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The map label for this gene is truB [H]

Identifier: 103486034

GI number: 103486034

Start: 554649

End: 555605

Strand: Direct

Name: truB [H]

Synonym: Sala_0541

Alternate gene names: 103486034

Gene position: 554649-555605 (Clockwise)

Preceding gene: 103486033

Following gene: 103486035

Centisome position: 16.58

GC content: 65.62

Gene sequence:

>957_bases
ATGAACGGCTGGATCATCCTCGACAAACCCGTGGGGCTGGGCTCGACGCAGGCGGTCGGCGCGGTCAAGCGCGTGTGCCG
CGAGGCGGGGCTGGGCAAGGCCAAGGTCGGCCACGGCGGGACGCTCGACCCGCTGGCGTCGGGCGTTCTGCCGATCGCGC
TCGGCGAGGCGACAAAGCTCTGCGGGCGGATGCTCGATGCCAGCAAGACCTATGATTTTACAGTGCGGTTTGGGATGGAA
ACCGCGGGGCTCGATGCCGAGGGTGAGGTGGTGGCGACGAGCGACGTGCGGCCGACGCTGGCGGAGATTGATGCGGTGTT
GCCGCGCTTTACCGGACCGATCGAGCAGGTGCCGCCGGCTTACTCCGCAATAAAGATCGATGGCGAGCGGGCGTATGATC
GCGCGCGCAAGGGTGAGGCGGTTGAGATGAAGGCGCGGGGGGTGACCGTCTATTCCCTTCGTCATGCCGGACTTGATCCG
GCATCTATCGCCGCGGGCGCCGAATGGACCCCGGATCAAGTCCGGGGTGACGAGGGCGGGGAGGATCGGCTTGAATCCAT
CACACTTACCGCCCATGTTTCCAAGGGCACATATATCCGCTCGCTCGCCCGCGACATCGCGCGCGCGGTCGGCAGCGTCG
GCCATGTCACCATGCTCCGCCGTACGAAAGCCGGGCCGTTCACGCTCGAACGCGCGATTTCGCTGGACAAATTGAACGCT
TTCGGCCAAGGCGCCGCGCAATCAGAGATTATTCTGCCGCTCGAGGCAGGGCTGGTCGACATCCCGGCTCTTGACCTTTC
CCCGGAAGCGGCAGGGGCGATCCGCCAGGGTCGCGTCTGGACCGGGGTTAGCACGCACGACGGGCTCTATTGGGGACGCG
ACGCCGAGAACCGGCCCGTTGCCTTGATCGAGGCTTTGGCGGGAACGCTGAAGGTAGTGCGGGGCTTCAACCTGTAA

Upstream 100 bases:

>100_bases
CGCGCCGCCACCGTCGAAGCGGGACAAGTCGGAGGACATCATGGGCGAAAACCCGGCGGCAAATTGACAAGCGCCGCCCG
GCGCGCCTAGCGCCGCGGCG

Downstream 100 bases:

>100_bases
GTCACGATGTTCGAGGAAAAGAAATATGTCGATTACCGCCGAGCGCAAAGCCGAAGTCATCAAGGACAATGCCCGCGACA
AGGGCGATACCGGTTCGCCG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNGWIILDKPVGLGSTQAVGAVKRVCREAGLGKAKVGHGGTLDPLASGVLPIALGEATKLCGRMLDASKTYDFTVRFGME
TAGLDAEGEVVATSDVRPTLAEIDAVLPRFTGPIEQVPPAYSAIKIDGERAYDRARKGEAVEMKARGVTVYSLRHAGLDP
ASIAAGAEWTPDQVRGDEGGEDRLESITLTAHVSKGTYIRSLARDIARAVGSVGHVTMLRRTKAGPFTLERAISLDKLNA
FGQGAAQSEIILPLEAGLVDIPALDLSPEAAGAIRQGRVWTGVSTHDGLYWGRDAENRPVALIEALAGTLKVVRGFNL

Sequences:

>Translated_318_residues
MNGWIILDKPVGLGSTQAVGAVKRVCREAGLGKAKVGHGGTLDPLASGVLPIALGEATKLCGRMLDASKTYDFTVRFGME
TAGLDAEGEVVATSDVRPTLAEIDAVLPRFTGPIEQVPPAYSAIKIDGERAYDRARKGEAVEMKARGVTVYSLRHAGLDP
ASIAAGAEWTPDQVRGDEGGEDRLESITLTAHVSKGTYIRSLARDIARAVGSVGHVTMLRRTKAGPFTLERAISLDKLNA
FGQGAAQSEIILPLEAGLVDIPALDLSPEAAGAIRQGRVWTGVSTHDGLYWGRDAENRPVALIEALAGTLKVVRGFNL
>Mature_318_residues
MNGWIILDKPVGLGSTQAVGAVKRVCREAGLGKAKVGHGGTLDPLASGVLPIALGEATKLCGRMLDASKTYDFTVRFGME
TAGLDAEGEVVATSDVRPTLAEIDAVLPRFTGPIEQVPPAYSAIKIDGERAYDRARKGEAVEMKARGVTVYSLRHAGLDP
ASIAAGAEWTPDQVRGDEGGEDRLESITLTAHVSKGTYIRSLARDIARAVGSVGHVTMLRRTKAGPFTLERAISLDKLNA
FGQGAAQSEIILPLEAGLVDIPALDLSPEAAGAIRQGRVWTGVSTHDGLYWGRDAENRPVALIEALAGTLKVVRGFNL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=274, Percent_Identity=34.6715328467153, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI2367200, Length=310, Percent_Identity=33.8709677419355, Blast_Score=164, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6324037, Length=192, Percent_Identity=32.2916666666667, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33530; Mature: 33530

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGWIILDKPVGLGSTQAVGAVKRVCREAGLGKAKVGHGGTLDPLASGVLPIALGEATKL
CCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCEEEEECHHHHH
CGRMLDASKTYDFTVRFGMETAGLDAEGEVVATSDVRPTLAEIDAVLPRFTGPIEQVPPA
HHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHCCCC
YSAIKIDGERAYDRARKGEAVEMKARGVTVYSLRHAGLDPASIAAGAEWTPDQVRGDEGG
CEEEEECCHHHHHHHHCCCEEEEEECCEEEEEEECCCCCHHHHCCCCCCCHHHCCCCCCC
EDRLESITLTAHVSKGTYIRSLARDIARAVGSVGHVTMLRRTKAGPFTLERAISLDKLNA
HHHHHEEEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHH
FGQGAAQSEIILPLEAGLVDIPALDLSPEAAGAIRQGRVWTGVSTHDGLYWGRDAENRPV
HCCCCCCCEEEEEECCCEEECCCCCCCCHHHHHHHCCEEEECCCCCCCEEECCCCCCCCH
ALIEALAGTLKVVRGFNL
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNGWIILDKPVGLGSTQAVGAVKRVCREAGLGKAKVGHGGTLDPLASGVLPIALGEATKL
CCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCEEEEECHHHHH
CGRMLDASKTYDFTVRFGMETAGLDAEGEVVATSDVRPTLAEIDAVLPRFTGPIEQVPPA
HHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHCCCC
YSAIKIDGERAYDRARKGEAVEMKARGVTVYSLRHAGLDPASIAAGAEWTPDQVRGDEGG
CEEEEECCHHHHHHHHCCCEEEEEECCEEEEEEECCCCCHHHHCCCCCCCHHHCCCCCCC
EDRLESITLTAHVSKGTYIRSLARDIARAVGSVGHVTMLRRTKAGPFTLERAISLDKLNA
HHHHHEEEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHH
FGQGAAQSEIILPLEAGLVDIPALDLSPEAAGAIRQGRVWTGVSTHDGLYWGRDAENRPV
HCCCCCCCEEEEEECCCEEECCCCCCCCHHHHHHHCCEEEECCCCCCCEEECCCCCCCCH
ALIEALAGTLKVVRGFNL
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA