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The map label for this gene is purD [H]

Identifier: 108563583

GI number: 108563583

Start: 1224938

End: 1226212

Strand: Reverse

Name: purD [H]

Synonym: HPAG1_1158

Alternate gene names: 108563583

Gene position: 1226212-1224938 (Counterclockwise)

Preceding gene: 108563585

Following gene: 108563582

Centisome position: 76.81

GC content: 39.06

Gene sequence:

>1275_bases
ATGAAAGATAACAATAACTATAATGTTTTAATTGTGGGGAATAAGGGGCGAGAGTATGCTTTGGCTCAAAGGCTTCAGCA
AGATGAGCGAGTGAATGCTTTGTATTTCTGTTTGGGTAATGGTGGCACTCAAGATTTAGGCGAGAATCTGGAATGCGAAC
ATTACGAGCATATCGTGGAATTAGCCCTGAAAAAACAGATCCATTTAGCCATCATTTCAGAAGAAGAGCTTTTGGTTTTA
GGGCTTACAGAAATGCTAGAAAAAGCGGGGATTTTAGTGTTTGGGGCTTCTAAAGAAGCGGCTAAGTTAGAGGCTTCTAA
AAGCTATATGAAAGCTTTTGTTAAAGAATGCGGTATCAAAAGCGCGTCTTACTTTGAAACAAACGATTTAAAAGAAGCTC
TCAATTACATTCAAAACGCTTCTTTCCCATTAGTGATTAAAGCGTTGAATAAAAACACAAGCATTGTCCATCAACAAGAA
GAGGCGTTAAAAATCCTTGAAGACGCTTTCAAACAAAGCAATGAGTCTGTGATCATAGAGCCTTTTTTAGAGGGGTTTGA
GCTTTCAGTTACAGCGCTCATAGCCAATGATGATTTTATCTTGTTGCCCTTTTGCCAAAACTACAAACGCTTATTAGAGG
GGGATAATGGAGTCAATACAGGAGGTATGGGGGCCATCGCTCCTGCAAACTTTTTCTCTAATGAATTAGAAGAGAAAATA
AAAAATCATATCTTTAAACCCACTTTAGAGAAGCTTCAGGCTAATAACACGCCTTTTAAAGGGGTTTTACTCGCTGAAAT
TGTGGTCATAGAAGAAAAGGGCGTTTTAGAGCCGTATTTATTGGATTTTAGCGTGCGTTTTAAAGACATTGAATGCCAGA
CGATTTTACCCCTTTTAGAAAGCTCGCTTTTAGATTTGTGTTTGGCTACAGCCAAAGGGGAATTGCATTCTCTTGAATTG
GTGTTTTCTAAAGAATTTGTGATGAGTGTGGCGCTTGTTTCTAGGAATTACCCCACTAGCTCTTCGCCCAAGCAAACCCT
TTATATTGATCCGGTTGATGAAAAAAAGGGTCATTTGATTTTAGGGGAGGTGGAGCAGGATAATGGCGTGTTTGAAAGCA
GTGGGGGGAGGGTGATCTTTGCCATTGGCAGAGGAAAATCCTTATTAGAAGCCAGAAACCATGCTTATGAAATCGCTCAA
AAGGTGCATTTTGAAGGCATGTTTTATCGCAAGGATATTGGTTTTAAGGTGTTAGATTTGAAAGAATATTCTTAA

Upstream 100 bases:

>100_bases
GAAAATCCTCCTAGCCTTATTAGTGTAGGTTTGTTGAACTTATGCGCATGTAAAAGTAGCGGTCATCTGGGTTTAAAAAC
AACCACATTGTAGGAAAGAC

Downstream 100 bases:

>100_bases
AGGTTAAAGTTTAAGACAAACCAAAGAGTTTGTCTTGTTTGTTTTTGAATGCGCTCTCCAAATTTAGAAAAAGAAGAAAC
TGAAATCATAGAAACACTTC

Product: glycinamide ribonucleotide synthetase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKDNNNYNVLIVGNKGREYALAQRLQQDERVNALYFCLGNGGTQDLGENLECEHYEHIVELALKKQIHLAIISEEELLVL
GLTEMLEKAGILVFGASKEAAKLEASKSYMKAFVKECGIKSASYFETNDLKEALNYIQNASFPLVIKALNKNTSIVHQQE
EALKILEDAFKQSNESVIIEPFLEGFELSVTALIANDDFILLPFCQNYKRLLEGDNGVNTGGMGAIAPANFFSNELEEKI
KNHIFKPTLEKLQANNTPFKGVLLAEIVVIEEKGVLEPYLLDFSVRFKDIECQTILPLLESSLLDLCLATAKGELHSLEL
VFSKEFVMSVALVSRNYPTSSSPKQTLYIDPVDEKKGHLILGEVEQDNGVFESSGGRVIFAIGRGKSLLEARNHAYEIAQ
KVHFEGMFYRKDIGFKVLDLKEYS

Sequences:

>Translated_424_residues
MKDNNNYNVLIVGNKGREYALAQRLQQDERVNALYFCLGNGGTQDLGENLECEHYEHIVELALKKQIHLAIISEEELLVL
GLTEMLEKAGILVFGASKEAAKLEASKSYMKAFVKECGIKSASYFETNDLKEALNYIQNASFPLVIKALNKNTSIVHQQE
EALKILEDAFKQSNESVIIEPFLEGFELSVTALIANDDFILLPFCQNYKRLLEGDNGVNTGGMGAIAPANFFSNELEEKI
KNHIFKPTLEKLQANNTPFKGVLLAEIVVIEEKGVLEPYLLDFSVRFKDIECQTILPLLESSLLDLCLATAKGELHSLEL
VFSKEFVMSVALVSRNYPTSSSPKQTLYIDPVDEKKGHLILGEVEQDNGVFESSGGRVIFAIGRGKSLLEARNHAYEIAQ
KVHFEGMFYRKDIGFKVLDLKEYS
>Mature_424_residues
MKDNNNYNVLIVGNKGREYALAQRLQQDERVNALYFCLGNGGTQDLGENLECEHYEHIVELALKKQIHLAIISEEELLVL
GLTEMLEKAGILVFGASKEAAKLEASKSYMKAFVKECGIKSASYFETNDLKEALNYIQNASFPLVIKALNKNTSIVHQQE
EALKILEDAFKQSNESVIIEPFLEGFELSVTALIANDDFILLPFCQNYKRLLEGDNGVNTGGMGAIAPANFFSNELEEKI
KNHIFKPTLEKLQANNTPFKGVLLAEIVVIEEKGVLEPYLLDFSVRFKDIECQTILPLLESSLLDLCLATAKGELHSLEL
VFSKEFVMSVALVSRNYPTSSSPKQTLYIDPVDEKKGHLILGEVEQDNGVFESSGGRVIFAIGRGKSLLEARNHAYEIAQ
KVHFEGMFYRKDIGFKVLDLKEYS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=427, Percent_Identity=33.0210772833724, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI209869995, Length=427, Percent_Identity=33.0210772833724, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI209869993, Length=427, Percent_Identity=33.0210772833724, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI28416899, Length=427, Percent_Identity=33.0210772833724, Blast_Score=221, Evalue=7e-58,
Organism=Escherichia coli, GI1790438, Length=431, Percent_Identity=28.7703016241299, Blast_Score=205, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17567511, Length=438, Percent_Identity=32.1917808219178, Blast_Score=198, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321203, Length=448, Percent_Identity=30.5803571428571, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI62471619, Length=437, Percent_Identity=30.8924485125858, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24582400, Length=441, Percent_Identity=30.8390022675737, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 47446; Mature: 47446

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDNNNYNVLIVGNKGREYALAQRLQQDERVNALYFCLGNGGTQDLGENLECEHYEHIVE
CCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCHHHHHHHHH
LALKKQIHLAIISEEELLVLGLTEMLEKAGILVFGASKEAAKLEASKSYMKAFVKECGIK
HHHHCCEEEEEECCCCEEEEEHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
SASYFETNDLKEALNYIQNASFPLVIKALNKNTSIVHQQEEALKILEDAFKQSNESVIIE
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEECHHHHHHHHHHHHHCCCCCEEEE
PFLEGFELSVTALIANDDFILLPFCQNYKRLLEGDNGVNTGGMGAIAPANFFSNELEEKI
ECCCCCEEEEEEEEECCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KNHIFKPTLEKLQANNTPFKGVLLAEIVVIEEKGVLEPYLLDFSVRFKDIECQTILPLLE
HHCCCCHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEEEEECCCHHHHHHHHH
SSLLDLCLATAKGELHSLELVFSKEFVMSVALVSRNYPTSSSPKQTLYIDPVDEKKGHLI
HHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEE
LGEVEQDNGVFESSGGRVIFAIGRGKSLLEARNHAYEIAQKVHFEGMFYRKDIGFKVLDL
EEEEECCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEE
KEYS
ECCC
>Mature Secondary Structure
MKDNNNYNVLIVGNKGREYALAQRLQQDERVNALYFCLGNGGTQDLGENLECEHYEHIVE
CCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCHHHHHHHHH
LALKKQIHLAIISEEELLVLGLTEMLEKAGILVFGASKEAAKLEASKSYMKAFVKECGIK
HHHHCCEEEEEECCCCEEEEEHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
SASYFETNDLKEALNYIQNASFPLVIKALNKNTSIVHQQEEALKILEDAFKQSNESVIIE
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEECHHHHHHHHHHHHHCCCCCEEEE
PFLEGFELSVTALIANDDFILLPFCQNYKRLLEGDNGVNTGGMGAIAPANFFSNELEEKI
ECCCCCEEEEEEEEECCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KNHIFKPTLEKLQANNTPFKGVLLAEIVVIEEKGVLEPYLLDFSVRFKDIECQTILPLLE
HHCCCCHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEEEEECCCHHHHHHHHH
SSLLDLCLATAKGELHSLELVFSKEFVMSVALVSRNYPTSSSPKQTLYIDPVDEKKGHLI
HHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEE
LGEVEQDNGVFESSGGRVIFAIGRGKSLLEARNHAYEIAQKVHFEGMFYRKDIGFKVLDL
EEEEECCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEE
KEYS
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]