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The map label for this gene is cobQ [H]

Identifier: 94972337

GI number: 94972337

Start: 147369

End: 148766

Strand: Direct

Name: cobQ [H]

Synonym: Dgeo_2874

Alternate gene names: 94972337

Gene position: 147369-148766 (Clockwise)

Preceding gene: 94972338

Following gene: 94972336

Centisome position: 25.67

GC content: 70.03

Gene sequence:

>1398_bases
ATGATTCAGGGCTGCACCAGCAGTGCAGGCAAGAGTTATCTGTGCGCCGCCCTGTGCCGCGCCCTTGCGAACGAGGGCTA
CCGGGTCGCGCCCTTCAAAGCCCAGAACATGAGCAACAACGCCGGCGTCACGCCCGCTGGGTTGGAGATGGGCCGTGCCC
AACTCGTGCAGGCGCAGGCTGCCCGCGTGGTGCCCGACGTGCGCATGAATCCTGTGCTGCTCAAGCCCGAGGCTGACACC
CGCTCGCAGGTCGTGCTGCTGGGCCGCGCGAACCCTGGTCTCAGCGCCCTGGGCTGGCGCGAGCGCAAAGCGCACCTCTG
GCCCCACGTGCAGGCGGCCCTGCACAGTCTCCTGGCTGAGTTCGACCTGATCGTTATCGAGGGCGCAGGCAGCCCCGCCG
AGGTGAACCTGCGCGCGTCCGACATCGTGAACATGCGGGTGGCGCTGGAGGCGCGGGCGTCCGTGCTGCTCGCCTGCGAT
ATCGACCGCGGCGGAGCCTTTGCGCACCTGCTGGGCACCTGGCACTGCCTGACGCCGGAGGAGCGCGCCCTCCTCAAAGG
CTTCATCCTCAACCGCTTCCGGGGCGACCCGCGCCTGCTGGCTCCCGCCCCCGCCTGGCTGGAAGAACAGACCGGTGTGC
CGACGGTGGGGGTGGTGCCCCTGCTGGACATTCCCCTCCCGGAGGAGGACGGCGTCTGGGCAGAGGACCAAGGTCCAAGC
GCTTCGGCTTCGAGCGTGGGCGGCTTTGTCGCCATTCCCCGCCTGCCGCGCGTGTCCAATCTGGACGAGTTCGCACCGCT
GGGGCCGCTCGCGCGCTGGGTCTCCACCCCCGCCGAACTGGAGGGTGCGCGGGCGGTCATCCTGCCCGGCAGCAAGAGCA
CCGCCGCCGATCTCGCGTGGCTGCGGGCGAGCGGGCTGGCGGGGGCGATCACGCGGGTGGCGCAGGCCGGGGTGCCGGTC
CTGGGGATCTGCGGAGGCTTGCAGATGCTGGGCCGCCGCATCCGCGACCCCCATGGTGTGGAGGGTGGGGGAGAGGTGCC
CGGCCTAGGCCTGCTCGATCTGGAGACCGAGTTCGCGCCGGGCAAGACCACCCGCCTCACGGCCTGCACCGATGCCGAAA
CCGGCCTGCGGCTGCACGGGTACGAGATTCATCACGGGCAGACGGTGAGCGGTTTGGGAGTGCAAGAACTCGTGCCCGAG
CTGCTGTGGCGCTCCGGCAACGTGCGCGGCACCTATCTGCACGGCCTGCTGGAAAACCCCGCCTACCTCGAACGCTTCCT
GACCTGGGCAGGCCTCACGCCGCCCGTTCCGCTGGCCAGCCTGGACGCGCGACTCGACGCCATTGCTGAGCGCGTCCGGG
TCAGCTTGGACTGGGACTTTGTCCGGAGGCTCTTGTGA

Upstream 100 bases:

>100_bases
ACCCGCCTTCCCGCCGAGAATGCGCGGTTGGTGGCGGCGTTGCGGCAGATCCTGAAGGGAGAACACACGTTGAAGGAAGA
ACACTGTGGGTAAGGCCATC

Downstream 100 bases:

>100_bases
TTGTGTTTGTGACCGGCGGCGCTCGCAGCGGCAAGAGCACGTTTGCCGAGCGCCGCGCTGCCGCCTCCGGCGAGGCCGTC
ACCTACCTTGCCACCGCCCA

Product: cobyric acid synthase CobQ

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MIQGCTSSAGKSYLCAALCRALANEGYRVAPFKAQNMSNNAGVTPAGLEMGRAQLVQAQAARVVPDVRMNPVLLKPEADT
RSQVVLLGRANPGLSALGWRERKAHLWPHVQAALHSLLAEFDLIVIEGAGSPAEVNLRASDIVNMRVALEARASVLLACD
IDRGGAFAHLLGTWHCLTPEERALLKGFILNRFRGDPRLLAPAPAWLEEQTGVPTVGVVPLLDIPLPEEDGVWAEDQGPS
ASASSVGGFVAIPRLPRVSNLDEFAPLGPLARWVSTPAELEGARAVILPGSKSTAADLAWLRASGLAGAITRVAQAGVPV
LGICGGLQMLGRRIRDPHGVEGGGEVPGLGLLDLETEFAPGKTTRLTACTDAETGLRLHGYEIHHGQTVSGLGVQELVPE
LLWRSGNVRGTYLHGLLENPAYLERFLTWAGLTPPVPLASLDARLDAIAERVRVSLDWDFVRRLL

Sequences:

>Translated_465_residues
MIQGCTSSAGKSYLCAALCRALANEGYRVAPFKAQNMSNNAGVTPAGLEMGRAQLVQAQAARVVPDVRMNPVLLKPEADT
RSQVVLLGRANPGLSALGWRERKAHLWPHVQAALHSLLAEFDLIVIEGAGSPAEVNLRASDIVNMRVALEARASVLLACD
IDRGGAFAHLLGTWHCLTPEERALLKGFILNRFRGDPRLLAPAPAWLEEQTGVPTVGVVPLLDIPLPEEDGVWAEDQGPS
ASASSVGGFVAIPRLPRVSNLDEFAPLGPLARWVSTPAELEGARAVILPGSKSTAADLAWLRASGLAGAITRVAQAGVPV
LGICGGLQMLGRRIRDPHGVEGGGEVPGLGLLDLETEFAPGKTTRLTACTDAETGLRLHGYEIHHGQTVSGLGVQELVPE
LLWRSGNVRGTYLHGLLENPAYLERFLTWAGLTPPVPLASLDARLDAIAERVRVSLDWDFVRRLL
>Mature_465_residues
MIQGCTSSAGKSYLCAALCRALANEGYRVAPFKAQNMSNNAGVTPAGLEMGRAQLVQAQAARVVPDVRMNPVLLKPEADT
RSQVVLLGRANPGLSALGWRERKAHLWPHVQAALHSLLAEFDLIVIEGAGSPAEVNLRASDIVNMRVALEARASVLLACD
IDRGGAFAHLLGTWHCLTPEERALLKGFILNRFRGDPRLLAPAPAWLEEQTGVPTVGVVPLLDIPLPEEDGVWAEDQGPS
ASASSVGGFVAIPRLPRVSNLDEFAPLGPLARWVSTPAELEGARAVILPGSKSTAADLAWLRASGLAGAITRVAQAGVPV
LGICGGLQMLGRRIRDPHGVEGGGEVPGLGLLDLETEFAPGKTTRLTACTDAETGLRLHGYEIHHGQTVSGLGVQELVPE
LLWRSGNVRGTYLHGLLENPAYLERFLTWAGLTPPVPLASLDARLDAIAERVRVSLDWDFVRRLL

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 49533; Mature: 49533

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQGCTSSAGKSYLCAALCRALANEGYRVAPFKAQNMSNNAGVTPAGLEMGRAQLVQAQA
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHH
ARVVPDVRMNPVLLKPEADTRSQVVLLGRANPGLSALGWRERKAHLWPHVQAALHSLLAE
HHCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
FDLIVIEGAGSPAEVNLRASDIVNMRVALEARASVLLACDIDRGGAFAHLLGTWHCLTPE
CCEEEEECCCCCCEEEEEHHHHEEEEEEEECCCEEEEEEECCCCCCHHHHHHCEEECCCH
ERALLKGFILNRFRGDPRLLAPAPAWLEEQTGVPTVGVVPLLDIPLPEEDGVWAEDQGPS
HHHHHHHHHHHHHCCCCCEECCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
ASASSVGGFVAIPRLPRVSNLDEFAPLGPLARWVSTPAELEGARAVILPGSKSTAADLAW
CCCCCCCCEEEECCCCCCCCCHHHCCCHHHHHHHCCCHHCCCCEEEEECCCCCCHHHHHH
LRASGLAGAITRVAQAGVPVLGICGGLQMLGRRIRDPHGVEGGGEVPGLGLLDLETEFAP
HHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCC
GKTTRLTACTDAETGLRLHGYEIHHGQTVSGLGVQELVPELLWRSGNVRGTYLHGLLENP
CCCEEEEEECCCCCCCEEEEEEEECCCEECCCCHHHHHHHHHHCCCCCCEEEEHHHHCCH
AYLERFLTWAGLTPPVPLASLDARLDAIAERVRVSLDWDFVRRLL
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEEECHHHHHHHC
>Mature Secondary Structure
MIQGCTSSAGKSYLCAALCRALANEGYRVAPFKAQNMSNNAGVTPAGLEMGRAQLVQAQA
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHH
ARVVPDVRMNPVLLKPEADTRSQVVLLGRANPGLSALGWRERKAHLWPHVQAALHSLLAE
HHCCCCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
FDLIVIEGAGSPAEVNLRASDIVNMRVALEARASVLLACDIDRGGAFAHLLGTWHCLTPE
CCEEEEECCCCCCEEEEEHHHHEEEEEEEECCCEEEEEEECCCCCCHHHHHHCEEECCCH
ERALLKGFILNRFRGDPRLLAPAPAWLEEQTGVPTVGVVPLLDIPLPEEDGVWAEDQGPS
HHHHHHHHHHHHHCCCCCEECCCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
ASASSVGGFVAIPRLPRVSNLDEFAPLGPLARWVSTPAELEGARAVILPGSKSTAADLAW
CCCCCCCCEEEECCCCCCCCCHHHCCCHHHHHHHCCCHHCCCCEEEEECCCCCCHHHHHH
LRASGLAGAITRVAQAGVPVLGICGGLQMLGRRIRDPHGVEGGGEVPGLGLLDLETEFAP
HHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCC
GKTTRLTACTDAETGLRLHGYEIHHGQTVSGLGVQELVPELLWRSGNVRGTYLHGLLENP
CCCEEEEEECCCCCCCEEEEEEEECCCEECCCCHHHHHHHHHHCCCCCCEEEEHHHHCCH
AYLERFLTWAGLTPPVPLASLDARLDAIAERVRVSLDWDFVRRLL
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEEECHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]