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The map label for this gene is rsmB [H]

Identifier: 94985598

GI number: 94985598

Start: 1583998

End: 1585281

Strand: Reverse

Name: rsmB [H]

Synonym: Dgeo_1498

Alternate gene names: 94985598

Gene position: 1585281-1583998 (Counterclockwise)

Preceding gene: 94985599

Following gene: 94985592

Centisome position: 64.25

GC content: 70.79

Gene sequence:

>1284_bases
ATGACTGCCCCTCGTCCCACCTTTAATCCGGCCCGCGCGCTCGCCGTGCGGGTGCTGACGCGCGTGCTGGCCGGGGAGAC
GTTCGCGGCACCGGCGCTGGACGCGGCGCTTGCGGAGGCCCGGCTTCCCGCACGGGACGCAGGACTGGCGACCCACCTCG
TCTACGGCACGCTCCGCTTTGCACCGATGCTCGAAGCCGCTTTGGCTCCGCTGCTGCGCGAAAAAACCCACTCCAAAACG
CGAGCGTTGCTGCTGGCTGGAGCCTTCGAGAAACTGGTCCTGGGGACGCCGCCGCATGCCGCCCTCAGCGAGTATGTCAA
CGTTGCGCGCATGGCGCGGCTGGCTCCGCCCGGCCTGGTGAATGCGGTGCTGCGCCGGGTGGAGCGGCCCGCCGAGACAG
AAGAAACTCGCTACGCGCTGCCGCGGTGGCTGATCGACATCTTCCGCGCCGCCTACGGACCACGGGCCGACGCCGTGCTC
GCCGATCAGCTCCAGCCGCAGCCGCTCTGGCTGAGTCTGTCCGATGAGGGCGTGCGCCGCCTGGAGGCGGAGGGCAGCGT
GGTGGAACCCGGCCCGAACGGTGTGGACCGCGTGACCCTGGCGCGGCCGCTGCGCGACACCGCGGCCTACCGTCAGGGGC
AGGCGCAGCCCATCAACCCCGCGAGCCTCAGCGTGGTGGATGCGCTCGGGGAGGTAAAAGGCCAGCGCGTGCTTGATCTG
GCGGGGGGCGCGGGTGTAAAGGCCGCGATGCTCGCCGCGCGGGGGGCACACGTCACCAGTGTCGACCTTTTGGCCCGCAA
GCACGACGCGGCCCGGGCCAACCTGAAGCGCTTGGGCCTCCACGCCGACTTCCTCACACACGACCTCACCCGGCCGCTGG
AAGTGCCGCCCGCGCCGCTGGTGCTGCTTGACGCGCCCTGCACCGGCACCGGCACCCTGCGTGCCCACCCCGAAATCAAG
CTGCGGCTGACGCCCGCGGCGGTGGAGGAGATGGCCGCTCTGCAGGCCCGGCTGCTGCCCAATGCGGCTGCGGTGGTCGA
ACCGGGCGGTCTGCTGGTCTACAGTGTGTGCAGCGTGACGCCGCAGGAGGGCCAGGAGGTGGTGCGGGCTTTCCTGGCTG
AGCATCCCGAGTTTATGTCCGAGCCTCTCTCCGACCTGGGTCTGCCCACCGTGTCTGCCGGAGAGGGGGTGCTCACCGTT
CCCGAGGACGGCGTGGACGGGTTTTTCATCGCGCGGCTAAGGCGAGCAGGCGCGCAGGGGACAGCGGCAGGAGCTGAGCG
CTGA

Upstream 100 bases:

>100_bases
GTGCCGGGGCCTCTGCCTTTTGGAGGAAAGAGCACGGGGACTGCCTCCGTCTGTCTGCTCTGGCCTCATCCTCCGCCCCT
CCCGCCGTATCCTGCCCCAC

Downstream 100 bases:

>100_bases
CCGGCCTGTTGGACCGTCCCAGACTGTCCGGATCACATGCTGTTGGAAGGTACTGCCAGTCCCAGTGCGGCGTCCAGCGC
GACCTCAATCATGCCGTTAA

Product: NusB/RsmB/TIM44

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAPRPTFNPARALAVRVLTRVLAGETFAAPALDAALAEARLPARDAGLATHLVYGTLRFAPMLEAALAPLLREKTHSKT
RALLLAGAFEKLVLGTPPHAALSEYVNVARMARLAPPGLVNAVLRRVERPAETEETRYALPRWLIDIFRAAYGPRADAVL
ADQLQPQPLWLSLSDEGVRRLEAEGSVVEPGPNGVDRVTLARPLRDTAAYRQGQAQPINPASLSVVDALGEVKGQRVLDL
AGGAGVKAAMLAARGAHVTSVDLLARKHDAARANLKRLGLHADFLTHDLTRPLEVPPAPLVLLDAPCTGTGTLRAHPEIK
LRLTPAAVEEMAALQARLLPNAAAVVEPGGLLVYSVCSVTPQEGQEVVRAFLAEHPEFMSEPLSDLGLPTVSAGEGVLTV
PEDGVDGFFIARLRRAGAQGTAAGAER

Sequences:

>Translated_427_residues
MTAPRPTFNPARALAVRVLTRVLAGETFAAPALDAALAEARLPARDAGLATHLVYGTLRFAPMLEAALAPLLREKTHSKT
RALLLAGAFEKLVLGTPPHAALSEYVNVARMARLAPPGLVNAVLRRVERPAETEETRYALPRWLIDIFRAAYGPRADAVL
ADQLQPQPLWLSLSDEGVRRLEAEGSVVEPGPNGVDRVTLARPLRDTAAYRQGQAQPINPASLSVVDALGEVKGQRVLDL
AGGAGVKAAMLAARGAHVTSVDLLARKHDAARANLKRLGLHADFLTHDLTRPLEVPPAPLVLLDAPCTGTGTLRAHPEIK
LRLTPAAVEEMAALQARLLPNAAAVVEPGGLLVYSVCSVTPQEGQEVVRAFLAEHPEFMSEPLSDLGLPTVSAGEGVLTV
PEDGVDGFFIARLRRAGAQGTAAGAER
>Mature_426_residues
TAPRPTFNPARALAVRVLTRVLAGETFAAPALDAALAEARLPARDAGLATHLVYGTLRFAPMLEAALAPLLREKTHSKTR
ALLLAGAFEKLVLGTPPHAALSEYVNVARMARLAPPGLVNAVLRRVERPAETEETRYALPRWLIDIFRAAYGPRADAVLA
DQLQPQPLWLSLSDEGVRRLEAEGSVVEPGPNGVDRVTLARPLRDTAAYRQGQAQPINPASLSVVDALGEVKGQRVLDLA
GGAGVKAAMLAARGAHVTSVDLLARKHDAARANLKRLGLHADFLTHDLTRPLEVPPAPLVLLDAPCTGTGTLRAHPEIKL
RLTPAAVEEMAALQARLLPNAAAVVEPGGLLVYSVCSVTPQEGQEVVRAFLAEHPEFMSEPLSDLGLPTVSAGEGVLTVP
EDGVDGFFIARLRRAGAQGTAAGAER

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=239, Percent_Identity=28.4518828451883, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI76150623, Length=239, Percent_Identity=28.4518828451883, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI2367212, Length=399, Percent_Identity=29.0726817042606, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI87081985, Length=199, Percent_Identity=35.678391959799, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324268, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI22024126, Length=240, Percent_Identity=27.9166666666667, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 45218; Mature: 45086

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPRPTFNPARALAVRVLTRVLAGETFAAPALDAALAEARLPARDAGLATHLVYGTLRF
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHH
APMLEAALAPLLREKTHSKTRALLLAGAFEKLVLGTPPHAALSEYVNVARMARLAPPGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHH
NAVLRRVERPAETEETRYALPRWLIDIFRAAYGPRADAVLADQLQPQPLWLSLSDEGVRR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECHHHHHH
LEAEGSVVEPGPNGVDRVTLARPLRDTAAYRQGQAQPINPASLSVVDALGEVKGQRVLDL
HHCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
AGGAGVKAAMLAARGAHVTSVDLLARKHDAARANLKRLGLHADFLTHDLTRPLEVPPAPL
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCE
VLLDAPCTGTGTLRAHPEIKLRLTPAAVEEMAALQARLLPNAAAVVEPGGLLVYSVCSVT
EEEECCCCCCCCEEECCCEEEEECHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHCCC
PQEGQEVVRAFLAEHPEFMSEPLSDLGLPTVSAGEGVLTVPEDGVDGFFIARLRRAGAQG
HHHHHHHHHHHHHHCHHHHHCCHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCC
TAAGAER
CCCCCCC
>Mature Secondary Structure 
TAPRPTFNPARALAVRVLTRVLAGETFAAPALDAALAEARLPARDAGLATHLVYGTLRF
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHH
APMLEAALAPLLREKTHSKTRALLLAGAFEKLVLGTPPHAALSEYVNVARMARLAPPGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHH
NAVLRRVERPAETEETRYALPRWLIDIFRAAYGPRADAVLADQLQPQPLWLSLSDEGVRR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEECHHHHHH
LEAEGSVVEPGPNGVDRVTLARPLRDTAAYRQGQAQPINPASLSVVDALGEVKGQRVLDL
HHCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
AGGAGVKAAMLAARGAHVTSVDLLARKHDAARANLKRLGLHADFLTHDLTRPLEVPPAPL
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCE
VLLDAPCTGTGTLRAHPEIKLRLTPAAVEEMAALQARLLPNAAAVVEPGGLLVYSVCSVT
EEEECCCCCCCCEEECCCEEEEECHHHHHHHHHHHHHHCCCCCEEECCCCEEEEHHHCCC
PQEGQEVVRAFLAEHPEFMSEPLSDLGLPTVSAGEGVLTVPEDGVDGFFIARLRRAGAQG
HHHHHHHHHHHHHHCHHHHHCCHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHCCCCC
TAAGAER
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA