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The map label for this gene is glmU

Identifier: 99080569

GI number: 99080569

Start: 785636

End: 786985

Strand: Reverse

Name: glmU

Synonym: TM1040_0728

Alternate gene names: 99080569

Gene position: 786985-785636 (Counterclockwise)

Preceding gene: 99080571

Following gene: 99080568

Centisome position: 24.59

GC content: 62.52

Gene sequence:

>1350_bases
ATGAGCACTGCCCTCGTCATCCTCGCCGCAGGCAAAGGCACCAGGATGAACTCCGATTTGCCCAAAGTCCTGCATCAGAT
CGCACATGCGCCGATGCTGGAACATGCGATGCGCGCCGGGGGGGCGCTTGACCCCGAGCGCACGGTGGTTGTGGCAGGCC
ACGAGGCCGAGATGGTGCGCGCGGCCACCGCAGAGATCGCCCCTGAAGCGACAGTGGTGCTGCAGGAAGAGCAGCTCGGC
ACCGGCCACGCGGTACTTCAAGCGCGCGCGGCACTCGAGGGATTTCGCGGCGATGTCGTGGTGCTCTATGGCGATACGCC
TTTTGTGTCGGCCGAGACGCTGGAACGCATGATCGAAGCGCGCAGCCGCGCCGATCTAGTGATCCTCGGCTTTGAAGCCG
CCGATCCTGCGCGCTACGGCCGGTTGATCATGCAGGGCGAAAGCCTTGAGAAAATCGTCGAGTTCAAGGACGCAAGCGAC
GCCGAGCGCGCGATTACATTCTGCAACTCGGGCCTCATGGCGTGCAACGCCGAGGTGATGTTTGGCCTGCTTGATCAAGT
GGGCAACGACAATGCCTCTGGCGAATACTACCTGACCGATCTTGTCGAACTCGCGCGGGCCGAGGGGCTGAGCGTCACGG
CCGTGTCCTGCCCCGAAGCGGAAACGCTCGGCATCAATTCCCGCGCGGACCTCGCGGCCGCGGAGGCGGTGTTTCAGGCG
CATGCGCGGGCTGAGTTGTTGGACATCGGCGTCACGCTGACGGCTCCTGAGACCGTCCATCTGGCCTTTGACACCATCAT
TGGTCGTGACACGGTGATTGAACCCAATGTGGTCTTTGGTCCCGGTGTCACCGTTGAGAGCGGCGCTTTGATCCGGGCGT
TTTCGCACCTTGAGGGCTGCCATGTGTCGCGTGGCGCCAAGGTCGGCCCCTACGCCCGCCTGCGCCCCGGCGCGGAGCTG
GCCGAGGACACCCATGTGGGCAACTTCGTTGAAATCAAGAACGCTGAGATCGCCGCAGGCGCCAAGGTGAACCACCTGAC
CTATATTGGCGATGCCTCTGTGGGTGAGGCGACGAATATCGGAGCGGGCACAATCACCTGCAACTACGATGGCGTCATGA
AGCATCGCACCGAAATCGGCGCGCGCGCCTTTATCGGATCAAACACGTGTTTGGTCGCCCCGGTGACCGTTGGCGATGAG
GCGATGACGGCAACAGGTGCTGTCATCACCAAGGATGTCGCTGATGGAGATCTGGCGATTGCGCGCGTCCAGCAGACGAA
CAAACCAGGCCGCGCACGCAAGCTGATGGATATGCTGCGCGCCAAGAAAGCCGCAAAGGCCAAAGGGTAA

Upstream 100 bases:

>100_bases
GATGTTGCAAGTCGCAACTGAAGTTTATGGCAGCGCATCTTGCGCATCCTCTGTTAGCGGATAGAACGGGGGCAACAAAA
ACTCAAAGGGAAAACACCCC

Downstream 100 bases:

>100_bases
TCACATGTGTGGAATTATCGGAGTTCTGGGCAACCATGAAGTCTCGCCCATTTTGGTCGAAGCGCTGAAACGGCTGGAAT
ATCGCGGATATGACAGTGCA

Product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSTALVILAAGKGTRMNSDLPKVLHQIAHAPMLEHAMRAGGALDPERTVVVAGHEAEMVRAATAEIAPEATVVLQEEQLG
TGHAVLQARAALEGFRGDVVVLYGDTPFVSAETLERMIEARSRADLVILGFEAADPARYGRLIMQGESLEKIVEFKDASD
AERAITFCNSGLMACNAEVMFGLLDQVGNDNASGEYYLTDLVELARAEGLSVTAVSCPEAETLGINSRADLAAAEAVFQA
HARAELLDIGVTLTAPETVHLAFDTIIGRDTVIEPNVVFGPGVTVESGALIRAFSHLEGCHVSRGAKVGPYARLRPGAEL
AEDTHVGNFVEIKNAEIAAGAKVNHLTYIGDASVGEATNIGAGTITCNYDGVMKHRTEIGARAFIGSNTCLVAPVTVGDE
AMTATGAVITKDVADGDLAIARVQQTNKPGRARKLMDMLRAKKAAKAKG

Sequences:

>Translated_449_residues
MSTALVILAAGKGTRMNSDLPKVLHQIAHAPMLEHAMRAGGALDPERTVVVAGHEAEMVRAATAEIAPEATVVLQEEQLG
TGHAVLQARAALEGFRGDVVVLYGDTPFVSAETLERMIEARSRADLVILGFEAADPARYGRLIMQGESLEKIVEFKDASD
AERAITFCNSGLMACNAEVMFGLLDQVGNDNASGEYYLTDLVELARAEGLSVTAVSCPEAETLGINSRADLAAAEAVFQA
HARAELLDIGVTLTAPETVHLAFDTIIGRDTVIEPNVVFGPGVTVESGALIRAFSHLEGCHVSRGAKVGPYARLRPGAEL
AEDTHVGNFVEIKNAEIAAGAKVNHLTYIGDASVGEATNIGAGTITCNYDGVMKHRTEIGARAFIGSNTCLVAPVTVGDE
AMTATGAVITKDVADGDLAIARVQQTNKPGRARKLMDMLRAKKAAKAKG
>Mature_448_residues
STALVILAAGKGTRMNSDLPKVLHQIAHAPMLEHAMRAGGALDPERTVVVAGHEAEMVRAATAEIAPEATVVLQEEQLGT
GHAVLQARAALEGFRGDVVVLYGDTPFVSAETLERMIEARSRADLVILGFEAADPARYGRLIMQGESLEKIVEFKDASDA
ERAITFCNSGLMACNAEVMFGLLDQVGNDNASGEYYLTDLVELARAEGLSVTAVSCPEAETLGINSRADLAAAEAVFQAH
ARAELLDIGVTLTAPETVHLAFDTIIGRDTVIEPNVVFGPGVTVESGALIRAFSHLEGCHVSRGAKVGPYARLRPGAELA
EDTHVGNFVEIKNAEIAAGAKVNHLTYIGDASVGEATNIGAGTITCNYDGVMKHRTEIGARAFIGSNTCLVAPVTVGDEA
MTATGAVITKDVADGDLAIARVQQTNKPGRARKLMDMLRAKKAAKAKG

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=449, Percent_Identity=42.7616926503341, Blast_Score=336, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_SILST (Q1GIQ5)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612723.1
- ProteinModelPortal:   Q1GIQ5
- SMR:   Q1GIQ5
- STRING:   Q1GIQ5
- GeneID:   4076098
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0728
- NMPDR:   fig|292414.1.peg.2833
- eggNOG:   COG1207
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- PhylomeDB:   Q1GIQ5
- ProtClustDB:   PRK14353
- BioCyc:   SSP292414:TM1040_0728-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 47193; Mature: 47062

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 345-345 BINDING 75-75 BINDING 140-140 BINDING 154-154 BINDING 169-169 BINDING 369-369 BINDING 387-387 BINDING 422-422

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTALVILAAGKGTRMNSDLPKVLHQIAHAPMLEHAMRAGGALDPERTVVVAGHEAEMVR
CCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHH
AATAEIAPEATVVLQEEQLGTGHAVLQARAALEGFRGDVVVLYGDTPFVSAETLERMIEA
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
RSRADLVILGFEAADPARYGRLIMQGESLEKIVEFKDASDAERAITFCNSGLMACNAEVM
HCCCCEEEEEECCCCHHHHCEEEECCCHHHHHHHHCCCCCHHHHHHHHCCCCEEECHHHH
FGLLDQVGNDNASGEYYLTDLVELARAEGLSVTAVSCPEAETLGINSRADLAAAEAVFQA
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCCHHHHHHHHHHH
HARAELLDIGVTLTAPETVHLAFDTIIGRDTVIEPNVVFGPGVTVESGALIRAFSHLEGC
HHHHEEEEEEEEEECCCEEEEEEHHHCCCCCEECCCEEECCCCEECCCHHHHHHHHCCCC
HVSRGAKVGPYARLRPGAELAEDTHVGNFVEIKNAEIAAGAKVNHLTYIGDASVGEATNI
CCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCEEECCCEEEEEEEECCCCCCCCCCC
GAGTITCNYDGVMKHRTEIGARAFIGSNTCLVAPVTVGDEAMTATGAVITKDVADGDLAI
CCEEEEECCCCHHHHHHHHCCEEEECCCCEEEEEEEECCCHHCCCCCEEEECCCCCCEEE
ARVQQTNKPGRARKLMDMLRAKKAAKAKG
EEEHHCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STALVILAAGKGTRMNSDLPKVLHQIAHAPMLEHAMRAGGALDPERTVVVAGHEAEMVR
CCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHH
AATAEIAPEATVVLQEEQLGTGHAVLQARAALEGFRGDVVVLYGDTPFVSAETLERMIEA
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHH
RSRADLVILGFEAADPARYGRLIMQGESLEKIVEFKDASDAERAITFCNSGLMACNAEVM
HCCCCEEEEEECCCCHHHHCEEEECCCHHHHHHHHCCCCCHHHHHHHHCCCCEEECHHHH
FGLLDQVGNDNASGEYYLTDLVELARAEGLSVTAVSCPEAETLGINSRADLAAAEAVFQA
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCCHHHHHHHHHHH
HARAELLDIGVTLTAPETVHLAFDTIIGRDTVIEPNVVFGPGVTVESGALIRAFSHLEGC
HHHHEEEEEEEEEECCCEEEEEEHHHCCCCCEECCCEEECCCCEECCCHHHHHHHHCCCC
HVSRGAKVGPYARLRPGAELAEDTHVGNFVEIKNAEIAAGAKVNHLTYIGDASVGEATNI
CCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCEEECCCEEEEEEEECCCCCCCCCCC
GAGTITCNYDGVMKHRTEIGARAFIGSNTCLVAPVTVGDEAMTATGAVITKDVADGDLAI
CCEEEEECCCCHHHHHHHHCCEEEECCCCEEEEEEEECCCHHCCCCCEEEECCCCCCEEE
ARVQQTNKPGRARKLMDMLRAKKAAKAKG
EEEHHCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA