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The map label for this gene is greB [H]

Identifier: 103487218

GI number: 103487218

Start: 1824969

End: 1825451

Strand: Direct

Name: greB [H]

Synonym: Sala_1733

Alternate gene names: 103487218

Gene position: 1824969-1825451 (Clockwise)

Preceding gene: 103487217

Following gene: 103487227

Centisome position: 54.56

GC content: 68.32

Gene sequence:

>483_bases
GTGACGGGGGATAAAACCAACATCATCAGCCCGGCGGGCTTTGCCGCGCTGCGCGCCGAATATGACGCGCTGCTCGGCGA
CGAGCGGCCCAAGCTGGTCGAGGTGATCAGCTGGGCCGCGGGCAATGGCGACCGCAGCGAAAATGGCGACTATATCTATG
GCCGCAAACGGCTGCGCGAAATCGACCGGCGCCTCGGCTTCCTCGCGCGCCGCATGAAGGCGGCGCGCGTCGTCGATCCT
GCGCAGCAGCCCGACAAAAGCCGCATCTGGTTCGGCGCGACGGTCGAGCTGGCCGATGACGACGATGCCCGCCGCACCGT
CACGCTGGTCGGCGACGATGAGGCAGAGGCGGGTTCAGGCCGGATCGGCTGGAACAGCCCGCTCGCCCGCGCACTGCGCG
GCGCCGCGGTCGGCGATCTCCGGACGGTGCAGCTTCCCGCGGGGCCGAAATTATGGGAAGTGATCGCGATCCGCTATCCC
TGA

Upstream 100 bases:

>100_bases
GAAAATGCCGTGGTCTACGACACGCTGCGCGACGGAAAGACGCTTCCCAGGGCGCCGCTGAGCTTCTATCTCGGCAAGCG
CACACCGGGATAAGGTCCGC

Downstream 100 bases:

>100_bases
GCGTCACTCGCCGATGCCCGCTATCAACTCGCGCTGGTCCTTGCGCAGTTCGGGCAGATCGCGTGCCAGCTTTGCCCAGT
GCGTCGCTTCGCGCGGGTCG

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTGDKTNIISPAGFAALRAEYDALLGDERPKLVEVISWAAGNGDRSENGDYIYGRKRLREIDRRLGFLARRMKAARVVDP
AQQPDKSRIWFGATVELADDDDARRTVTLVGDDEAEAGSGRIGWNSPLARALRGAAVGDLRTVQLPAGPKLWEVIAIRYP

Sequences:

>Translated_160_residues
MTGDKTNIISPAGFAALRAEYDALLGDERPKLVEVISWAAGNGDRSENGDYIYGRKRLREIDRRLGFLARRMKAARVVDP
AQQPDKSRIWFGATVELADDDDARRTVTLVGDDEAEAGSGRIGWNSPLARALRGAAVGDLRTVQLPAGPKLWEVIAIRYP
>Mature_159_residues
TGDKTNIISPAGFAALRAEYDALLGDERPKLVEVISWAAGNGDRSENGDYIYGRKRLREIDRRLGFLARRMKAARVVDPA
QQPDKSRIWFGATVELADDDDARRTVTLVGDDEAEAGSGRIGWNSPLARALRGAAVGDLRTVQLPAGPKLWEVIAIRYP

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=41.9354838709677, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI87082225, Length=148, Percent_Identity=34.4594594594595, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17562; Mature: 17431

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGDKTNIISPAGFAALRAEYDALLGDERPKLVEVISWAAGNGDRSENGDYIYGRKRLRE
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHH
IDRRLGFLARRMKAARVVDPAQQPDKSRIWFGATVELADDDDARRTVTLVGDDEAEAGSG
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEECCCCCCCCC
RIGWNSPLARALRGAAVGDLRTVQLPAGPKLWEVIAIRYP
CCCCCCHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECC
>Mature Secondary Structure 
TGDKTNIISPAGFAALRAEYDALLGDERPKLVEVISWAAGNGDRSENGDYIYGRKRLRE
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHH
IDRRLGFLARRMKAARVVDPAQQPDKSRIWFGATVELADDDDARRTVTLVGDDEAEAGSG
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEECCCCCCCCC
RIGWNSPLARALRGAAVGDLRTVQLPAGPKLWEVIAIRYP
CCCCCCHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]