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The map label for this gene is pdhB [H]

Identifier: 103486019

GI number: 103486019

Start: 541057

End: 542457

Strand: Direct

Name: pdhB [H]

Synonym: Sala_0526

Alternate gene names: 103486019

Gene position: 541057-542457 (Clockwise)

Preceding gene: 103486018

Following gene: 103486020

Centisome position: 16.17

GC content: 66.24

Gene sequence:

>1401_bases
ATGGCCATCGAACTGAAGATGCCGGCGCTGTCGCCGACGATGGAAGAGGGCACGCTCGCCAAGTGGCTCGTCAAGGAAGG
CGACATTGTGAAGTCGGGCGACATCCTCGCCGAAATCGAAACCGACAAGGCAACGATGGAGTTTGAAGCGGTCGACGAAG
GGACGATCGGCCAGATCCTCGTTCCCGAAGGCACCGACAATGTGAAGGTCGGCACCGTGATCGCGACGATTCAGGGCGAA
GGGGAGGAACAAAACGTCGCCCCCGCGCAGGCGGGGGCCGCAAGCAACAGTGAGCCCACGCCAGCGGCCCCCGCCCCCGC
GGGGGCGACGGATGAGCCTGCACCCGCTCCCGCTCCCGTTGCGGACAGGCCCGCCGCGACCGAACGCGCTTCCGACCCCG
CGATCCCCGAAGGCACCGCGATGGTCAAGCTCACCGTCCGCGAAGCGCTGCGCGACGCGATGGCCGAGGAAATGCGAAGG
GACGACCGCGTCTTTGTGATGGGCGAGGAAGTCGCCGAATATCAGGGCGCGTACAAGGTCACGCAGGGGCTGCTCCAGGA
GTTTGGCGCGCGGCGCGTGGTCGACACGCCGATCACCGAATATGGCTTTGCCGGCCTGGGCGCAGGCGCGGCGATGGGCG
GCCTCAGGCCCGTCATCGAGTTCATGACCTTCAACTTTGCGATGCAGGCGATCGACCACATCATCAATTCGGCGGCGAAG
ACCAACTATATGTCGGGCGGGCAGATGCGCTGCCCGATCGTTTTCCGCGGCCCCAACGGTGCGGCGGCGCGCGTCGGCGC
GCAGCACAGCCAGAATTACGGGCCCTGGTATGCCAGCGTTCCCGGCCTGATCGTCATCGCGCCCTATGACGCCGCCGACG
CCAAGGGGCTGCTGAAGGCGGCGATCCGCACCGAAGACCCGGTCGTCTTCCTCGAAAACGAGCTGCTCTATGGCCGCAGC
TTCGAGGTTCCCGATGTCGATGATTTCGTGCTGCCGATCGGCAAGGCGCGCGTGATGCGCGAAGGGCGCGATGTCACCGT
CGTCAGCTATTCGATCGGCGTCGGCCTGGCGCTCGAGGCGGCCGACAGCCTTGCGGGCGAGGGGATCGACGCCGAGGTGA
TCGACCTGCGTACGCTGCGCCCGCTCGACACCGCGACGGTGCTCGCCAGCCTCAAGAAGACGAACCGCCTTGTCGTCGTC
GAGGAGGGCTGGCCGGTCTGCTCGATCGCCAGCGAAATCGCGATGGTCGCGATGGAGCAGGGCTTCGACGACCTCGACGC
GCCCGTGATGCGCGTGTGCAACGAGGATGTGCCGCTGCCCTATGCGAACAACCTGGAGAAGGCCGCGCTGATCGACACGC
CGCGCGTGGTGACGGCGGTGAAGGCGGTTCTCAATCGTTAA

Upstream 100 bases:

>100_bases
ATCCGTGCGATCGTCGCCGAATCGGCCGACTTTGCCGAGAGCGCGCCCGAACCCGACTTGTCCGAACTCTATACCGACGT
GCTGGTGGAGCAATATTGAG

Downstream 100 bases:

>100_bases
TCCGTCGCCCCCGCGAAGGCGGGGGCCGTCATCGGCCTTGCCCTTCCATGCCGAGTAAACCGACACCGGCCCCCGCCTTC
GCGGGGGCGACGGCTGAACC

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAIELKMPALSPTMEEGTLAKWLVKEGDIVKSGDILAEIETDKATMEFEAVDEGTIGQILVPEGTDNVKVGTVIATIQGE
GEEQNVAPAQAGAASNSEPTPAAPAPAGATDEPAPAPAPVADRPAATERASDPAIPEGTAMVKLTVREALRDAMAEEMRR
DDRVFVMGEEVAEYQGAYKVTQGLLQEFGARRVVDTPITEYGFAGLGAGAAMGGLRPVIEFMTFNFAMQAIDHIINSAAK
TNYMSGGQMRCPIVFRGPNGAAARVGAQHSQNYGPWYASVPGLIVIAPYDAADAKGLLKAAIRTEDPVVFLENELLYGRS
FEVPDVDDFVLPIGKARVMREGRDVTVVSYSIGVGLALEAADSLAGEGIDAEVIDLRTLRPLDTATVLASLKKTNRLVVV
EEGWPVCSIASEIAMVAMEQGFDDLDAPVMRVCNEDVPLPYANNLEKAALIDTPRVVTAVKAVLNR

Sequences:

>Translated_466_residues
MAIELKMPALSPTMEEGTLAKWLVKEGDIVKSGDILAEIETDKATMEFEAVDEGTIGQILVPEGTDNVKVGTVIATIQGE
GEEQNVAPAQAGAASNSEPTPAAPAPAGATDEPAPAPAPVADRPAATERASDPAIPEGTAMVKLTVREALRDAMAEEMRR
DDRVFVMGEEVAEYQGAYKVTQGLLQEFGARRVVDTPITEYGFAGLGAGAAMGGLRPVIEFMTFNFAMQAIDHIINSAAK
TNYMSGGQMRCPIVFRGPNGAAARVGAQHSQNYGPWYASVPGLIVIAPYDAADAKGLLKAAIRTEDPVVFLENELLYGRS
FEVPDVDDFVLPIGKARVMREGRDVTVVSYSIGVGLALEAADSLAGEGIDAEVIDLRTLRPLDTATVLASLKKTNRLVVV
EEGWPVCSIASEIAMVAMEQGFDDLDAPVMRVCNEDVPLPYANNLEKAALIDTPRVVTAVKAVLNR
>Mature_465_residues
AIELKMPALSPTMEEGTLAKWLVKEGDIVKSGDILAEIETDKATMEFEAVDEGTIGQILVPEGTDNVKVGTVIATIQGEG
EEQNVAPAQAGAASNSEPTPAAPAPAGATDEPAPAPAPVADRPAATERASDPAIPEGTAMVKLTVREALRDAMAEEMRRD
DRVFVMGEEVAEYQGAYKVTQGLLQEFGARRVVDTPITEYGFAGLGAGAAMGGLRPVIEFMTFNFAMQAIDHIINSAAKT
NYMSGGQMRCPIVFRGPNGAAARVGAQHSQNYGPWYASVPGLIVIAPYDAADAKGLLKAAIRTEDPVVFLENELLYGRSF
EVPDVDDFVLPIGKARVMREGRDVTVVSYSIGVGLALEAADSLAGEGIDAEVIDLRTLRPLDTATVLASLKKTNRLVVVE
EGWPVCSIASEIAMVAMEQGFDDLDAPVMRVCNEDVPLPYANNLEKAALIDTPRVVTAVKAVLNR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=330, Percent_Identity=54.8484848484848, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI291084858, Length=330, Percent_Identity=51.2121212121212, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI4557353, Length=337, Percent_Identity=33.2344213649852, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI34101272, Length=337, Percent_Identity=33.2344213649852, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI203098753, Length=81, Percent_Identity=51.8518518518518, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI203098816, Length=81, Percent_Identity=51.8518518518518, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI31711992, Length=80, Percent_Identity=47.5, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI260898739, Length=82, Percent_Identity=46.3414634146341, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI225637463, Length=311, Percent_Identity=28.6173633440514, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI225637459, Length=311, Percent_Identity=28.6173633440514, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI225637461, Length=311, Percent_Identity=28.6173633440514, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=62.5766871165644, Blast_Score=410, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17506935, Length=337, Percent_Identity=35.3115727002967, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=129, Percent_Identity=42.6356589147287, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=59.9388379204893, Blast_Score=408, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324258, Length=139, Percent_Identity=41.0071942446043, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321632, Length=73, Percent_Identity=45.2054794520548, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21358145, Length=327, Percent_Identity=60.8562691131498, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24650940, Length=327, Percent_Identity=60.8562691131498, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI160714832, Length=325, Percent_Identity=32.3076923076923, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI160714828, Length=325, Percent_Identity=32.3076923076923, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24650943, Length=97, Percent_Identity=62.8865979381443, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24650945, Length=97, Percent_Identity=62.8865979381443, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI20129315, Length=75, Percent_Identity=52, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24582497, Length=64, Percent_Identity=50, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 49440; Mature: 49309

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIELKMPALSPTMEEGTLAKWLVKEGDIVKSGDILAEIETDKATMEFEAVDEGTIGQIL
CEEEEECCCCCCCCCCCCHHHHHHCCCCEEECCCEEEEEECCCCEEEEEECCCCCCCEEE
VPEGTDNVKVGTVIATIQGEGEEQNVAPAQAGAASNSEPTPAAPAPAGATDEPAPAPAPV
CCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADRPAATERASDPAIPEGTAMVKLTVREALRDAMAEEMRRDDRVFVMGEEVAEYQGAYKV
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCHHHH
TQGLLQEFGARRVVDTPITEYGFAGLGAGAAMGGLRPVIEFMTFNFAMQAIDHIINSAAK
HHHHHHHHCCCEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNYMSGGQMRCPIVFRGPNGAAARVGAQHSQNYGPWYASVPGLIVIAPYDAADAKGLLKA
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHHHH
AIRTEDPVVFLENELLYGRSFEVPDVDDFVLPIGKARVMREGRDVTVVSYSIGVGLALEA
HHCCCCCEEEEECCEEECCCCCCCCCCHHHHCCCHHHHHHCCCCEEEEEEECCCCEEEHH
ADSLAGEGIDAEVIDLRTLRPLDTATVLASLKKTNRLVVVEEGWPVCSIASEIAMVAMEQ
HHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHC
GFDDLDAPVMRVCNEDVPLPYANNLEKAALIDTPRVVTAVKAVLNR
CCCCCCHHHHHHCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AIELKMPALSPTMEEGTLAKWLVKEGDIVKSGDILAEIETDKATMEFEAVDEGTIGQIL
EEEEECCCCCCCCCCCCHHHHHHCCCCEEECCCEEEEEECCCCEEEEEECCCCCCCEEE
VPEGTDNVKVGTVIATIQGEGEEQNVAPAQAGAASNSEPTPAAPAPAGATDEPAPAPAPV
CCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ADRPAATERASDPAIPEGTAMVKLTVREALRDAMAEEMRRDDRVFVMGEEVAEYQGAYKV
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCHHHH
TQGLLQEFGARRVVDTPITEYGFAGLGAGAAMGGLRPVIEFMTFNFAMQAIDHIINSAAK
HHHHHHHHCCCEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNYMSGGQMRCPIVFRGPNGAAARVGAQHSQNYGPWYASVPGLIVIAPYDAADAKGLLKA
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHHHH
AIRTEDPVVFLENELLYGRSFEVPDVDDFVLPIGKARVMREGRDVTVVSYSIGVGLALEA
HHCCCCCEEEEECCEEECCCCCCCCCCHHHHCCCHHHHHHCCCCEEEEEEECCCCEEEHH
ADSLAGEGIDAEVIDLRTLRPLDTATVLASLKKTNRLVVVEEGWPVCSIASEIAMVAMEQ
HHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHC
GFDDLDAPVMRVCNEDVPLPYANNLEKAALIDTPRVVTAVKAVLNR
CCCCCCHHHHHHCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]