| Definition | Ruegeria sp. TM1040, complete genome. |
|---|---|
| Accession | NC_008044 |
| Length | 3,200,938 |
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The map label for this gene is acnB [H]
Identifier: 99080867
GI number: 99080867
Start: 1097776
End: 1100565
Strand: Direct
Name: acnB [H]
Synonym: TM1040_1026
Alternate gene names: 99080867
Gene position: 1097776-1100565 (Clockwise)
Preceding gene: 99080865
Following gene: 99080868
Centisome position: 34.3
GC content: 59.43
Gene sequence:
>2790_bases ATGAGCTTGTACACAGACTACCTAAATGAGATCGAAACCCGCAAAGGCGAGGGGCTGCACCCCAAGCCCATCGACGATGG CGCGCTGGTTGCGGAATTGATTGCGCAGATCAAGGACACCGGGAGCGAGCATCGTGAAGACTCTCTGAAGTTCTTCATTT ACAACACCTTGCCCGGCACCACGAGCGCTGCAGGTGTCAAGGCGGCGTTTCTCAAAGAGATCATTCTGGGCGAGGCCGAG GTGGCTGAAATCACGCCCGAGTTCGCCTTTGAACTGCTGTCGCACATGAAGGGTGGTCCCTCGGTCGAGGTGCTTCTGGA TCTGGCCCTCGGTGAGGATGCAGGGATCGCCCAGCAGGCCGCAGACGTGCTGAAGACACAGGTGTTCCTCTATGAGGCGG ACACCGATCGTCTCGAAGCAGCTTACAAGGCTGGCAATGCGATTGCGCGTGAGATCCTCGAGAGCTACGCCAAGGCCGAG TTCTTTACCAAGCTTCCCGATGTGGATGATGAGATCAAGGTCGTGACCTACATCGCCGCCGAAGGCGATATCTCCACCGA CCTTCTGTCCCCGGGCAATCAGGCGCACTCGCGTTCGGATCGGGAACTGCATGGCAAATGCATGATCTCCGAGGCGGCGC AAAAGGAGATCGAGGCGCTGAAGCTGCAGCACCCCGACAAGCGCGTGATGCTGATCGCGGAAAAAGGCACCATGGGCGTG GGCTCCTCGCGGATGTCCGGCGTCAACAACGTGGCGCTCTGGACCGGCAAACAAGCCAGCCCCTATGTGCCGTTTGTGAA CTATGCCCCTGTGGTTGCGGGCACCAATGGCATCTCGCCGATTTTCCTCACCACCGTGGGCGTGACCGGCGGCATTGGCC TCGACCTCAAGAACTGGGTCAAGAAGGTCGATAGCGACGGCAAGGCGATCCTCAACAACGACGGCAACCCGGTGCTGGAG CAGAAGTATTCTGTTGAAACCGGCACGGTGCTGAAGATCGACACCAAGAACAAGAAGCTCCTGAGCGAAGACGGCGAGGA GCTGGTGGATGTCTCGTCCGCGTTTACGCCCCAGAAGGTCGAGTTCATGAAGGCGGGCAGCTCTTATGCCATCGTCTTTG GCAAGAAACTGCAGACATTTGCGGCCGAGACCCTCGGCATCGAGGCGCCGCTGGTCTTTGCACCGGCGAAAGAAGTCAGC CACGAGGGCCAGGGCCTGACTGCGGTTGAAAAGATCTTTAACCGCAATGCCGTGGGCAGCACGCCGGGCAAGACGCTCCA TGCGGGCTCTGACGTGCGCGTGAAGGTCAACATTGTCGGCTCTCAGGACACCACTGGTCTCATGACCTCGCAAGAACTGG AGGCCATGGCGGCCACCGTGATCTCGCCGACCGTGGATGGGGCCTATCAGTCCGGCTGTCACACCGCGTCCGTGTGGGAC AAGAAGGCACAGGCCAACATTCCGCGTCTGATGAAGTTCATGAACAACTTCGGCCTGATCACCGCGCGCGATCCAAAGGA TCAGTATCACGCGATGACCGACGTGATCCATAAGGTGCTGAACGACCTCACCGTGGATGACTGGGACATCATCATCGGCG GCGACAGCCACACCCGCATGTCCAAAGGCGTGGCCTTTGGTGCGGACTCGGGTACCGTGGCGCTGGCGCTGGCAACCGGT GAGGCGACCATGCCAATCCCCGAGTCGGTCAAAGTGACCTTCAAGGGCAAGATGGCCGACCATATGGACTTCCGCGATGT GGTTCACGCCACCCAGGCGCAGATGCTCAAGCAGCATGGCGACAACGTCTTCCAGGGCCGCGTCATCGAGGTCCACATCG GCACGCTCCTGGCAGACCAGGCCTTTACCTTCACCGACTGGACGGCCGAGATGAAGGCCAAGGCGTCGATCTGTATCTCC AATGACGATACGCTCATCGGCTCGCTCGAGCTCGCCAAGAGCCGCATCCAGATCATGATCGACAAAGGCATGGACAACGA GGCGGGTACCCTCCAGGGGCTGATCGACAAGGCCGACAAGCGCATCGCCGAGATCCGCTCCGGTGAGAAGCCGGCCCTGT CGCCCGACGACAGCGCCAAGTATTACGCCGAAGTGGTGGTCGATCTCGACGAGATCAACGAGCCGATGATTGCCGACCCG GACGTCAACAACGAGGACGTCTCCAAGCGCTACACCCACGACACCATCCGTCCGATCTCTTTCTATAACGCAGAGAAAAA GGTCGATCTTGGCTTTGTGGGCTCCTGCATGGTGCACAAGGGAGACATGAAGATCGTGGCGCAGATGCTCAAGAACCTCG AGAAAGCCAACGGCAAGGTCGAGTTCAAGGCTCCGCTCGTGGTGGCCGCGCCGACCTACAACATCATCGACGAGATGAAG GCCGAAGGTGACTGGGAGATCCTGCAGAAATACTCGGGCTTTGAGTTCGACGATCTGTTCCCGAAATCGCAGGCGCGGAC CGAGTATGAAAACATCCTCTATCTGGAGCGTCCGGGCTGTAACCTCTGCATGGGCAACCAGGAAAAGGCCGCCAAGGGTG ACACCGTTCTGGCCACATCGACCCGCCTGTTCCAGGGCCGTGTGGTGGAAGATGCCGCCGAGAAGAAAGGCGAGAGCCTT CTTGCCTCGACCCCGGTTGTGGTGCTCTCGGCCATTCTGGGTCGCACGCCAACGATCGAGGAATACAAGACCGCCGTGGA GGGTATTGACCTCACCAAATTTGCCCCTCCGGTCGAAAAACCGGCAGGAACGCGCTCGGCGCATTTCTAA
Upstream 100 bases:
>100_bases ATTGGTATACTGCCGCATACAGCAAGCTTGTCACGGCTCGCCAAGAGCGTTTGCGACCCCCGTTTCAGGCTTTTTCTTAC ACAGGATCGTCGGGACCGTC
Downstream 100 bases:
>100_bases ACACCTCTGCGCGGCGCAGACGGCTTTCTGGGGGCAATATGCTCACAGGTGACGTCCGCGGCGCGCAGTCTGGAAAGGTC AGACCGACCGCCAGAATCCG
Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase
Products: NA
Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]
Number of amino acids: Translated: 929; Mature: 928
Protein sequence:
>929_residues MSLYTDYLNEIETRKGEGLHPKPIDDGALVAELIAQIKDTGSEHREDSLKFFIYNTLPGTTSAAGVKAAFLKEIILGEAE VAEITPEFAFELLSHMKGGPSVEVLLDLALGEDAGIAQQAADVLKTQVFLYEADTDRLEAAYKAGNAIAREILESYAKAE FFTKLPDVDDEIKVVTYIAAEGDISTDLLSPGNQAHSRSDRELHGKCMISEAAQKEIEALKLQHPDKRVMLIAEKGTMGV GSSRMSGVNNVALWTGKQASPYVPFVNYAPVVAGTNGISPIFLTTVGVTGGIGLDLKNWVKKVDSDGKAILNNDGNPVLE QKYSVETGTVLKIDTKNKKLLSEDGEELVDVSSAFTPQKVEFMKAGSSYAIVFGKKLQTFAAETLGIEAPLVFAPAKEVS HEGQGLTAVEKIFNRNAVGSTPGKTLHAGSDVRVKVNIVGSQDTTGLMTSQELEAMAATVISPTVDGAYQSGCHTASVWD KKAQANIPRLMKFMNNFGLITARDPKDQYHAMTDVIHKVLNDLTVDDWDIIIGGDSHTRMSKGVAFGADSGTVALALATG EATMPIPESVKVTFKGKMADHMDFRDVVHATQAQMLKQHGDNVFQGRVIEVHIGTLLADQAFTFTDWTAEMKAKASICIS NDDTLIGSLELAKSRIQIMIDKGMDNEAGTLQGLIDKADKRIAEIRSGEKPALSPDDSAKYYAEVVVDLDEINEPMIADP DVNNEDVSKRYTHDTIRPISFYNAEKKVDLGFVGSCMVHKGDMKIVAQMLKNLEKANGKVEFKAPLVVAAPTYNIIDEMK AEGDWEILQKYSGFEFDDLFPKSQARTEYENILYLERPGCNLCMGNQEKAAKGDTVLATSTRLFQGRVVEDAAEKKGESL LASTPVVVLSAILGRTPTIEEYKTAVEGIDLTKFAPPVEKPAGTRSAHF
Sequences:
>Translated_929_residues MSLYTDYLNEIETRKGEGLHPKPIDDGALVAELIAQIKDTGSEHREDSLKFFIYNTLPGTTSAAGVKAAFLKEIILGEAE VAEITPEFAFELLSHMKGGPSVEVLLDLALGEDAGIAQQAADVLKTQVFLYEADTDRLEAAYKAGNAIAREILESYAKAE FFTKLPDVDDEIKVVTYIAAEGDISTDLLSPGNQAHSRSDRELHGKCMISEAAQKEIEALKLQHPDKRVMLIAEKGTMGV GSSRMSGVNNVALWTGKQASPYVPFVNYAPVVAGTNGISPIFLTTVGVTGGIGLDLKNWVKKVDSDGKAILNNDGNPVLE QKYSVETGTVLKIDTKNKKLLSEDGEELVDVSSAFTPQKVEFMKAGSSYAIVFGKKLQTFAAETLGIEAPLVFAPAKEVS HEGQGLTAVEKIFNRNAVGSTPGKTLHAGSDVRVKVNIVGSQDTTGLMTSQELEAMAATVISPTVDGAYQSGCHTASVWD KKAQANIPRLMKFMNNFGLITARDPKDQYHAMTDVIHKVLNDLTVDDWDIIIGGDSHTRMSKGVAFGADSGTVALALATG EATMPIPESVKVTFKGKMADHMDFRDVVHATQAQMLKQHGDNVFQGRVIEVHIGTLLADQAFTFTDWTAEMKAKASICIS NDDTLIGSLELAKSRIQIMIDKGMDNEAGTLQGLIDKADKRIAEIRSGEKPALSPDDSAKYYAEVVVDLDEINEPMIADP DVNNEDVSKRYTHDTIRPISFYNAEKKVDLGFVGSCMVHKGDMKIVAQMLKNLEKANGKVEFKAPLVVAAPTYNIIDEMK AEGDWEILQKYSGFEFDDLFPKSQARTEYENILYLERPGCNLCMGNQEKAAKGDTVLATSTRLFQGRVVEDAAEKKGESL LASTPVVVLSAILGRTPTIEEYKTAVEGIDLTKFAPPVEKPAGTRSAHF >Mature_928_residues SLYTDYLNEIETRKGEGLHPKPIDDGALVAELIAQIKDTGSEHREDSLKFFIYNTLPGTTSAAGVKAAFLKEIILGEAEV AEITPEFAFELLSHMKGGPSVEVLLDLALGEDAGIAQQAADVLKTQVFLYEADTDRLEAAYKAGNAIAREILESYAKAEF FTKLPDVDDEIKVVTYIAAEGDISTDLLSPGNQAHSRSDRELHGKCMISEAAQKEIEALKLQHPDKRVMLIAEKGTMGVG SSRMSGVNNVALWTGKQASPYVPFVNYAPVVAGTNGISPIFLTTVGVTGGIGLDLKNWVKKVDSDGKAILNNDGNPVLEQ KYSVETGTVLKIDTKNKKLLSEDGEELVDVSSAFTPQKVEFMKAGSSYAIVFGKKLQTFAAETLGIEAPLVFAPAKEVSH EGQGLTAVEKIFNRNAVGSTPGKTLHAGSDVRVKVNIVGSQDTTGLMTSQELEAMAATVISPTVDGAYQSGCHTASVWDK KAQANIPRLMKFMNNFGLITARDPKDQYHAMTDVIHKVLNDLTVDDWDIIIGGDSHTRMSKGVAFGADSGTVALALATGE ATMPIPESVKVTFKGKMADHMDFRDVVHATQAQMLKQHGDNVFQGRVIEVHIGTLLADQAFTFTDWTAEMKAKASICISN DDTLIGSLELAKSRIQIMIDKGMDNEAGTLQGLIDKADKRIAEIRSGEKPALSPDDSAKYYAEVVVDLDEINEPMIADPD VNNEDVSKRYTHDTIRPISFYNAEKKVDLGFVGSCMVHKGDMKIVAQMLKNLEKANGKVEFKAPLVVAAPTYNIIDEMKA EGDWEILQKYSGFEFDDLFPKSQARTEYENILYLERPGCNLCMGNQEKAAKGDTVLATSTRLFQGRVVEDAAEKKGESLL ASTPVVVLSAILGRTPTIEEYKTAVEGIDLTKFAPPVEKPAGTRSAHF
Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]
COG id: COG1049
COG function: function code C; Aconitase B
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aconitase/IPM isomerase family [H]
Homologues:
Organism=Escherichia coli, GI2367097, Length=930, Percent_Identity=32.1505376344086, Blast_Score=329, Evalue=5e-91,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR015931 - InterPro: IPR015937 - InterPro: IPR001030 - InterPro: IPR015928 - InterPro: IPR018136 - InterPro: IPR004406 - InterPro: IPR015930 - InterPro: IPR015933 - InterPro: IPR015929 [H]
Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]
EC number: =4.2.1.3; =4.2.1.99 [H]
Molecular weight: Translated: 100779; Mature: 100647
Theoretical pI: Translated: 4.81; Mature: 4.81
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSLYTDYLNEIETRKGEGLHPKPIDDGALVAELIAQIKDTGSEHREDSLKFFIYNTLPGT CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCEEEEEEECCCCC TSAAGVKAAFLKEIILGEAEVAEITPEFAFELLSHMKGGPSVEVLLDLALGEDAGIAQQA CCCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHH ADVLKTQVFLYEADTDRLEAAYKAGNAIAREILESYAKAEFFTKLPDVDDEIKVVTYIAA HHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEE EGDISTDLLSPGNQAHSRSDRELHGKCMISEAAQKEIEALKLQHPDKRVMLIAEKGTMGV CCCCCHHHCCCCCHHCCCCCCHHCCCEEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC GSSRMSGVNNVALWTGKQASPYVPFVNYAPVVAGTNGISPIFLTTVGVTGGIGLDLKNWV CCHHHCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCCCCCHHHHH KKVDSDGKAILNNDGNPVLEQKYSVETGTVLKIDTKNKKLLSEDGEELVDVSSAFTPQKV HHHCCCCCEEECCCCCCHHHHEECCCCCCEEEEECCCCHHHHCCCHHHHHHHCCCCCHHH EFMKAGSSYAIVFGKKLQTFAAETLGIEAPLVFAPAKEVSHEGQGLTAVEKIFNRNAVGS HHHHCCCCEEEEECCHHHHHHHHHHCCCCCEEECCHHHHCCCCCCHHHHHHHHCCCCCCC TPGKTLHAGSDVRVKVNIVGSQDTTGLMTSQELEAMAATVISPTVDGAYQSGCHTASVWD CCCCEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHC KKAQANIPRLMKFMNNFGLITARDPKDQYHAMTDVIHKVLNDLTVDDWDIIIGGDSHTRM CHHHCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH SKGVAFGADSGTVALALATGEATMPIPESVKVTFKGKMADHMDFRDVVHATQAQMLKQHG HCCEEECCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC DNVFQGRVIEVHIGTLLADQAFTFTDWTAEMKAKASICISNDDTLIGSLELAKSRIQIMI CCCCCCEEEEEEECCHHHCCCEEEECCHHHHCCCEEEEECCCCCEEEHHHHHHCEEEEEE DKGMDNEAGTLQGLIDKADKRIAEIRSGEKPALSPDDSAKYYAEVVVDLDEINEPMIADP ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEEEHHHCCCCEECCC DVNNEDVSKRYTHDTIRPISFYNAEKKVDLGFVGSCMVHKGDMKIVAQMLKNLEKANGKV CCCCHHHHHHHCCCCCCCEEEECCCCCCCCCEECEEEEECCCHHHHHHHHHHHHHCCCEE EFKAPLVVAAPTYNIIDEMKAEGDWEILQKYSGFEFDDLFPKSQARTEYENILYLERPGC EEECCEEEECCCHHHHHHHCCCCCHHHHHHHCCCCHHHCCCCHHHCCCHHCEEEEECCCC NLCMGNQEKAAKGDTVLATSTRLFQGRVVEDAAEKKGESLLASTPVVVLSAILGRTPTIE CEEECCCCCCCCCCEEEEECHHHHCCCHHHHHHHHCCCHHHHCCHHHHHHHHHCCCCCHH EYKTAVEGIDLTKFAPPVEKPAGTRSAHF HHHHHHCCCCCCCCCCCCCCCCCCCCCCC >Mature Secondary Structure SLYTDYLNEIETRKGEGLHPKPIDDGALVAELIAQIKDTGSEHREDSLKFFIYNTLPGT CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCEEEEEEECCCCC TSAAGVKAAFLKEIILGEAEVAEITPEFAFELLSHMKGGPSVEVLLDLALGEDAGIAQQA CCCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHH ADVLKTQVFLYEADTDRLEAAYKAGNAIAREILESYAKAEFFTKLPDVDDEIKVVTYIAA HHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEE EGDISTDLLSPGNQAHSRSDRELHGKCMISEAAQKEIEALKLQHPDKRVMLIAEKGTMGV CCCCCHHHCCCCCHHCCCCCCHHCCCEEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC GSSRMSGVNNVALWTGKQASPYVPFVNYAPVVAGTNGISPIFLTTVGVTGGIGLDLKNWV CCHHHCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCCCCCHHHHH KKVDSDGKAILNNDGNPVLEQKYSVETGTVLKIDTKNKKLLSEDGEELVDVSSAFTPQKV HHHCCCCCEEECCCCCCHHHHEECCCCCCEEEEECCCCHHHHCCCHHHHHHHCCCCCHHH EFMKAGSSYAIVFGKKLQTFAAETLGIEAPLVFAPAKEVSHEGQGLTAVEKIFNRNAVGS HHHHCCCCEEEEECCHHHHHHHHHHCCCCCEEECCHHHHCCCCCCHHHHHHHHCCCCCCC TPGKTLHAGSDVRVKVNIVGSQDTTGLMTSQELEAMAATVISPTVDGAYQSGCHTASVWD CCCCEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHC KKAQANIPRLMKFMNNFGLITARDPKDQYHAMTDVIHKVLNDLTVDDWDIIIGGDSHTRM CHHHCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH SKGVAFGADSGTVALALATGEATMPIPESVKVTFKGKMADHMDFRDVVHATQAQMLKQHG HCCEEECCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC DNVFQGRVIEVHIGTLLADQAFTFTDWTAEMKAKASICISNDDTLIGSLELAKSRIQIMI CCCCCCEEEEEEECCHHHCCCEEEECCHHHHCCCEEEEECCCCCEEEHHHHHHCEEEEEE DKGMDNEAGTLQGLIDKADKRIAEIRSGEKPALSPDDSAKYYAEVVVDLDEINEPMIADP ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEEEHHHCCCCEECCC DVNNEDVSKRYTHDTIRPISFYNAEKKVDLGFVGSCMVHKGDMKIVAQMLKNLEKANGKV CCCCHHHHHHHCCCCCCCEEEECCCCCCCCCEECEEEEECCCHHHHHHHHHHHHHCCCEE EFKAPLVVAAPTYNIIDEMKAEGDWEILQKYSGFEFDDLFPKSQARTEYENILYLERPGC EEECCEEEECCCHHHHHHHCCCCCHHHHHHHCCCCHHHCCCCHHHCCCHHCEEEEECCCC NLCMGNQEKAAKGDTVLATSTRLFQGRVVEDAAEKKGESLLASTPVVVLSAILGRTPTIE CEEECCCCCCCCCCEEEEECHHHHCCCHHHHHHHHCCCHHHHCCHHHHHHHHHCCCCCHH EYKTAVEGIDLTKFAPPVEKPAGTRSAHF HHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]