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The map label for this gene is taqIM [H]

Identifier: 108563317

GI number: 108563317

Start: 927554

End: 928705

Strand: Direct

Name: taqIM [H]

Synonym: HPAG1_0892

Alternate gene names: 108563317

Gene position: 927554-928705 (Clockwise)

Preceding gene: 108563316

Following gene: 108563318

Centisome position: 58.1

GC content: 37.41

Gene sequence:

>1152_bases
TTGGAGAATTTTTTGAATAATTTAGACATTAAAACCTTAGGGCAGGTTTTCACCCCTAAAAAGATAGTGGATTTCATGCT
CACTCTCAAACACAATCATGGGAGTGTTTTAGAGCCAAGCGCGGGCGATGGGAGTTTTTTAAAGCGCTTTAAAAAGGCTG
TAGGGATTGAAATCGATCCTAAAATCTGCCCTAAAAATGCCCTTTGCATGGACTTTTTTGACTACCCTTTAGAAAATCAA
TTTGACACCATTATCGGTAACCCGCCCTATGTCAAGCACAAGGATATTGCGCCAAGCACAAAAGAAAAACTCCATTACAG
CCTTTTTGATGAAAGGAGTAACCTCTACTTGTTTTTCATAGAAAAAGCGATCAAGCATTTAAAACCTAAAGGCGAATTGA
TTTTCATCACCCCAAGGGATTTTTTAAAATCCACTTCTAGCGTGAAATTAAACGAATGGATTTACAAAGAAGGCACGATA
ACGCATTTTTTTGAACTGGGCGATCAAAAGGTTTTCCCAAACGCCATGCCTAATTGCGTGATTTTTCGTTTTTGTAAGGG
CAATTTCAGTAGAATCGCTAACGATGGTTTGCAATTTATGTGCAAAAAAGGCATTTTGTATTTCCTCAACCAATCTTACA
CGCAAAAATTAAGCGAGGTTTTTCAAGTCAAGGTGGGGGCAGTGAGCGGGTGCGATAAGATTTTTAAAAATGAAAAATAC
GGAAATGTAGAATTTGTCACCTCAATCACCAAAAGAACCAATGTTTTAGAAAAAATGGTTTTTGTCAATAAGCCTAATGA
TTATTTACTCCAGCATAAAGACAGCTTGATGCAAAGAAAGATTAAAAAATTCAATGAAAATAATTGGTTTGAGTGGGGGA
GAATGCATCACATATCCCCTAAAAAACGCATTTATGTCAACGCCAAAACGCGCCAAAAAAACCCCTTTTTTATCCACCAA
TGCCCTAATTATGACGGCTCTATTTTAGCGCTATTCCCTTATAACCAAAACTTGGATTTACAAAACCTCTGCGACAAACT
CAACGCTATCAACTGGCAAGAATTAGGCTTTGTGTGCGATGGGCGTTTTTTGTTTTCGCAACGCTCTTTAGAAAACGCTC
TTTTGCCTAAAGACTTTTTAAATCTAGGATAA

Upstream 100 bases:

>100_bases
AGAAATCGTTCAAAGAGACTTTACAGAAAGTAAAAACTTCATCTTAAGCACTTTAGCTAAAAGTGTAACTCTAAGGGCTA
ATATTTATTAAGAGAAGTTT

Downstream 100 bases:

>100_bases
AACTTGTTAGAAACTTTGCAATTAAACCCGGAGCAATTAAAAGCGGCCAAGGCTTTACAAGGGCATAATTTAATCATTGC
GAGTGCTGGCACAGGAAAGA

Product: adenine specific DNA methyltransferase

Products: NA

Alternate protein names: M.TaqI; Adenine-specific methyltransferase TaqI [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MENFLNNLDIKTLGQVFTPKKIVDFMLTLKHNHGSVLEPSAGDGSFLKRFKKAVGIEIDPKICPKNALCMDFFDYPLENQ
FDTIIGNPPYVKHKDIAPSTKEKLHYSLFDERSNLYLFFIEKAIKHLKPKGELIFITPRDFLKSTSSVKLNEWIYKEGTI
THFFELGDQKVFPNAMPNCVIFRFCKGNFSRIANDGLQFMCKKGILYFLNQSYTQKLSEVFQVKVGAVSGCDKIFKNEKY
GNVEFVTSITKRTNVLEKMVFVNKPNDYLLQHKDSLMQRKIKKFNENNWFEWGRMHHISPKKRIYVNAKTRQKNPFFIHQ
CPNYDGSILALFPYNQNLDLQNLCDKLNAINWQELGFVCDGRFLFSQRSLENALLPKDFLNLG

Sequences:

>Translated_383_residues
MENFLNNLDIKTLGQVFTPKKIVDFMLTLKHNHGSVLEPSAGDGSFLKRFKKAVGIEIDPKICPKNALCMDFFDYPLENQ
FDTIIGNPPYVKHKDIAPSTKEKLHYSLFDERSNLYLFFIEKAIKHLKPKGELIFITPRDFLKSTSSVKLNEWIYKEGTI
THFFELGDQKVFPNAMPNCVIFRFCKGNFSRIANDGLQFMCKKGILYFLNQSYTQKLSEVFQVKVGAVSGCDKIFKNEKY
GNVEFVTSITKRTNVLEKMVFVNKPNDYLLQHKDSLMQRKIKKFNENNWFEWGRMHHISPKKRIYVNAKTRQKNPFFIHQ
CPNYDGSILALFPYNQNLDLQNLCDKLNAINWQELGFVCDGRFLFSQRSLENALLPKDFLNLG
>Mature_383_residues
MENFLNNLDIKTLGQVFTPKKIVDFMLTLKHNHGSVLEPSAGDGSFLKRFKKAVGIEIDPKICPKNALCMDFFDYPLENQ
FDTIIGNPPYVKHKDIAPSTKEKLHYSLFDERSNLYLFFIEKAIKHLKPKGELIFITPRDFLKSTSSVKLNEWIYKEGTI
THFFELGDQKVFPNAMPNCVIFRFCKGNFSRIANDGLQFMCKKGILYFLNQSYTQKLSEVFQVKVGAVSGCDKIFKNEKY
GNVEFVTSITKRTNVLEKMVFVNKPNDYLLQHKDSLMQRKIKKFNENNWFEWGRMHHISPKKRIYVNAKTRQKNPFFIHQ
CPNYDGSILALFPYNQNLDLQNLCDKLNAINWQELGFVCDGRFLFSQRSLENALLPKDFLNLG

Specific function: This methylase recognizes the double-stranded sequence TCGA, causes specific methylation on A-4 on both strands and protects the DNA from cleavage by the TaqI endonuclease [H]

COG id: COG0827

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR021188
- InterPro:   IPR023135
- InterPro:   IPR011639 [H]

Pfam domain/function: PF07669 Eco57I [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 44598; Mature: 44598

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENFLNNLDIKTLGQVFTPKKIVDFMLTLKHNHGSVLEPSAGDGSFLKRFKKAVGIEIDP
CCCHHHCCCHHHHHHHCCHHHHHHHHHHEECCCCCEECCCCCCHHHHHHHHHHHCCCCCC
KICPKNALCMDFFDYPLENQFDTIIGNPPYVKHKDIAPSTKEKLHYSLFDERSNLYLFFI
CCCCCCCEEHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEH
EKAIKHLKPKGELIFITPRDFLKSTSSVKLNEWIYKEGTITHFFELGDQKVFPNAMPNCV
HHHHHHCCCCCCEEEECCHHHHCCCCCEEHHHEEECCCCEEEHHHCCCCCCCCCCCCCEE
IFRFCKGNFSRIANDGLQFMCKKGILYFLNQSYTQKLSEVFQVKVGAVSGCDKIFKNEKY
EEEECCCCHHHHHHHHHHHHHHHCHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
GNVEFVTSITKRTNVLEKMVFVNKPNDYLLQHKDSLMQRKIKKFNENNWFEWGRMHHISP
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCC
KKRIYVNAKTRQKNPFFIHQCPNYDGSILALFPYNQNLDLQNLCDKLNAINWQELGFVCD
CCEEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHCCEEEC
GRFLFSQRSLENALLPKDFLNLG
CCCEEHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MENFLNNLDIKTLGQVFTPKKIVDFMLTLKHNHGSVLEPSAGDGSFLKRFKKAVGIEIDP
CCCHHHCCCHHHHHHHCCHHHHHHHHHHEECCCCCEECCCCCCHHHHHHHHHHHCCCCCC
KICPKNALCMDFFDYPLENQFDTIIGNPPYVKHKDIAPSTKEKLHYSLFDERSNLYLFFI
CCCCCCCEEHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEH
EKAIKHLKPKGELIFITPRDFLKSTSSVKLNEWIYKEGTITHFFELGDQKVFPNAMPNCV
HHHHHHCCCCCCEEEECCHHHHCCCCCEEHHHEEECCCCEEEHHHCCCCCCCCCCCCCEE
IFRFCKGNFSRIANDGLQFMCKKGILYFLNQSYTQKLSEVFQVKVGAVSGCDKIFKNEKY
EEEECCCCHHHHHHHHHHHHHHHCHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
GNVEFVTSITKRTNVLEKMVFVNKPNDYLLQHKDSLMQRKIKKFNENNWFEWGRMHHISP
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCC
KKRIYVNAKTRQKNPFFIHQCPNYDGSILALFPYNQNLDLQNLCDKLNAINWQELGFVCD
CCEEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHCCEEEC
GRFLFSQRSLENALLPKDFLNLG
CCCEEHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2827113; 1551602; 7971991 [H]