Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 103485746

GI number: 103485746

Start: 254531

End: 255073

Strand: Direct

Name: def [H]

Synonym: Sala_0251

Alternate gene names: 103485746

Gene position: 254531-255073 (Clockwise)

Preceding gene: 103485745

Following gene: 103485749

Centisome position: 7.61

GC content: 61.33

Gene sequence:

>543_bases
ATGGCATCCATGGCCATCCTACCCATCATCGAGACGCCGGACCCCCGGCTGCGCGTCATTTCCAAGCCCGTCGAGACCTT
CGACGCCGAGCTGAAGCAGCTTGTCGCCGACATGTTCGAGACGATGTACGACGCCCCAGGCATCGGGCTCGCCGCGATCC
AGGTCGGGGTGCCGAAGCGCATCCTGGTGATCGACCTGCAGGAAGCCGATCCCGAAGACCCCGAGGGCAAGAAGGTGATC
CGAACCCCTCGCGTCTTCATCAACCCCGTCTTTTCGGACTTTTCGGACGAGCATAGCGTCTATCAGGAAGGCTGCCTGTC
GGTCCCCGAACAATATGCCGATGTGACGCGCCCCGCCGAAGTCACCGTCGACTGGCAGGACGAGGACGGCAAGCATCACC
AGGAACGCATGACCGGCCTGATGGCGACGTGCATCCAGCACGAGCATGACCATCTGGAAGGTATTTTGTTCATCGACCAT
CTGTCGCGGCTGAAGCGCGAGATGGTGCTGAAAAAGCTCGCAAAGCTGCGCAAGGCGGCGTGA

Upstream 100 bases:

>100_bases
TCTCGATGAGGGGACGCTGGCGCAGGCGCTGCGGGCAAGAAGGCCAGTGGGCTGAGCCTGCATCGTGCCGATCATTGACC
GCCCGGCCGCGCATACCTAA

Downstream 100 bases:

>100_bases
TTGGTTCTTCCCTCTCCCCTTGCGGGAGAGGGCGGCGAGACTTGCCAGCTTGCTGGCTAGTCGCAGCGGGAGAGGGGTCT
CGCGCGCTTGTGCGCCAAGA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MASMAILPIIETPDPRLRVISKPVETFDAELKQLVADMFETMYDAPGIGLAAIQVGVPKRILVIDLQEADPEDPEGKKVI
RTPRVFINPVFSDFSDEHSVYQEGCLSVPEQYADVTRPAEVTVDWQDEDGKHHQERMTGLMATCIQHEHDHLEGILFIDH
LSRLKREMVLKKLAKLRKAA

Sequences:

>Translated_180_residues
MASMAILPIIETPDPRLRVISKPVETFDAELKQLVADMFETMYDAPGIGLAAIQVGVPKRILVIDLQEADPEDPEGKKVI
RTPRVFINPVFSDFSDEHSVYQEGCLSVPEQYADVTRPAEVTVDWQDEDGKHHQERMTGLMATCIQHEHDHLEGILFIDH
LSRLKREMVLKKLAKLRKAA
>Mature_179_residues
ASMAILPIIETPDPRLRVISKPVETFDAELKQLVADMFETMYDAPGIGLAAIQVGVPKRILVIDLQEADPEDPEGKKVIR
TPRVFINPVFSDFSDEHSVYQEGCLSVPEQYADVTRPAEVTVDWQDEDGKHHQERMTGLMATCIQHEHDHLEGILFIDHL
SRLKREMVLKKLAKLRKAA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=33.9506172839506, Blast_Score=85, Evalue=4e-17,
Organism=Escherichia coli, GI1789682, Length=173, Percent_Identity=46.242774566474, Blast_Score=143, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24645728, Length=159, Percent_Identity=32.7044025157233, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24645726, Length=140, Percent_Identity=32.8571428571429, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20369; Mature: 20238

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASMAILPIIETPDPRLRVISKPVETFDAELKQLVADMFETMYDAPGIGLAAIQVGVPKR
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCE
ILVIDLQEADPEDPEGKKVIRTPRVFINPVFSDFSDEHSVYQEGCLSVPEQYADVTRPAE
EEEEEECCCCCCCCCCCEEEECCHHHCCHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCE
VTVDWQDEDGKHHQERMTGLMATCIQHEHDHLEGILFIDHLSRLKREMVLKKLAKLRKAA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASMAILPIIETPDPRLRVISKPVETFDAELKQLVADMFETMYDAPGIGLAAIQVGVPKR
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCE
ILVIDLQEADPEDPEGKKVIRTPRVFINPVFSDFSDEHSVYQEGCLSVPEQYADVTRPAE
EEEEEECCCCCCCCCCCEEEECCHHHCCHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCE
VTVDWQDEDGKHHQERMTGLMATCIQHEHDHLEGILFIDHLSRLKREMVLKKLAKLRKAA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA