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The map label for this gene is buk [H]

Identifier: 94985483

GI number: 94985483

Start: 1464348

End: 1465418

Strand: Reverse

Name: buk [H]

Synonym: Dgeo_1383

Alternate gene names: 94985483

Gene position: 1465418-1464348 (Counterclockwise)

Preceding gene: 94985484

Following gene: 94985482

Centisome position: 59.4

GC content: 69.47

Gene sequence:

>1071_bases
ATGATCGCGCATGTGATCAATCCCGGTTCCAGCAGCGTGAAACTCGCTTGCGCCAGCATCCTCCCCAGCGAGAACGCGGC
TCTGCCTGGCCAGTTGCGCGTGGCGCTGACGCGCACTGAGGTGCCCCTTCCCGGTCCACCGGGAGAACAGGATCTGGCGA
CCCTGGCCTCGGCCGTGCTGGACGCCACTGCAGACTGGCCCTTTCCCGACGCCGTGGTGGCGCGGGGTGGCTGGCTGGGC
CGGGTCGCTGCGGGCACCTACCGGGTTACGCCGGAGCTGGCGCATTACGCCGCTCAGGAAGGGCGGGATGGCCTGGGTGC
GGTGCTGGCCCTCCGGGTGGGGGAGGCGCGTGGCGTGCCCGCTTTTGTTGTGGACCCCCAGAGTGTCAACGAACTACTGC
CGGAAGCCCGCGAGACGGGAGTACGGGGAGTCATACGCGAGGCGCGTTTTCATGCGCTGAACGCCCGGATGGTTGCCCGC
CGCGCTGCCCACGAGGTGGGTAAGCGCTTGCAGGATGCCCGAGTGGTGGTCGCGCATCTGGGGGCAACCACCAGCGTGAC
AGCCTTTGATGGTGGCCGGGCGATCGACACCACCGGGACTGGCCCCGAGGGCGGTCCACTGGGTGCCTTGCAGGCCGGAC
CACTGCCCACTTCCGCGCTGCTGCGCCTGACGGAAGGCCGCTCGCCGGCCGAACTGCTGCGGCTGTTGGGAGCGGAGAGC
GGCTTTCTGGCCCTGACCGGCAGCGCCAATCTCAAGGAGCTTGAGGCGCGCGAGGCCACCGATCCGGCTGTCCAGGCCGC
CGCCGCCGCCTTTGTGCATCAGGCGTGCAAGGCGATCGGCGAGCAGTGCGGAGCCTTGTCCGGTCGCCCCGACGCGCTCG
CCCTCACTGGAGGGGCAGCGCGTTGGGAGGCGCTTGTTGACCGTATCGAGCGGCGCCTGAGCTGGATTGCGCCGGTCATT
ATTGTGCCGGGCGAACTCGAACTCGAGGCCTTGGCTGAAGGCGCGGGCCGGGTGTTGTTGGGTCTAGAACAGCCCCGCGA
CTGGACGCCGCCGCTGGGTGGGACGCCCTGA

Upstream 100 bases:

>100_bases
GCTGATCGGGAATGAGGGGAGGAGCTGAGCGAAGCTCTTCCTCTCATTTTCCTCATTGCCGGGACGCTTGCCCTTTGGCA
TCTGCTACCCTGGGGCGTTC

Downstream 100 bases:

>100_bases
CGTGGCCCGTCGCCGCCTGCCCCGCGCCGCCCCCGAGCCGATTCGTGCCACCAGCATGTGGCGGGTGGATCAGGTTTTTC
TCGCGCGCCGTGGCCAGCGC

Product: butyrate kinase

Products: NA

Alternate protein names: BK; Branched-chain carboxylic acid kinase [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MIAHVINPGSSSVKLACASILPSENAALPGQLRVALTRTEVPLPGPPGEQDLATLASAVLDATADWPFPDAVVARGGWLG
RVAAGTYRVTPELAHYAAQEGRDGLGAVLALRVGEARGVPAFVVDPQSVNELLPEARETGVRGVIREARFHALNARMVAR
RAAHEVGKRLQDARVVVAHLGATTSVTAFDGGRAIDTTGTGPEGGPLGALQAGPLPTSALLRLTEGRSPAELLRLLGAES
GFLALTGSANLKELEAREATDPAVQAAAAAFVHQACKAIGEQCGALSGRPDALALTGGAARWEALVDRIERRLSWIAPVI
IVPGELELEALAEGAGRVLLGLEQPRDWTPPLGGTP

Sequences:

>Translated_356_residues
MIAHVINPGSSSVKLACASILPSENAALPGQLRVALTRTEVPLPGPPGEQDLATLASAVLDATADWPFPDAVVARGGWLG
RVAAGTYRVTPELAHYAAQEGRDGLGAVLALRVGEARGVPAFVVDPQSVNELLPEARETGVRGVIREARFHALNARMVAR
RAAHEVGKRLQDARVVVAHLGATTSVTAFDGGRAIDTTGTGPEGGPLGALQAGPLPTSALLRLTEGRSPAELLRLLGAES
GFLALTGSANLKELEAREATDPAVQAAAAAFVHQACKAIGEQCGALSGRPDALALTGGAARWEALVDRIERRLSWIAPVI
IVPGELELEALAEGAGRVLLGLEQPRDWTPPLGGTP
>Mature_356_residues
MIAHVINPGSSSVKLACASILPSENAALPGQLRVALTRTEVPLPGPPGEQDLATLASAVLDATADWPFPDAVVARGGWLG
RVAAGTYRVTPELAHYAAQEGRDGLGAVLALRVGEARGVPAFVVDPQSVNELLPEARETGVRGVIREARFHALNARMVAR
RAAHEVGKRLQDARVVVAHLGATTSVTAFDGGRAIDTTGTGPEGGPLGALQAGPLPTSALLRLTEGRSPAELLRLLGAES
GFLALTGSANLKELEAREATDPAVQAAAAAFVHQACKAIGEQCGALSGRPDALALTGGAARWEALVDRIERRLSWIAPVI
IVPGELELEALAEGAGRVLLGLEQPRDWTPPLGGTP

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=166, Percent_Identity=29.5180722891566, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI145693184, Length=171, Percent_Identity=30.4093567251462, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 36783; Mature: 36783

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAHVINPGSSSVKLACASILPSENAALPGQLRVALTRTEVPLPGPPGEQDLATLASAVL
CEEEEECCCCCCCEEHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
DATADWPFPDAVVARGGWLGRVAAGTYRVTPELAHYAAQEGRDGLGAVLALRVGEARGVP
HCCCCCCCCHHHHHCCCCEEECCCCEEEECHHHHHHHHHCCCCCHHHHHHEEECCCCCCC
AFVVDPQSVNELLPEARETGVRGVIREARFHALNARMVARRAAHEVGKRLQDARVVVAHL
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
GATTSVTAFDGGRAIDTTGTGPEGGPLGALQAGPLPTSALLRLTEGRSPAELLRLLGAES
CCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCC
GFLALTGSANLKELEAREATDPAVQAAAAAFVHQACKAIGEQCGALSGRPDALALTGGAA
CEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHH
RWEALVDRIERRLSWIAPVIIVPGELELEALAEGAGRVLLGLEQPRDWTPPLGGTP
HHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MIAHVINPGSSSVKLACASILPSENAALPGQLRVALTRTEVPLPGPPGEQDLATLASAVL
CEEEEECCCCCCCEEHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
DATADWPFPDAVVARGGWLGRVAAGTYRVTPELAHYAAQEGRDGLGAVLALRVGEARGVP
HCCCCCCCCHHHHHCCCCEEECCCCEEEECHHHHHHHHHCCCCCHHHHHHEEECCCCCCC
AFVVDPQSVNELLPEARETGVRGVIREARFHALNARMVARRAAHEVGKRLQDARVVVAHL
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
GATTSVTAFDGGRAIDTTGTGPEGGPLGALQAGPLPTSALLRLTEGRSPAELLRLLGAES
CCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCC
GFLALTGSANLKELEAREATDPAVQAAAAAFVHQACKAIGEQCGALSGRPDALALTGGAA
CEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHH
RWEALVDRIERRLSWIAPVIIVPGELELEALAEGAGRVLLGLEQPRDWTPPLGGTP
HHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]