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The map label for this gene is pheT [H]

Identifier: 108563415

GI number: 108563415

Start: 1041989

End: 1044283

Strand: Direct

Name: pheT [H]

Synonym: HPAG1_0990

Alternate gene names: 108563415

Gene position: 1041989-1044283 (Clockwise)

Preceding gene: 108563414

Following gene: 108563416

Centisome position: 65.27

GC content: 39.39

Gene sequence:

>2295_bases
ATGAAACTGAGTGTCAATGATTTGAGCGTTTTTGTAGATGCACCTAAAGATATAACCAAACTCTGTGAGGATTTGAGCCG
TTTAGGTTTAGAAGTGGAGAGCTGTATGCTTTGTGTCGCTCCTAAAAATGTGGTTGTTGGTAAGGTTTTAGAAAAAGCCC
CCCATAAAAACGCTGAAAAACTCAGCGTGTGTCAAGTGGATGTGGGCAAGGAAGTGTTACAAATCGTGTGTGGGGCTAAA
AATGTCGCGCCAAACCAATTCGTGCCAGTCGCTTTAAATGGGGCGCTAATCGGCTCAACAACCATCGCTAAAACGGAGCT
TAGGGGGGTTGAAAGCTGTGGCATGATTTGCTCTAGCAGCGAATTGGGCTTCCCTAAAATCAATGATGGCATTTTGGAAT
TAGATGAGAGCGTTGGGGAGTTGGTTTTAGGGAAAGAATTAAATGAATACGCCCCTTTCAACACGCATGTTTTAGAAATT
TCATTGACCCCCAATCGTGGGGATTGCTTGAGCGTTTTAGGCGTTGCCAGAGAGATTAGCGCGTTTTATCACACGCCCCT
AAAGCCTATTAAGGCTTTAAATTTTACGCCAAAAAGCGATTGGATCACGCTTAGTGTGGGTGAAAATATCGAATCGCATT
TGGCTTATTATTTGGTTTATAATCATTCTTTAAAAACCCCTTTAAAGGTCAAACTTTCGCTCGCTCATAATAACGCCTTA
AGCGAGAACGATCTAAACAATTTCATAGAATTTAGCACGCATTTTAGTGGGGTAATAATGAACGCTTATAGCCTGAATAT
AACCCCTATGGATTTGAGCGTGAAAAACGATGAAAACAACCTTGAAAGCGTTTATATCAACCATCAAAAACGCTCCACTA
TCGCTATAAAGCATCAAGATCAAAAAGATTTGAGCGAGTGTTTGCTTTTAGAGGCGAGCTATATTGATCCGATAAGCCTG
TCTTTAAAATTACACGCCCTAAAAGATAAAACGCTTCAAAAAGACAATGCCCTTATTTATAGAAGCGCTAGGGGGAGCAA
CCCTAATTTATTGGATGGTTTGAATTTTTTAAGCGCTCATTTGAAAGCGGCAATTTTAGAAGGCAAACAGACCCAACATT
CTTTAAAAGATCGCGCCCTTACATTCCAACTTGAAGACATTACCGAAATTTTGGGGCTTGCTGTAGAGGAAGAAAAAATT
CAAGACATTTTAAAAAATTTAGGCTTTAAAGTTAGCATAAAAGAACCAAACTCAAAACCCCAAATTTTAGAGGTTGTTGT
GCCAAATTTCAGGCATGACATTAAAACGATCCAAGATATTGCTGAAGAAATCTTACGCTTTGTAGGGATTGATAATCTAG
TCTCAAAAACTCTCAATTGCATCAGTAACAAAAATTCAAACCCCCATTATGACACGCACCGCTTTTTTGAAAACCTTAAA
CACAAGGCTCTCGCTTGCGGTTTTAAAGAAGTCATTCATTACGTGTTTTATTCTAAAGAAAAACAGCAAAAACTGGGCTT
TGAAGTTTTAGAAGATCCCCTAGAATTGCAAAACCCTATCACAACGGATCTCAACACCCTAAGAACGAGCCTTGTTTGCG
GGCTTTTAGACGCTAGTTTAAGGAATAAAAATTTAGGGTTTAAAAGCATCGCCCTTTATGAAAAGGGGAGCGTGTATAAC
GCTAAAAGAGAAGAAGTCCAAAAACTAGGCTTTTTAGTAAGCGGCTTACAAAAAAAAGAAAGCTACCCTGACGCTAAAGG
CAAGGCTTGGGATTTTTATTCTTTCGCTGAATGCGTTTCAAGAATTATAGGGGATTTTAGCTTGGAAAAACTAACCGCTC
AAACCCCCATTAACCACCCCTACCAGAGCGCTAAAATCATTCAAAACAATGAAATCATAGGCGTGATCGCTAAAATCCAC
CCTAAAGTGATCCAAGAATGGGATTTGTTTGAAAGCTATTACGCCGAGATAGACGCTTCCAAACTCAAACGCCCTGCCAT
GCTATTAAAACCCTTTAGCATTTACCCTAGTAGTGTTAGGGATTTGACTCTCATCATTGATGAAAACACCGCTTTTAGCA
AGATTGAAAAAGCTCTAAAAGACGCTCAAATCCCTAATTTAAGCGAGATTCTACCCCTTGATATTTTTAAAGAAAGTCAT
AATTCCATAGCCTTAAGCGTGCGTTGCGTGATCCATTCTTTAGAAAAAACCCTGAATGATGAAGAGGTCAATTCAGCCGT
GCAAAAAGCGCTTGAAATTTTAGAAAAAGAATTTAACGCCCGCCTTAAAGGATGA

Upstream 100 bases:

>100_bases
CTTTTGGCATGGGGATTGAAAGATTAGCCATGCTGACTTGCCAAATCAATGATTTGCGCAGTTTCTTTGAAACTGATTTG
AGGGTGTTGGAGAGCTTTTA

Downstream 100 bases:

>100_bases
TATGTGATAGAGCTTGACATTAACGCTAGCGATAAATCGCTCTCGCACAGAGCCGTTATTTTTAGCCTACTCGCTCAAAA
GCCTTGTTTCGTGCGGAATT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MKLSVNDLSVFVDAPKDITKLCEDLSRLGLEVESCMLCVAPKNVVVGKVLEKAPHKNAEKLSVCQVDVGKEVLQIVCGAK
NVAPNQFVPVALNGALIGSTTIAKTELRGVESCGMICSSSELGFPKINDGILELDESVGELVLGKELNEYAPFNTHVLEI
SLTPNRGDCLSVLGVAREISAFYHTPLKPIKALNFTPKSDWITLSVGENIESHLAYYLVYNHSLKTPLKVKLSLAHNNAL
SENDLNNFIEFSTHFSGVIMNAYSLNITPMDLSVKNDENNLESVYINHQKRSTIAIKHQDQKDLSECLLLEASYIDPISL
SLKLHALKDKTLQKDNALIYRSARGSNPNLLDGLNFLSAHLKAAILEGKQTQHSLKDRALTFQLEDITEILGLAVEEEKI
QDILKNLGFKVSIKEPNSKPQILEVVVPNFRHDIKTIQDIAEEILRFVGIDNLVSKTLNCISNKNSNPHYDTHRFFENLK
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTDLNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYN
AKREEVQKLGFLVSGLQKKESYPDAKGKAWDFYSFAECVSRIIGDFSLEKLTAQTPINHPYQSAKIIQNNEIIGVIAKIH
PKVIQEWDLFESYYAEIDASKLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSKIEKALKDAQIPNLSEILPLDIFKESH
NSIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG

Sequences:

>Translated_764_residues
MKLSVNDLSVFVDAPKDITKLCEDLSRLGLEVESCMLCVAPKNVVVGKVLEKAPHKNAEKLSVCQVDVGKEVLQIVCGAK
NVAPNQFVPVALNGALIGSTTIAKTELRGVESCGMICSSSELGFPKINDGILELDESVGELVLGKELNEYAPFNTHVLEI
SLTPNRGDCLSVLGVAREISAFYHTPLKPIKALNFTPKSDWITLSVGENIESHLAYYLVYNHSLKTPLKVKLSLAHNNAL
SENDLNNFIEFSTHFSGVIMNAYSLNITPMDLSVKNDENNLESVYINHQKRSTIAIKHQDQKDLSECLLLEASYIDPISL
SLKLHALKDKTLQKDNALIYRSARGSNPNLLDGLNFLSAHLKAAILEGKQTQHSLKDRALTFQLEDITEILGLAVEEEKI
QDILKNLGFKVSIKEPNSKPQILEVVVPNFRHDIKTIQDIAEEILRFVGIDNLVSKTLNCISNKNSNPHYDTHRFFENLK
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTDLNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYN
AKREEVQKLGFLVSGLQKKESYPDAKGKAWDFYSFAECVSRIIGDFSLEKLTAQTPINHPYQSAKIIQNNEIIGVIAKIH
PKVIQEWDLFESYYAEIDASKLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSKIEKALKDAQIPNLSEILPLDIFKESH
NSIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG
>Mature_764_residues
MKLSVNDLSVFVDAPKDITKLCEDLSRLGLEVESCMLCVAPKNVVVGKVLEKAPHKNAEKLSVCQVDVGKEVLQIVCGAK
NVAPNQFVPVALNGALIGSTTIAKTELRGVESCGMICSSSELGFPKINDGILELDESVGELVLGKELNEYAPFNTHVLEI
SLTPNRGDCLSVLGVAREISAFYHTPLKPIKALNFTPKSDWITLSVGENIESHLAYYLVYNHSLKTPLKVKLSLAHNNAL
SENDLNNFIEFSTHFSGVIMNAYSLNITPMDLSVKNDENNLESVYINHQKRSTIAIKHQDQKDLSECLLLEASYIDPISL
SLKLHALKDKTLQKDNALIYRSARGSNPNLLDGLNFLSAHLKAAILEGKQTQHSLKDRALTFQLEDITEILGLAVEEEKI
QDILKNLGFKVSIKEPNSKPQILEVVVPNFRHDIKTIQDIAEEILRFVGIDNLVSKTLNCISNKNSNPHYDTHRFFENLK
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTDLNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYN
AKREEVQKLGFLVSGLQKKESYPDAKGKAWDFYSFAECVSRIIGDFSLEKLTAQTPINHPYQSAKIIQNNEIIGVIAKIH
PKVIQEWDLFESYYAEIDASKLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSKIEKALKDAQIPNLSEILPLDIFKESH
NSIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=817, Percent_Identity=26.3157894736842, Blast_Score=205, Evalue=1e-53,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 85350; Mature: 85350

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSVNDLSVFVDAPKDITKLCEDLSRLGLEVESCMLCVAPKNVVVGKVLEKAPHKNAEK
CEEECCCEEEEEECCHHHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHHHHHCCCCCCCC
LSVCQVDVGKEVLQIVCGAKNVAPNQFVPVALNGALIGSTTIAKTELRGVESCGMICSSS
EEEEEEHHHHHHHHHHHCCCCCCCCCEEEEEECCEEECCCHHHHHHHHCHHHHCCEECCC
ELGFPKINDGILELDESVGELVLGKELNEYAPFNTHVLEISLTPNRGDCLSVLGVAREIS
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
AFYHTPLKPIKALNFTPKSDWITLSVGENIESHLAYYLVYNHSLKTPLKVKLSLAHNNAL
HHHCCCCCHHHHCCCCCCCCEEEEEECCCHHHHEEEEEEEECCCCCCEEEEEEEECCCCC
SENDLNNFIEFSTHFSGVIMNAYSLNITPMDLSVKNDENNLESVYINHQKRSTIAIKHQD
CCCHHHHHHHHHHHHCEEEEEEEEEEEEEEEEEECCCCCCCEEEEEECCCCCEEEEEECC
QKDLSECLLLEASYIDPISLSLKLHALKDKTLQKDNALIYRSARGSNPNLLDGLNFLSAH
HHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LKAAILEGKQTQHSLKDRALTFQLEDITEILGLAVEEEKIQDILKNLGFKVSIKEPNSKP
HHHHHHCCCHHHHHHHHHEEEEEHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCCCCC
QILEVVVPNFRHDIKTIQDIAEEILRFVGIDNLVSKTLNCISNKNSNPHYDTHRFFENLK
CEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTDLNTLRTSLVCGLLDASL
HHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHH
RNKNLGFKSIALYEKGSVYNAKREEVQKLGFLVSGLQKKESYPDAKGKAWDFYSFAECVS
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
RIIGDFSLEKLTAQTPINHPYQSAKIIQNNEIIGVIAKIHPKVIQEWDLFESYYAEIDAS
HHHCCCHHHHHHHCCCCCCCCCCCEEEECCCEEEEHHHHCHHHHHHHHHHHHHHHHCCHH
KLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSKIEKALKDAQIPNLSEILPLDIFKESH
HCCCCHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHCCHHHHHCCC
NSIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG
CCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLSVNDLSVFVDAPKDITKLCEDLSRLGLEVESCMLCVAPKNVVVGKVLEKAPHKNAEK
CEEECCCEEEEEECCHHHHHHHHHHHHHCCCHHHEEEEECCCHHHHHHHHHHCCCCCCCC
LSVCQVDVGKEVLQIVCGAKNVAPNQFVPVALNGALIGSTTIAKTELRGVESCGMICSSS
EEEEEEHHHHHHHHHHHCCCCCCCCCEEEEEECCEEECCCHHHHHHHHCHHHHCCEECCC
ELGFPKINDGILELDESVGELVLGKELNEYAPFNTHVLEISLTPNRGDCLSVLGVAREIS
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
AFYHTPLKPIKALNFTPKSDWITLSVGENIESHLAYYLVYNHSLKTPLKVKLSLAHNNAL
HHHCCCCCHHHHCCCCCCCCEEEEEECCCHHHHEEEEEEEECCCCCCEEEEEEEECCCCC
SENDLNNFIEFSTHFSGVIMNAYSLNITPMDLSVKNDENNLESVYINHQKRSTIAIKHQD
CCCHHHHHHHHHHHHCEEEEEEEEEEEEEEEEEECCCCCCCEEEEEECCCCCEEEEEECC
QKDLSECLLLEASYIDPISLSLKLHALKDKTLQKDNALIYRSARGSNPNLLDGLNFLSAH
HHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LKAAILEGKQTQHSLKDRALTFQLEDITEILGLAVEEEKIQDILKNLGFKVSIKEPNSKP
HHHHHHCCCHHHHHHHHHEEEEEHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCCCCC
QILEVVVPNFRHDIKTIQDIAEEILRFVGIDNLVSKTLNCISNKNSNPHYDTHRFFENLK
CEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTDLNTLRTSLVCGLLDASL
HHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHH
RNKNLGFKSIALYEKGSVYNAKREEVQKLGFLVSGLQKKESYPDAKGKAWDFYSFAECVS
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
RIIGDFSLEKLTAQTPINHPYQSAKIIQNNEIIGVIAKIHPKVIQEWDLFESYYAEIDAS
HHHCCCHHHHHHHCCCCCCCCCCCEEEECCCEEEEHHHHCHHHHHHHHHHHHHHHHCCHH
KLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSKIEKALKDAQIPNLSEILPLDIFKESH
HCCCCHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHCCHHHHHCCC
NSIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG
CCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]