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The map label for this gene is prfC [H]

Identifier: 94985592

GI number: 94985592

Start: 1578514

End: 1580217

Strand: Reverse

Name: prfC [H]

Synonym: Dgeo_1492

Alternate gene names: 94985592

Gene position: 1580217-1578514 (Counterclockwise)

Preceding gene: 94985598

Following gene: 94985591

Centisome position: 64.05

GC content: 66.02

Gene sequence:

>1704_bases
ATGTCCGAATGGTGCATCTCCTCCCGCCCCCGTAGCGCGAGTCGCAGGTGCCGCGTTTTCCTCCGTTGCCTTTCCTTCAA
GCCGGGCATCCTTGTCTGCCCGCAGGAGTGTTCTGTGACCATCCCCACCGCTCAACTGGAGCACGAAATCACCCGCCGCC
GCACCTTCGCCATCATTTCCCACCCGGATGCCGGGAAGACAACCATCACCGAGAAGCTGCTGCTGTACGGAGGCGCGATT
CAGGAGGCAGGCAGCGTGACGGCCAAGGAAGGCCGCGCCCACACGAAGTCCGACTGGATGAGCATCGAGCAGCAGCGCGG
GATTTCCATCTCCTCCTCCGCGCTGACCTTTGAGTACGCCGGGCGCCACATCAACCTGCTCGACACGCCCGGCCACCAGG
ATTTTTCCGAGGACACCTACCGGACCCTCACCGCCGCCGACTCCGCCCTGATGGTGCTCGACGCGGCGCGCGGCGTGCAG
TCGCAGACGGAAAAGCTCTTTGCGGTGTGCCGCAACCGCGGGATTCCCATCCTCACCTTCGTGAACAAGATGGACCGCCC
GGCGCTGGACCCCTTCGAGCTGCTCGCGCAGGTGGAGAACACCCTCAAGATCACGGCCGTGCCCCTCACCTGGCCGATTG
GCGACGGCCCCGATTTCAAGGGCGTCTACGATCTCCAGACTGGGCAGGTTCTCGTCTTTGAGCGCACGTCGGGCGGCAAA
CACCGCGCCCCGGTGCAGACCGCGGGGCTGACGGACCCCCAACTTGCCGAACTGGTGGGGCCGGACCTCGCGGCCAAGTT
GCAGGAGGATGTGGACCTGATTCGGGGAGCGATGCCAGAGTTTGACCCCGCCGCCTTCCGGTCGGGCGAACTCACCCCGG
TCTTCTTCGGGTCCGCCATGAACAACTTCGGCGTCGAGCACTTCCTCGCGAACTTCGTGGAGCTTGCGCCGCCCCCCGGC
CCCGTCGAGACGAACCTGGGCAAACGCGCCCCAGACGCGCCGTTTGCGGGGTTCATCTTCAAGCTCCAGGCCAACATGAG
CCGGGCCCACCGCGACCGCACCGCCTACATGCGGGTGATGTCCGGGCACTTTACGCGCGGCATGGACGTGACCCACACCC
GCACCGGGCGGAAGCTGCGGCTCTCGCAGGCCCATACCCTCTTTGCCCAGGACCGCGAAAAGGTGGAGGAGGCGTATCCC
GGCGACATCGTGGGCCTGGTGAACCCCGGTGTCTTTCAGATCGGGGACGTGGTGAGCGTGGACCCCAAGGTGACGCTGCC
TTCCTTTCCGCGCTTCACGCCGGAAACCTTCGCCACCCTCTCCCTGAGGGATGTGGGCAAGCGCAAGGCGTTCATGAAGG
GCCTCACCCAGCTTGCCGAAGAAGGCGTCGTGCAGGTCTTTTATCCCACCGACGGCGCCCGCGACCCCTATCTCGGGGCG
GTCGGCCCCCTCCAGTTCGAGGTCTTCCAGGCCCGCTTGCAGGAGGAGTACGGCGTGGAGGTGGATCTCCACGTGACCGG
CTACCAGCTCGTGCGCTGGCTGGCCGGTGATCCGGGCAGCGTGGCCCGCTTTGCCCGGCACGTGGAGGATGACCAGGGCC
GCCCGGTGATGCTCTTCCGCTCCCGCTATGACCTGGAGTACACCGCCGAGCAGCACCCGGAAATCGAGTTTCTGCCGCTG
CCCAAAGACCTCACGCGGGTGTGA

Upstream 100 bases:

>100_bases
CGCCCGCCGCGTGAACGTGACGGGGGTGTAGGGCGGCCAGGGAGGGGAGCACGCTGCCCTCGTCCTCCCGGCGGTGCTCG
GCCTCCTCTCCTGCAGTATC

Downstream 100 bases:

>100_bases
GATGAACCTGGAACGTGTCCCGCCCGCCTTTTTTTCCATGCCCGCCGTCAGTGCGGCGCTCAACCTGAATGCGAAGGCGG
TTCGGGACTTGGGTCTTACT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSEWCISSRPRSASRRCRVFLRCLSFKPGILVCPQECSVTIPTAQLEHEITRRRTFAIISHPDAGKTTITEKLLLYGGAI
QEAGSVTAKEGRAHTKSDWMSIEQQRGISISSSALTFEYAGRHINLLDTPGHQDFSEDTYRTLTAADSALMVLDAARGVQ
SQTEKLFAVCRNRGIPILTFVNKMDRPALDPFELLAQVENTLKITAVPLTWPIGDGPDFKGVYDLQTGQVLVFERTSGGK
HRAPVQTAGLTDPQLAELVGPDLAAKLQEDVDLIRGAMPEFDPAAFRSGELTPVFFGSAMNNFGVEHFLANFVELAPPPG
PVETNLGKRAPDAPFAGFIFKLQANMSRAHRDRTAYMRVMSGHFTRGMDVTHTRTGRKLRLSQAHTLFAQDREKVEEAYP
GDIVGLVNPGVFQIGDVVSVDPKVTLPSFPRFTPETFATLSLRDVGKRKAFMKGLTQLAEEGVVQVFYPTDGARDPYLGA
VGPLQFEVFQARLQEEYGVEVDLHVTGYQLVRWLAGDPGSVARFARHVEDDQGRPVMLFRSRYDLEYTAEQHPEIEFLPL
PKDLTRV

Sequences:

>Translated_567_residues
MSEWCISSRPRSASRRCRVFLRCLSFKPGILVCPQECSVTIPTAQLEHEITRRRTFAIISHPDAGKTTITEKLLLYGGAI
QEAGSVTAKEGRAHTKSDWMSIEQQRGISISSSALTFEYAGRHINLLDTPGHQDFSEDTYRTLTAADSALMVLDAARGVQ
SQTEKLFAVCRNRGIPILTFVNKMDRPALDPFELLAQVENTLKITAVPLTWPIGDGPDFKGVYDLQTGQVLVFERTSGGK
HRAPVQTAGLTDPQLAELVGPDLAAKLQEDVDLIRGAMPEFDPAAFRSGELTPVFFGSAMNNFGVEHFLANFVELAPPPG
PVETNLGKRAPDAPFAGFIFKLQANMSRAHRDRTAYMRVMSGHFTRGMDVTHTRTGRKLRLSQAHTLFAQDREKVEEAYP
GDIVGLVNPGVFQIGDVVSVDPKVTLPSFPRFTPETFATLSLRDVGKRKAFMKGLTQLAEEGVVQVFYPTDGARDPYLGA
VGPLQFEVFQARLQEEYGVEVDLHVTGYQLVRWLAGDPGSVARFARHVEDDQGRPVMLFRSRYDLEYTAEQHPEIEFLPL
PKDLTRV
>Mature_566_residues
SEWCISSRPRSASRRCRVFLRCLSFKPGILVCPQECSVTIPTAQLEHEITRRRTFAIISHPDAGKTTITEKLLLYGGAIQ
EAGSVTAKEGRAHTKSDWMSIEQQRGISISSSALTFEYAGRHINLLDTPGHQDFSEDTYRTLTAADSALMVLDAARGVQS
QTEKLFAVCRNRGIPILTFVNKMDRPALDPFELLAQVENTLKITAVPLTWPIGDGPDFKGVYDLQTGQVLVFERTSGGKH
RAPVQTAGLTDPQLAELVGPDLAAKLQEDVDLIRGAMPEFDPAAFRSGELTPVFFGSAMNNFGVEHFLANFVELAPPPGP
VETNLGKRAPDAPFAGFIFKLQANMSRAHRDRTAYMRVMSGHFTRGMDVTHTRTGRKLRLSQAHTLFAQDREKVEEAYPG
DIVGLVNPGVFQIGDVVSVDPKVTLPSFPRFTPETFATLSLRDVGKRKAFMKGLTQLAEEGVVQVFYPTDGARDPYLGAV
GPLQFEVFQARLQEEYGVEVDLHVTGYQLVRWLAGDPGSVARFARHVEDDQGRPVMLFRSRYDLEYTAEQHPEIEFLPLP
KDLTRV

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=504, Percent_Identity=29.1666666666667, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI19923640, Length=502, Percent_Identity=26.0956175298805, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI25306283, Length=391, Percent_Identity=28.3887468030691, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI25306287, Length=292, Percent_Identity=32.1917808219178, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI157426893, Length=155, Percent_Identity=32.258064516129, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI94966754, Length=277, Percent_Identity=27.7978339350181, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4503483, Length=150, Percent_Identity=36, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI310132016, Length=124, Percent_Identity=34.6774193548387, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI310110807, Length=124, Percent_Identity=34.6774193548387, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI310123363, Length=124, Percent_Identity=34.6774193548387, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790835, Length=517, Percent_Identity=48.5493230174081, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1789738, Length=495, Percent_Identity=27.8787878787879, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788922, Length=158, Percent_Identity=34.1772151898734, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI48994988, Length=179, Percent_Identity=31.8435754189944, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789737, Length=133, Percent_Identity=32.3308270676692, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790412, Length=133, Percent_Identity=32.3308270676692, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17533571, Length=501, Percent_Identity=29.7405189620758, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17556745, Length=182, Percent_Identity=36.2637362637363, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17557151, Length=147, Percent_Identity=32.6530612244898, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=201, Percent_Identity=31.8407960199005, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=32.5925925925926, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=32.5925925925926, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=498, Percent_Identity=28.714859437751, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6322359, Length=513, Percent_Identity=26.1208576998051, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323320, Length=176, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=161, Percent_Identity=32.2981366459627, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320593, Length=161, Percent_Identity=32.2981366459627, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=156, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24582462, Length=489, Percent_Identity=30.2658486707566, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI221458488, Length=510, Percent_Identity=26.6666666666667, Blast_Score=152, Evalue=5e-37,
Organism=Drosophila melanogaster, GI78706572, Length=148, Percent_Identity=34.4594594594595, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585709, Length=157, Percent_Identity=33.7579617834395, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24585711, Length=157, Percent_Identity=33.7579617834395, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24585713, Length=157, Percent_Identity=33.7579617834395, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 62878; Mature: 62747

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEWCISSRPRSASRRCRVFLRCLSFKPGILVCPQECSVTIPTAQLEHEITRRRTFAIIS
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCEECCHHHHHHHHHHCEEEEEEE
HPDAGKTTITEKLLLYGGAIQEAGSVTAKEGRAHTKSDWMSIEQQRGISISSSALTFEYA
CCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCEEEEEEC
GRHINLLDTPGHQDFSEDTYRTLTAADSALMVLDAARGVQSQTEKLFAVCRNRGIPILTF
CCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEHHHCCHHHHHHHHHHHHHCCCCEEEEH
VNKMDRPALDPFELLAQVENTLKITAVPLTWPIGDGPDFKGVYDLQTGQVLVFERTSGGK
HHHCCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCC
HRAPVQTAGLTDPQLAELVGPDLAAKLQEDVDLIRGAMPEFDPAAFRSGELTPVFFGSAM
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEEECCHH
NNFGVEHFLANFVELAPPPGPVETNLGKRAPDAPFAGFIFKLQANMSRAHRDRTAYMRVM
HHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
SGHFTRGMDVTHTRTGRKLRLSQAHTLFAQDREKVEEAYPGDIVGLVNPGVFQIGDVVSV
HCCCCCCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCEEEC
DPKVTLPSFPRFTPETFATLSLRDVGKRKAFMKGLTQLAEEGVVQVFYPTDGARDPYLGA
CCCCCCCCCCCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCC
VGPLQFEVFQARLQEEYGVEVDLHVTGYQLVRWLAGDPGSVARFARHVEDDQGRPVMLFR
CCCHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEE
SRYDLEYTAEQHPEIEFLPLPKDLTRV
CCCCEEECCCCCCCEEEEECCHHHHCC
>Mature Secondary Structure 
SEWCISSRPRSASRRCRVFLRCLSFKPGILVCPQECSVTIPTAQLEHEITRRRTFAIIS
CCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCEECCHHHHHHHHHHCEEEEEEE
HPDAGKTTITEKLLLYGGAIQEAGSVTAKEGRAHTKSDWMSIEQQRGISISSSALTFEYA
CCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEECCCEEEEEEC
GRHINLLDTPGHQDFSEDTYRTLTAADSALMVLDAARGVQSQTEKLFAVCRNRGIPILTF
CCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEHHHCCHHHHHHHHHHHHHCCCCEEEEH
VNKMDRPALDPFELLAQVENTLKITAVPLTWPIGDGPDFKGVYDLQTGQVLVFERTSGGK
HHHCCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCC
HRAPVQTAGLTDPQLAELVGPDLAAKLQEDVDLIRGAMPEFDPAAFRSGELTPVFFGSAM
CCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEEECCHH
NNFGVEHFLANFVELAPPPGPVETNLGKRAPDAPFAGFIFKLQANMSRAHRDRTAYMRVM
HHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
SGHFTRGMDVTHTRTGRKLRLSQAHTLFAQDREKVEEAYPGDIVGLVNPGVFQIGDVVSV
HCCCCCCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCEEEC
DPKVTLPSFPRFTPETFATLSLRDVGKRKAFMKGLTQLAEEGVVQVFYPTDGARDPYLGA
CCCCCCCCCCCCCCCHHEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCC
VGPLQFEVFQARLQEEYGVEVDLHVTGYQLVRWLAGDPGSVARFARHVEDDQGRPVMLFR
CCCHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEE
SRYDLEYTAEQHPEIEFLPLPKDLTRV
CCCCEEECCCCCCCEEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA