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The map label for this gene is mazG [H]

Identifier: 99080777

GI number: 99080777

Start: 999002

End: 999844

Strand: Reverse

Name: mazG [H]

Synonym: TM1040_0936

Alternate gene names: 99080777

Gene position: 999844-999002 (Counterclockwise)

Preceding gene: 99080781

Following gene: 99080775

Centisome position: 31.24

GC content: 60.74

Gene sequence:

>843_bases
ATGAGCGACGACAGCTTGATCCACGATGAAACCGCAGGCATCGAACGGCTCCTGGAAATCATGCGCCGCCTGCGCGATCC
AAAGGGCGGCTGCCCTTGGGACATCGAGCAGGATTTTGCCTCCATCGCGCCCTACACCATCGAAGAGGCCTATGAGGTCG
CCGACGCCATCGAGCGTGAGAAATGGGACGAACTGAAGGGTGAGTTGGGCGATCTCTTGTTCCAATCCGTCTTTCACGCA
CAAATGGCTGCCGAAGCCGGGCATTTCACCTTTCAGGATGTGGTCACAACCATGTCCAACAAGATGGTGTCGCGGCACCC
GCATGTTTTTGGCGACGAGAGCCGCGAGAAATCCGCCGAACAGCAAACGCAGGACTGGGAGACCATCAAGGCCGCCGAGC
GCGCCGCAAAGGCCGAGACTGGCGCGCTGGATGGTGTCGCGGTGGGCCTGCCGGCCTTGCTGCGCGCCTACAAGCTGCAA
AAACGCGCGGCGCGGGTGGGGTTTGACTGGCCCTCGGCACAGAATGTGCTCGCCAAGATTGCCGAGGAATCCGCCGAACT
CGTCGAGGCGCGCGACACGCTCACTCAGGATGAGGTCGAAGAAGAATTCGGCGATCTTCTGTTTGTGATGGCCAACCTCG
GTCGCCACCTCGGCGTCGAACCTGAGGCGGCCCTGCGCCGCACCAACGCGAAATTCACCCGCCGCTTCAAGGCGGTAGAG
GCAAAGCTGGCAGAACGGGGCAAGCGCGCCGAAGACAGCAACCTTGAGGAAATGGACGCGCTTTGGAACGCGGTCAAAGC
CGAGGAAAAAGCGCGCAAGGGCCAAGACTCTAAATCAGAGTAA

Upstream 100 bases:

>100_bases
TCTTCGGCAAAATCAGCGATGCGGTTCACGACCGGCATGGGGTGGACTCCTGTCAAATAGTCGGGATGATGCAGCAGACA
CGAAAACGCAGGCAGGTGCA

Downstream 100 bases:

>100_bases
GCCCCGGTTCCTTGGCGATATCTGACAGATCGCGCTCGTAGCGCATTGAGAAGGCGCGCAATTCTCGCGGTGTAAACTCA
GTGAGCCCCATGGGTGTCGC

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSDDSLIHDETAGIERLLEIMRRLRDPKGGCPWDIEQDFASIAPYTIEEAYEVADAIEREKWDELKGELGDLLFQSVFHA
QMAAEAGHFTFQDVVTTMSNKMVSRHPHVFGDESREKSAEQQTQDWETIKAAERAAKAETGALDGVAVGLPALLRAYKLQ
KRAARVGFDWPSAQNVLAKIAEESAELVEARDTLTQDEVEEEFGDLLFVMANLGRHLGVEPEAALRRTNAKFTRRFKAVE
AKLAERGKRAEDSNLEEMDALWNAVKAEEKARKGQDSKSE

Sequences:

>Translated_280_residues
MSDDSLIHDETAGIERLLEIMRRLRDPKGGCPWDIEQDFASIAPYTIEEAYEVADAIEREKWDELKGELGDLLFQSVFHA
QMAAEAGHFTFQDVVTTMSNKMVSRHPHVFGDESREKSAEQQTQDWETIKAAERAAKAETGALDGVAVGLPALLRAYKLQ
KRAARVGFDWPSAQNVLAKIAEESAELVEARDTLTQDEVEEEFGDLLFVMANLGRHLGVEPEAALRRTNAKFTRRFKAVE
AKLAERGKRAEDSNLEEMDALWNAVKAEEKARKGQDSKSE
>Mature_279_residues
SDDSLIHDETAGIERLLEIMRRLRDPKGGCPWDIEQDFASIAPYTIEEAYEVADAIEREKWDELKGELGDLLFQSVFHAQ
MAAEAGHFTFQDVVTTMSNKMVSRHPHVFGDESREKSAEQQTQDWETIKAAERAAKAETGALDGVAVGLPALLRAYKLQK
RAARVGFDWPSAQNVLAKIAEESAELVEARDTLTQDEVEEEFGDLLFVMANLGRHLGVEPEAALRRTNAKFTRRFKAVEA
KLAERGKRAEDSNLEEMDALWNAVKAEEKARKGQDSKSE

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=258, Percent_Identity=55.0387596899225, Blast_Score=276, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 31407; Mature: 31276

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDSLIHDETAGIERLLEIMRRLRDPKGGCPWDIEQDFASIAPYTIEEAYEVADAIERE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHH
KWDELKGELGDLLFQSVFHAQMAAEAGHFTFQDVVTTMSNKMVSRHPHVFGDESREKSAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
QQTQDWETIKAAERAAKAETGALDGVAVGLPALLRAYKLQKRAARVGFDWPSAQNVLAKI
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AEESAELVEARDTLTQDEVEEEFGDLLFVMANLGRHLGVEPEAALRRTNAKFTRRFKAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AKLAERGKRAEDSNLEEMDALWNAVKAEEKARKGQDSKSE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDDSLIHDETAGIERLLEIMRRLRDPKGGCPWDIEQDFASIAPYTIEEAYEVADAIERE
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHH
KWDELKGELGDLLFQSVFHAQMAAEAGHFTFQDVVTTMSNKMVSRHPHVFGDESREKSAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
QQTQDWETIKAAERAAKAETGALDGVAVGLPALLRAYKLQKRAARVGFDWPSAQNVLAKI
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
AEESAELVEARDTLTQDEVEEEFGDLLFVMANLGRHLGVEPEAALRRTNAKFTRRFKAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AKLAERGKRAEDSNLEEMDALWNAVKAEEKARKGQDSKSE
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]