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The map label for this gene is dapE [H]

Identifier: 94985647

GI number: 94985647

Start: 1647945

End: 1649312

Strand: Reverse

Name: dapE [H]

Synonym: Dgeo_1547

Alternate gene names: 94985647

Gene position: 1649312-1647945 (Counterclockwise)

Preceding gene: 94985651

Following gene: 94985646

Centisome position: 66.85

GC content: 67.84

Gene sequence:

>1368_bases
ATGACCCAGTCTCAGAATGCCGATCTGATCGCCTGGCAAGACCGCGCGGGGGCCGAGCAGGAACTCTTTGAGCTGCTGCG
GATTCCCAGCGTGAGTGCCGACCCTGCCCACGTCGGGGACATGGCGCGGGCGGCGGAGTTTTTGCGCAGCAAGCTGCAAG
CGCTTGGGTTGACGGCGCGGGTGGACCCAACCGGGGGGCATCCGGTCGTGTATGCCGAGCGGCTGGAGGCACCCGGCAAG
CCCACCGTGCTGATCTACGGACACTACGACGTCCAGCCTGAGGCGCCGCTGGACGAATGGGAGACGCCCCCCTTCGAGCC
GACCGTGCGCGAAGGCCGCATCTATGCGCGTGGTGCTACCGACGACAAGGGGCAGGCATATGCCCATGTGCGCGGGGTGG
AACTGCTCCTGCGGGCCGGGCCGCTCCCAGTGAACGTGAAGTTTCTGCTGGAGGGCGAAGAGGAGGTGGGCAGCCCCAAC
TTGGAAGCGTACCTGCGCGACCATGCCGACGAGCTGCGAGCAGACGTGATCGTGATCTCCGACGGCAGCCGCTTTGCGCC
GGACGTGCCCACCGTTACCTACGGCCTGCGCGGATTGAGCTACGTCGAGATTCACGTGCAGGGCGCGAGCCGCGACCTCC
ACTCCGGCTCCTACGGGGGAGCCGCGCCCAACCCCATCAACGCGCTGTGCGAGATCATCGCCCGCCTCAAGGACGACCAG
GGCCGCGTCACCATCCCCGGTTTCTATGACGGGGTGGAGGAACTGACCCCGGAAGAGCGCGAGATGTGGGCGGGGCTGCC
GCACAGTGACGAAGCGTTCGCCGCCTCCATCGGGGTGTCTGACCTGCCGGGCGAGCAGGGGTACAGCACGCTCGAGCGCC
TCTGGGCGCGGCCCACCCTCGACGTGAACGGCATCTGGGGTGGCTACCAGGGCGAGGGCAGCAAGACCGTGATTCCGGCG
AAGGCCGGGGCCAAGGTGTCCATGCGGCTGGTGCCCGGTCAGGACCCCGAGCGCATCACTCGCCTCGTGCAGGCGTACGT
GCCCACCCTCGCTCCCCGCGGCGTCCAGGTGGAGGTCAAGGCGCTGCACGGCGGCCAACCCGTCAAGGTGGACCTCGACT
CGCCCTTTGTCAAGGCTGCTGACCGCGCCCTGCGGCGGGTGTATGGCAAGCCCGCCGCCTTTGCCCGCACGGGGGGCAGT
ATTCCGATCGTCGCCGCCTTCCGTCAGATTCTAGGGGCGCCTGTCCTGCTGGTGGACTTTGGCCTCAACGAGGACGCGCC
GCACTCCCCCAACGAGAGCTTCGCCCTGGAGGACTACCACAACGGCATCCTGACGAGCGCCTTCCTGCTTCAGGAGATCG
GGAAGTGA

Upstream 100 bases:

>100_bases
TGAACGTGTCGAAAGGTGAGGTTGGTAGCAGGGGCAGCCCGAGCGCTATCTCCCTACCGGGCGCGCCCTCTTCACCCGTC
CACCTCGGTATCATCCCGCC

Downstream 100 bases:

>100_bases
AGTTGGAGGGCGAGGGGCAGTCTTGCTCCATTCACCCCTCCTCGCTTTTCGCGGACGGCGCGGAGCGCCCATGATCCTCG
GCTTCCTCGCCTCTCATGGC

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTQSQNADLIAWQDRAGAEQELFELLRIPSVSADPAHVGDMARAAEFLRSKLQALGLTARVDPTGGHPVVYAERLEAPGK
PTVLIYGHYDVQPEAPLDEWETPPFEPTVREGRIYARGATDDKGQAYAHVRGVELLLRAGPLPVNVKFLLEGEEEVGSPN
LEAYLRDHADELRADVIVISDGSRFAPDVPTVTYGLRGLSYVEIHVQGASRDLHSGSYGGAAPNPINALCEIIARLKDDQ
GRVTIPGFYDGVEELTPEEREMWAGLPHSDEAFAASIGVSDLPGEQGYSTLERLWARPTLDVNGIWGGYQGEGSKTVIPA
KAGAKVSMRLVPGQDPERITRLVQAYVPTLAPRGVQVEVKALHGGQPVKVDLDSPFVKAADRALRRVYGKPAAFARTGGS
IPIVAAFRQILGAPVLLVDFGLNEDAPHSPNESFALEDYHNGILTSAFLLQEIGK

Sequences:

>Translated_455_residues
MTQSQNADLIAWQDRAGAEQELFELLRIPSVSADPAHVGDMARAAEFLRSKLQALGLTARVDPTGGHPVVYAERLEAPGK
PTVLIYGHYDVQPEAPLDEWETPPFEPTVREGRIYARGATDDKGQAYAHVRGVELLLRAGPLPVNVKFLLEGEEEVGSPN
LEAYLRDHADELRADVIVISDGSRFAPDVPTVTYGLRGLSYVEIHVQGASRDLHSGSYGGAAPNPINALCEIIARLKDDQ
GRVTIPGFYDGVEELTPEEREMWAGLPHSDEAFAASIGVSDLPGEQGYSTLERLWARPTLDVNGIWGGYQGEGSKTVIPA
KAGAKVSMRLVPGQDPERITRLVQAYVPTLAPRGVQVEVKALHGGQPVKVDLDSPFVKAADRALRRVYGKPAAFARTGGS
IPIVAAFRQILGAPVLLVDFGLNEDAPHSPNESFALEDYHNGILTSAFLLQEIGK
>Mature_454_residues
TQSQNADLIAWQDRAGAEQELFELLRIPSVSADPAHVGDMARAAEFLRSKLQALGLTARVDPTGGHPVVYAERLEAPGKP
TVLIYGHYDVQPEAPLDEWETPPFEPTVREGRIYARGATDDKGQAYAHVRGVELLLRAGPLPVNVKFLLEGEEEVGSPNL
EAYLRDHADELRADVIVISDGSRFAPDVPTVTYGLRGLSYVEIHVQGASRDLHSGSYGGAAPNPINALCEIIARLKDDQG
RVTIPGFYDGVEELTPEEREMWAGLPHSDEAFAASIGVSDLPGEQGYSTLERLWARPTLDVNGIWGGYQGEGSKTVIPAK
AGAKVSMRLVPGQDPERITRLVQAYVPTLAPRGVQVEVKALHGGQPVKVDLDSPFVKAADRALRRVYGKPAAFARTGGSI
PIVAAFRQILGAPVLLVDFGLNEDAPHSPNESFALEDYHNGILTSAFLLQEIGK

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=455, Percent_Identity=34.0659340659341, Blast_Score=255, Evalue=8e-68,
Organism=Homo sapiens, GI21071039, Length=440, Percent_Identity=33.6363636363636, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI271398277, Length=321, Percent_Identity=34.8909657320872, Blast_Score=193, Evalue=3e-49,
Organism=Escherichia coli, GI1788816, Length=234, Percent_Identity=29.0598290598291, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193211059, Length=456, Percent_Identity=35.5263157894737, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17563146, Length=464, Percent_Identity=35.1293103448276, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6321159, Length=453, Percent_Identity=33.3333333333333, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6319758, Length=466, Percent_Identity=27.4678111587983, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24585880, Length=462, Percent_Identity=35.4978354978355, Blast_Score=259, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 49195; Mature: 49064

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSQNADLIAWQDRAGAEQELFELLRIPSVSADPAHVGDMARAAEFLRSKLQALGLTAR
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
VDPTGGHPVVYAERLEAPGKPTVLIYGHYDVQPEAPLDEWETPPFEPTVREGRIYARGAT
ECCCCCCCEEEHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
DDKGQAYAHVRGVELLLRAGPLPVNVKFLLEGEEEVGSPNLEAYLRDHADELRADVIVIS
CCCCCEEEHHHHHHHHHHCCCCCEEEEEEEECHHHHCCCCHHHHHHHHHHHHCCCEEEEE
DGSRFAPDVPTVTYGLRGLSYVEIHVQGASRDLHSGSYGGAAPNPINALCEIIARLKDDQ
CCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
GRVTIPGFYDGVEELTPEEREMWAGLPHSDEAFAASIGVSDLPGEQGYSTLERLWARPTL
CCEECCHHHCCHHHHCCCHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHCCCCC
DVNGIWGGYQGEGSKTVIPAKAGAKVSMRLVPGQDPERITRLVQAYVPTLAPRGVQVEVK
CCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
ALHGGQPVKVDLDSPFVKAADRALRRVYGKPAAFARTGGSIPIVAAFRQILGAPVLLVDF
EECCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHCCCEEEEEE
GLNEDAPHSPNESFALEDYHNGILTSAFLLQEIGK
CCCCCCCCCCCCCEEHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQSQNADLIAWQDRAGAEQELFELLRIPSVSADPAHVGDMARAAEFLRSKLQALGLTAR
CCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
VDPTGGHPVVYAERLEAPGKPTVLIYGHYDVQPEAPLDEWETPPFEPTVREGRIYARGAT
ECCCCCCCEEEHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
DDKGQAYAHVRGVELLLRAGPLPVNVKFLLEGEEEVGSPNLEAYLRDHADELRADVIVIS
CCCCCEEEHHHHHHHHHHCCCCCEEEEEEEECHHHHCCCCHHHHHHHHHHHHCCCEEEEE
DGSRFAPDVPTVTYGLRGLSYVEIHVQGASRDLHSGSYGGAAPNPINALCEIIARLKDDQ
CCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
GRVTIPGFYDGVEELTPEEREMWAGLPHSDEAFAASIGVSDLPGEQGYSTLERLWARPTL
CCEECCHHHCCHHHHCCCHHHHHCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHCCCCC
DVNGIWGGYQGEGSKTVIPAKAGAKVSMRLVPGQDPERITRLVQAYVPTLAPRGVQVEVK
CCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
ALHGGQPVKVDLDSPFVKAADRALRRVYGKPAAFARTGGSIPIVAAFRQILGAPVLLVDF
EECCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHCCCEEEEEE
GLNEDAPHSPNESFALEDYHNGILTSAFLLQEIGK
CCCCCCCCCCCCCEEHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA