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The map label for this gene is blt [H]

Identifier: 94985599

GI number: 94985599

Start: 1585441

End: 1586691

Strand: Reverse

Name: blt [H]

Synonym: Dgeo_1499

Alternate gene names: 94985599

Gene position: 1586691-1585441 (Counterclockwise)

Preceding gene: 94985603

Following gene: 94985598

Centisome position: 64.31

GC content: 67.47

Gene sequence:

>1251_bases
ATGACGGCCACGCCTCCTGCCCCGGCTCGGCTCCCGCTGGGCCGGGCCAAGATCATCCTGTTCCTGACCATCTTCATTGC
CATGCTGGGGCTGTCGGTGCTGTTTCCGATTATCGCGCCCCTGGGACGACAGCTTGGACTGACCGAGACGCAGATCGGCT
GGTTTTCCACTGCCTACAGCCTGGCGCAGTTCGTGTTCGCCCCCATCTGGGGCAGCCGCAGTGAACGCACCGGGCGCAAA
CCGGTCCTGCTGCTGGGCCTGATCGGCTTCTCGCTGAGTTTCACGCTGTTTGGACTGTTCGCGTCGTTGGGTGCGCGGGG
CGTTCTGGCGGGAACGGCCCTGTTTGTGCTGTTGGTTGCCTCGCGCCTACTGGGCGGGATGCTCTCCAGCGCCACCCTTC
CCACTGCTCAGGCGATGATGGCTGACCTCAGCAGCGAGAAGGACCGGACCGCCGCGATGGGATTGATCGGCGCGGCCTTC
GGCCTGGGCGTGGTGTTTGGCCCCGCACTGGGCGCGCTGCTTGCGGGTTTCGGGCTGACGGTGCCGATCTTTTTCAGCGC
GGGCCTGGGCCTGCTCACTGCGTTCGCGGCCTACTTCACCCTGCCTGAGACGCGCCGCGCCGATGCCCGGACCGCTGCCC
CCGGCGACCGCCGTGCGCTGCTGCGCCAACCCGGCATCCTGCTGTTCCTGGCGATCAGCACGCTCTACACGCTGGCCAGC
GTGGGCATGGAGCAGACGATTGCCTTCTATGTGCAGGACACGCTGCGCCTCACGGCAGCCCAGACGGCCAAGACAGTCGG
CGGGATGCTCGCCATCTTCGGATTTCTGGCGGCGGCAGTGCAGGGCGGCGCAATGCGGCCCCTGAGCAAGAAGATCGCGC
CCGGCCCGCTGATCATGCTGGGTCTGCTGGTGATGGGCACCGGGATGTTCCTGCTGCCCCTCACCTCAGCGTACTGGACG
ATCACTGCCGCGCTCGCCGTCGTCGGGATTGGCAGCGCCATTCTGGGCCCCAGCCTCAGTGCTGCGCTCTCCCTGAGTGT
GGGGCGCGACGGGCAAGGTGCGGTTGCTGGGCTGAACAGTAGCGCCCTCGCGCTGGGGCGCATGACGGGTCCTCTCATTG
GGACCAGCCTGTATCAGAGTGCCGGGCACGGAGCCCCGTACCTGTTCAGCGGCAGCGTCCTGACGGCTCTGCTGGTCTGG
ACCCTGATCGCCCGGCCCCAGGTGCGGCCCACTGAGGGGGCCAAGGTCTGA

Upstream 100 bases:

>100_bases
CAACTGAGTCCAAAGCGGGAAGTATGCCAGCGTAAACCCACTGTGAATCTCGCCGTGATTTTCCTTACCGACCGGTCAGG
AGTCGTGGTAAACTGGGATC

Downstream 100 bases:

>100_bases
ACCTCTGAGCGGTGGACCAAGAGCTGGAGCCAAGATCTGTGGGCGAGGGCTTTCGGCGAGTGCCGGGGCCTCTGCCTTTT
GGAGGAAAGAGCACGGGGAC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 2 [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTATPPAPARLPLGRAKIILFLTIFIAMLGLSVLFPIIAPLGRQLGLTETQIGWFSTAYSLAQFVFAPIWGSRSERTGRK
PVLLLGLIGFSLSFTLFGLFASLGARGVLAGTALFVLLVASRLLGGMLSSATLPTAQAMMADLSSEKDRTAAMGLIGAAF
GLGVVFGPALGALLAGFGLTVPIFFSAGLGLLTAFAAYFTLPETRRADARTAAPGDRRALLRQPGILLFLAISTLYTLAS
VGMEQTIAFYVQDTLRLTAAQTAKTVGGMLAIFGFLAAAVQGGAMRPLSKKIAPGPLIMLGLLVMGTGMFLLPLTSAYWT
ITAALAVVGIGSAILGPSLSAALSLSVGRDGQGAVAGLNSSALALGRMTGPLIGTSLYQSAGHGAPYLFSGSVLTALLVW
TLIARPQVRPTEGAKV

Sequences:

>Translated_416_residues
MTATPPAPARLPLGRAKIILFLTIFIAMLGLSVLFPIIAPLGRQLGLTETQIGWFSTAYSLAQFVFAPIWGSRSERTGRK
PVLLLGLIGFSLSFTLFGLFASLGARGVLAGTALFVLLVASRLLGGMLSSATLPTAQAMMADLSSEKDRTAAMGLIGAAF
GLGVVFGPALGALLAGFGLTVPIFFSAGLGLLTAFAAYFTLPETRRADARTAAPGDRRALLRQPGILLFLAISTLYTLAS
VGMEQTIAFYVQDTLRLTAAQTAKTVGGMLAIFGFLAAAVQGGAMRPLSKKIAPGPLIMLGLLVMGTGMFLLPLTSAYWT
ITAALAVVGIGSAILGPSLSAALSLSVGRDGQGAVAGLNSSALALGRMTGPLIGTSLYQSAGHGAPYLFSGSVLTALLVW
TLIARPQVRPTEGAKV
>Mature_415_residues
TATPPAPARLPLGRAKIILFLTIFIAMLGLSVLFPIIAPLGRQLGLTETQIGWFSTAYSLAQFVFAPIWGSRSERTGRKP
VLLLGLIGFSLSFTLFGLFASLGARGVLAGTALFVLLVASRLLGGMLSSATLPTAQAMMADLSSEKDRTAAMGLIGAAFG
LGVVFGPALGALLAGFGLTVPIFFSAGLGLLTAFAAYFTLPETRRADARTAAPGDRRALLRQPGILLFLAISTLYTLASV
GMEQTIAFYVQDTLRLTAAQTAKTVGGMLAIFGFLAAAVQGGAMRPLSKKIAPGPLIMLGLLVMGTGMFLLPLTSAYWTI
TAALAVVGIGSAILGPSLSAALSLSVGRDGQGAVAGLNSSALALGRMTGPLIGTSLYQSAGHGAPYLFSGSVLTALLVWT
LIARPQVRPTEGAKV

Specific function: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally div

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI166064062, Length=397, Percent_Identity=27.9596977329975, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI166064060, Length=397, Percent_Identity=27.9596977329975, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI225703104, Length=459, Percent_Identity=26.797385620915, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI225703102, Length=459, Percent_Identity=26.797385620915, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI39753965, Length=330, Percent_Identity=25.7575757575758, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787291, Length=423, Percent_Identity=23.4042553191489, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17551570, Length=367, Percent_Identity=24.5231607629428, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17738085, Length=370, Percent_Identity=27.5675675675676, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42805; Mature: 42673

Theoretical pI: Translated: 11.58; Mature: 11.58

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPPAPARLPLGRAKIILFLTIFIAMLGLSVLFPIIAPLGRQLGLTETQIGWFSTAYS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LAQFVFAPIWGSRSERTGRKPVLLLGLIGFSLSFTLFGLFASLGARGVLAGTALFVLLVA
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SRLLGGMLSSATLPTAQAMMADLSSEKDRTAAMGLIGAAFGLGVVFGPALGALLAGFGLT
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VPIFFSAGLGLLTAFAAYFTLPETRRADARTAAPGDRRALLRQPGILLFLAISTLYTLAS
HHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
VGMEQTIAFYVQDTLRLTAAQTAKTVGGMLAIFGFLAAAVQGGAMRPLSKKIAPGPLIML
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHH
GLLVMGTGMFLLPLTSAYWTITAALAVVGIGSAILGPSLSAALSLSVGRDGQGAVAGLNS
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHEEEECCCCCCCCEECCCC
SALALGRMTGPLIGTSLYQSAGHGAPYLFSGSVLTALLVWTLIARPQVRPTEGAKV
HHHHHHHHHCHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TATPPAPARLPLGRAKIILFLTIFIAMLGLSVLFPIIAPLGRQLGLTETQIGWFSTAYS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LAQFVFAPIWGSRSERTGRKPVLLLGLIGFSLSFTLFGLFASLGARGVLAGTALFVLLVA
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SRLLGGMLSSATLPTAQAMMADLSSEKDRTAAMGLIGAAFGLGVVFGPALGALLAGFGLT
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VPIFFSAGLGLLTAFAAYFTLPETRRADARTAAPGDRRALLRQPGILLFLAISTLYTLAS
HHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
VGMEQTIAFYVQDTLRLTAAQTAKTVGGMLAIFGFLAAAVQGGAMRPLSKKIAPGPLIML
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHH
GLLVMGTGMFLLPLTSAYWTITAALAVVGIGSAILGPSLSAALSLSVGRDGQGAVAGLNS
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHEEEECCCCCCCCEECCCC
SALALGRMTGPLIGTSLYQSAGHGAPYLFSGSVLTALLVWTLIARPQVRPTEGAKV
HHHHHHHHHCHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608059; 8969508; 9384377 [H]