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The map label for this gene is hemL [H]

Identifier: 108562733

GI number: 108562733

Start: 321213

End: 322505

Strand: Direct

Name: hemL [H]

Synonym: HPAG1_0308

Alternate gene names: 108562733

Gene position: 321213-322505 (Clockwise)

Preceding gene: 108562732

Following gene: 108562734

Centisome position: 20.12

GC content: 43.7

Gene sequence:

>1293_bases
ATGGAGTTGTTGCACAGCATTAATGATTTTAATGAAGCTAAGCAGGTGATCGCTGGGGGGGTCAATTCACCTGTGAGGGC
GTTTAAGAGCGTTAAAGGCACTCCCCCCTTTATTTTAAAGGGTAAGGGGGCGTATCTTTATGATGTGGATAACAACCATT
ATATAGATTTTGTGCAAAGCTGGGGGCCTTTGATTTTTGGGCATGCTGATGAAGAGATTGAAGAAAATATTATTAATACA
TTAAAAAAAGGCACTTCTTTTGGTGCTCCCACAGAATTAGAAACCACTTTAGCTAAGGAAATCATTTCTTGTTATGAAGG
CTTAGATAAGGTGCGTTTAGTGAATAGCGGCACAGAAGCGACCATGAGCGCGATACGACTCGCTAGAGCTTATAGCCAAA
AAGACGATTTGATCAAGTTTGAGGGGTGCTATCATGGGCATAGCGACTCCTTATTGGTGAAAGCGGGTAGCGGGTGCGTT
ACTTTTGGATCGCCTTCTTCTTTAGGCGTGCCGAACGATTTTAGCAAACACACTCTAGTGGCTCGTTATAACGATTTAAA
CTCCACAGAAGAATGCTTCAAAAAAGGCAATGTGGGTTGCGTCATCATTGAACCCATTGCTGGGAATATGGGGTTAGTGC
CGGCTCAAAAAGAGTTTTTATTGGGCTTAAAGGCCTTGTGTGAAAAATACCAAGCGGTGCTGATTTTAGATGAAGTGATG
AGTGGGTTTAGAGCGAGTTTGAGCGGTTCGCAAGAATTTTATGGCGTGGTGCCGGATTTGGTAACCTTTGGTAAGGTGAT
AGGTGCGGGGCTTCCTTTGGCGTGTTTTGGGGGGCGTGCGGAAATTATGGACTTGCTTTCGCCCATTGGAGGCGTGTATC
AAGCAGGCACTTTAAGCGGTAACCCCCTAGCGGTGTGTGCGGGGTTGAGCGCGCTTTATAAAATCAAAAGAGACAAAACC
CTTTATACCCGCTTAAACGCTTTAGCTATTCGTTTGACTCAAGGCTTAAAAAAGAGCGCTCAAAGCTATAACATCGCTTT
AGAAACGCTCAACAGAGGGAGCATGTTTGGTTTTTTCTTTAACGAAAATGCGGTGTGCGATTTTGATGACGCTTTAAAAA
GCGACACGGAGATGTTTGCAAAATTCCACCAAAAAATGCTCTTTAAGGGCGTGTATTTGGCGTGCTCAAGCTTTGAAACC
GGCTTTATTTGTGAGCCTATGACTGAAGAGATGATTGATTTAGTGGTTGCAAAGGCTGATGAAAGTTTTGATGAAATCAT
AAAGGGCGTGTGA

Upstream 100 bases:

>100_bases
AGCGAGCTTGGCTAAACGATGCGAGAGTTTTCATGAGGGCTTGACTTGGTCGCAAGTGGAAATCCAATTTGAAAGCATGA
TTAAGGGATAATTTAAAATC

Downstream 100 bases:

>100_bases
ATTTTTGAAAAAGCCAAAGTATTATAAATTCATAGAGGGGGCGAATTATTTGAGCTTAGGGCTGTCTATGGTGGTAGCGA
TCCTTATGGGCGTGGCTATA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MELLHSINDFNEAKQVIAGGVNSPVRAFKSVKGTPPFILKGKGAYLYDVDNNHYIDFVQSWGPLIFGHADEEIEENIINT
LKKGTSFGAPTELETTLAKEIISCYEGLDKVRLVNSGTEATMSAIRLARAYSQKDDLIKFEGCYHGHSDSLLVKAGSGCV
TFGSPSSLGVPNDFSKHTLVARYNDLNSTEECFKKGNVGCVIIEPIAGNMGLVPAQKEFLLGLKALCEKYQAVLILDEVM
SGFRASLSGSQEFYGVVPDLVTFGKVIGAGLPLACFGGRAEIMDLLSPIGGVYQAGTLSGNPLAVCAGLSALYKIKRDKT
LYTRLNALAIRLTQGLKKSAQSYNIALETLNRGSMFGFFFNENAVCDFDDALKSDTEMFAKFHQKMLFKGVYLACSSFET
GFICEPMTEEMIDLVVAKADESFDEIIKGV

Sequences:

>Translated_430_residues
MELLHSINDFNEAKQVIAGGVNSPVRAFKSVKGTPPFILKGKGAYLYDVDNNHYIDFVQSWGPLIFGHADEEIEENIINT
LKKGTSFGAPTELETTLAKEIISCYEGLDKVRLVNSGTEATMSAIRLARAYSQKDDLIKFEGCYHGHSDSLLVKAGSGCV
TFGSPSSLGVPNDFSKHTLVARYNDLNSTEECFKKGNVGCVIIEPIAGNMGLVPAQKEFLLGLKALCEKYQAVLILDEVM
SGFRASLSGSQEFYGVVPDLVTFGKVIGAGLPLACFGGRAEIMDLLSPIGGVYQAGTLSGNPLAVCAGLSALYKIKRDKT
LYTRLNALAIRLTQGLKKSAQSYNIALETLNRGSMFGFFFNENAVCDFDDALKSDTEMFAKFHQKMLFKGVYLACSSFET
GFICEPMTEEMIDLVVAKADESFDEIIKGV
>Mature_430_residues
MELLHSINDFNEAKQVIAGGVNSPVRAFKSVKGTPPFILKGKGAYLYDVDNNHYIDFVQSWGPLIFGHADEEIEENIINT
LKKGTSFGAPTELETTLAKEIISCYEGLDKVRLVNSGTEATMSAIRLARAYSQKDDLIKFEGCYHGHSDSLLVKAGSGCV
TFGSPSSLGVPNDFSKHTLVARYNDLNSTEECFKKGNVGCVIIEPIAGNMGLVPAQKEFLLGLKALCEKYQAVLILDEVM
SGFRASLSGSQEFYGVVPDLVTFGKVIGAGLPLACFGGRAEIMDLLSPIGGVYQAGTLSGNPLAVCAGLSALYKIKRDKT
LYTRLNALAIRLTQGLKKSAQSYNIALETLNRGSMFGFFFNENAVCDFDDALKSDTEMFAKFHQKMLFKGVYLACSSFET
GFICEPMTEEMIDLVVAKADESFDEIIKGV

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=314, Percent_Identity=26.7515923566879, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI13994255, Length=363, Percent_Identity=26.4462809917355, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI284507298, Length=228, Percent_Identity=26.7543859649123, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786349, Length=424, Percent_Identity=50.2358490566038, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1789016, Length=386, Percent_Identity=29.0155440414508, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787560, Length=378, Percent_Identity=30.6878306878307, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788044, Length=284, Percent_Identity=33.8028169014084, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789759, Length=333, Percent_Identity=28.8288288288288, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI145693181, Length=393, Percent_Identity=26.4631043256997, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71992977, Length=336, Percent_Identity=29.1666666666667, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25144271, Length=293, Percent_Identity=25.938566552901, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25144274, Length=203, Percent_Identity=27.5862068965517, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=341, Percent_Identity=28.1524926686217, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324432, Length=347, Percent_Identity=26.5129682997118, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357415, Length=295, Percent_Identity=25.0847457627119, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574759, Length=367, Percent_Identity=26.9754768392371, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI161085790, Length=367, Percent_Identity=26.9754768392371, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46729; Mature: 46729

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLHSINDFNEAKQVIAGGVNSPVRAFKSVKGTPPFILKGKGAYLYDVDNNHYIDFVQS
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEECCCEEEEECCCCCEEHHHHC
WGPLIFGHADEEIEENIINTLKKGTSFGAPTELETTLAKEIISCYEGLDKVRLVNSGTEA
CCCEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHEEEECCCHHH
TMSAIRLARAYSQKDDLIKFEGCYHGHSDSLLVKAGSGCVTFGSPSSLGVPNDFSKHTLV
HHHHHHHHHHHCCCCCCEEEECEECCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCEEE
ARYNDLNSTEECFKKGNVGCVIIEPIAGNMGLVPAQKEFLLGLKALCEKYQAVLILDEVM
EEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SGFRASLSGSQEFYGVVPDLVTFGKVIGAGLPLACFGGRAEIMDLLSPIGGVYQAGTLSG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCEECCCCCC
NPLAVCAGLSALYKIKRDKTLYTRLNALAIRLTQGLKKSAQSYNIALETLNRGSMFGFFF
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEE
NENAVCDFDDALKSDTEMFAKFHQKMLFKGVYLACSSFETGFICEPMTEEMIDLVVAKAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
ESFDEIIKGV
CCHHHHHCCC
>Mature Secondary Structure
MELLHSINDFNEAKQVIAGGVNSPVRAFKSVKGTPPFILKGKGAYLYDVDNNHYIDFVQS
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEECCCEEEEECCCCCEEHHHHC
WGPLIFGHADEEIEENIINTLKKGTSFGAPTELETTLAKEIISCYEGLDKVRLVNSGTEA
CCCEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHEEEECCCHHH
TMSAIRLARAYSQKDDLIKFEGCYHGHSDSLLVKAGSGCVTFGSPSSLGVPNDFSKHTLV
HHHHHHHHHHHCCCCCCEEEECEECCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCEEE
ARYNDLNSTEECFKKGNVGCVIIEPIAGNMGLVPAQKEFLLGLKALCEKYQAVLILDEVM
EEECCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SGFRASLSGSQEFYGVVPDLVTFGKVIGAGLPLACFGGRAEIMDLLSPIGGVYQAGTLSG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCEECCCCCC
NPLAVCAGLSALYKIKRDKTLYTRLNALAIRLTQGLKKSAQSYNIALETLNRGSMFGFFF
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEE
NENAVCDFDDALKSDTEMFAKFHQKMLFKGVYLACSSFETGFICEPMTEEMIDLVVAKAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
ESFDEIIKGV
CCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA