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The map label for this gene is eno

Identifier: 103486016

GI number: 103486016

Start: 538057

End: 539331

Strand: Direct

Name: eno

Synonym: Sala_0523

Alternate gene names: 103486016

Gene position: 538057-539331 (Clockwise)

Preceding gene: 103486010

Following gene: 103486017

Centisome position: 16.08

GC content: 64.94

Gene sequence:

>1275_bases
ATGACCGCCATCATCGACATCCACGGCCGCGAAATTCTCGACAGCCGCGGCAACCCCACCGTCGAAGTCGATGTGCTGCT
GGAGGACGGCAGCTTCGGCCGTGCCGCGGTTCCCTCGGGCGCCTCGACCGGCGCACACGAGGCCGTCGAACTGCGCGACG
GCGACAAGGGGCGCTATCTCGGCAAGGGCGTGACCAAAGCGGTCGCGGCGGTCAACGGCGACATCGCCGAGGCGCTGGTC
GGCCTCGATGCCGAGGACCAGCGCGAGATTGACATGGCAATGATCGACCTCGACGGCACGCCGAACAAGAGCCGCCTTGG
CGCCAATGCGATTTTGGGCGTCAGCCTTGCCGCCGCGAAGGCCGCCGCCGACGCGCGCGGCCTGCCGCTCTATCGCTATG
TCGGCGGCGTGTCGGCGCGCACCTTGCCGGTGCCGATGATGAACATCATCAACGGCGGCGAACATGCCGACAATCCGATC
GACGTGCAGGAGTTCATGATCATGCCCGTCGGCGCCGGCAGCATCGCCGAAGCGGTGCGCTGGGGCAGCGAGATTTTCCA
CACGCTGAAGAAGGGCCTGTCACAGAAGGGCCTCGCGACCGCCGTGGGCGACGAGGGTGGTTTCGCCCCCAACCTTGCCT
CGACCCGCGATGCGCTCGATTTCATCGCCGCCTCGGTCGACCAGGCCGGTTTCAAGCTCGGCACCGACGTCGTGCTCGCG
CTCGATTGCGCCGCGACCGAGTTTTTCAGGAATGGCAAATATGAAATCAGCGGCGAAGGCCTGTCGCTGAGCCCCGAACA
AATGGCCGAATATCTCGCCGCGCTCGTCAAGGATTACCCGATCAAGTCGATCGAGGACGGGATGAGCGAAGATGATTTCG
CGGGCTGGAAGGCACTGACCGACCTGATCGGCGGCACGTGCCAGCTCGTCGGCGACGATCTGTTCGTCACCAACCCGGCG
CGGCTGGAACAGGGGATCAAGGACGGCCTTGCCAATTCGCTGCTTGTCAAAGTCAACCAGATCGGCACGCTGTCCGAAAC
ATTGGATGCGGTCGATATGGCGCACCGCGCGCGCTATTCTGCGGTGATGTCGCACCGCTCGGGCGAGACCGAGGATGCGA
CGATCGCCGACCTTGCCGTCGCGACCAACTGCGGCCAGATCAAGACCGGCAGCCTCGCGCGGTCGGACCGGCTTGCGAAA
TACAACCAGCTGATCCGCATCGAGGAAGAGCTGGGCGATATGGCGCGCTATCCGGGCGCGGCGATTTTCGGCTGA

Upstream 100 bases:

>100_bases
CGCAAAATTCCTATGCGTATTGCGCTCGCACGCGGGCGCCGATAACAGGCAGGCGCCTTATCCATCCGGCCGCATCCGGC
CGTCAGCAGGGACATCATCC

Downstream 100 bases:

>100_bases
CCGCAAAGCCGGGGACAAGTGCGCGCTGGCGCCTTGACGCCGGGAATCGCCTGTGATTCAAGGACGGCGATGACGCAGCG
CCACAAATTACGCAAATCGA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTAIIDIHGREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAVELRDGDKGRYLGKGVTKAVAAVNGDIAEALV
GLDAEDQREIDMAMIDLDGTPNKSRLGANAILGVSLAAAKAAADARGLPLYRYVGGVSARTLPVPMMNIINGGEHADNPI
DVQEFMIMPVGAGSIAEAVRWGSEIFHTLKKGLSQKGLATAVGDEGGFAPNLASTRDALDFIAASVDQAGFKLGTDVVLA
LDCAATEFFRNGKYEISGEGLSLSPEQMAEYLAALVKDYPIKSIEDGMSEDDFAGWKALTDLIGGTCQLVGDDLFVTNPA
RLEQGIKDGLANSLLVKVNQIGTLSETLDAVDMAHRARYSAVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEELGDMARYPGAAIFG

Sequences:

>Translated_424_residues
MTAIIDIHGREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAVELRDGDKGRYLGKGVTKAVAAVNGDIAEALV
GLDAEDQREIDMAMIDLDGTPNKSRLGANAILGVSLAAAKAAADARGLPLYRYVGGVSARTLPVPMMNIINGGEHADNPI
DVQEFMIMPVGAGSIAEAVRWGSEIFHTLKKGLSQKGLATAVGDEGGFAPNLASTRDALDFIAASVDQAGFKLGTDVVLA
LDCAATEFFRNGKYEISGEGLSLSPEQMAEYLAALVKDYPIKSIEDGMSEDDFAGWKALTDLIGGTCQLVGDDLFVTNPA
RLEQGIKDGLANSLLVKVNQIGTLSETLDAVDMAHRARYSAVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEELGDMARYPGAAIFG
>Mature_423_residues
TAIIDIHGREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAVELRDGDKGRYLGKGVTKAVAAVNGDIAEALVG
LDAEDQREIDMAMIDLDGTPNKSRLGANAILGVSLAAAKAAADARGLPLYRYVGGVSARTLPVPMMNIINGGEHADNPID
VQEFMIMPVGAGSIAEAVRWGSEIFHTLKKGLSQKGLATAVGDEGGFAPNLASTRDALDFIAASVDQAGFKLGTDVVLAL
DCAATEFFRNGKYEISGEGLSLSPEQMAEYLAALVKDYPIKSIEDGMSEDDFAGWKALTDLIGGTCQLVGDDLFVTNPAR
LEQGIKDGLANSLLVKVNQIGTLSETLDAVDMAHRARYSAVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEELGDMARYPGAAIFG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=52.3255813953488, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=52.6806526806527, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=51.7482517482517, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=51.7482517482517, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=47.0725995316159, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=25.297619047619, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=25.297619047619, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=25.297619047619, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI310129182, Length=218, Percent_Identity=24.7706422018349, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI310110045, Length=218, Percent_Identity=24.7706422018349, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI310120572, Length=218, Percent_Identity=24.7706422018349, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=63.4433962264151, Blast_Score=523, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17536383, Length=430, Percent_Identity=53.4883720930233, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71995829, Length=430, Percent_Identity=53.4883720930233, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=47.8947368421053, Blast_Score=174, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=50.462962962963, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=50.462962962963, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=50.462962962963, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=50, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=50.2314814814815, Blast_Score=375, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=50, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=50, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=50, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=50, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=50, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=50, Blast_Score=385, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_SPHAL (Q1GVS8)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615577.1
- ProteinModelPortal:   Q1GVS8
- SMR:   Q1GVS8
- GeneID:   4081413
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0523
- NMPDR:   fig|317655.9.peg.480
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   SALA317655:SALA_0523-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44573; Mature: 44442

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIIDIHGREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAVELRDGDKGRYL
CEEEEEECCHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCH
GKGVTKAVAAVNGDIAEALVGLDAEDQREIDMAMIDLDGTPNKSRLGANAILGVSLAAAK
HHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHH
AAADARGLPLYRYVGGVSARTLPVPMMNIINGGEHADNPIDVQEFMIMPVGAGSIAEAVR
HHHCCCCCCHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
WGSEIFHTLKKGLSQKGLATAVGDEGGFAPNLASTRDALDFIAASVDQAGFKLGTDVVLA
HHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
LDCAATEFFRNGKYEISGEGLSLSPEQMAEYLAALVKDYPIKSIEDGMSEDDFAGWKALT
EHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHH
DLIGGTCQLVGDDLFVTNPARLEQGIKDGLANSLLVKVNQIGTLSETLDAVDMAHRARYS
HHHCCEEEEECCCEEEECHHHHHHHHHHHHHHHHEEEHHHCCCHHHHHHHHHHHHHHHHH
AVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEELGDMARYPGA
HHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AIFG
CCCC
>Mature Secondary Structure 
TAIIDIHGREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAVELRDGDKGRYL
EEEEEECCHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCH
GKGVTKAVAAVNGDIAEALVGLDAEDQREIDMAMIDLDGTPNKSRLGANAILGVSLAAAK
HHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHH
AAADARGLPLYRYVGGVSARTLPVPMMNIINGGEHADNPIDVQEFMIMPVGAGSIAEAVR
HHHCCCCCCHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
WGSEIFHTLKKGLSQKGLATAVGDEGGFAPNLASTRDALDFIAASVDQAGFKLGTDVVLA
HHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
LDCAATEFFRNGKYEISGEGLSLSPEQMAEYLAALVKDYPIKSIEDGMSEDDFAGWKALT
EHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHH
DLIGGTCQLVGDDLFVTNPARLEQGIKDGLANSLLVKVNQIGTLSETLDAVDMAHRARYS
HHHCCEEEEECCCEEEECHHHHHHHHHHHHHHHHEEEHHHCCCHHHHHHHHHHHHHHHHH
AVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEELGDMARYPGA
HHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AIFG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA