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The map label for this gene is ispH

Identifier: 108563417

GI number: 108563417

Start: 1045566

End: 1046390

Strand: Direct

Name: ispH

Synonym: HPAG1_0992

Alternate gene names: 108563417

Gene position: 1045566-1046390 (Clockwise)

Preceding gene: 108563416

Following gene: 108563418

Centisome position: 65.5

GC content: 39.52

Gene sequence:

>825_bases
ATGGAAATTAAAATGGCTAAGGACTATGGTTTTTGTTTTGGCGTCAAAAGAGCGATACAAATCGCTGAAAAAAATCAAAA
CAGCTTGATTTTTGGCTCGCTCATTCATAACGCTAAAGAAATCAATCGTTTGGAAAAAAACTTCAATGTGAAAATTGAAG
AAGACCCTAAAAAAATCCCTAAAAATAAGAGTGTGATCATAAGAACCCATGGCATTCCTAAGCAGGATTTAGAATACTTG
AAAAATAAGGGGGTCAAAATCACTGATGCGACTTGCCCGTATGTGATCAAGCCTCAGCAAATCGTGGAATCCATGAGTAA
AGAAGGGTATCAAATCGTGCTTTTTGGGGATATTAACCACCCTGAAGTCAAGGGCGTGATCAGCTATGCCACTAACCAGG
CTTTAGTCGTCAATTCTTTAGAAGAATTGCAAGAAAAAAAGCTCCAACGAAAAGTAGCTTTAGTCTCACAAACCACCAAA
CAAACCCCAAAACTCTTGCAAATCGCTTCTTATTTGGTGGAAAGATGCACTGAAGTGCGTATTTTCAACACGATTTGCAA
CGCCACTTCTTACAACCAAAAAGCCGCTTTGGATTTGAGTAAGGAAGTGGATATTATGATAGTCGTGGGCGGTAAAACTT
CCTCAAACACCAAACAGCTCTTAAGCATCGCCAAACAGCATTGCAAAGACAGCTACTTGGTAGAAGATGAAAACGAATTA
GAGTTGGCGTGGTTTAAGGATAAAAAATTGTGTGGGATTACCGCTGGGGCTTCCACGCCGGACTGGATTATAGAAAATGT
CAAGCAAAAAATCAGCACGATTTAA

Upstream 100 bases:

>100_bases
ACTTTAGCGTTGCCTTTAGAAATTGATAATTTAGAATGCGCAAACATTTCTTTCCCGCAATTCAAACGCTTACTCAATCT
ATTCAAAAAAGGGAGTTTTA

Downstream 100 bases:

>100_bases
CACATTTTGAAAATTTTCTTTAAAAAAACCGCTAATTGTTAGTTTTTAAACGATTTTGTAAGCCAAAAAAGGATACAATG
CCCTTAAGGATTTTATATTT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MEIKMAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIPKNKSVIIRTHGIPKQDLEYL
KNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINHPEVKGVISYATNQALVVNSLEELQEKKLQRKVALVSQTTK
QTPKLLQIASYLVERCTEVRIFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCKDSYLVEDENEL
ELAWFKDKKLCGITAGASTPDWIIENVKQKISTI

Sequences:

>Translated_274_residues
MEIKMAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIPKNKSVIIRTHGIPKQDLEYL
KNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINHPEVKGVISYATNQALVVNSLEELQEKKLQRKVALVSQTTK
QTPKLLQIASYLVERCTEVRIFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCKDSYLVEDENEL
ELAWFKDKKLCGITAGASTPDWIIENVKQKISTI
>Mature_274_residues
MEIKMAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIPKNKSVIIRTHGIPKQDLEYL
KNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINHPEVKGVISYATNQALVVNSLEELQEKKLQRKVALVSQTTK
QTPKLLQIASYLVERCTEVRIFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCKDSYLVEDENEL
ELAWFKDKKLCGITAGASTPDWIIENVKQKISTI

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=286, Percent_Identity=30.0699300699301, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_HELP2 (B6JMP4)

Other databases:

- EMBL:   CP001217
- RefSeq:   YP_002301652.1
- GeneID:   7010168
- GenomeReviews:   CP001217_GR
- KEGG:   hpp:HPP12_1020
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 30917; Mature: 30917

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKMAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIP
CCEEECCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCHHHCC
KNKSVIIRTHGIPKQDLEYLKNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINH
CCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCEEECHHHHHHHHHHCCCEEEEEECCCC
PEVKGVISYATNQALVVNSLEELQEKKLQRKVALVSQTTKQTPKLLQIASYLVERCTEVR
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCKDSYLVEDENEL
HHHHHHCCCCCCCHHEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEECCCCCE
ELAWFKDKKLCGITAGASTPDWIIENVKQKISTI
EEEEECCCCEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIKMAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIP
CCEEECCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCHHHCC
KNKSVIIRTHGIPKQDLEYLKNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINH
CCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCEEECHHHHHHHHHHCCCEEEEEECCCC
PEVKGVISYATNQALVVNSLEELQEKKLQRKVALVSQTTKQTPKLLQIASYLVERCTEVR
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCKDSYLVEDENEL
HHHHHHCCCCCCCHHEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEECCCCCE
ELAWFKDKKLCGITAGASTPDWIIENVKQKISTI
EEEEECCCCEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA