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The map label for this gene is ald [H]

Identifier: 103486051

GI number: 103486051

Start: 572566

End: 573681

Strand: Direct

Name: ald [H]

Synonym: Sala_0558

Alternate gene names: 103486051

Gene position: 572566-573681 (Clockwise)

Preceding gene: 103486046

Following gene: 103486052

Centisome position: 17.12

GC content: 65.86

Gene sequence:

>1116_bases
ATGATCATCGGCACCCCCAAGGAAATCAAGAATCACGAATATCGCGTCGGGCTGACCCCCGAAAGCGCGCGCGAGCTCGT
CGTCCACGGACACCGCGTGCTGGTCGAAACCGGCGCGGGTGAGGGGATTGGCGCACACGACCGCTACTATGTCGAAGCGG
GCGCCGAGATCGTCCAGAGCGCCGATGATATTTTTGCGGGCGCCGACATGGTCGTGAAGGTCAAGGAGCCGCAGCCGGTC
GAGCGCGCGATGCTGCGCGAGGGACAGATCCTCTATACCTATCTTCACCTTGCCCCCGACCCCGACCAGACGCGCGACCT
GATGAAGTCGGGCGCGGTGTGCATCGCTTATGAAACGGTCACGAGCCCGCACGGCGGCCTGCCGCTCCTGAAGCCGATGA
GCCAGGTTGCGGGGCGCATGTCGATCCAGGCGGGGGCGACCGCGCTTGAAAAGGCGCACGGTGGCCGCGGCGTACTATTG
GGCGGCGTCCCCGGCGTCGCGCCGGGCAAGGTGTGCGTGATCGGCGGCGGGGTCGTCGGCTTCAACGCCGCGCAGATGGC
GGCGGGGCTCGGCGCCGACGTCACCATCCTCGACCGCGATCCCGAAGTGCTCGAGCGCGTCGGCACCTTTTTCGAAGCGC
GGGCGAAGACGCGCTTCTCGAATCGCGCGAACCTTGCCGAATGCGTCGCCGAGGCCGATCTGGTGATCGGCGCCGTGCTG
ATCCCCGGCGCCGAGGCGCCCAAGCTCGTCAACCGCGAGATGCTCGGCACGATGCGCCCCGGCTCGGTGCTCGTCGACGT
CGCGATCGATCAGGGCGGCTGTTTCGAAACCAGCCATGCGACCACCCATGCCGACCCGACCTATGTGATCGACGGCATCG
TCCATTATGCGGTCGCGAACATGCCGGGCGCGGTCGCGCGCACCAGCACCTATGCGCTCAACAACGTCACCCTGCCGCAC
GCGCTGCGCATCGCCGACCTGGGCTGGAAAGAGGCATTGCGCCGCGATCCGCATCTTTTGCAGGGTTTGAACGTATGGAA
TGGTAAGGTGACGTTCGAAGCCGTAGCGGAAGCGATCGGAACCGATTATGTGCCTGTCGAACAGGCACTCGACTGA

Upstream 100 bases:

>100_bases
GGATCATATGCAAGCACCTCGCGGCGCTTTGCTGCTATTCTCGTCTGATCGCTCCGCGTGCCGGCCGGGCGCGCTGCGGG
CGAGGATATTGGAGAGCAAG

Downstream 100 bases:

>100_bases
CGACAAGGACCATGAAGCCAGATGACCGACCAAGCCCCGCCGCCCGCCGACGACGAAGCCCAGGAAAATGAAGTGCTGCG
CGCCGCGCGCGAGCTGCAAC

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MIIGTPKEIKNHEYRVGLTPESARELVVHGHRVLVETGAGEGIGAHDRYYVEAGAEIVQSADDIFAGADMVVKVKEPQPV
ERAMLREGQILYTYLHLAPDPDQTRDLMKSGAVCIAYETVTSPHGGLPLLKPMSQVAGRMSIQAGATALEKAHGGRGVLL
GGVPGVAPGKVCVIGGGVVGFNAAQMAAGLGADVTILDRDPEVLERVGTFFEARAKTRFSNRANLAECVAEADLVIGAVL
IPGAEAPKLVNREMLGTMRPGSVLVDVAIDQGGCFETSHATTHADPTYVIDGIVHYAVANMPGAVARTSTYALNNVTLPH
ALRIADLGWKEALRRDPHLLQGLNVWNGKVTFEAVAEAIGTDYVPVEQALD

Sequences:

>Translated_371_residues
MIIGTPKEIKNHEYRVGLTPESARELVVHGHRVLVETGAGEGIGAHDRYYVEAGAEIVQSADDIFAGADMVVKVKEPQPV
ERAMLREGQILYTYLHLAPDPDQTRDLMKSGAVCIAYETVTSPHGGLPLLKPMSQVAGRMSIQAGATALEKAHGGRGVLL
GGVPGVAPGKVCVIGGGVVGFNAAQMAAGLGADVTILDRDPEVLERVGTFFEARAKTRFSNRANLAECVAEADLVIGAVL
IPGAEAPKLVNREMLGTMRPGSVLVDVAIDQGGCFETSHATTHADPTYVIDGIVHYAVANMPGAVARTSTYALNNVTLPH
ALRIADLGWKEALRRDPHLLQGLNVWNGKVTFEAVAEAIGTDYVPVEQALD
>Mature_371_residues
MIIGTPKEIKNHEYRVGLTPESARELVVHGHRVLVETGAGEGIGAHDRYYVEAGAEIVQSADDIFAGADMVVKVKEPQPV
ERAMLREGQILYTYLHLAPDPDQTRDLMKSGAVCIAYETVTSPHGGLPLLKPMSQVAGRMSIQAGATALEKAHGGRGVLL
GGVPGVAPGKVCVIGGGVVGFNAAQMAAGLGADVTILDRDPEVLERVGTFFEARAKTRFSNRANLAECVAEADLVIGAVL
IPGAEAPKLVNREMLGTMRPGSVLVDVAIDQGGCFETSHATTHADPTYVIDGIVHYAVANMPGAVARTSTYALNNVTLPH
ALRIADLGWKEALRRDPHLLQGLNVWNGKVTFEAVAEAIGTDYVPVEQALD

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=346, Percent_Identity=31.7919075144509, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI122939153, Length=346, Percent_Identity=31.7919075144509, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1787887, Length=346, Percent_Identity=31.5028901734104, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17550456, Length=335, Percent_Identity=29.8507462686567, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 39260; Mature: 39260

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGTPKEIKNHEYRVGLTPESARELVVHGHRVLVETGAGEGIGAHDRYYVEAGAEIVQS
CCCCCCHHHCCCCEEEECCCHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHH
ADDIFAGADMVVKVKEPQPVERAMLREGQILYTYLHLAPDPDQTRDLMKSGAVCIAYETV
HHHHHCCCCEEEEECCCCHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHCCCEEEEEEEE
TSPHGGLPLLKPMSQVAGRMSIQAGATALEKAHGGRGVLLGGVPGVAPGKVCVIGGGVVG
CCCCCCCCHHHHHHHHHHCEEHHHCHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCEEC
FNAAQMAAGLGADVTILDRDPEVLERVGTFFEARAKTRFSNRANLAECVAEADLVIGAVL
CCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEE
IPGAEAPKLVNREMLGTMRPGSVLVDVAIDQGGCFETSHATTHADPTYVIDGIVHYAVAN
ECCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHC
MPGAVARTSTYALNNVTLPHALRIADLGWKEALRRDPHLLQGLNVWNGKVTFEAVAEAIG
CCCCHHHCCEEEECCCCCCHHEEEECCCHHHHHCCCCHHHHCCCCCCCEEEHHHHHHHHC
TDYVPVEQALD
CCCCCHHHHCC
>Mature Secondary Structure
MIIGTPKEIKNHEYRVGLTPESARELVVHGHRVLVETGAGEGIGAHDRYYVEAGAEIVQS
CCCCCCHHHCCCCEEEECCCHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHH
ADDIFAGADMVVKVKEPQPVERAMLREGQILYTYLHLAPDPDQTRDLMKSGAVCIAYETV
HHHHHCCCCEEEEECCCCHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHCCCEEEEEEEE
TSPHGGLPLLKPMSQVAGRMSIQAGATALEKAHGGRGVLLGGVPGVAPGKVCVIGGGVVG
CCCCCCCCHHHHHHHHHHCEEHHHCHHHHHHCCCCCEEEEECCCCCCCCCEEEEECCEEC
FNAAQMAAGLGADVTILDRDPEVLERVGTFFEARAKTRFSNRANLAECVAEADLVIGAVL
CCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEE
IPGAEAPKLVNREMLGTMRPGSVLVDVAIDQGGCFETSHATTHADPTYVIDGIVHYAVAN
ECCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHC
MPGAVARTSTYALNNVTLPHALRIADLGWKEALRRDPHLLQGLNVWNGKVTFEAVAEAIG
CCCCHHHCCEEEECCCCCCHHEEEECCCHHHHHCCCCHHHHCCCCCCCEEEHHHHHHHHC
TDYVPVEQALD
CCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]