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The map label for this gene is dosC [H]

Identifier: 94972207

GI number: 94972207

Start: 286482

End: 288149

Strand: Direct

Name: dosC [H]

Synonym: Dgeo_2740

Alternate gene names: 94972207

Gene position: 286482-288149 (Clockwise)

Preceding gene: 94972211

Following gene: 94972206

Centisome position: 49.9

GC content: 66.01

Gene sequence:

>1668_bases
GTGCTGGCACAGCTTCAGCGGCTGGAACGGCAGGAGGACCGGGCAGTGATCGCGTACCAGCAGGCGCTGATTTCTTGGGA
TGTGGAGGATCAGCGGGCGTACCAGGAGGCGCGGGAGGAATTCCACCGCCTCTGGCTGGCGGTCTATGCCAACGTGGGCG
ACAACCCGGTGCAGCAAGCGCGGCTGCGGGCATGGGACAACCACTTCAGAACCTGGGATGAGACGGTGGTGCAGCCCACC
CTGCGGACCAGCGGGACCAACGGCCTGCCCTCGCCGCCAGATCTGCGCCCGTTGCGTGCGAATGAGGCCATCGCCCGCGC
CCTGCACCAAACTCAGGAACAGCTGCTCGAGCAGCAGTGGACGGCCGCGCAGCACAGCACCCGCGTGGCGTACTGGGCGG
TGCTGTTGGGCCTCCCCCTGGCCGCACTGCTCACCTGGCGGCTGGGGCATAACCTGCTGCGCCGCATTCGCCAGGGCACC
CAGCCCCTGATTCTGGCGGCAGAACGGGTGATGGAAGGAGACCTCTCGGCCCGGGTACCGGTCAACGGCAATGATGAACT
CACGCGGCTAAGCCGCCGCTTCAACATGATGGCCAGCCGCCTCCAGAACGTGGTGAGTGAACAGCGGGAACTCCAGCACA
CCCTGGAGCAGCGGGTGGAACGGCTGGTACAGGACACCACCCGCGAGATTCAGCTTCTGGGACAGCTCGGCACCTTTATG
CAGGCCTGTCACGACGAGAGCGAGGCGTATGACGTGATCTGCCACACGGCGGAGGCGCTGTTTCCAGGAGGCGGGGAAAT
TGCGCTGCTGTCCGCCTCCCGCAACCTGCTCACGGTTCAGCTGCAGTGGGGCTTCGCGCTTCCTGGGCCCGAAGTCTTTG
GGCCAGAGGACTGCTGGGCGCTGCGCCTCGGGCAACCCCACGCGGCCCACGCGGGCCTGAGTCGCCCGCACTGCCAGCAT
GACACCTCAGGCCAGCCGACCCTCTGTGTGCCGCTGATAGCCCAGGGAGACACGCTGGGGGTGGTGACCTTGCACTTTGA
GACGGGAGAAGCACTCGAACTCGCGCGCCCGCTGGCCCAGCGCTTTGCGGAGCGGGTGGCCCTCTCGCTGGTGAATCTGC
GCCTGCGCGCCACACTGCGGCAGCAAAGCATCCGCGATCCCCTCACCGGGCTGTATAACCGGCGCTACCTCGAAGAAACC
TTTACACGCGAACTGGCACGGGTGCAGCGGCGCAGGCAGCCCCTCAGCCTGCTGATGATTGACGTGGACCATTTCAAGCA
GCACAACGACACCTACGGGCACGCCCTGGGAGACACGCTGCTGCGCCGCCTCGCCACCCACCTGCAGCGCTTCTTTCGCA
CAGAAGATATTGTCTGCCGTTACGGAGGCGAGGAATTCGTGGTGTTGCTGCCCGAATGTGACGCGCCGCAGGCCCAAGCC
CGCGCCGAGGCTCTGCGGCGTAGTGTGGAAAATCTGGCGGTCACCTCCAATGACGCAGAGTTCGTCGCCCTCACGATCTC
TATCGGCGGGGCGAGCTGGCCCGATCACGCCACCACGCCCGAGGATCTGCTGGCACGCGCTGACGCCGCCCTCTACCGTG
CCAAACACCGTGGCCGCAACCGGGTCGAATTCGAGCCAATTCCAGGAGCCGCAGGCCTGCCTGATTAA

Upstream 100 bases:

>100_bases
TGTCCTGCCGATTGTCTGTCTGGGCGTAATCATGGTGCTGACCCTCTCGGCCTTTCGGGCTTCACAACAAGGGACAGCGC
TGATCTCACACACAAGCGAT

Downstream 100 bases:

>100_bases
GCCTCTCCCCTCTCCCGCACCGGGGCGGCCTCCTAAATCGGCAACAGGCAGGAGAAGGAGAATCGTCATGAGGAATCAGG
TACCCTCAGGGCATGCATCC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MLAQLQRLERQEDRAVIAYQQALISWDVEDQRAYQEAREEFHRLWLAVYANVGDNPVQQARLRAWDNHFRTWDETVVQPT
LRTSGTNGLPSPPDLRPLRANEAIARALHQTQEQLLEQQWTAAQHSTRVAYWAVLLGLPLAALLTWRLGHNLLRRIRQGT
QPLILAAERVMEGDLSARVPVNGNDELTRLSRRFNMMASRLQNVVSEQRELQHTLEQRVERLVQDTTREIQLLGQLGTFM
QACHDESEAYDVICHTAEALFPGGGEIALLSASRNLLTVQLQWGFALPGPEVFGPEDCWALRLGQPHAAHAGLSRPHCQH
DTSGQPTLCVPLIAQGDTLGVVTLHFETGEALELARPLAQRFAERVALSLVNLRLRATLRQQSIRDPLTGLYNRRYLEET
FTRELARVQRRRQPLSLLMIDVDHFKQHNDTYGHALGDTLLRRLATHLQRFFRTEDIVCRYGGEEFVVLLPECDAPQAQA
RAEALRRSVENLAVTSNDAEFVALTISIGGASWPDHATTPEDLLARADAALYRAKHRGRNRVEFEPIPGAAGLPD

Sequences:

>Translated_555_residues
MLAQLQRLERQEDRAVIAYQQALISWDVEDQRAYQEAREEFHRLWLAVYANVGDNPVQQARLRAWDNHFRTWDETVVQPT
LRTSGTNGLPSPPDLRPLRANEAIARALHQTQEQLLEQQWTAAQHSTRVAYWAVLLGLPLAALLTWRLGHNLLRRIRQGT
QPLILAAERVMEGDLSARVPVNGNDELTRLSRRFNMMASRLQNVVSEQRELQHTLEQRVERLVQDTTREIQLLGQLGTFM
QACHDESEAYDVICHTAEALFPGGGEIALLSASRNLLTVQLQWGFALPGPEVFGPEDCWALRLGQPHAAHAGLSRPHCQH
DTSGQPTLCVPLIAQGDTLGVVTLHFETGEALELARPLAQRFAERVALSLVNLRLRATLRQQSIRDPLTGLYNRRYLEET
FTRELARVQRRRQPLSLLMIDVDHFKQHNDTYGHALGDTLLRRLATHLQRFFRTEDIVCRYGGEEFVVLLPECDAPQAQA
RAEALRRSVENLAVTSNDAEFVALTISIGGASWPDHATTPEDLLARADAALYRAKHRGRNRVEFEPIPGAAGLPD
>Mature_555_residues
MLAQLQRLERQEDRAVIAYQQALISWDVEDQRAYQEAREEFHRLWLAVYANVGDNPVQQARLRAWDNHFRTWDETVVQPT
LRTSGTNGLPSPPDLRPLRANEAIARALHQTQEQLLEQQWTAAQHSTRVAYWAVLLGLPLAALLTWRLGHNLLRRIRQGT
QPLILAAERVMEGDLSARVPVNGNDELTRLSRRFNMMASRLQNVVSEQRELQHTLEQRVERLVQDTTREIQLLGQLGTFM
QACHDESEAYDVICHTAEALFPGGGEIALLSASRNLLTVQLQWGFALPGPEVFGPEDCWALRLGQPHAAHAGLSRPHCQH
DTSGQPTLCVPLIAQGDTLGVVTLHFETGEALELARPLAQRFAERVALSLVNLRLRATLRQQSIRDPLTGLYNRRYLEET
FTRELARVQRRRQPLSLLMIDVDHFKQHNDTYGHALGDTLLRRLATHLQRFFRTEDIVCRYGGEEFVVLLPECDAPQAQA
RAEALRRSVENLAVTSNDAEFVALTISIGGASWPDHATTPEDLLARADAALYRAKHRGRNRVEFEPIPGAAGLPD

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=45.5696202531646, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=39.7515527950311, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=36.969696969697, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=38.2352941176471, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI87081881, Length=188, Percent_Identity=38.8297872340425, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1787802, Length=174, Percent_Identity=34.4827586206897, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=36.4705882352941, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788381, Length=186, Percent_Identity=35.4838709677419, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=32.5153374233129, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788956, Length=169, Percent_Identity=34.3195266272189, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=120, Percent_Identity=34.1666666666667, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=28.9156626506024, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=186, Percent_Identity=26.3440860215054, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 62721; Mature: 62721

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAQLQRLERQEDRAVIAYQQALISWDVEDQRAYQEAREEFHRLWLAVYANVGDNPVQQA
CCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RLRAWDNHFRTWDETVVQPTLRTSGTNGLPSPPDLRPLRANEAIARALHQTQEQLLEQQW
HHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TAAQHSTRVAYWAVLLGLPLAALLTWRLGHNLLRRIRQGTQPLILAAERVMEGDLSARVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEECC
VNGNDELTRLSRRFNMMASRLQNVVSEQRELQHTLEQRVERLVQDTTREIQLLGQLGTFM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QACHDESEAYDVICHTAEALFPGGGEIALLSASRNLLTVQLQWGFALPGPEVFGPEDCWA
HHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCEE
LRLGQPHAAHAGLSRPHCQHDTSGQPTLCVPLIAQGDTLGVVTLHFETGEALELARPLAQ
EECCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEEEECCCCHHHHHHHHHH
RFAERVALSLVNLRLRATLRQQSIRDPLTGLYNRRYLEETFTRELARVQRRRQPLSLLMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DVDHFKQHNDTYGHALGDTLLRRLATHLQRFFRTEDIVCRYGGEEFVVLLPECDAPQAQA
EHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCEEEEEECCCCCCHHHH
RAEALRRSVENLAVTSNDAEFVALTISIGGASWPDHATTPEDLLARADAALYRAKHRGRN
HHHHHHHHHHHHEEECCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
RVEFEPIPGAAGLPD
CCEECCCCCCCCCCC
>Mature Secondary Structure
MLAQLQRLERQEDRAVIAYQQALISWDVEDQRAYQEAREEFHRLWLAVYANVGDNPVQQA
CCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RLRAWDNHFRTWDETVVQPTLRTSGTNGLPSPPDLRPLRANEAIARALHQTQEQLLEQQW
HHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TAAQHSTRVAYWAVLLGLPLAALLTWRLGHNLLRRIRQGTQPLILAAERVMEGDLSARVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEECC
VNGNDELTRLSRRFNMMASRLQNVVSEQRELQHTLEQRVERLVQDTTREIQLLGQLGTFM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QACHDESEAYDVICHTAEALFPGGGEIALLSASRNLLTVQLQWGFALPGPEVFGPEDCWA
HHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCEE
LRLGQPHAAHAGLSRPHCQHDTSGQPTLCVPLIAQGDTLGVVTLHFETGEALELARPLAQ
EECCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEEEECCCCHHHHHHHHHH
RFAERVALSLVNLRLRATLRQQSIRDPLTGLYNRRYLEETFTRELARVQRRRQPLSLLMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DVDHFKQHNDTYGHALGDTLLRRLATHLQRFFRTEDIVCRYGGEEFVVLLPECDAPQAQA
EHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCEEEEEECCCCCCHHHH
RAEALRRSVENLAVTSNDAEFVALTISIGGASWPDHATTPEDLLARADAALYRAKHRGRN
HHHHHHHHHHHHEEECCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
RVEFEPIPGAAGLPD
CCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]