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The map label for this gene is leuD

Identifier: 103487612

GI number: 103487612

Start: 2236208

End: 2236801

Strand: Direct

Name: leuD

Synonym: Sala_2131

Alternate gene names: 103487612

Gene position: 2236208-2236801 (Clockwise)

Preceding gene: 103487611

Following gene: 103487613

Centisome position: 66.85

GC content: 64.81

Gene sequence:

>594_bases
ATGACGCCGCTGAACCAGGTCGAGGGCCGCGCCATCCCGCTGGGCCTCAAAAATATCGACACCGACGTCATCATCCCTGC
GCACTGGCTGAAGACCACGACGCGCGAGGGCATGGGACGCGGCGCGTTCGAAAGCCTGCGCGCCGATCCCGACAATCTGT
TCGACCGCCCCGAATACAGGCAAGCCCCGATCCTGATCGCGGGCGACAATTTCGGATGCGGGTCGAGCCGCGAACATGCC
GCCTGGGCGCTCGGCGACCTCGGTATCCGCGTCGTGATCGCGCCGAGCTATTCGGACATTTTTTCGGGCAATGCGGTGAA
GAACGGCATCCTGCCGGTCGTGCTGCCGCAGGCGGCGATCGACCGGCTTCTCGAAGTGGCGGCGACCGATCCGGTCCGCA
TCGACCTCGAGCATCAGACGGTGACGACGCCGTTCCAGGACCGCTTCACCTTCGAGATCGACCCGTTCCGCAAGATGTGC
CTGCTCGAAGGGCTCGACGAGATTTCGCTGACCGAAAAGAGCGATGCGGCGATCGGCGCCTATGAAGACAGGCTCGATGC
CGAGCGTCCGTGGATGCGCGCCGTCGTCGGGTAG

Upstream 100 bases:

>100_bases
TTCAAGGGGCTGGCGAAGAATCTGGGCGCGACGGTGATCATCCAGCACGAGCCGCGCGACGTCGCCAAGCTGCCCCCCTT
TCCCAAGGCGGCCGAATGAC

Downstream 100 bases:

>100_bases
GACACCGAAGAGGAGAAGATGTGATGAAGGCTCTTTTGTCGACCGCAACCGGCGGCCCCGAAACGCTGGTCCTTGGCGAA
CTGCCGCGCCCGAGCGCGGG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MTPLNQVEGRAIPLGLKNIDTDVIIPAHWLKTTTREGMGRGAFESLRADPDNLFDRPEYRQAPILIAGDNFGCGSSREHA
AWALGDLGIRVVIAPSYSDIFSGNAVKNGILPVVLPQAAIDRLLEVAATDPVRIDLEHQTVTTPFQDRFTFEIDPFRKMC
LLEGLDEISLTEKSDAAIGAYEDRLDAERPWMRAVVG

Sequences:

>Translated_197_residues
MTPLNQVEGRAIPLGLKNIDTDVIIPAHWLKTTTREGMGRGAFESLRADPDNLFDRPEYRQAPILIAGDNFGCGSSREHA
AWALGDLGIRVVIAPSYSDIFSGNAVKNGILPVVLPQAAIDRLLEVAATDPVRIDLEHQTVTTPFQDRFTFEIDPFRKMC
LLEGLDEISLTEKSDAAIGAYEDRLDAERPWMRAVVG
>Mature_196_residues
TPLNQVEGRAIPLGLKNIDTDVIIPAHWLKTTTREGMGRGAFESLRADPDNLFDRPEYRQAPILIAGDNFGCGSSREHAA
WALGDLGIRVVIAPSYSDIFSGNAVKNGILPVVLPQAAIDRLLEVAATDPVRIDLEHQTVTTPFQDRFTFEIDPFRKMCL
LEGLDEISLTEKSDAAIGAYEDRLDAERPWMRAVVG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=187, Percent_Identity=44.9197860962567, Blast_Score=162, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321429, Length=196, Percent_Identity=43.3673469387755, Blast_Score=151, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_SPHAL (Q1GR82)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617173.1
- ProteinModelPortal:   Q1GR82
- SMR:   Q1GR82
- GeneID:   4080106
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2131
- NMPDR:   fig|317655.9.peg.2058
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   SALA317655:SALA_2131-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21716; Mature: 21585

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLNQVEGRAIPLGLKNIDTDVIIPAHWLKTTTREGMGRGAFESLRADPDNLFDRPEYR
CCCCCCCCCCEEEEECCCCCCCEEECHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCCCCC
QAPILIAGDNFGCGSSREHAAWALGDLGIRVVIAPSYSDIFSGNAVKNGILPVVLPQAAI
CCCEEEECCCCCCCCCCCCCEEEECCCCEEEEEECCCHHHHCCCCCCCCCCEEEECHHHH
DRLLEVAATDPVRIDLEHQTVTTPFQDRFTFEIDPFRKMCLLEGLDEISLTEKSDAAIGA
HHHHHHHCCCCEEEEECCCEECCCCCCCEEEEECHHHHHHHHCCCCCCCCCCCCCCCCCC
YEDRLDAERPWMRAVVG
HHHHCCCCCCHHHHHCC
>Mature Secondary Structure 
TPLNQVEGRAIPLGLKNIDTDVIIPAHWLKTTTREGMGRGAFESLRADPDNLFDRPEYR
CCCCCCCCCEEEEECCCCCCCEEECHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCCCCC
QAPILIAGDNFGCGSSREHAAWALGDLGIRVVIAPSYSDIFSGNAVKNGILPVVLPQAAI
CCCEEEECCCCCCCCCCCCCEEEECCCCEEEEEECCCHHHHCCCCCCCCCCEEEECHHHH
DRLLEVAATDPVRIDLEHQTVTTPFQDRFTFEIDPFRKMCLLEGLDEISLTEKSDAAIGA
HHHHHHHCCCCEEEEECCCEECCCCCCCEEEEECHHHHHHHHCCCCCCCCCCCCCCCCCC
YEDRLDAERPWMRAVVG
HHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA