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The map label for this gene is cobB [H]

Identifier: 94971818

GI number: 94971818

Start: 144094

End: 145419

Strand: Direct

Name: cobB [H]

Synonym: Dgeo_2349

Alternate gene names: 94971818

Gene position: 144094-145419 (Clockwise)

Preceding gene: 94971819

Following gene: 94971817

Centisome position: 25.1

GC content: 72.02

Gene sequence:

>1326_bases
GTGAACCGTCTCGTCCTCGCCGCGCCGCACTCGGGGAGCGGCAAGACCACCGTGGCGTCCCTGCTCTGCCTCGCGCTGCG
GCAGCGTGGCCTTCGTGTGGCCCCCTTCAAGTTGGGGCCGGACTACCTCGACCCCACCCACCTCACGCGCGCGGCGGGGC
AACCCGCGCGCAACCTCGACTCGTTCCTGCTCACCCCGGAGCGGCTGCGCGAGCTGTTTGTGCGTGCCGCTGCCCAGGCC
GACATCAGCGTGCTAGAAGGCGTGATGGGCCTGTACGACGGCCGCGACCCCGCCAGCGACGCCCATTCCACCGCCGAGCT
CGCTCGCTTGCTCCAGGCCCCGGTGGTGCTGGTGATCGACGCGGGCGGCAGCGCGCGGACGGTGGCGGCGGTCGCGCACG
GCCTGCGTTCCTTTGGTCCAGACCTGAACGTCGTGGGCGTCATCCTCAACCGGGTGGCGGGCGAGCGGCACGCCTCACTT
TGCGAGGTGGCGCTCGCCCAGATTGGGCTGCCGGTGCTGGGCTTTGTTCCCCGCGACGAGGCCCTGCACCTTCCCGCCCG
GCACCTGGGCCTGCTGAGCGCCGAGCAGGCGAGCTGGAACGAGGCGGAGGCGCTCAAGGCCGCCGCGCATCTGCGCCTGG
ACACCCTGCTGGCCGCTGCCCAGGCTCCGCCGCTGCCCCTCCCGGCTCCGGCTCCGCGCCGCCCTGCGCGCCGCGTTCGC
ATCGCCTACGCCCTCGACGAGGCCTTTCACTTCTACTATCCCGACGCGCTCGATGCCCTACAAGAGGCGGGCGCGGAACT
CCTGCCCTTTAGTCCGCTGCGCGACGCGGGCTTGCCGCCGGACGTGGGGGGGGTACTGTTGGGGGGCGGCTATCCCGAAG
CGCATGCGGATGAACTCAGCGCCAACACAGCGATGCGGGCAGCCCTGCGGTCCTTTGCAGCGTCCGGGCGTCCCGTGATC
GGGGAATGCGGCGGCCTGATGTACCTGGGGGAGACGCTGGAGGACACCGCAGGCCGCCTCTTTGAGATGTGCGGCGTGAT
CCCCTACCGCACGCGGATGGCCCCCCGCCTCACGCTCGGCTACCGCGACGCCACCGTGCTCACCGACTCGCCGCTGGCCC
CGGCGGGGACGCTGCTGCGCGGTCACGAGTTCCACCACAGCGTGCTCACCCACGCGCCCACGCACCCGGCCTATGCCTGG
ACGGACTCCGGGGGCCGGCGGGTAGAGGAGGGCTACGCGCGCGGCCACGTGCTTGCGAGTTACCTACACCTGCATTGGGG
GGCTTCGGCCCAACTCGCCGAGCGGCTGGTGGAGGCGTGCTCGTGA

Upstream 100 bases:

>100_bases
CCTTGGTTGTGGATACAGAGACCGGTCATGTGCGCCTGGGCCGCGCGGCGCAGCTGGCCCAGGCTCTGGGCGCCGCAGTG
ACCACCCTGGGAGGCCCGCA

Downstream 100 bases:

>100_bases
GGCGGCCTGCACTGCTCTTGGCGCTTGTGCTGGACGCGCTGGGCGAACCTCCCGCGCGGCTGCATCCCGTGGTGGGGATG
GGGACCTTTCTGAAGGCGGC

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MNRLVLAAPHSGSGKTTVASLLCLALRQRGLRVAPFKLGPDYLDPTHLTRAAGQPARNLDSFLLTPERLRELFVRAAAQA
DISVLEGVMGLYDGRDPASDAHSTAELARLLQAPVVLVIDAGGSARTVAAVAHGLRSFGPDLNVVGVILNRVAGERHASL
CEVALAQIGLPVLGFVPRDEALHLPARHLGLLSAEQASWNEAEALKAAAHLRLDTLLAAAQAPPLPLPAPAPRRPARRVR
IAYALDEAFHFYYPDALDALQEAGAELLPFSPLRDAGLPPDVGGVLLGGGYPEAHADELSANTAMRAALRSFAASGRPVI
GECGGLMYLGETLEDTAGRLFEMCGVIPYRTRMAPRLTLGYRDATVLTDSPLAPAGTLLRGHEFHHSVLTHAPTHPAYAW
TDSGGRRVEEGYARGHVLASYLHLHWGASAQLAERLVEACS

Sequences:

>Translated_441_residues
MNRLVLAAPHSGSGKTTVASLLCLALRQRGLRVAPFKLGPDYLDPTHLTRAAGQPARNLDSFLLTPERLRELFVRAAAQA
DISVLEGVMGLYDGRDPASDAHSTAELARLLQAPVVLVIDAGGSARTVAAVAHGLRSFGPDLNVVGVILNRVAGERHASL
CEVALAQIGLPVLGFVPRDEALHLPARHLGLLSAEQASWNEAEALKAAAHLRLDTLLAAAQAPPLPLPAPAPRRPARRVR
IAYALDEAFHFYYPDALDALQEAGAELLPFSPLRDAGLPPDVGGVLLGGGYPEAHADELSANTAMRAALRSFAASGRPVI
GECGGLMYLGETLEDTAGRLFEMCGVIPYRTRMAPRLTLGYRDATVLTDSPLAPAGTLLRGHEFHHSVLTHAPTHPAYAW
TDSGGRRVEEGYARGHVLASYLHLHWGASAQLAERLVEACS
>Mature_441_residues
MNRLVLAAPHSGSGKTTVASLLCLALRQRGLRVAPFKLGPDYLDPTHLTRAAGQPARNLDSFLLTPERLRELFVRAAAQA
DISVLEGVMGLYDGRDPASDAHSTAELARLLQAPVVLVIDAGGSARTVAAVAHGLRSFGPDLNVVGVILNRVAGERHASL
CEVALAQIGLPVLGFVPRDEALHLPARHLGLLSAEQASWNEAEALKAAAHLRLDTLLAAAQAPPLPLPAPAPRRPARRVR
IAYALDEAFHFYYPDALDALQEAGAELLPFSPLRDAGLPPDVGGVLLGGGYPEAHADELSANTAMRAALRSFAASGRPVI
GECGGLMYLGETLEDTAGRLFEMCGVIPYRTRMAPRLTLGYRDATVLTDSPLAPAGTLLRGHEFHHSVLTHAPTHPAYAW
TDSGGRRVEEGYARGHVLASYLHLHWGASAQLAERLVEACS

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 46879; Mature: 46879

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLVLAAPHSGSGKTTVASLLCLALRQRGLRVAPFKLGPDYLDPTHLTRAAGQPARNLD
CCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEECEECCCCCCCHHHHHHHCCCCHHHHH
SFLLTPERLRELFVRAAAQADISVLEGVMGLYDGRDPASDAHSTAELARLLQAPVVLVID
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEE
AGGSARTVAAVAHGLRSFGPDLNVVGVILNRVAGERHASLCEVALAQIGLPVLGFVPRDE
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEECCCCCC
ALHLPARHLGLLSAEQASWNEAEALKAAAHLRLDTLLAAAQAPPLPLPAPAPRRPARRVR
CCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEE
IAYALDEAFHFYYPDALDALQEAGAELLPFSPLRDAGLPPDVGGVLLGGGYPEAHADELS
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHH
ANTAMRAALRSFAASGRPVIGECGGLMYLGETLEDTAGRLFEMCGVIPYRTRMAPRLTLG
HHHHHHHHHHHHHHCCCCCCHHCCCEEEECHHHHHHHHHHHHHHCCCCHHHCCCCEEEEC
YRDATVLTDSPLAPAGTLLRGHEFHHSVLTHAPTHPAYAWTDSGGRRVEEGYARGHVLAS
CCCCEEEECCCCCCCHHHHHCHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHH
YLHLHWGASAQLAERLVEACS
HHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNRLVLAAPHSGSGKTTVASLLCLALRQRGLRVAPFKLGPDYLDPTHLTRAAGQPARNLD
CCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEECEECCCCCCCHHHHHHHCCCCHHHHH
SFLLTPERLRELFVRAAAQADISVLEGVMGLYDGRDPASDAHSTAELARLLQAPVVLVID
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEE
AGGSARTVAAVAHGLRSFGPDLNVVGVILNRVAGERHASLCEVALAQIGLPVLGFVPRDE
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEECCCCCC
ALHLPARHLGLLSAEQASWNEAEALKAAAHLRLDTLLAAAQAPPLPLPAPAPRRPARRVR
CCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEE
IAYALDEAFHFYYPDALDALQEAGAELLPFSPLRDAGLPPDVGGVLLGGGYPEAHADELS
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHH
ANTAMRAALRSFAASGRPVIGECGGLMYLGETLEDTAGRLFEMCGVIPYRTRMAPRLTLG
HHHHHHHHHHHHHHCCCCCCHHCCCEEEECHHHHHHHHHHHHHHCCCCHHHCCCCEEEEC
YRDATVLTDSPLAPAGTLLRGHEFHHSVLTHAPTHPAYAWTDSGGRRVEEGYARGHVLAS
CCCCEEEECCCCCCCHHHHHCHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHH
YLHLHWGASAQLAERLVEACS
HHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]