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The map label for this gene is lytF [H]

Identifier: 94985654

GI number: 94985654

Start: 1654046

End: 1654672

Strand: Reverse

Name: lytF [H]

Synonym: Dgeo_1554

Alternate gene names: 94985654

Gene position: 1654672-1654046 (Counterclockwise)

Preceding gene: 94985658

Following gene: 94985651

Centisome position: 67.07

GC content: 63.48

Gene sequence:

>627_bases
ATGAAAGCGTTCCAAACCCTCCTGGTCGCGACCGCGCTTTGCAGTGGTGGTGCCTTCGCCGCCTCCTACACTGTCAAGCC
CGGAGATACGCTCTCCAGCATTGCCAGCCGCTACAAGCTGGAACCGGCGCAGCTGATGCGGCTCAACGGACTCAGCAGCA
CGACCATTCAGGTTGGTCAGAAGCTGAATGTGGGTGGAGCCCAAGCACAGACGGCGACAAAGACTCCGGCGCCAGTCGCT
CGGACCTCCACCTCCGGCGGCGCCTTTATCCGCACGGCCGCCACCCGTTTTCTGGGCATTCGGTACGTGCTGGGCGGCAC
AGGAAACGGCGGAATCGACTGCTCGGGCTTTACCATGCGCGTCTTTCAGCAGCTTGGAATCAAGCTCCCCCACAGCGCAG
CCGGTCAGTGGCGCATGGGGTACGCCGTCAACAGCCGCAACCTGCAACCCGGCGATCTGGTGTTCTTCAATACGCTGGGA
CGCGGCGTCAGCCATGTGGGCGTGTATGTCGGGGACGGCCTGATGGCGAATGCGAACAGCTATCAGGGCCGCACCGTCAT
CGAGCCGCTCTTCAGTAACCCCTACTGGGCCAGCCGGTACCTGGGGGCGCGCCGCGTCCTGAGCTAA

Upstream 100 bases:

>100_bases
TGTGCCGGGCCGCATAGAGGACGTATAGGCGCATAAAAACCTGGTCTTCTTCCAAGGATTAAGGAAAAAAGAATAAAGAC
GGGTTAAACTTCGCGGGTCG

Downstream 100 bases:

>100_bases
AGCTCGCCTCCACCCTCATCGACCGCACGCCCCTGTTCTGCCGGCGGGGGCGTGTGCCTTTGGCTCAGCACAACAAGCAC
AAGAGGGGAAGCCTTTCTGC

Product: NLP/P60

Products: NA

Alternate protein names: Cell wall-associated polypeptide CWBP49; Gamma-D-glutamate-meso-diaminopimelate muropeptidase lytF [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKAFQTLLVATALCSGGAFAASYTVKPGDTLSSIASRYKLEPAQLMRLNGLSSTTIQVGQKLNVGGAQAQTATKTPAPVA
RTSTSGGAFIRTAATRFLGIRYVLGGTGNGGIDCSGFTMRVFQQLGIKLPHSAAGQWRMGYAVNSRNLQPGDLVFFNTLG
RGVSHVGVYVGDGLMANANSYQGRTVIEPLFSNPYWASRYLGARRVLS

Sequences:

>Translated_208_residues
MKAFQTLLVATALCSGGAFAASYTVKPGDTLSSIASRYKLEPAQLMRLNGLSSTTIQVGQKLNVGGAQAQTATKTPAPVA
RTSTSGGAFIRTAATRFLGIRYVLGGTGNGGIDCSGFTMRVFQQLGIKLPHSAAGQWRMGYAVNSRNLQPGDLVFFNTLG
RGVSHVGVYVGDGLMANANSYQGRTVIEPLFSNPYWASRYLGARRVLS
>Mature_208_residues
MKAFQTLLVATALCSGGAFAASYTVKPGDTLSSIASRYKLEPAQLMRLNGLSSTTIQVGQKLNVGGAQAQTATKTPAPVA
RTSTSGGAFIRTAATRFLGIRYVLGGTGNGGIDCSGFTMRVFQQLGIKLPHSAAGQWRMGYAVNSRNLQPGDLVFFNTLG
RGVSHVGVYVGDGLMANANSYQGRTVIEPLFSNPYWASRYLGARRVLS

Specific function: Cleaves gamma-D-glutamate-meso-diaminopimelate bonds. Cell wall hydrolase involved in cell autolysis [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted, cell wall. Note=LysM repeats are thought to be involved in peptidoglycan binding [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1788501, Length=111, Percent_Identity=46.8468468468468, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1786421, Length=116, Percent_Identity=37.0689655172414, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787944, Length=116, Percent_Identity=37.0689655172414, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1788001, Length=139, Percent_Identity=33.8129496402878, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 21961; Mature: 21961

Theoretical pI: Translated: 10.97; Mature: 10.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFQTLLVATALCSGGAFAASYTVKPGDTLSSIASRYKLEPAQLMRLNGLSSTTIQVGQ
CHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHCC
KLNVGGAQAQTATKTPAPVARTSTSGGAFIRTAATRFLGIRYVLGGTGNGGIDCSGFTMR
EECCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHEEEEEEECCCCCCEECCCHHHH
VFQQLGIKLPHSAAGQWRMGYAVNSRNLQPGDLVFFNTLGRGVSHVGVYVGDGLMANANS
HHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHCCCHHHEEEEECCCEEECCCC
YQGRTVIEPLFSNPYWASRYLGARRVLS
CCCCEEEHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKAFQTLLVATALCSGGAFAASYTVKPGDTLSSIASRYKLEPAQLMRLNGLSSTTIQVGQ
CHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHCC
KLNVGGAQAQTATKTPAPVARTSTSGGAFIRTAATRFLGIRYVLGGTGNGGIDCSGFTMR
EECCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHEEEEEEECCCCCCEECCCHHHH
VFQQLGIKLPHSAAGQWRMGYAVNSRNLQPGDLVFFNTLGRGVSHVGVYVGDGLMANANS
HHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHCCCHHHEEEEECCCEEECCCC
YQGRTVIEPLFSNPYWASRYLGARRVLS
CCCCEEEHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 10206711 [H]