BacMap

Definition	Ruegeria sp. TM1040, complete genome.
Accession	NC_008044
Length	3,200,938

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The map label for this gene is fadB1 [H]

Identifier: 99080971

GI number: 99080971

Start: 1214788

End: 1215567

Strand: Direct

Name: fadB1 [H]

Synonym: TM1040_1130

Alternate gene names: 99080971

Gene position: 1214788-1215567 (Clockwise)

Preceding gene: 99080970

Following gene: 99080975

Centisome position: 37.95

GC content: 63.21

Gene sequence:

>780_bases
ATGGACTACCGCGACATTCAGTATTCGCTTGAGAACGGGCTTGCGACCGTCACTTTGAACCGCCCGCAGAGCATGAATGC
GCTCACCAGCCAGATGCGGGCGGAAATCACCCATGCGATGAAAACCGCCGCCGAAGAGGCGCGTGCGGTGGTGATCACCG
GCGCTGGCAATGCCTTTTGTACCGGTCAGGACCTGAAGGATGCCTCCTCGAGCGGCAACCAGATCGATCTGGAGCGTACG
CTGCGTGACGAATACACGCCCATGCTGGAAGCGATCTACACCTGTCCAGTGCCGACGATCTCTGCTGTGAATGGTCCGGC
AGCAGGGGCGGGCGCCAATCTGGCACTGGCGGCGGATGTGGTGATCGCAACCGAGAGCGCCTATTTCCTGCAGGCCTTTG
CCAAGATCGGGTTGATGCCGGACGCCGGCGGTACTTGGTTCATGCCGCGCCAGATGGGGCTGGCCAAGGCCATGGGCGCG
GCGCTGTTTGCCGACAAGATCACCGCCCGTCAGGCGAGCGATTGGGGCATGATCTGGGAAGCGGTTGCGGATGACGGGTT
TGAGGCGCATTGGCGCGCCCGTGCCGCGCACCTGGCCTCTGGTCCCTCCAAGGCCTTTGGCGCCATCAAGCAGGCGCTGC
GCCAATCCACCACCAACAGCCTGCCAGATCAGCTGTCGCTGGAGGCGCATCTGCAGGGGGAACTGGGCCGCAGCCGCGAT
TTCATGGAGGGCGTCACCGCCTTTGTGGAAAAGCGCAAACCGGATTTCGAGGGGCGCTGA

Upstream 100 bases:

>100_bases
AGCGCAAGAAAATCCCTAAATAGCCTGACGCGGCGTTTGTCTTTTCGAGGTCGGATGCTTAGGTCAACATGCGCATAACT
CGAACAGGGATATTTCACGC

Downstream 100 bases:

>100_bases
GGCCCCACTTGTTATGATCCCACGAAAAAGCCGCCCCCGATTGAGGGCGGCTTTTGTTTGCTCAAAGACCGAAGATCACG
CAGAGACCAGGGCCACGTTT

Product: short chain enoyl-CoA hydratase / enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MDYRDIQYSLENGLATVTLNRPQSMNALTSQMRAEITHAMKTAAEEARAVVITGAGNAFCTGQDLKDASSSGNQIDLERT
LRDEYTPMLEAIYTCPVPTISAVNGPAAGAGANLALAADVVIATESAYFLQAFAKIGLMPDAGGTWFMPRQMGLAKAMGA
ALFADKITARQASDWGMIWEAVADDGFEAHWRARAAHLASGPSKAFGAIKQALRQSTTNSLPDQLSLEAHLQGELGRSRD
FMEGVTAFVEKRKPDFEGR

Sequences:

>Translated_259_residues
MDYRDIQYSLENGLATVTLNRPQSMNALTSQMRAEITHAMKTAAEEARAVVITGAGNAFCTGQDLKDASSSGNQIDLERT
LRDEYTPMLEAIYTCPVPTISAVNGPAAGAGANLALAADVVIATESAYFLQAFAKIGLMPDAGGTWFMPRQMGLAKAMGA
ALFADKITARQASDWGMIWEAVADDGFEAHWRARAAHLASGPSKAFGAIKQALRQSTTNSLPDQLSLEAHLQGELGRSRD
FMEGVTAFVEKRKPDFEGR
>Mature_259_residues
MDYRDIQYSLENGLATVTLNRPQSMNALTSQMRAEITHAMKTAAEEARAVVITGAGNAFCTGQDLKDASSSGNQIDLERT
LRDEYTPMLEAIYTCPVPTISAVNGPAAGAGANLALAADVVIATESAYFLQAFAKIGLMPDAGGTWFMPRQMGLAKAMGA
ALFADKITARQASDWGMIWEAVADDGFEAHWRARAAHLASGPSKAFGAIKQALRQSTTNSLPDQLSLEAHLQGELGRSRD
FMEGVTAFVEKRKPDFEGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=30.6451612903226, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI45643119, Length=219, Percent_Identity=29.2237442922374, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI260275230, Length=219, Percent_Identity=29.2237442922374, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI260274832, Length=219, Percent_Identity=29.2237442922374, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI25453481, Length=260, Percent_Identity=26.9230769230769, Blast_Score=94, Evalue=8e-20,
Organism=Homo sapiens, GI51538573, Length=260, Percent_Identity=26.9230769230769, Blast_Score=94, Evalue=8e-20,
Organism=Homo sapiens, GI4757968, Length=262, Percent_Identity=25.9541984732824, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI49355787, Length=262, Percent_Identity=25.9541984732824, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI4757966, Length=204, Percent_Identity=29.9019607843137, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI51538581, Length=204, Percent_Identity=29.9019607843137, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI221136756, Length=267, Percent_Identity=28.0898876404494, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI221136753, Length=267, Percent_Identity=28.0898876404494, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI221307494, Length=267, Percent_Identity=28.0898876404494, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI116235487, Length=258, Percent_Identity=25.5813953488372, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI37594471, Length=143, Percent_Identity=34.2657342657343, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI37594469, Length=143, Percent_Identity=34.2657342657343, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=29.2, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI31542718, Length=262, Percent_Identity=24.4274809160305, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=26.953125, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI20127408, Length=183, Percent_Identity=25.6830601092896, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI157694516, Length=244, Percent_Identity=25.4098360655738, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI213417737, Length=244, Percent_Identity=25.4098360655738, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI68989263, Length=185, Percent_Identity=28.1081081081081, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=44.5736434108527, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=36.3265306122449, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=30.859375, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=27.7566539923954, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI87082183, Length=267, Percent_Identity=25.0936329588015, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1788682, Length=177, Percent_Identity=27.1186440677966, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790281, Length=175, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=30.2788844621514, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=29.3877551020408, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17535521, Length=248, Percent_Identity=29.0322580645161, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17549921, Length=251, Percent_Identity=28.2868525896414, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=243, Percent_Identity=26.7489711934156, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=26.4069264069264, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=257, Percent_Identity=28.7937743190661, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=259, Percent_Identity=25.8687258687259, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=199, Percent_Identity=25.6281407035176, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=28.1632653061224, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=28.1632653061224, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=27.4509803921569, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24654903, Length=181, Percent_Identity=26.5193370165746, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920382, Length=258, Percent_Identity=28.6821705426357, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28571729, Length=139, Percent_Identity=31.6546762589928, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28571730, Length=139, Percent_Identity=31.6546762589928, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19922422, Length=249, Percent_Identity=24.0963855421687, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21357171, Length=263, Percent_Identity=25.0950570342205, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27793; Mature: 27793

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYRDIQYSLENGLATVTLNRPQSMNALTSQMRAEITHAMKTAAEEARAVVITGAGNAFC
CCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEE
TGQDLKDASSSGNQIDLERTLRDEYTPMLEAIYTCPVPTISAVNGPAAGAGANLALAADV
CCCCCHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEEE
VIATESAYFLQAFAKIGLMPDAGGTWFMPRQMGLAKAMGAALFADKITARQASDWGMIWE
EEECCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AVADDGFEAHWRARAAHLASGPSKAFGAIKQALRQSTTNSLPDQLSLEAHLQGELGRSRD
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHH
FMEGVTAFVEKRKPDFEGR
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDYRDIQYSLENGLATVTLNRPQSMNALTSQMRAEITHAMKTAAEEARAVVITGAGNAFC
CCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEE
TGQDLKDASSSGNQIDLERTLRDEYTPMLEAIYTCPVPTISAVNGPAAGAGANLALAADV
CCCCCHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEEE
VIATESAYFLQAFAKIGLMPDAGGTWFMPRQMGLAKAMGAALFADKITARQASDWGMIWE
EEECCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
AVADDGFEAHWRARAAHLASGPSKAFGAIKQALRQSTTNSLPDQLSLEAHLQGELGRSRD
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHH
FMEGVTAFVEKRKPDFEGR
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1743516 [H]