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The map label for this gene is slt [H]

Identifier: 103487217

GI number: 103487217

Start: 1822922

End: 1824961

Strand: Direct

Name: slt [H]

Synonym: Sala_1732

Alternate gene names: 103487217

Gene position: 1822922-1824961 (Clockwise)

Preceding gene: 103487203

Following gene: 103487218

Centisome position: 54.49

GC content: 69.02

Gene sequence:

>2040_bases
ATGGCCGCCATGTTCTCGATGCCCTCCCTGATGCCCTCCCTTTCGCGCCTTGCGCTGACCCTGGCGCTCGCCCTGCCCAC
CGCCGCCGCTGCCGGCGAACTCACCCCCGAACAAATGGCCTGGTACCGCGCCCAGATGGGTCTGGCGTCGGCATCGGGCC
CGCCCCCCGCACCCAGTTCGATCGGCGAAGCCGTGATGGAGTGGCGGCGCCTCACCGCGAACAGCGGCGCCTCCTTCGAT
CAGCTGTCGCGCTTCCTGATGGCGAACAAGGGCTGGCCCGACACCGACAAGCTGCGCCTGCGGGCCGAAAAGGCGATTGC
TCTCGACAGTTTCGATCCCGCGCGCACCCTCGCCTATTTCGCCGCCTATCCGCCACAGACGGCCAGCGGCCATCTTCGCA
TGGCAATGGCGCTGAACGCATCGGGGCGGCGACAGGAAGCCCATGCCGCGGTGCGCCGCGCGTGGACCAGCGGGCCGCTC
GACGATTATGAAACGAGCCGTGTGCTCGGCATGTTCCCCGGCGCGCTGACGCTCGCCGACCATGATGCGCGCATGGACAA
GCTGCTCTGGATGGGCGCAACCGCGGCGGCCAGCCGTCAGATCGGCTATACCTCGCCCGAAAAGCGCGCCGTCTTCGCCG
CGCGCCTCGCGATGCGCAGCGGCGCGGTCGATGCCGCGCTTCAGGCGTCGGCCGTCGAAAGCGCCAATCCGTCGATCGTC
CGCACCGACCCCGGCTATATCACCGACAAGGCGACGTGGCTCCGCGCGTCGGGTCGCGTAGGTGAAGCGCGCGCGCTGCT
CGCCGCGCCGCGATCGCTGGCGCAGGCGCCGACCGATGCCGAGGAGTGGCTGGAAACCCTGCTCACCAACGCACGGCTGG
CCGAGAGCAGCGGCGACAAGCTGAGCGCCTTCAACATCGCGCGCCAGCTCGACGACGCATTCCCCCCCGGCACCGTGATC
CGCGAAACGCCGCTCGGGGTGCGCGACGATTATACCTCGCTCGCCTGGCTGGCGGGCCAGCTTGCCTTCAGGGATCTCGG
TCGCCCGGCCGACGCCGAGAAGCTCTACCGCGCCTATGGCGAAGCGGCACGCTCGGCGCAGACGCGCACCAAGGGCTTCT
ACTGGGCCGGCCGCGCCGCACTCGCGGCAGGCGACCGCGACGCGGCAAACGCCCATTTCGCTGATGCGGCGCAGCATTAT
GACCAATTCTATGGCCAGCTGGCGCTCGAACGGCTGGGCCGTCCGCAACCCCGACCGGCGCCCAATCCGACGATCGAGGT
CAGCGCCGACGAACAGCGCGCGTTCGAGGACGACCGGCTGGTCCGCGCCGCGCGCGCGCTGGGCGAAATCGGCGCCTGGC
GCGAGCAGTCGCTGTTCCTCCGCGCGCTCGCGCAAAAGGCCAGCACGCCCGCCGACCATGTGCTCGCGGGCAAGCTCGCC
GCGCAGATCGGCCGCCCCGACCTCGGCGTGATGATCGGCCGCAGCGCGCAGGCGAACAGCCTCGATGCGGTCGAGGTTTC
GGGCTTCCCCACCGTGCGTGTCCCCGCCGGGCACGAGGGCAACTGGACCTTCATCCACGCGATCACGCGGCAGGAAAGCC
AGTTCGACCGCGCCGCGATCAGCCACGCCGGGGCGCGCGGCATGATGCAGCTGATGCCCGGCACCGCGCGCGAGGTCGCG
GGCAAGCTGGGGCTGAGCTACGACGGCGGCGCGCTCACCACCGACACCAACTACAATATGACGCTCGGTTCGACCTATTT
TCAGCAGATGCTGCGCTATTATGGCGGCAGCTATCCGCTCGCGGTCGCGGCCTATAACGCCGGGCCGGGCAATGTGAACA
AATGGCTGCGCGCCAATGGCGACCCGCGCACCGGCGCCATCGACATTCTCGACTGGATCGAGGCGATCCCGATCTTCGAG
ACCAGAAATTATGTCCAGCGCGTGCTCGAAAATGCCGTGGTCTACGACACGCTGCGCGACGGAAAGACGCTTCCCAGGGC
GCCGCTGAGCTTCTATCTCGGCAAGCGCACACCGGGATAA

Upstream 100 bases:

>100_bases
GCTCCGCTCCTTTACAAGGACATGGCCCGACCGATAGAATCCGGCCCGCGCGCCGCTATTGAACGTCTATTCAGCGCCCC
GCAAGGAGTTTGGCATTACG

Downstream 100 bases:

>100_bases
GGTCCGCGTGACGGGGGATAAAACCAACATCATCAGCCCGGCGGGCTTTGCCGCGCTGCGCGCCGAATATGACGCGCTGC
TCGGCGACGAGCGGCCCAAG

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MAAMFSMPSLMPSLSRLALTLALALPTAAAAGELTPEQMAWYRAQMGLASASGPPPAPSSIGEAVMEWRRLTANSGASFD
QLSRFLMANKGWPDTDKLRLRAEKAIALDSFDPARTLAYFAAYPPQTASGHLRMAMALNASGRRQEAHAAVRRAWTSGPL
DDYETSRVLGMFPGALTLADHDARMDKLLWMGATAAASRQIGYTSPEKRAVFAARLAMRSGAVDAALQASAVESANPSIV
RTDPGYITDKATWLRASGRVGEARALLAAPRSLAQAPTDAEEWLETLLTNARLAESSGDKLSAFNIARQLDDAFPPGTVI
RETPLGVRDDYTSLAWLAGQLAFRDLGRPADAEKLYRAYGEAARSAQTRTKGFYWAGRAALAAGDRDAANAHFADAAQHY
DQFYGQLALERLGRPQPRPAPNPTIEVSADEQRAFEDDRLVRAARALGEIGAWREQSLFLRALAQKASTPADHVLAGKLA
AQIGRPDLGVMIGRSAQANSLDAVEVSGFPTVRVPAGHEGNWTFIHAITRQESQFDRAAISHAGARGMMQLMPGTAREVA
GKLGLSYDGGALTTDTNYNMTLGSTYFQQMLRYYGGSYPLAVAAYNAGPGNVNKWLRANGDPRTGAIDILDWIEAIPIFE
TRNYVQRVLENAVVYDTLRDGKTLPRAPLSFYLGKRTPG

Sequences:

>Translated_679_residues
MAAMFSMPSLMPSLSRLALTLALALPTAAAAGELTPEQMAWYRAQMGLASASGPPPAPSSIGEAVMEWRRLTANSGASFD
QLSRFLMANKGWPDTDKLRLRAEKAIALDSFDPARTLAYFAAYPPQTASGHLRMAMALNASGRRQEAHAAVRRAWTSGPL
DDYETSRVLGMFPGALTLADHDARMDKLLWMGATAAASRQIGYTSPEKRAVFAARLAMRSGAVDAALQASAVESANPSIV
RTDPGYITDKATWLRASGRVGEARALLAAPRSLAQAPTDAEEWLETLLTNARLAESSGDKLSAFNIARQLDDAFPPGTVI
RETPLGVRDDYTSLAWLAGQLAFRDLGRPADAEKLYRAYGEAARSAQTRTKGFYWAGRAALAAGDRDAANAHFADAAQHY
DQFYGQLALERLGRPQPRPAPNPTIEVSADEQRAFEDDRLVRAARALGEIGAWREQSLFLRALAQKASTPADHVLAGKLA
AQIGRPDLGVMIGRSAQANSLDAVEVSGFPTVRVPAGHEGNWTFIHAITRQESQFDRAAISHAGARGMMQLMPGTAREVA
GKLGLSYDGGALTTDTNYNMTLGSTYFQQMLRYYGGSYPLAVAAYNAGPGNVNKWLRANGDPRTGAIDILDWIEAIPIFE
TRNYVQRVLENAVVYDTLRDGKTLPRAPLSFYLGKRTPG
>Mature_678_residues
AAMFSMPSLMPSLSRLALTLALALPTAAAAGELTPEQMAWYRAQMGLASASGPPPAPSSIGEAVMEWRRLTANSGASFDQ
LSRFLMANKGWPDTDKLRLRAEKAIALDSFDPARTLAYFAAYPPQTASGHLRMAMALNASGRRQEAHAAVRRAWTSGPLD
DYETSRVLGMFPGALTLADHDARMDKLLWMGATAAASRQIGYTSPEKRAVFAARLAMRSGAVDAALQASAVESANPSIVR
TDPGYITDKATWLRASGRVGEARALLAAPRSLAQAPTDAEEWLETLLTNARLAESSGDKLSAFNIARQLDDAFPPGTVIR
ETPLGVRDDYTSLAWLAGQLAFRDLGRPADAEKLYRAYGEAARSAQTRTKGFYWAGRAALAAGDRDAANAHFADAAQHYD
QFYGQLALERLGRPQPRPAPNPTIEVSADEQRAFEDDRLVRAARALGEIGAWREQSLFLRALAQKASTPADHVLAGKLAA
QIGRPDLGVMIGRSAQANSLDAVEVSGFPTVRVPAGHEGNWTFIHAITRQESQFDRAAISHAGARGMMQLMPGTAREVAG
KLGLSYDGGALTTDTNYNMTLGSTYFQQMLRYYGGSYPLAVAAYNAGPGNVNKWLRANGDPRTGAIDILDWIEAIPIFET
RNYVQRVLENAVVYDTLRDGKTLPRAPLSFYLGKRTPG

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=140, Percent_Identity=39.2857142857143, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73308; Mature: 73176

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAMFSMPSLMPSLSRLALTLALALPTAAAAGELTPEQMAWYRAQMGLASASGPPPAPSS
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHH
IGEAVMEWRRLTANSGASFDQLSRFLMANKGWPDTDKLRLRAEKAIALDSFDPARTLAYF
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHH
AAYPPQTASGHLRMAMALNASGRRQEAHAAVRRAWTSGPLDDYETSRVLGMFPGALTLAD
HCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHCHHHCCCCCEEEECC
HDARMDKLLWMGATAAASRQIGYTSPEKRAVFAARLAMRSGAVDAALQASAVESANPSIV
CHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEE
RTDPGYITDKATWLRASGRVGEARALLAAPRSLAQAPTDAEEWLETLLTNARLAESSGDK
ECCCCEECCCCCEEECCCCCCHHHHHHHCCHHHHCCCCCHHHHHHHHHHCCHHHCCCCCC
LSAFNIARQLDDAFPPGTVIRETPLGVRDDYTSLAWLAGQLAFRDLGRPADAEKLYRAYG
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
EAARSAQTRTKGFYWAGRAALAAGDRDAANAHFADAAQHYDQFYGQLALERLGRPQPRPA
HHHHHHHHHHCCEEEECCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PNPTIEVSADEQRAFEDDRLVRAARALGEIGAWREQSLFLRALAQKASTPADHVLAGKLA
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AQIGRPDLGVMIGRSAQANSLDAVEVSGFPTVRVPAGHEGNWTFIHAITRQESQFDRAAI
HHHCCCCCCEEECCCCCCCCCCEEEECCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHH
SHAGARGMMQLMPGTAREVAGKLGLSYDGGALTTDTNYNMTLGSTYFQQMLRYYGGSYPL
HHHCHHHHHHHCCCCHHHHHHHCCCCCCCCEEEECCCCCEEECHHHHHHHHHHHCCCCCE
AVAAYNAGPGNVNKWLRANGDPRTGAIDILDWIEAIPIFETRNYVQRVLENAVVYDTLRD
EEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
GKTLPRAPLSFYLGKRTPG
CCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
AAMFSMPSLMPSLSRLALTLALALPTAAAAGELTPEQMAWYRAQMGLASASGPPPAPSS
CCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHH
IGEAVMEWRRLTANSGASFDQLSRFLMANKGWPDTDKLRLRAEKAIALDSFDPARTLAYF
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHH
AAYPPQTASGHLRMAMALNASGRRQEAHAAVRRAWTSGPLDDYETSRVLGMFPGALTLAD
HCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHCHHHCCCCCEEEECC
HDARMDKLLWMGATAAASRQIGYTSPEKRAVFAARLAMRSGAVDAALQASAVESANPSIV
CHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEE
RTDPGYITDKATWLRASGRVGEARALLAAPRSLAQAPTDAEEWLETLLTNARLAESSGDK
ECCCCEECCCCCEEECCCCCCHHHHHHHCCHHHHCCCCCHHHHHHHHHHCCHHHCCCCCC
LSAFNIARQLDDAFPPGTVIRETPLGVRDDYTSLAWLAGQLAFRDLGRPADAEKLYRAYG
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
EAARSAQTRTKGFYWAGRAALAAGDRDAANAHFADAAQHYDQFYGQLALERLGRPQPRPA
HHHHHHHHHHCCEEEECCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PNPTIEVSADEQRAFEDDRLVRAARALGEIGAWREQSLFLRALAQKASTPADHVLAGKLA
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AQIGRPDLGVMIGRSAQANSLDAVEVSGFPTVRVPAGHEGNWTFIHAITRQESQFDRAAI
HHHCCCCCCEEECCCCCCCCCCEEEECCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHH
SHAGARGMMQLMPGTAREVAGKLGLSYDGGALTTDTNYNMTLGSTYFQQMLRYYGGSYPL
HHHCHHHHHHHCCCCHHHHHHHCCCCCCCCEEEECCCCCEEECHHHHHHHHHHHCCCCCE
AVAAYNAGPGNVNKWLRANGDPRTGAIDILDWIEAIPIFETRNYVQRVLENAVVYDTLRD
EEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
GKTLPRAPLSFYLGKRTPG
CCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]