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The map label for this gene is rph

Identifier: 103485749

GI number: 103485749

Start: 256982

End: 257698

Strand: Direct

Name: rph

Synonym: Sala_0254

Alternate gene names: 103485749

Gene position: 256982-257698 (Clockwise)

Preceding gene: 103485746

Following gene: 103485750

Centisome position: 7.68

GC content: 66.53

Gene sequence:

>717_bases
ATGCGCCCCTCCGGCCGCGCGGCAGACCAGATGCGTCCCATCGCCATCGAAACCAATTTCACCATCCATGCCGAGGGAAG
CGTGCTGGTCAGCTTCGGCAACACCAGGGTGCTGGTGACGGCGAGCGTCGAGGAAAAGGTGCCGCCCTTCCTGCGCGGCA
AGGGGCAGGGCTGGGTGACCGCCGAATATGGGATGCTGCCCCGCGCCACGCATACGCGCGGCAGCCGCGAGGCGGCGAAG
GGCAAACAGTCGGGGCGGACGCAGGAAATCCAGCGGCTCATCGGCCGCAGCTTGCGCGCCGTCGTCGACATGCAAAAGCT
CGGCGAGCGGCAGATCGTCATCGACTGTGACGTGATCCAGGCCGACGGCGGCACACGTACCGCATCGATCAGCGGCGCGT
GGGTTGCGCTGCGCCTTGCGGTCGACAAGCTGATCGAGGCGAAGCAGTTGACCGAAGACCCGATCGTCGACAGCGTCGCG
GCGATCTCGTGCGGCATTTACAAGGGAACGCCGGTGCTCGACCTCGATTATGACGAGGATTCGGTGGCCGAGGCCGACGG
CAATTTCGTGCTGACGGGCAAGGGACAGATCGTAGAGGTGCAGGCGAGCGCCGAGGGCGCGACCTATGACGAGGAGGGCC
TGCTCCGTCTGCTCCGCCTCGCGCGCATCGGCTGCGGCGAGATTTTCGCAGCGCAGGCGAAAGCAGTGGGGCGGTAG

Upstream 100 bases:

>100_bases
AGGATTTAGGGAGCGGGTGCGGCAAGAGCAAGCTATGCTCTGGCCAATCCCCCTGCGCGCGGCTAAGCGCCGCGGCACAG
CTTCACGAGGAGAAAGATTC

Downstream 100 bases:

>100_bases
CAAAAGCGTTGGAAAAGTTCCGCCGCAGTTGATAGGCTGGATTTATGAATGATCTCTCGTTCATGGACGTGGCCAATGTG
GCCGAGCTACGGCTGTGGCG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPSGRAADQMRPIAIETNFTIHAEGSVLVSFGNTRVLVTASVEEKVPPFLRGKGQGWVTAEYGMLPRATHTRGSREAAK
GKQSGRTQEIQRLIGRSLRAVVDMQKLGERQIVIDCDVIQADGGTRTASISGAWVALRLAVDKLIEAKQLTEDPIVDSVA
AISCGIYKGTPVLDLDYDEDSVAEADGNFVLTGKGQIVEVQASAEGATYDEEGLLRLLRLARIGCGEIFAAQAKAVGR

Sequences:

>Translated_238_residues
MRPSGRAADQMRPIAIETNFTIHAEGSVLVSFGNTRVLVTASVEEKVPPFLRGKGQGWVTAEYGMLPRATHTRGSREAAK
GKQSGRTQEIQRLIGRSLRAVVDMQKLGERQIVIDCDVIQADGGTRTASISGAWVALRLAVDKLIEAKQLTEDPIVDSVA
AISCGIYKGTPVLDLDYDEDSVAEADGNFVLTGKGQIVEVQASAEGATYDEEGLLRLLRLARIGCGEIFAAQAKAVGR
>Mature_238_residues
MRPSGRAADQMRPIAIETNFTIHAEGSVLVSFGNTRVLVTASVEEKVPPFLRGKGQGWVTAEYGMLPRATHTRGSREAAK
GKQSGRTQEIQRLIGRSLRAVVDMQKLGERQIVIDCDVIQADGGTRTASISGAWVALRLAVDKLIEAKQLTEDPIVDSVA
AISCGIYKGTPVLDLDYDEDSVAEADGNFVLTGKGQIVEVQASAEGATYDEEGLLRLLRLARIGCGEIFAAQAKAVGR

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=62.6794258373206, Blast_Score=272, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_SPHAL (Q1GWJ5)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615310.1
- ProteinModelPortal:   Q1GWJ5
- SMR:   Q1GWJ5
- GeneID:   4083112
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0254
- NMPDR:   fig|317655.9.peg.233
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   SALA317655:SALA_0254-MONOMER
- BRENDA:   2.7.7.56
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25509; Mature: 25509

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSGRAADQMRPIAIETNFTIHAEGSVLVSFGNTRVLVTASVEEKVPPFLRGKGQGWVT
CCCCCCCHHCCCCEEEECCEEEEECCEEEEEECCEEEEEEECCHHHCCHHHCCCCCCEEE
AEYGMLPRATHTRGSREAAKGKQSGRTQEIQRLIGRSLRAVVDMQKLGERQIVIDCDVIQ
ECCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASISGAWVALRLAVDKLIEAKQLTEDPIVDSVAAISCGIYKGTPVLDLDYDED
CCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCEEEEECCCCC
SVAEADGNFVLTGKGQIVEVQASAEGATYDEEGLLRLLRLARIGCGEIFAAQAKAVGR
CHHCCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MRPSGRAADQMRPIAIETNFTIHAEGSVLVSFGNTRVLVTASVEEKVPPFLRGKGQGWVT
CCCCCCCHHCCCCEEEECCEEEEECCEEEEEECCEEEEEEECCHHHCCHHHCCCCCCEEE
AEYGMLPRATHTRGSREAAKGKQSGRTQEIQRLIGRSLRAVVDMQKLGERQIVIDCDVIQ
ECCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTASISGAWVALRLAVDKLIEAKQLTEDPIVDSVAAISCGIYKGTPVLDLDYDED
CCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCEEEEECCCCC
SVAEADGNFVLTGKGQIVEVQASAEGATYDEEGLLRLLRLARIGCGEIFAAQAKAVGR
CHHCCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA