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The map label for this gene is pnp [H]

Identifier: 108563579

GI number: 108563579

Start: 1219358

End: 1221424

Strand: Reverse

Name: pnp [H]

Synonym: HPAG1_1154

Alternate gene names: 108563579

Gene position: 1221424-1219358 (Counterclockwise)

Preceding gene: 108563580

Following gene: 108563578

Centisome position: 76.51

GC content: 41.22

Gene sequence:

>2067_bases
ATGGATTTTATCACCATCAATTCTAGTAACAAAACCGAAGAGTTCGCTCTCAAACAAGTGGTCAAACAAGCCACCAGCTC
TCTTTTATACCGCTTAGGAAAAACCATCATTTTAGCGAGCGTGTGCGTGGAAAGAGAGCCTGTTAGTGAAGATTTTCTGC
CTTTAGTGGTGCAGTTTTTAGAAAAATCTTATGCAGCCGGAAAGATCCCAGGCGGTTTTGTTAAAAGAGAAGGCAGGGCG
CAAGATTTTGAAATCTTAACCTCTAGGCTCATAGACAGGACTTTACGCCCTTTATTCCCTAAAGATTACCGCTACCCCAC
ACAGATCACTTTAATGGTTTTAAGCCATGATATTGAAAATGACTTGCAGGTTTCTGCTTTAAACGCCGCTTCAGCCGCTC
TCTTTTTGGCCCATATCGCTCCCATTAAAAGCGTGAGCGCTTGCAGGATCGCTAGGATTGGTAACGAATTTATCATTAAC
CCTAACACAAGCCTTTTGAATCAATCCAGTTTGGATTTGTTCGTGTCCGGAACGAAAGAGAGTTTGAACATGATAGAAAT
GCGCTCTTTGGGGCAACAATTAAACGCTTTAGAAGAGCCTTTAATGCTAAAAGCTTTAGAATTGGCTCAAAAAAGTTTGA
AAGAAACTTGCACGCTTTATGAAGAGGTTTTCACGCCTCACCAAAACGAGCTGCTTTTTAAAGAAAGCCAAGGAATAATT
TTTAATGAAAGGCTGTTAGATTTATTAAAAAATCAGTATTTTGATGAAATCATTAAAGGCATTGAAAGTTCTGCTTTGAG
CGAGCGAGAAAATGTTTTCAATGAAATTGCTAAAAAAATCAGCGAAGCCCACTCAGAATTCAGTTTAGAAGAAATTGAAT
GGTCTTTAGAAAAAGTGAAAAAAACTGAGATAAGGCGCATGATTATTCAAGATAAAATCCGCCCGGATAAGCGCGCGTTA
GAAGAAGTGCGGCCCATTTTGATAGAGAGCGATTTGCTCCCTATGGCGCATAGCTCCATTTTATTCACTAGGGGGCAAAC
GCAGAGCTTAGTGGTAGGGGTTTTAGGCACGGATAATGACGCTCAAACCCATGAGAGTTTGGAGCATAAAGCCCCTATCA
AAGAGCGCTTCATGTTTCATTATAATTTCCCTCCTTTTTGCGTGGGCGAAGCGAGTTCTATTGGTGCGGCTTCAAGGCGT
GAATTAGGGCATGGGAATTTGGCTAAAAGAGCCTTAGAAACGAGCATTAAAAATAAAGAGCAGGTGATACGATTGGTTTC
TGAGATTTTAGAAAGCAATGGCTCAAGCTCAATGGCGAGCGTGTGCGCAGGCTCTTTAGCCCTTTATGCAAGCGGTGTGG
AAATTCATGATCTAGTCGCTGGGGTGGCTATGGGCATGGTGAGCGAAGGGCAAGATCACGCTATTTTAAGCGATATTAGC
GGCTTAGAAGACGCCGAAGGCGATATGGATTTTAAGATTGCTGGGAATTTAGAAGGCATTACGGCCATGCAAATGGATAC
CAAAATGAGCGGTATCCAGCTAGAAATTTTATACCAAGCCTTACTCCAAGCCAAAGAAGCGCGGAAACATATTTTAAAAA
TCATGCATGAAGCGAAAGAAAAGATCGTGATCAATTTTTCCCATTTGCCCACGACGGAGATTTTTAATGTCGCGCCCGAT
AAAATTGTAGAAATTATCGGTCAAGGGGGGCGTGTGATTAAAGAGATAGTGGAAAAGTTTGAGGTTAAAATTGATTTGAA
TAAACCGAGCGGTGAAGTGAAAATCATGGGGAATAAAGAGCGCGTTTTAAAGACTAAGGAATTTATTTTAAACTATTTGC
ATTCTTTAGATCAAGAATTGGAGCAATACGCTATTGATGAGGTGCTAGAAGCTCAAGTGAAGCGAATCGTGGATTTTGGG
GCGTTTTTAAGCTTGCCTAAGGGGGGTGAAGGCTTGTTAAGAAAACAAAACATGGACAGGTGTCAAGTGGTTTTAAAAGA
AGGCGATAGCATCAGGTGTAGGGTGATTAGTTTCAATAAGGGTAAAATCGCTTTGGATTTGGCTTAA

Upstream 100 bases:

>100_bases
TGGAGCATCATTATAAAGAACTCAAGCGATTAAGCAATGAAGAAATTGAAAAATACTTGGGCGCTAACAACGCGCCTAAT
TTAAAAAAGGAACATTAAAT

Downstream 100 bases:

>100_bases
AATTTTAAAAAGCGTTTTTTAAAAGCGTTTTTAAGCTAGTTTATATTAGAATAAATCCTTGCTTGTATTTTTGAAAGTAG
AGGAACGATTCGAAATGAAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MDFITINSSNKTEEFALKQVVKQATSSLLYRLGKTIILASVCVEREPVSEDFLPLVVQFLEKSYAAGKIPGGFVKREGRA
QDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDIENDLQVSALNAASAALFLAHIAPIKSVSACRIARIGNEFIIN
PNTSLLNQSSLDLFVSGTKESLNMIEMRSLGQQLNALEEPLMLKALELAQKSLKETCTLYEEVFTPHQNELLFKESQGII
FNERLLDLLKNQYFDEIIKGIESSALSERENVFNEIAKKISEAHSEFSLEEIEWSLEKVKKTEIRRMIIQDKIRPDKRAL
EEVRPILIESDLLPMAHSSILFTRGQTQSLVVGVLGTDNDAQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGAASRR
ELGHGNLAKRALETSIKNKEQVIRLVSEILESNGSSSMASVCAGSLALYASGVEIHDLVAGVAMGMVSEGQDHAILSDIS
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEILYQALLQAKEARKHILKIMHEAKEKIVINFSHLPTTEIFNVAPD
KIVEIIGQGGRVIKEIVEKFEVKIDLNKPSGEVKIMGNKERVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFG
AFLSLPKGGEGLLRKQNMDRCQVVLKEGDSIRCRVISFNKGKIALDLA

Sequences:

>Translated_688_residues
MDFITINSSNKTEEFALKQVVKQATSSLLYRLGKTIILASVCVEREPVSEDFLPLVVQFLEKSYAAGKIPGGFVKREGRA
QDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDIENDLQVSALNAASAALFLAHIAPIKSVSACRIARIGNEFIIN
PNTSLLNQSSLDLFVSGTKESLNMIEMRSLGQQLNALEEPLMLKALELAQKSLKETCTLYEEVFTPHQNELLFKESQGII
FNERLLDLLKNQYFDEIIKGIESSALSERENVFNEIAKKISEAHSEFSLEEIEWSLEKVKKTEIRRMIIQDKIRPDKRAL
EEVRPILIESDLLPMAHSSILFTRGQTQSLVVGVLGTDNDAQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGAASRR
ELGHGNLAKRALETSIKNKEQVIRLVSEILESNGSSSMASVCAGSLALYASGVEIHDLVAGVAMGMVSEGQDHAILSDIS
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEILYQALLQAKEARKHILKIMHEAKEKIVINFSHLPTTEIFNVAPD
KIVEIIGQGGRVIKEIVEKFEVKIDLNKPSGEVKIMGNKERVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFG
AFLSLPKGGEGLLRKQNMDRCQVVLKEGDSIRCRVISFNKGKIALDLA
>Mature_688_residues
MDFITINSSNKTEEFALKQVVKQATSSLLYRLGKTIILASVCVEREPVSEDFLPLVVQFLEKSYAAGKIPGGFVKREGRA
QDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDIENDLQVSALNAASAALFLAHIAPIKSVSACRIARIGNEFIIN
PNTSLLNQSSLDLFVSGTKESLNMIEMRSLGQQLNALEEPLMLKALELAQKSLKETCTLYEEVFTPHQNELLFKESQGII
FNERLLDLLKNQYFDEIIKGIESSALSERENVFNEIAKKISEAHSEFSLEEIEWSLEKVKKTEIRRMIIQDKIRPDKRAL
EEVRPILIESDLLPMAHSSILFTRGQTQSLVVGVLGTDNDAQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGAASRR
ELGHGNLAKRALETSIKNKEQVIRLVSEILESNGSSSMASVCAGSLALYASGVEIHDLVAGVAMGMVSEGQDHAILSDIS
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEILYQALLQAKEARKHILKIMHEAKEKIVINFSHLPTTEIFNVAPD
KIVEIIGQGGRVIKEIVEKFEVKIDLNKPSGEVKIMGNKERVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFG
AFLSLPKGGEGLLRKQNMDRCQVVLKEGDSIRCRVISFNKGKIALDLA

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=691, Percent_Identity=32.7062228654124, Blast_Score=310, Evalue=4e-84,
Organism=Escherichia coli, GI145693187, Length=688, Percent_Identity=35.7558139534884, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI115534063, Length=662, Percent_Identity=30.5135951661631, Blast_Score=268, Evalue=1e-71,
Organism=Drosophila melanogaster, GI281362905, Length=602, Percent_Identity=34.7176079734219, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24651641, Length=602, Percent_Identity=34.7176079734219, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24651643, Length=602, Percent_Identity=34.7176079734219, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI161079377, Length=541, Percent_Identity=35.8595194085028, Blast_Score=288, Evalue=1e-77,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76916; Mature: 76916

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFITINSSNKTEEFALKQVVKQATSSLLYRLGKTIILASVCVEREPVSEDFLPLVVQFL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
EKSYAAGKIPGGFVKREGRAQDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDIEN
HHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHCCCC
DLQVSALNAASAALFLAHIAPIKSVSACRIARIGNEFIINPNTSLLNQSSLDLFVSGTKE
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCEEEEECCHH
SLNMIEMRSLGQQLNALEEPLMLKALELAQKSLKETCTLYEEVFTPHQNELLFKESQGII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCE
FNERLLDLLKNQYFDEIIKGIESSALSERENVFNEIAKKISEAHSEFSLEEIEWSLEKVK
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KTEIRRMIIQDKIRPDKRALEEVRPILIESDLLPMAHSSILFTRGQTQSLVVGVLGTDND
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCCEEEECCCCCEEEEEEEECCCC
AQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGAASRRELGHGNLAKRALETSIKNKE
HHHHHHHHHCCCHHHCEEEEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCHH
QVIRLVSEILESNGSSSMASVCAGSLALYASGVEIHDLVAGVAMGMVSEGQDHAILSDIS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEILYQALLQAKEARKHILKIMHEAKE
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIVINFSHLPTTEIFNVAPDKIVEIIGQGGRVIKEIVEKFEVKIDLNKPSGEVKIMGNKE
EEEEEECCCCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCCEEEECCHH
RVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFGAFLSLPKGGEGLLRKQNMDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHH
CQVVLKEGDSIRCRVISFNKGKIALDLA
HHHHHHCCCCEEEEEEEECCCEEEEEEC
>Mature Secondary Structure
MDFITINSSNKTEEFALKQVVKQATSSLLYRLGKTIILASVCVEREPVSEDFLPLVVQFL
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
EKSYAAGKIPGGFVKREGRAQDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDIEN
HHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHCCCC
DLQVSALNAASAALFLAHIAPIKSVSACRIARIGNEFIINPNTSLLNQSSLDLFVSGTKE
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCEEEEECCHH
SLNMIEMRSLGQQLNALEEPLMLKALELAQKSLKETCTLYEEVFTPHQNELLFKESQGII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCE
FNERLLDLLKNQYFDEIIKGIESSALSERENVFNEIAKKISEAHSEFSLEEIEWSLEKVK
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KTEIRRMIIQDKIRPDKRALEEVRPILIESDLLPMAHSSILFTRGQTQSLVVGVLGTDND
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCCEEEECCCCCEEEEEEEECCCC
AQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGAASRRELGHGNLAKRALETSIKNKE
HHHHHHHHHCCCHHHCEEEEECCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCHH
QVIRLVSEILESNGSSSMASVCAGSLALYASGVEIHDLVAGVAMGMVSEGQDHAILSDIS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEILYQALLQAKEARKHILKIMHEAKE
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIVINFSHLPTTEIFNVAPDKIVEIIGQGGRVIKEIVEKFEVKIDLNKPSGEVKIMGNKE
EEEEEECCCCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCCEEEECCHH
RVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFGAFLSLPKGGEGLLRKQNMDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHH
CQVVLKEGDSIRCRVISFNKGKIALDLA
HHHHHHCCCCEEEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA