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The map label for this gene is insB [H]

Identifier: 94972261

GI number: 94972261

Start: 228937

End: 229383

Strand: Direct

Name: insB [H]

Synonym: Dgeo_2795

Alternate gene names: 94972261

Gene position: 228937-229383 (Clockwise)

Preceding gene: 94972264

Following gene: 94972259

Centisome position: 39.88

GC content: 61.3

Gene sequence:

>447_bases
GTGAGGCAGACCGTACCGGTCTGCCTCACACCTCCCGAAGAAGTGGTGGTTGAGCTGGATGAATTGTGGACCTTCGTGGG
CAAGAAAAAGCAGGCGAGGTGGCTCTGGATTGCCCTGGAGCGCAGCACCCGAAAGGTGCTGGCTTGGGTTCTGGGCGACC
GGAGTGAGCAAACCGCGTTCAAGCTCTGGGACCGCTTGCCGCTGTCCCCAGAACAGCGCCTGAAGGGCACGTTTTGCACG
GACCTGTGGCGTGCTTACGACGAGCCACTCCTGGGGGTGAAGCGGCTCACCCGCAAGGGGGAAACGAACCACGTCGAACG
GCTCAACTGCACCCTCAGACAGCGGCTAGGTCGGCTGGTCCGCAAGTCGTTGTCCTTCTCAAAGTCCGACGAGATGCTCG
AAGCGAGCCTGACCCTCGCCTTCCATCGCTACAACCTGTCACGTTGA

Upstream 100 bases:

>100_bases
TCCAGGCGGTTCATGAGCGAATGAGCCTCAGAGGCGTACAGCGCGTGTTTGGCGTCCACCGCAACACCGTCATCCGGTGG
ATAAAAAGGGGGCCTCCGAA

Downstream 100 bases:

>100_bases
TGCAGCCACTACCCAAGTTCTGAGTCAATCCCAAGATCCCCCCGGGTCAACAGTTCTCATACGCCAACGGTTTAGCAAAC
CGCTACAAAGATTGGGATTA

Product: IS1 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MRQTVPVCLTPPEEVVVELDELWTFVGKKKQARWLWIALERSTRKVLAWVLGDRSEQTAFKLWDRLPLSPEQRLKGTFCT
DLWRAYDEPLLGVKRLTRKGETNHVERLNCTLRQRLGRLVRKSLSFSKSDEMLEASLTLAFHRYNLSR

Sequences:

>Translated_148_residues
MRQTVPVCLTPPEEVVVELDELWTFVGKKKQARWLWIALERSTRKVLAWVLGDRSEQTAFKLWDRLPLSPEQRLKGTFCT
DLWRAYDEPLLGVKRLTRKGETNHVERLNCTLRQRLGRLVRKSLSFSKSDEMLEASLTLAFHRYNLSR
>Mature_148_residues
MRQTVPVCLTPPEEVVVELDELWTFVGKKKQARWLWIALERSTRKVLAWVLGDRSEQTAFKLWDRLPLSPEQRLKGTFCT
DLWRAYDEPLLGVKRLTRKGETNHVERLNCTLRQRLGRLVRKSLSFSKSDEMLEASLTLAFHRYNLSR

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1787223, Length=132, Percent_Identity=40.9090909090909, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1786470, Length=131, Percent_Identity=41.2213740458015, Blast_Score=80, Evalue=4e-17,
Organism=Escherichia coli, GI1786459, Length=131, Percent_Identity=41.2213740458015, Blast_Score=80, Evalue=4e-17,
Organism=Escherichia coli, GI1789853, Length=132, Percent_Identity=40.1515151515151, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1788203, Length=132, Percent_Identity=40.1515151515151, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1786203, Length=132, Percent_Identity=40.1515151515151, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 17442; Mature: 17442

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQTVPVCLTPPEEVVVELDELWTFVGKKKQARWLWIALERSTRKVLAWVLGDRSEQTAF
CCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHCCCCHHHHH
KLWDRLPLSPEQRLKGTFCTDLWRAYDEPLLGVKRLTRKGETNHVERLNCTLRQRLGRLV
HHHHCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RKSLSFSKSDEMLEASLTLAFHRYNLSR
HHHHCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRQTVPVCLTPPEEVVVELDELWTFVGKKKQARWLWIALERSTRKVLAWVLGDRSEQTAF
CCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHCCCCHHHHH
KLWDRLPLSPEQRLKGTFCTDLWRAYDEPLLGVKRLTRKGETNHVERLNCTLRQRLGRLV
HHHHCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RKSLSFSKSDEMLEASLTLAFHRYNLSR
HHHHCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]