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The map label for this gene is tyrS

Identifier: 99080764

GI number: 99080764

Start: 984889

End: 986142

Strand: Reverse

Name: tyrS

Synonym: TM1040_0923

Alternate gene names: 99080764

Gene position: 986142-984889 (Counterclockwise)

Preceding gene: 99080766

Following gene: 99080763

Centisome position: 30.81

GC content: 59.73

Gene sequence:

>1254_bases
ATGACCTACCATCCGAAATCGGACTTCATCGCCATCATGATGGAACGCGGATTCCTGGCGGATTGCACCGATTATCAGGG
TCTCGATGAGGCACTGGTGAGCGGTACACAGACGGCATATATCGGCTATGACGCCACGGCGCAGTCGCTGCACGTGGGGC
ACCTTCTGAACATCATGATGCTGCGCTGGCTGCAAAAAACCGGCCACCGCCCGATCACCCTGATGGGGGGCGGCACCACC
AAGGTGGGCGATCCTTCGTTCCGCTCGGATGAACGCCCGCTGCTGGGGCCGGAGCAGATCGACGCCAACATTGCAGGCAT
GCAGAAGGTGTTTGCTAAATACCTCGACTACTCTGACGCCAAAAATGGCGCCATGATGCTCAACAACGCCGAATGGCTGG
ATGGGCTCAATTACCTTGAGTTCCTGCGCGACATCGGGCGGCATTTCTCGGTGAACCGGATGCTGTCCTTCGAGAGCGTG
AAATCACGCCTTGATCGCGAACAGTCGCTGTCCTTCCTCGAGTTCAACTACATGATCCTGCAGGCCTATGATTTCCTGGA
GCTGAACCGCCGCTATGGCTGTGTGTTGCAGATGGGCGGCTCGGATCAATGGGGCAATATCGTCAACGGGATCGACCTGA
CGCGCCGGGTGTTGGAGAATGAGATCTATGGCCTGACCTCGCCGCTGCTGACAACCTCGGACGGGCGCAAGATGGGCAAA
TCGCAAGGCGGCGCGATCTGGCTCAATGACGAGATGCTGAGCCCCTATGAGTTCTGGCAATTCTGGCGCAACACCACCGA
TGCGGATGTGGGGCGGTTCCTGAAGCTCTATACCGAGCTGCCGGTTGAAGAATGCGACCGTCTGGGCGCGCTTCAGGGGT
CTGAGATCAACGAGGGCAAGATCATCCTTGCCAATGAGGTCACGACGCTCCTGCATGGGGCCGAGGCGGCGCGCGCTGCC
GAGGCGACAGCGCGCGAAGTGTTCGAAAAGGGCGGTGTGGGCGATGACCTGCCGACCCTGTCGCTGTCGCCCGCGGAGCT
TGGCGACGGGATGTCGATCGTACAGTTGATCGTGAAATCCGGGCTCGCAAAATCCGGCAAGGAAGCAAAGCGCCTGATCT
CTGAGAATGGCGCCAAGCTTGACGATGCGCCGCTGACGGATGCCGGTCTGATGATCGACGCCGCTGCGCTGGCCTCCCCG
ATCAAACTGTCGGCAGGTAAAAAGCGTCACGCGCTTGTGCAGCTCGAGGGCTGA

Upstream 100 bases:

>100_bases
CGGCACATGATACACCGCACATTTTGTCCCGAAAACAGGTTCCCACCGGTGTCGATGCGATTTATACAGCCGCTTGCATA
TTCAAAAGCCGAGGCATGCT

Downstream 100 bases:

>100_bases
TGGAAACAGAGGACGAGAGGGGCTTTGCCCCTCTTGGCCCCTGGCCAATTCACCCCAGGATATTTTGGGTTAGAAGAAGC
CAGCGGTCTGTCCTGTTGGG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTYHPKSDFIAIMMERGFLADCTDYQGLDEALVSGTQTAYIGYDATAQSLHVGHLLNIMMLRWLQKTGHRPITLMGGGTT
KVGDPSFRSDERPLLGPEQIDANIAGMQKVFAKYLDYSDAKNGAMMLNNAEWLDGLNYLEFLRDIGRHFSVNRMLSFESV
KSRLDREQSLSFLEFNYMILQAYDFLELNRRYGCVLQMGGSDQWGNIVNGIDLTRRVLENEIYGLTSPLLTTSDGRKMGK
SQGGAIWLNDEMLSPYEFWQFWRNTTDADVGRFLKLYTELPVEECDRLGALQGSEINEGKIILANEVTTLLHGAEAARAA
EATAREVFEKGGVGDDLPTLSLSPAELGDGMSIVQLIVKSGLAKSGKEAKRLISENGAKLDDAPLTDAGLMIDAAALASP
IKLSAGKKRHALVQLEG

Sequences:

>Translated_417_residues
MTYHPKSDFIAIMMERGFLADCTDYQGLDEALVSGTQTAYIGYDATAQSLHVGHLLNIMMLRWLQKTGHRPITLMGGGTT
KVGDPSFRSDERPLLGPEQIDANIAGMQKVFAKYLDYSDAKNGAMMLNNAEWLDGLNYLEFLRDIGRHFSVNRMLSFESV
KSRLDREQSLSFLEFNYMILQAYDFLELNRRYGCVLQMGGSDQWGNIVNGIDLTRRVLENEIYGLTSPLLTTSDGRKMGK
SQGGAIWLNDEMLSPYEFWQFWRNTTDADVGRFLKLYTELPVEECDRLGALQGSEINEGKIILANEVTTLLHGAEAARAA
EATAREVFEKGGVGDDLPTLSLSPAELGDGMSIVQLIVKSGLAKSGKEAKRLISENGAKLDDAPLTDAGLMIDAAALASP
IKLSAGKKRHALVQLEG
>Mature_416_residues
TYHPKSDFIAIMMERGFLADCTDYQGLDEALVSGTQTAYIGYDATAQSLHVGHLLNIMMLRWLQKTGHRPITLMGGGTTK
VGDPSFRSDERPLLGPEQIDANIAGMQKVFAKYLDYSDAKNGAMMLNNAEWLDGLNYLEFLRDIGRHFSVNRMLSFESVK
SRLDREQSLSFLEFNYMILQAYDFLELNRRYGCVLQMGGSDQWGNIVNGIDLTRRVLENEIYGLTSPLLTTSDGRKMGKS
QGGAIWLNDEMLSPYEFWQFWRNTTDADVGRFLKLYTELPVEECDRLGALQGSEINEGKIILANEVTTLLHGAEAARAAE
ATAREVFEKGGVGDDLPTLSLSPAELGDGMSIVQLIVKSGLAKSGKEAKRLISENGAKLDDAPLTDAGLMIDAAALASPI
KLSAGKKRHALVQLEG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=379, Percent_Identity=36.6754617414248, Blast_Score=224, Evalue=2e-58,
Organism=Escherichia coli, GI1787925, Length=422, Percent_Identity=41.7061611374408, Blast_Score=335, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI25145802, Length=313, Percent_Identity=34.5047923322684, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI25145805, Length=226, Percent_Identity=34.070796460177, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6325160, Length=337, Percent_Identity=36.4985163204748, Blast_Score=217, Evalue=2e-57,
Organism=Drosophila melanogaster, GI20130383, Length=394, Percent_Identity=37.3096446700508, Blast_Score=249, Evalue=3e-66,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_SILST (Q1GI60)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612918.1
- ProteinModelPortal:   Q1GI60
- SMR:   Q1GI60
- STRING:   Q1GI60
- GeneID:   4077551
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0923
- NMPDR:   fig|292414.1.peg.3003
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   WINQDDR
- PhylomeDB:   Q1GI60
- ProtClustDB:   PRK05912
- BioCyc:   SSP292414:TM1040_0923-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 46008; Mature: 45877

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 40-40 BINDING 177-177 BINDING 181-181 BINDING 240-240

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYHPKSDFIAIMMERGFLADCTDYQGLDEALVSGTQTAYIGYDATAQSLHVGHLLNIMM
CCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHH
LRWLQKTGHRPITLMGGGTTKVGDPSFRSDERPLLGPEQIDANIAGMQKVFAKYLDYSDA
HHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCC
KNGAMMLNNAEWLDGLNYLEFLRDIGRHFSVNRMLSFESVKSRLDREQSLSFLEFNYMIL
CCCEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHCCHHHHHHHHHH
QAYDFLELNRRYGCVLQMGGSDQWGNIVNGIDLTRRVLENEIYGLTSPLLTTSDGRKMGK
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHC
SQGGAIWLNDEMLSPYEFWQFWRNTTDADVGRFLKLYTELPVEECDRLGALQGSEINEGK
CCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCE
IILANEVTTLLHGAEAARAAEATAREVFEKGGVGDDLPTLSLSPAELGDGMSIVQLIVKS
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
GLAKSGKEAKRLISENGAKLDDAPLTDAGLMIDAAALASPIKLSAGKKRHALVQLEG
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCEEEEEECC
>Mature Secondary Structure 
TYHPKSDFIAIMMERGFLADCTDYQGLDEALVSGTQTAYIGYDATAQSLHVGHLLNIMM
CCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHH
LRWLQKTGHRPITLMGGGTTKVGDPSFRSDERPLLGPEQIDANIAGMQKVFAKYLDYSDA
HHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCC
KNGAMMLNNAEWLDGLNYLEFLRDIGRHFSVNRMLSFESVKSRLDREQSLSFLEFNYMIL
CCCEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHCCHHHHHHHHHH
QAYDFLELNRRYGCVLQMGGSDQWGNIVNGIDLTRRVLENEIYGLTSPLLTTSDGRKMGK
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHC
SQGGAIWLNDEMLSPYEFWQFWRNTTDADVGRFLKLYTELPVEECDRLGALQGSEINEGK
CCCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCE
IILANEVTTLLHGAEAARAAEATAREVFEKGGVGDDLPTLSLSPAELGDGMSIVQLIVKS
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
GLAKSGKEAKRLISENGAKLDDAPLTDAGLMIDAAALASPIKLSAGKKRHALVQLEG
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA