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The map label for this gene is emrB [H]

Identifier: 103487564

GI number: 103487564

Start: 2188781

End: 2190370

Strand: Direct

Name: emrB [H]

Synonym: Sala_2083

Alternate gene names: 103487564

Gene position: 2188781-2190370 (Clockwise)

Preceding gene: 103487563

Following gene: 103487566

Centisome position: 65.43

GC content: 66.79

Gene sequence:

>1590_bases
ATGGCCTCCCGCTCCCTCCCCCGCCGTCCTGCTGCCGCGGCGCTCCCCGCTGACGATATGATCCCGCTCCACGAACGCGT
GCGCTACCGCGGCCTGCTCACCGTCGCCGTCATGGGCGCGTCGATCATGCAGATCCTCGACACGACGATCGCCAATGTCG
CCATCCCGCACATGCAATCGGCCTTGGGCGCGACGAGCGAAACGGTGACGTGGGTGCTCACCAGCTATATCCTCGCAAGC
GCGATCGCGATGCCGATCACCGGCTGGCTCGCCGACCGCATCGGGCGGCGCGAGCTGTTCCTTGCCGCGATCGCGGGGTT
CATCGTCGCGTCGATGGCGTGCGGCGCGGCGCAATCGCTCGAACAGATGGTCGCCTTCCGCTTTTTGCAGGGTATTTTCG
CGGCCTTCATCGGCCCGCTGTCGCAGTCGGTGATGCTCGACATCAACCCGCCCGAGCGCCATGCGCGCGCGATGTCGATC
TGGGGCATGGGGATCATGATCGGACCCATTCTCGGCCCCGTGCTCGGCGGCTGGCTGACCGAAAGCGCGAACTGGCGCTG
GGTCTTTTACGTCAACCTGCCCGTCGGGCTGGTCACGCTCGCGCTGATGTGGGCGCTGCTGCCCGCGATGCGCCGGACAA
GCCGCAAGTTCGATCTCTTCGGCTTTTCGATGCTCGCGCTGGGGCTCGCCGCGCTGCAACTGATGCTCGACCGCGGCGCG
CATCTCGACTGGTTCGACAGCATCGAAATCTGGATCGAACTCGGCGTCGCGACGGCCTGCCTCTGGATGTTCTTCGTCCA
TCTCTTCACCGCGCGCGCGCCGCTGTTCAGCCGCGCGATGCTCGCCGACCGCAACCTCGTCACCGCGATGGGCTTCATGA
TCGTCATCGGCATCGTGATGTTCGCGTCGATGGCGCTGCTGCCGCCGATGCTGCAAAATCTCTTCGGCTGGCCGGTGATC
GACACGGGGCTGGTGCTCGCGGTGCGCGGCGTCGGCATCCTCGCGAGCATGTGGGTCGCGGGGCAGTTGCTGGGCAAGGT
CGACGCGCGCTGGCTCGTCGGCACCGGCCTTGCCATCGCCGCCTATTCGCTGTGGCAGATGAGCCACTGGTCGCTCGCCA
TGGGGATGCAGCCGGTGATCGTCAGCGGGCTGGTGCAGGGGCTGGGCATGGGGCTGATCTTCATTCCGCTCAACACCATG
GCCTTTGCAACGATCGCGCCGCAGCACCGCACCGACGGGTCGAGCCTGCTGAACCTGCTCCGCAGCCTCGGCGCCTCGGT
CGGCATTTCGGTGGTGACCACCCTGCTCGGCATCAATATCCAGACCAGCCATCAGGATCTCGCCGCGCATGTCACCAACA
GCTCGGTCGCGCTCATCGACCCCTCGACCGCCGACCGCTTCGGCGTCGTCGGCGACACCGCGCTGGCGATGGTCAACGCC
GAGATCAACCGGCAGGCGGCGATGGTCGCCTATATCGACGATTTCTGGCTGATGATGTGGGTGACTTTGCTTTCGGTCCC
GCTCGTCCTCCTGCTCCGCCCGCCCAGGGCCGGCGCGCCCAAGGCCTCGGCGGCCGACATGGGGCATTGA

Upstream 100 bases:

>100_bases
GGGCGATGACACCGCCGTCGCGGCCCCTCCACCCCTCGCTGCGCGAGCGGTCCCCCTCCCCATCGCAAGTCGATGGGGAG
GATTTTAACGCTGTTCCCTA

Downstream 100 bases:

>100_bases
GACGCCGCGACTCCCGCAAAGGCAGGCCCCGGAGACGGGGAGAGAGAGGACCGCTCCGGACGGCCCGCCTTCGCAGGAAA
GGATGCTCAGGCCGCAGCCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MASRSLPRRPAAAALPADDMIPLHERVRYRGLLTVAVMGASIMQILDTTIANVAIPHMQSALGATSETVTWVLTSYILAS
AIAMPITGWLADRIGRRELFLAAIAGFIVASMACGAAQSLEQMVAFRFLQGIFAAFIGPLSQSVMLDINPPERHARAMSI
WGMGIMIGPILGPVLGGWLTESANWRWVFYVNLPVGLVTLALMWALLPAMRRTSRKFDLFGFSMLALGLAALQLMLDRGA
HLDWFDSIEIWIELGVATACLWMFFVHLFTARAPLFSRAMLADRNLVTAMGFMIVIGIVMFASMALLPPMLQNLFGWPVI
DTGLVLAVRGVGILASMWVAGQLLGKVDARWLVGTGLAIAAYSLWQMSHWSLAMGMQPVIVSGLVQGLGMGLIFIPLNTM
AFATIAPQHRTDGSSLLNLLRSLGASVGISVVTTLLGINIQTSHQDLAAHVTNSSVALIDPSTADRFGVVGDTALAMVNA
EINRQAAMVAYIDDFWLMMWVTLLSVPLVLLLRPPRAGAPKASAADMGH

Sequences:

>Translated_529_residues
MASRSLPRRPAAAALPADDMIPLHERVRYRGLLTVAVMGASIMQILDTTIANVAIPHMQSALGATSETVTWVLTSYILAS
AIAMPITGWLADRIGRRELFLAAIAGFIVASMACGAAQSLEQMVAFRFLQGIFAAFIGPLSQSVMLDINPPERHARAMSI
WGMGIMIGPILGPVLGGWLTESANWRWVFYVNLPVGLVTLALMWALLPAMRRTSRKFDLFGFSMLALGLAALQLMLDRGA
HLDWFDSIEIWIELGVATACLWMFFVHLFTARAPLFSRAMLADRNLVTAMGFMIVIGIVMFASMALLPPMLQNLFGWPVI
DTGLVLAVRGVGILASMWVAGQLLGKVDARWLVGTGLAIAAYSLWQMSHWSLAMGMQPVIVSGLVQGLGMGLIFIPLNTM
AFATIAPQHRTDGSSLLNLLRSLGASVGISVVTTLLGINIQTSHQDLAAHVTNSSVALIDPSTADRFGVVGDTALAMVNA
EINRQAAMVAYIDDFWLMMWVTLLSVPLVLLLRPPRAGAPKASAADMGH
>Mature_528_residues
ASRSLPRRPAAAALPADDMIPLHERVRYRGLLTVAVMGASIMQILDTTIANVAIPHMQSALGATSETVTWVLTSYILASA
IAMPITGWLADRIGRRELFLAAIAGFIVASMACGAAQSLEQMVAFRFLQGIFAAFIGPLSQSVMLDINPPERHARAMSIW
GMGIMIGPILGPVLGGWLTESANWRWVFYVNLPVGLVTLALMWALLPAMRRTSRKFDLFGFSMLALGLAALQLMLDRGAH
LDWFDSIEIWIELGVATACLWMFFVHLFTARAPLFSRAMLADRNLVTAMGFMIVIGIVMFASMALLPPMLQNLFGWPVID
TGLVLAVRGVGILASMWVAGQLLGKVDARWLVGTGLAIAAYSLWQMSHWSLAMGMQPVIVSGLVQGLGMGLIFIPLNTMA
FATIAPQHRTDGSSLLNLLRSLGASVGISVVTTLLGINIQTSHQDLAAHVTNSSVALIDPSTADRFGVVGDTALAMVNAE
INRQAAMVAYIDDFWLMMWVTLLSVPLVLLLRPPRAGAPKASAADMGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=493, Percent_Identity=32.0486815415822, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788710, Length=494, Percent_Identity=33.1983805668016, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1790195, Length=466, Percent_Identity=30.2575107296137, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788392, Length=403, Percent_Identity=31.2655086848635, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI87081983, Length=447, Percent_Identity=27.9642058165548, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788509, Length=137, Percent_Identity=30.6569343065693, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87082320, Length=178, Percent_Identity=28.0898876404494, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790146, Length=159, Percent_Identity=28.9308176100629, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787065, Length=152, Percent_Identity=29.6052631578947, Blast_Score=63, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=31.3333333333333, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319770, Length=147, Percent_Identity=26.530612244898, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=27.1317829457364, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=246, Percent_Identity=24.7967479674797, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57018; Mature: 56886

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRSLPRRPAAAALPADDMIPLHERVRYRGLLTVAVMGASIMQILDTTIANVAIPHMQS
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGATSETVTWVLTSYILASAIAMPITGWLADRIGRRELFLAAIAGFIVASMACGAAQSL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EQMVAFRFLQGIFAAFIGPLSQSVMLDINPPERHARAMSIWGMGIMIGPILGPVLGGWLT
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ESANWRWVFYVNLPVGLVTLALMWALLPAMRRTSRKFDLFGFSMLALGLAALQLMLDRGA
CCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
HLDWFDSIEIWIELGVATACLWMFFVHLFTARAPLFSRAMLADRNLVTAMGFMIVIGIVM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
FASMALLPPMLQNLFGWPVIDTGLVLAVRGVGILASMWVAGQLLGKVDARWLVGTGLAIA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHH
AYSLWQMSHWSLAMGMQPVIVSGLVQGLGMGLIFIPLNTMAFATIAPQHRTDGSSLLNLL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEHHHHHHHEECCCCCCCHHHHHHHH
RSLGASVGISVVTTLLGINIQTSHQDLAAHVTNSSVALIDPSTADRFGVVGDTALAMVNA
HHHCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
EINRQAAMVAYIDDFWLMMWVTLLSVPLVLLLRPPRAGAPKASAADMGH
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASRSLPRRPAAAALPADDMIPLHERVRYRGLLTVAVMGASIMQILDTTIANVAIPHMQS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGATSETVTWVLTSYILASAIAMPITGWLADRIGRRELFLAAIAGFIVASMACGAAQSL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EQMVAFRFLQGIFAAFIGPLSQSVMLDINPPERHARAMSIWGMGIMIGPILGPVLGGWLT
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ESANWRWVFYVNLPVGLVTLALMWALLPAMRRTSRKFDLFGFSMLALGLAALQLMLDRGA
CCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
HLDWFDSIEIWIELGVATACLWMFFVHLFTARAPLFSRAMLADRNLVTAMGFMIVIGIVM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
FASMALLPPMLQNLFGWPVIDTGLVLAVRGVGILASMWVAGQLLGKVDARWLVGTGLAIA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHH
AYSLWQMSHWSLAMGMQPVIVSGLVQGLGMGLIFIPLNTMAFATIAPQHRTDGSSLLNLL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEHHHHHHHEECCCCCCCHHHHHHHH
RSLGASVGISVVTTLLGINIQTSHQDLAAHVTNSSVALIDPSTADRFGVVGDTALAMVNA
HHHCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
EINRQAAMVAYIDDFWLMMWVTLLSVPLVLLLRPPRAGAPKASAADMGH
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]