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The map label for this gene is sdaA [H]

Identifier: 103485787

GI number: 103485787

Start: 290401

End: 291780

Strand: Direct

Name: sdaA [H]

Synonym: Sala_0292

Alternate gene names: 103485787

Gene position: 290401-291780 (Clockwise)

Preceding gene: 103485786

Following gene: 103485788

Centisome position: 8.68

GC content: 66.01

Gene sequence:

>1380_bases
ATGACGATCAGCCTGTTCGAACTGTTCAAAATCGGTGTCGGTCCCTCCAGCTCGCATACCGTCGGCCCGATGGTCGCGGC
GCGGCGCTTCACCGTCTGGCTCGACGAGCAGGCGCTGCTCGTCAGGACCGTGCATGTCGAGGCTGATCTCTACGGCTCGC
TTGCGCTGACGGGGAAGGGCCACGCCGCCGACACCGCGATCGTCGCGGGGCTTGCGGGCGAAATCCCCGCCTCGACCAGC
CTCGCCGCGATCAAGGCGCATTGGGAGCGCGCGGCCAGCGAGGGTTTCATCTATCTGCTCGGTCGTCAGCGCGTTCGCTT
CCAGCCGCAACGCGACCTTCATTTCCAGATGAAGCAGCGCCAGCCGTTCCACAGCAATGCCGTCAGCTTCACCGCGCGCG
ACGGCGATGGCGAAATCCTGGCCAAGCGCATGTATTTCTCGATCGGCGGCGGCTTTGTCGTCGACGAGGACGAGGCGGGA
CGCAACAGCCGCGGCGCCGAGGATGAGGTGGAACTGCCTTACGCCTTCACCTCGGGCGCCGACCTGCTCGCCATGGGCGC
CGTGTCGGGCAAGAGCTTTGCCGAGATGATGCTCGAAAATGAACTGGTGCATCGCAGCCTGGACGAGGTGAATGCCGGAC
TCGACGCCATCGCCGCCGCGATGGACGCCTCGATCGACGCCGGATGTTGCAGCACTGGCACCTTGCCCGGCGGGCTCAAG
GTCAAGCGCCGCGCGCCGCAGATCGCCGCCGACATCCGCGAACGGCATGAGGCCAATTTGTCGGACCCGATGGCGATGAT
GGACTGGATCAACCTGTGGGCGATGGCGGTGAATGAGGAAAATGCCGCGGGCGGCAAGGTCGTCACTGCGCCCACCAACG
GCGCGGCGGGGATCATCCCCGCGGTGATCCGCTACTATAAACGCAGTTATCGCGGCGACGCCGCGGGCGTACGCACTTTC
CTGCTCGCCGCGGGCGCGGTCGGCGCGCTCTATAAACGCAACGCCAGCATCTCGGGCGCCGAGGTCGGCTGTCAGGGTGA
GGTCGGCGTCGCCTGCTCGATGGCGGCGGCGGGGCTGACGGCGGCCTTGGGCGGCACCAACGCGCAGGTCGAAAATGCTG
CCGAGATCGGCATGGAACATAACCTCGGCCTCACCTGCGATCCCATCGGCGGCCTCGTCCAGATCCCTTGCATCGAGCGC
AACGCGATGGGCGCGATCAAGGCGATCGACGCCAGCCGCATCGCGATGATCGGCGACGGCACGCATGTCGTCAGCCTCGA
CACGGTGATCGCGACGATGCTGCAAACGGGCCGCGACATGCGCGACAAATATAAGGAAACGTCGAAGGGCGGGCTGGCGG
TCAGCGTGGTGGAGTGTTGA

Upstream 100 bases:

>100_bases
TTGCCACGTCGCTTCGCTCCTCGCGATGACGGCGACGGAAGCCGTCGGCCCCGAACGGCAGCCCTATCCCGATGACCGCG
ATCCCCCTATAGGCGCTGCC

Downstream 100 bases:

>100_bases
ACCCCGCCTTTGCTTCATCGTCATCCCGGACTTGATCCGGGATCCATGGCGGCGCCGCAGTCATGGACCTCGGATCAGAT
CGGCGCTTATCCCTTCGGGA

Product: L-serine dehydratase 1

Products: NA

Alternate protein names: SDH; L-serine deaminase; L-SD [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MTISLFELFKIGVGPSSSHTVGPMVAARRFTVWLDEQALLVRTVHVEADLYGSLALTGKGHAADTAIVAGLAGEIPASTS
LAAIKAHWERAASEGFIYLLGRQRVRFQPQRDLHFQMKQRQPFHSNAVSFTARDGDGEILAKRMYFSIGGGFVVDEDEAG
RNSRGAEDEVELPYAFTSGADLLAMGAVSGKSFAEMMLENELVHRSLDEVNAGLDAIAAAMDASIDAGCCSTGTLPGGLK
VKRRAPQIAADIRERHEANLSDPMAMMDWINLWAMAVNEENAAGGKVVTAPTNGAAGIIPAVIRYYKRSYRGDAAGVRTF
LLAAGAVGALYKRNASISGAEVGCQGEVGVACSMAAAGLTAALGGTNAQVENAAEIGMEHNLGLTCDPIGGLVQIPCIER
NAMGAIKAIDASRIAMIGDGTHVVSLDTVIATMLQTGRDMRDKYKETSKGGLAVSVVEC

Sequences:

>Translated_459_residues
MTISLFELFKIGVGPSSSHTVGPMVAARRFTVWLDEQALLVRTVHVEADLYGSLALTGKGHAADTAIVAGLAGEIPASTS
LAAIKAHWERAASEGFIYLLGRQRVRFQPQRDLHFQMKQRQPFHSNAVSFTARDGDGEILAKRMYFSIGGGFVVDEDEAG
RNSRGAEDEVELPYAFTSGADLLAMGAVSGKSFAEMMLENELVHRSLDEVNAGLDAIAAAMDASIDAGCCSTGTLPGGLK
VKRRAPQIAADIRERHEANLSDPMAMMDWINLWAMAVNEENAAGGKVVTAPTNGAAGIIPAVIRYYKRSYRGDAAGVRTF
LLAAGAVGALYKRNASISGAEVGCQGEVGVACSMAAAGLTAALGGTNAQVENAAEIGMEHNLGLTCDPIGGLVQIPCIER
NAMGAIKAIDASRIAMIGDGTHVVSLDTVIATMLQTGRDMRDKYKETSKGGLAVSVVEC
>Mature_458_residues
TISLFELFKIGVGPSSSHTVGPMVAARRFTVWLDEQALLVRTVHVEADLYGSLALTGKGHAADTAIVAGLAGEIPASTSL
AAIKAHWERAASEGFIYLLGRQRVRFQPQRDLHFQMKQRQPFHSNAVSFTARDGDGEILAKRMYFSIGGGFVVDEDEAGR
NSRGAEDEVELPYAFTSGADLLAMGAVSGKSFAEMMLENELVHRSLDEVNAGLDAIAAAMDASIDAGCCSTGTLPGGLKV
KRRAPQIAADIRERHEANLSDPMAMMDWINLWAMAVNEENAAGGKVVTAPTNGAAGIIPAVIRYYKRSYRGDAAGVRTFL
LAAGAVGALYKRNASISGAEVGCQGEVGVACSMAAAGLTAALGGTNAQVENAAEIGMEHNLGLTCDPIGGLVQIPCIERN
AMGAIKAIDASRIAMIGDGTHVVSLDTVIATMLQTGRDMRDKYKETSKGGLAVSVVEC

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI48994925, Length=458, Percent_Identity=52.8384279475983, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1788116, Length=456, Percent_Identity=50.219298245614, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1789161, Length=459, Percent_Identity=49.4553376906318, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 48305; Mature: 48173

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISLFELFKIGVGPSSSHTVGPMVAARRFTVWLDEQALLVRTVHVEADLYGSLALTGKG
CEEEHEEHHHHCCCCCCCCCCCHHHHHEEEEEEECCCEEEEEEEEEEEECCEEEEECCCC
HAADTAIVAGLAGEIPASTSLAAIKAHWERAASEGFIYLLGRQRVRFQPQRDLHFQMKQR
CCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHC
QPFHSNAVSFTARDGDGEILAKRMYFSIGGGFVVDEDEAGRNSRGAEDEVELPYAFTSGA
CCCCCCCEEEEEECCCCHHEEEEHEEEECCCEEEECCCCCCCCCCCCCCEECCEEECCCC
DLLAMGAVSGKSFAEMMLENELVHRSLDEVNAGLDAIAAAMDASIDAGCCSTGTLPGGLK
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCE
VKRRAPQIAADIRERHEANLSDPMAMMDWINLWAMAVNEENAAGGKVVTAPTNGAAGIIP
EHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCCCCCEEEECCCCCCCHHHH
AVIRYYKRSYRGDAAGVRTFLLAAGAVGALYKRNASISGAEVGCQGEVGVACSMAAAGLT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AALGGTNAQVENAAEIGMEHNLGLTCDPIGGLVQIPCIERNAMGAIKAIDASRIAMIGDG
HHCCCCCCCHHHHHHCCCCCCCCEEECCCCCEEEECEECCCCCCCEEEECCCEEEEEECC
THVVSLDTVIATMLQTGRDMRDKYKETSKGGLAVSVVEC
CEEEEHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure 
TISLFELFKIGVGPSSSHTVGPMVAARRFTVWLDEQALLVRTVHVEADLYGSLALTGKG
EEEHEEHHHHCCCCCCCCCCCHHHHHEEEEEEECCCEEEEEEEEEEEECCEEEEECCCC
HAADTAIVAGLAGEIPASTSLAAIKAHWERAASEGFIYLLGRQRVRFQPQRDLHFQMKQR
CCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHC
QPFHSNAVSFTARDGDGEILAKRMYFSIGGGFVVDEDEAGRNSRGAEDEVELPYAFTSGA
CCCCCCCEEEEEECCCCHHEEEEHEEEECCCEEEECCCCCCCCCCCCCCEECCEEECCCC
DLLAMGAVSGKSFAEMMLENELVHRSLDEVNAGLDAIAAAMDASIDAGCCSTGTLPGGLK
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCE
VKRRAPQIAADIRERHEANLSDPMAMMDWINLWAMAVNEENAAGGKVVTAPTNGAAGIIP
EHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCCCCCEEEECCCCCCCHHHH
AVIRYYKRSYRGDAAGVRTFLLAAGAVGALYKRNASISGAEVGCQGEVGVACSMAAAGLT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AALGGTNAQVENAAEIGMEHNLGLTCDPIGGLVQIPCIERNAMGAIKAIDASRIAMIGDG
HHCCCCCCCHHHHHHCCCCCCCCEEECCCCCEEEECEECCCCCCCEEEECCCEEEEEECC
THVVSLDTVIATMLQTGRDMRDKYKETSKGGLAVSVVEC
CEEEEHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]