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The map label for this gene is pyk [H]

Identifier: 103485836

GI number: 103485836

Start: 343046

End: 344500

Strand: Direct

Name: pyk [H]

Synonym: Sala_0341

Alternate gene names: 103485836

Gene position: 343046-344500 (Clockwise)

Preceding gene: 103485821

Following gene: 103485837

Centisome position: 10.25

GC content: 67.15

Gene sequence:

>1455_bases
GTGGTTCAGAGCTTTACCCCCCGCCAGCGCAAGGTGCGTATCCTGGCGACGCTTGGCCCCGCGAGCGCCAATGCCGAGAT
GATCGCCGCGCTGCACCGTGCGGGCGCCGACGCCTTTCGCGTCAATATGAGCCATGGCGACCATGCGGGTCATGCAAAGG
TGATTGCGGCGATCCGGTCGCTTGAGAAGGAAACGGGGCGCCCGACGACGATCCTCGTCGACCTGCAGGGGCCAAAGCTG
CGCGTCGGGACGTTCAGGGATGGCCCCGCCGAGCTGGTCAAGGGTGCAGCGTTCATGCTCGATACCGACACGGCTCCCGG
CGACGCGACGCGCGTGCATCTGCCGCACCCCGAGCTGTTCGCGGCGCTCGAACCCGATACACGGCTGCTCATTGACGACG
GCAAGCTGGTGTTGCGCGTGAAGAGCGTGTCGGCCGAGCGGATCGAAACGATTGTCGAGGTCGGGGGCAAGATTTCGGAT
CGCAAGGGGGTCAATGTCCCCGACGTCGTCGTGCCGCTGGCGGCGCTCACCGAAAAGGATCGCAAGGATCTCGCCTTCGC
GCTCGAACAGCATGTCGACTGGATCGCGCTGTCCTTCGTGCAGCGACCCGAAGATGTCGCCGAAGCGCGGCGGCTGATCG
GCGGCAAGGCGGCGCTGCTCGTCAAGTTCGAGAAGCCGTCGGGGGTGCAGCGGATCGACGAGATTTTGGAACTTGCCGAC
GCGGCGATGGTCGCGCGCGGCGATCTGGGGGTCGAACTTCCTCCCGAAGCCGTGCCGCCGCTTCAGAAACGCATCGTCGC
GACCGCGCGGCGGATGGGCAAGCCGGTGGTGGTCGCGACGCAGATGCTCGAATCGATGATCGTCTCGCCCTCGCCGACGC
GCGCGGAAGTCAGCGATGTCGCGACCGCGGTTTATGACGGCGCCGACGCGATCATGCTGTCGGCCGAGACCGCCGCGGGC
GCGTGGCCGGTCGAAGCCGTCACCATGATGGATTCGATTGCGCGCTCGGTCGAAGGCGATCCCGATTATTTCCGCCGTCT
CCACTTCACCGAGACACAGCCCGACGCGACAACCGCCGACGCGCTGGCCGAGGCGGCGGGCAGCATCATCACGACGATCG
CCGCCGATGCGATCATCTGTTTCACTGCATCGGGATCGACCGCGCGCCGCGTCGCGCGCGAGCGGCCGGGCGCCCCCTTG
CTCGTGCTGACGCCGAGGAAGGAAGCAGCGCGGCGCATGGGGCTGCTGTGGGGCGCGCATGCCGTGCCGACCAGGGACAT
CGGCAGTTTCGAGGAGATGATCGCCAAGGGCAAGCGCATGGCGCTGCGCCACGGGATCGGCAAGGCGGGATCGAAGCTGG
TGATGATGGCGGGGGTGCCATTCGGCACGCCGGGATCGACCAATGTGCTCCACGTCGCGACGCTGACCGGCGACGAGCTG
CGTGGGTATAGCTGA

Upstream 100 bases:

>100_bases
ATGTTCGGACTTTCCTGAATAGCTATACGGTGGTTGCGCTGGGGCGCGCGACGCGCCATGCCTCGCACACCGTAACGGCA
ATCAGGCAAGCGGAGCAATC

Downstream 100 bases:

>100_bases
GCAGAGCTTCGCCGGAATGACGAACGGGGAAGGCGGCGGCATTGTGATTCAGTCCGGCACAAAAGTTACCGACCGATTCA
TAAAATATTCGATCTGATTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MVQSFTPRQRKVRILATLGPASANAEMIAALHRAGADAFRVNMSHGDHAGHAKVIAAIRSLEKETGRPTTILVDLQGPKL
RVGTFRDGPAELVKGAAFMLDTDTAPGDATRVHLPHPELFAALEPDTRLLIDDGKLVLRVKSVSAERIETIVEVGGKISD
RKGVNVPDVVVPLAALTEKDRKDLAFALEQHVDWIALSFVQRPEDVAEARRLIGGKAALLVKFEKPSGVQRIDEILELAD
AAMVARGDLGVELPPEAVPPLQKRIVATARRMGKPVVVATQMLESMIVSPSPTRAEVSDVATAVYDGADAIMLSAETAAG
AWPVEAVTMMDSIARSVEGDPDYFRRLHFTETQPDATTADALAEAAGSIITTIAADAIICFTASGSTARRVARERPGAPL
LVLTPRKEAARRMGLLWGAHAVPTRDIGSFEEMIAKGKRMALRHGIGKAGSKLVMMAGVPFGTPGSTNVLHVATLTGDEL
RGYS

Sequences:

>Translated_484_residues
MVQSFTPRQRKVRILATLGPASANAEMIAALHRAGADAFRVNMSHGDHAGHAKVIAAIRSLEKETGRPTTILVDLQGPKL
RVGTFRDGPAELVKGAAFMLDTDTAPGDATRVHLPHPELFAALEPDTRLLIDDGKLVLRVKSVSAERIETIVEVGGKISD
RKGVNVPDVVVPLAALTEKDRKDLAFALEQHVDWIALSFVQRPEDVAEARRLIGGKAALLVKFEKPSGVQRIDEILELAD
AAMVARGDLGVELPPEAVPPLQKRIVATARRMGKPVVVATQMLESMIVSPSPTRAEVSDVATAVYDGADAIMLSAETAAG
AWPVEAVTMMDSIARSVEGDPDYFRRLHFTETQPDATTADALAEAAGSIITTIAADAIICFTASGSTARRVARERPGAPL
LVLTPRKEAARRMGLLWGAHAVPTRDIGSFEEMIAKGKRMALRHGIGKAGSKLVMMAGVPFGTPGSTNVLHVATLTGDEL
RGYS
>Mature_484_residues
MVQSFTPRQRKVRILATLGPASANAEMIAALHRAGADAFRVNMSHGDHAGHAKVIAAIRSLEKETGRPTTILVDLQGPKL
RVGTFRDGPAELVKGAAFMLDTDTAPGDATRVHLPHPELFAALEPDTRLLIDDGKLVLRVKSVSAERIETIVEVGGKISD
RKGVNVPDVVVPLAALTEKDRKDLAFALEQHVDWIALSFVQRPEDVAEARRLIGGKAALLVKFEKPSGVQRIDEILELAD
AAMVARGDLGVELPPEAVPPLQKRIVATARRMGKPVVVATQMLESMIVSPSPTRAEVSDVATAVYDGADAIMLSAETAAG
AWPVEAVTMMDSIARSVEGDPDYFRRLHFTETQPDATTADALAEAAGSIITTIAADAIICFTASGSTARRVARERPGAPL
LVLTPRKEAARRMGLLWGAHAVPTRDIGSFEEMIAKGKRMALRHGIGKAGSKLVMMAGVPFGTPGSTNVLHVATLTGDEL
RGYS

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=38.7096774193548, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=38.7096774193548, Blast_Score=293, Evalue=4e-79,
Organism=Homo sapiens, GI33286418, Length=438, Percent_Identity=40.4109589041096, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI33286422, Length=436, Percent_Identity=40.8256880733945, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI33286420, Length=436, Percent_Identity=40.8256880733945, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=37.6306620209059, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=37.6306620209059, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=34.1772151898734, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=34.1772151898734, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI310128738, Length=213, Percent_Identity=33.8028169014084, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=36.3057324840764, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=35.625, Blast_Score=250, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=36.3449691991786, Blast_Score=290, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=37.4233128834356, Blast_Score=265, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=37.4233128834356, Blast_Score=264, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=37.4233128834356, Blast_Score=264, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=37.4233128834356, Blast_Score=264, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=36.9072164948454, Blast_Score=263, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6324923, Length=491, Percent_Identity=34.4195519348269, Blast_Score=249, Evalue=5e-67,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=36.105476673428, Blast_Score=278, Evalue=4e-75,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=36.105476673428, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24648966, Length=416, Percent_Identity=37.7403846153846, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24581235, Length=425, Percent_Identity=34.1176470588235, Blast_Score=232, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24646914, Length=311, Percent_Identity=35.3697749196142, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51579; Mature: 51579

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQSFTPRQRKVRILATLGPASANAEMIAALHRAGADAFRVNMSHGDHAGHAKVIAAIRS
CCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHH
LEKETGRPTTILVDLQGPKLRVGTFRDGPAELVKGAAFMLDTDTAPGDATRVHLPHPELF
HHHHCCCCEEEEEECCCCEEEEECCCCCHHHHHCCCEEEEECCCCCCCCEEEECCCHHHE
AALEPDTRLLIDDGKLVLRVKSVSAERIETIVEVGGKISDRKGVNVPDVVVPLAALTEKD
EEECCCCEEEEECCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCH
RKDLAFALEQHVDWIALSFVQRPEDVAEARRLIGGKAALLVKFEKPSGVQRIDEILELAD
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHH
AAMVARGDLGVELPPEAVPPLQKRIVATARRMGKPVVVATQMLESMIVSPSPTRAEVSDV
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHH
ATAVYDGADAIMLSAETAAGAWPVEAVTMMDSIARSVEGDPDYFRRLHFTETQPDATTAD
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHH
ALAEAAGSIITTIAADAIICFTASGSTARRVARERPGAPLLVLTPRKEAARRMGLLWGAH
HHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCC
AVPTRDIGSFEEMIAKGKRMALRHGIGKAGSKLVMMAGVPFGTPGSTNVLHVATLTGDEL
CCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCC
RGYS
CCCC
>Mature Secondary Structure
MVQSFTPRQRKVRILATLGPASANAEMIAALHRAGADAFRVNMSHGDHAGHAKVIAAIRS
CCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHH
LEKETGRPTTILVDLQGPKLRVGTFRDGPAELVKGAAFMLDTDTAPGDATRVHLPHPELF
HHHHCCCCEEEEEECCCCEEEEECCCCCHHHHHCCCEEEEECCCCCCCCEEEECCCHHHE
AALEPDTRLLIDDGKLVLRVKSVSAERIETIVEVGGKISDRKGVNVPDVVVPLAALTEKD
EEECCCCEEEEECCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCH
RKDLAFALEQHVDWIALSFVQRPEDVAEARRLIGGKAALLVKFEKPSGVQRIDEILELAD
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHH
AAMVARGDLGVELPPEAVPPLQKRIVATARRMGKPVVVATQMLESMIVSPSPTRAEVSDV
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHH
ATAVYDGADAIMLSAETAAGAWPVEAVTMMDSIARSVEGDPDYFRRLHFTETQPDATTAD
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHH
ALAEAAGSIITTIAADAIICFTASGSTARRVARERPGAPLLVLTPRKEAARRMGLLWGAH
HHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCC
AVPTRDIGSFEEMIAKGKRMALRHGIGKAGSKLVMMAGVPFGTPGSTNVLHVATLTGDEL
CCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCC
RGYS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA