGLPPGQMD
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCHH
VFRTEFDAILLGAMGDPRVPDNAHAREILLGLRFGLDLYVNFRPCSPLAERLSPLKGKGP
HHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCCCC
QEIDMTIFRENTEGIYGAIGGSFKRGTLDEVAIAEEIHTRKGVERIIRAAFKWASAHGKR
CCEEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
HVALGDKSNAIAAHGLWLRTFREVAAEYPQIEARHYYVDALALQMVTRPESLQVIVTTNL
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHH
FGDILTDLGAALSGGLGVAPSANLNPDSTPLFEPVHGSAPELTGKGLANPAAMVLSAALM
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LEHLGQAGAARRLSTAVQQTVAEGQCTRDVGGTLSTTEAADAILDRMLQGLRRTRT
HHHHCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARIAVIPGDGIGIEVTREVMRILQVLDAEMRLGLSLEAFDFGAERFLRTGVGLPPGQMD
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCHH
VFRTEFDAILLGAMGDPRVPDNAHAREILLGLRFGLDLYVNFRPCSPLAERLSPLKGKGP
HHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCCCC
QEIDMTIFRENTEGIYGAIGGSFKRGTLDEVAIAEEIHTRKGVERIIRAAFKWASAHGKR
CCEEEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
HVALGDKSNAIAAHGLWLRTFREVAAEYPQIEARHYYVDALALQMVTRPESLQVIVTTNL
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHH
FGDILTDLGAALSGGLGVAPSANLNPDSTPLFEPVHGSAPELTGKGLANPAAMVLSAALM
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LEHLGQAGAARRLSTAVQQTVAEGQCTRDVGGTLSTTEAADAILDRMLQGLRRTRT
HHHHCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA