| Definition | Sphingopyxis alaskensis RB2256, complete genome. |
|---|---|
| Accession | NC_008048 |
| Length | 3,345,170 |
Click here to switch to the map view.
The map label for this gene is tgs2 [H]
Identifier: 103486026
GI number: 103486026
Start: 548107
End: 549528
Strand: Reverse
Name: tgs2 [H]
Synonym: Sala_0533
Alternate gene names: 103486026
Gene position: 549528-548107 (Counterclockwise)
Preceding gene: 103486027
Following gene: 103486024
Centisome position: 16.43
GC content: 63.57
Gene sequence:
>1422_bases ATGCGGTTGATGCCGGTGACCGATGCGATGTTCCTGTGGGGGGAAAGTCGCGAGATGCCGATGCACATCGGCGGCATCAA CCTCTACACGCTGCCCGACGGGGTCGACGAGACCGAGTGGCTGAACGAACAGCTCACGCTGCTCCGCCAGTCGGAGGGGT TGCGGCGCCCGTTCAGCGAGGTGCTCAAGCTCACCCCCTTCGGTCAATATGGCCCGATCCGCCTTGTGCCCGACCGCGAC ATCGACATGCACTATCATGTCCGCGCCGCCGCGCTGCCCAAGCCGGGGCGCTACCGCGAGATGTTCGAACTCGCCTCGCG GCTCCATTCGGGCCTTCTCGACCGCACCCGCCCCTTGTGGGAAATCACGCTCATCTCCGGGCTGCCCAACCGGCAGATCG CGACATTCAAGAAGGTCCATCACGCGCTTATGGATGGCGCAGCGAGCATCCATTTCTATAATTCGATGCTCTCGCCCGAT CCCGACGAACGGCGCACCGCCTCGCCGCTGTCGGTCGAGGCCTATGAGGCGTACAAGCGCAGCTTCCCCGCCCCCAAAAA ACCGCCGCGCCCCGACCTCACCGACATCAAGGCGATCGGCGATTTCCTGAAGGAACAGTGGGGCAACAGCGTCGGGGTGA CGAAGGCGATGAACCAGTATCTCGCGGCGATGTTCGGCATCGGCGGCGACGGGCTGGCCACGCCCTTTGCGGGCGTGCCG CGCACCAGCTTCAACCGCAACATCACCGGCGCGCGGCGCTTCGTCGCGCAAAGCTGGTCGCTGGAACGGGTCAGGGCGAT GGGCAAGGCTTATGACGGGACGATCAACGACGCGGTGCTCGGCATGTGCGCGGGCGCGATGCGCAAATATCTGCAATCGC TGAACGAACTGCCCGACAAGCCCTTGAAAGCCATGGCGCCGGTGTCGATCCGGCCCAAGGACGACATCGATTCGGGCAAT TCGGTCGCGTCGGTGACCGCGAACCTTGCGACGCATATCGACGATCCGGCGACGCGCATGGCGATGATCCAGGAATCGAT GAACTCGGCGAAGGCGCAGCTTCGCGCGATGACCGCGCAGCAAATACGGCTCTATACCGCGATCACCAGCTTCCCGATGA TGCTCACCGCGCTCACGCGCACCGCCGACAAGTTTCCGGCCTATTCGGTGACCATTTCCAACGTCCCCGGCCCGCGCGAG CAGATGTACTGGAACGGCGCGCGACTCGACGGCATGTATCCCGCAAGCATCCCGGTCGACGGCATGGCGATGAACATCAC GCAGGTGTCGAACTTCAGGAATATCGACTTCGGCATCACCGCCTGCCGCCGCAGCGTCCCGCACGCGCAGCGGATGATCG ACTATCTGGAAGACTCTCTGGTCGAACTCGAAACGGCAGCAGGCATCAAGAAGGCGAAATAG
Upstream 100 bases:
>100_bases ACGCCGCCGCCTGACGAGCCTCCCTCCGCCCCCGGCCTTGACTCACCCACCTCCGCTGCCATGCTGCACGGCCAAGGCGC AGCATAGGGGGAGAGCTGCC
Downstream 100 bases:
>100_bases CCCCGCCAACCCGCATCAGAAATCCACCTGCGTCCGAAGGCCGAAGGTGTCCGCCGTATAATCGCGGTCCCCCGTCGCCG TTGCCACACGTGCATCGCCC
Product: hypothetical protein
Products: NA
Alternate protein names: TGS2; Diacylglycerol O-acyltransferase; DGAT; Long-chain-alcohol O-fatty-acyltransferase; Probable triacylglycerol synthase tgs2; Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase; Wax synthase; WS [H]
Number of amino acids: Translated: 473; Mature: 473
Protein sequence:
>473_residues MRLMPVTDAMFLWGESREMPMHIGGINLYTLPDGVDETEWLNEQLTLLRQSEGLRRPFSEVLKLTPFGQYGPIRLVPDRD IDMHYHVRAAALPKPGRYREMFELASRLHSGLLDRTRPLWEITLISGLPNRQIATFKKVHHALMDGAASIHFYNSMLSPD PDERRTASPLSVEAYEAYKRSFPAPKKPPRPDLTDIKAIGDFLKEQWGNSVGVTKAMNQYLAAMFGIGGDGLATPFAGVP RTSFNRNITGARRFVAQSWSLERVRAMGKAYDGTINDAVLGMCAGAMRKYLQSLNELPDKPLKAMAPVSIRPKDDIDSGN SVASVTANLATHIDDPATRMAMIQESMNSAKAQLRAMTAQQIRLYTAITSFPMMLTALTRTADKFPAYSVTISNVPGPRE QMYWNGARLDGMYPASIPVDGMAMNITQVSNFRNIDFGITACRRSVPHAQRMIDYLEDSLVELETAAGIKKAK
Sequences:
>Translated_473_residues MRLMPVTDAMFLWGESREMPMHIGGINLYTLPDGVDETEWLNEQLTLLRQSEGLRRPFSEVLKLTPFGQYGPIRLVPDRD IDMHYHVRAAALPKPGRYREMFELASRLHSGLLDRTRPLWEITLISGLPNRQIATFKKVHHALMDGAASIHFYNSMLSPD PDERRTASPLSVEAYEAYKRSFPAPKKPPRPDLTDIKAIGDFLKEQWGNSVGVTKAMNQYLAAMFGIGGDGLATPFAGVP RTSFNRNITGARRFVAQSWSLERVRAMGKAYDGTINDAVLGMCAGAMRKYLQSLNELPDKPLKAMAPVSIRPKDDIDSGN SVASVTANLATHIDDPATRMAMIQESMNSAKAQLRAMTAQQIRLYTAITSFPMMLTALTRTADKFPAYSVTISNVPGPRE QMYWNGARLDGMYPASIPVDGMAMNITQVSNFRNIDFGITACRRSVPHAQRMIDYLEDSLVELETAAGIKKAK >Mature_473_residues MRLMPVTDAMFLWGESREMPMHIGGINLYTLPDGVDETEWLNEQLTLLRQSEGLRRPFSEVLKLTPFGQYGPIRLVPDRD IDMHYHVRAAALPKPGRYREMFELASRLHSGLLDRTRPLWEITLISGLPNRQIATFKKVHHALMDGAASIHFYNSMLSPD PDERRTASPLSVEAYEAYKRSFPAPKKPPRPDLTDIKAIGDFLKEQWGNSVGVTKAMNQYLAAMFGIGGDGLATPFAGVP RTSFNRNITGARRFVAQSWSLERVRAMGKAYDGTINDAVLGMCAGAMRKYLQSLNELPDKPLKAMAPVSIRPKDDIDSGN SVASVTANLATHIDDPATRMAMIQESMNSAKAQLRAMTAQQIRLYTAITSFPMMLTALTRTADKFPAYSVTISNVPGPRE QMYWNGARLDGMYPASIPVDGMAMNITQVSNFRNIDFGITACRRSVPHAQRMIDYLEDSLVELETAAGIKKAK
Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Also functions as a wax synthase, incorporating palmityl alcohol into wax esters in the presence of palmitoyl-CoA [H
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the long-chain O-acyltransferase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014292 - InterPro: IPR004255 [H]
Pfam domain/function: PF03007 UPF0089 [H]
EC number: =2.3.1.20; =2.3.1.75 [H]
Molecular weight: Translated: 52661; Mature: 52661
Theoretical pI: Translated: 9.53; Mature: 9.53
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 5.5 %Met (Translated Protein) 5.9 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 5.5 %Met (Mature Protein) 5.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRLMPVTDAMFLWGESREMPMHIGGINLYTLPDGVDETEWLNEQLTLLRQSEGLRRPFSE CCCCCCCHHHHEECCCCCCCEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCHHCCHHHH VLKLTPFGQYGPIRLVPDRDIDMHYHVRAAALPKPGRYREMFELASRLHSGLLDRTRPLW HHHCCCCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCE EITLISGLPNRQIATFKKVHHALMDGAASIHFYNSMLSPDPDERRTASPLSVEAYEAYKR EEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHH SFPAPKKPPRPDLTDIKAIGDFLKEQWGNSVGVTKAMNQYLAAMFGIGGDGLATPFAGVP CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC RTSFNRNITGARRFVAQSWSLERVRAMGKAYDGTINDAVLGMCAGAMRKYLQSLNELPDK CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC PLKAMAPVSIRPKDDIDSGNSVASVTANLATHIDDPATRMAMIQESMNSAKAQLRAMTAQ CHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH QIRLYTAITSFPMMLTALTRTADKFPAYSVTISNVPGPREQMYWNGARLDGMYPASIPVD HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHEECCCEECCCCCCCCCCC GMAMNITQVSNFRNIDFGITACRRSVPHAQRMIDYLEDSLVELETAAGIKKAK CEEEEHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC >Mature Secondary Structure MRLMPVTDAMFLWGESREMPMHIGGINLYTLPDGVDETEWLNEQLTLLRQSEGLRRPFSE CCCCCCCHHHHEECCCCCCCEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCHHCCHHHH VLKLTPFGQYGPIRLVPDRDIDMHYHVRAAALPKPGRYREMFELASRLHSGLLDRTRPLW HHHCCCCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCE EITLISGLPNRQIATFKKVHHALMDGAASIHFYNSMLSPDPDERRTASPLSVEAYEAYKR EEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHH SFPAPKKPPRPDLTDIKAIGDFLKEQWGNSVGVTKAMNQYLAAMFGIGGDGLATPFAGVP CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC RTSFNRNITGARRFVAQSWSLERVRAMGKAYDGTINDAVLGMCAGAMRKYLQSLNELPDK CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC PLKAMAPVSIRPKDDIDSGNSVASVTANLATHIDDPATRMAMIQESMNSAKAQLRAMTAQ CHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH QIRLYTAITSFPMMLTALTRTADKFPAYSVTISNVPGPREQMYWNGARLDGMYPASIPVD HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHEECCCEECCCCCCCCCCC GMAMNITQVSNFRNIDFGITACRRSVPHAQRMIDYLEDSLVELETAAGIKKAK CEEEEHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9634230; 12218036 [H]