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The map label for this gene is nth [H]

Identifier: 103488222

GI number: 103488222

Start: 2896124

End: 2896792

Strand: Reverse

Name: nth [H]

Synonym: Sala_2745

Alternate gene names: 103488222

Gene position: 2896792-2896124 (Counterclockwise)

Preceding gene: 103488224

Following gene: 103488215

Centisome position: 86.6

GC content: 62.63

Gene sequence:

>669_bases
ATGAAACGCAGCGACATCTTTGAATTCTACCGCCGCCTCGCCGAACTGAACCCCAGTCCCGAGACCGAGCTGCAATTCGG
CAACATCTATCAGCTGCTCGTCGCGGTCGTATTGTCGGCGCAGGCAACCGACGTCGGGGTGAACAAGGCGACGCGCAAAT
TGTTCGAAACGGTGAAGACGCCGCAACAGATGCTCGATCTGGGCGAGGAGGGCCTCAAACAGCATATCAGGACGATCGGG
CTGTTCAACGCCAAGGCGAAAAATGTCATTGCGCTCAGCGAAATGCTGATCCGCGACCATGGCGGCGAAGTGCCCGCCGA
CCGCGATGCGCTCACAAAGCTGCCCGGCGTCGGACGCAAGACCGCCAATGTCGTGATGAACTGCGCTTTCGGGGCGGAGA
CCTTTGCGGTCGATACGCACATCTTTCGCGTCGGCAACCGCACCGGGCTGGCGCCGGGGAATACGGTGCTCGCGGTCGAG
AAAAAACTCGAGAAAGGCACGCCCGCGCCCTTCCGCGTCGGCGCGCACCACTGGCTGATTCTCCACGGCCGCTATATCTG
CAAGGCACGCACCCCCGAATGCTGGCGCTGCCCGGTCGCGGACCTTTGCCGGTACCAGCCCAAGACGGCGGCGCCGACGT
TGCGGAAAAAGACAGGGGCGCGGGAGTGA

Upstream 100 bases:

>100_bases
AGAGTCAAATGCGACGGCTTGGAAGGCGGCGGGCAAAGGCGCTAGGGTCAGGACCCCAGGGAATGCGGGCGGCGCTTCGG
CCGCGCGCCGGAGCCAAGCG

Downstream 100 bases:

>100_bases
CCTCCCGCGCCCCGTTTTTTTGGACCAAAGGCCTGTCGGGCTAGAAGTTGACCCGCACGCCGACGCGGACGCCGTACAGC
GACACGCGGCTGGTGAGCAC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKRSDIFEFYRRLAELNPSPETELQFGNIYQLLVAVVLSAQATDVGVNKATRKLFETVKTPQQMLDLGEEGLKQHIRTIG
LFNAKAKNVIALSEMLIRDHGGEVPADRDALTKLPGVGRKTANVVMNCAFGAETFAVDTHIFRVGNRTGLAPGNTVLAVE
KKLEKGTPAPFRVGAHHWLILHGRYICKARTPECWRCPVADLCRYQPKTAAPTLRKKTGARE

Sequences:

>Translated_222_residues
MKRSDIFEFYRRLAELNPSPETELQFGNIYQLLVAVVLSAQATDVGVNKATRKLFETVKTPQQMLDLGEEGLKQHIRTIG
LFNAKAKNVIALSEMLIRDHGGEVPADRDALTKLPGVGRKTANVVMNCAFGAETFAVDTHIFRVGNRTGLAPGNTVLAVE
KKLEKGTPAPFRVGAHHWLILHGRYICKARTPECWRCPVADLCRYQPKTAAPTLRKKTGARE
>Mature_222_residues
MKRSDIFEFYRRLAELNPSPETELQFGNIYQLLVAVVLSAQATDVGVNKATRKLFETVKTPQQMLDLGEEGLKQHIRTIG
LFNAKAKNVIALSEMLIRDHGGEVPADRDALTKLPGVGRKTANVVMNCAFGAETFAVDTHIFRVGNRTGLAPGNTVLAVE
KKLEKGTPAPFRVGAHHWLILHGRYICKARTPECWRCPVADLCRYQPKTAAPTLRKKTGARE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=30.5084745762712, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=59.1346153846154, Blast_Score=253, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI17554540, Length=191, Percent_Identity=31.413612565445, Blast_Score=90, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45550361, Length=195, Percent_Identity=29.7435897435897, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24620; Mature: 24620

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSDIFEFYRRLAELNPSPETELQFGNIYQLLVAVVLSAQATDVGVNKATRKLFETVKT
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
PQQMLDLGEEGLKQHIRTIGLFNAKAKNVIALSEMLIRDHGGEVPADRDALTKLPGVGRK
HHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCH
TANVVMNCAFGAETFAVDTHIFRVGNRTGLAPGNTVLAVEKKLEKGTPAPFRVGAHHWLI
HHHHHHHHCCCCCCEEEHHHHEEECCCCCCCCCCEEEEEEHHHHCCCCCCEEECCCEEEE
LHGRYICKARTPECWRCPVADLCRYQPKTAAPTLRKKTGARE
EECEEEEECCCCCCCCCCHHHHHCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MKRSDIFEFYRRLAELNPSPETELQFGNIYQLLVAVVLSAQATDVGVNKATRKLFETVKT
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
PQQMLDLGEEGLKQHIRTIGLFNAKAKNVIALSEMLIRDHGGEVPADRDALTKLPGVGRK
HHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCH
TANVVMNCAFGAETFAVDTHIFRVGNRTGLAPGNTVLAVEKKLEKGTPAPFRVGAHHWLI
HHHHHHHHCCCCCCEEEHHHHEEECCCCCCCCCCEEEEEEHHHHCCCCCCEEECCCEEEE
LHGRYICKARTPECWRCPVADLCRYQPKTAAPTLRKKTGARE
EECEEEEECCCCCCCCCCHHHHHCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]