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The map label for this gene is wcaG [H]

Identifier: 94314778

GI number: 94314778

Start: 55457

End: 56458

Strand: Reverse

Name: wcaG [H]

Synonym: Rmet_5859

Alternate gene names: 94314778

Gene position: 56458-55457 (Counterclockwise)

Preceding gene: 94314779

Following gene: 94314777

Centisome position: 2.19

GC content: 65.77

Gene sequence:

>1002_bases
ATGACTACCATGAGTGACGCACGCCCGAGAGTGTTCGTGGCCGGCCATCGCGGCATGGTCGGTTCCGCGATCGTGCGCGC
GCTGGCCGCGCGTGGCGACGTGGAACTGGTCCTGCGCACCCACCGCGAACTGGATCTGACCGAACAGCGGGACGTGCGCC
ATTTCTTCGCCACCGAACGGATCTCCCAGGTCTACCTGGCGGCCGCGCGCGTGGGCGGCATCCATGCCAATAACGTCTAT
CCCGCCGAGTTCATCCAGCAGAACCTGACGATCGCGGCCAACGTGATTCACGAAGCCTGGCGCGGCGGTGTCGAGCAGTT
GCTGTTCCTGGGTTCGAGTTGCATCTATCCGAAGTTCGCGTCGCAGCCCATCCAGGAATCGGCGTTGCTGACCGGCGCGC
TCGAGCCCACCAACGCGCCATACGCGATCGCCAAGATCGCCGGCATGATGATGTGCGACAGCTACAACCGCCAATACGGC
GTCGACTACCGGAGCGTGATGCCCACGAACCTCTATGGTCCGGGCGACAACTACCATCCCGAGAACAGCCACGTCATCCC
GGCATTGATCCGACGCATCCACGAGGCCAGGCTGGCGGATGCGCCGGAGGTCGCCGTGTGGGGCTCCGGTGCGCCGATGC
GCGAATTCCTTCACGCCGACGACCTCGCCCACGCCTGCCTGCATGTGATGGACCTGCCCCAGGCATCGTACCGGGCGCAT
ACGCAGGGCGCCGGATTCCTGAACGTCGGGAGCGGCGAAGAAGTCTCGATTCGACAGCTGGCGCTGCTGATCGCGCGCGT
GGTCGGCTATCGCGGCAACATCGCGTTCGACCCGACGCGCCCAGACGGCACGCCACGCAAGATGCTCGACTGCGGCCTTA
TCAACGCAACCGGCTGGCAGCCGCAGGTGTCGCTGGAATCCGGCCTGCGATCGACCTACCAGGAGGCGCTTTCGAGCGGA
CGGCTCAGCGTCGAGCCGGAAGCGCTGGCCACGCCAACCTAG

Upstream 100 bases:

>100_bases
CCACGCATCACGTTCGACGAACTAGTGCACGAAATGATCGAAGCGGACTTCGTGGCCGCGCGGCGTGACGCGCTGGTCAA
GATGGCCGGCTTCAGAACCT

Downstream 100 bases:

>100_bases
CGGTTCGAACGAAGCCAAGCGACTCCCGGGAGACCAACATGCGTCTGCAGCGCAACTTCGTATGGGACACCCTGCTGGCG
GCGACGCTGTTGCTGATCGT

Product: bifunctional GDP-4-dehydro-6-deoxy-D-mannose epimerase/GDP-4-dehydro-6-L-deoxygalactose reductase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTTMSDARPRVFVAGHRGMVGSAIVRALAARGDVELVLRTHRELDLTEQRDVRHFFATERISQVYLAAARVGGIHANNVY
PAEFIQQNLTIAANVIHEAWRGGVEQLLFLGSSCIYPKFASQPIQESALLTGALEPTNAPYAIAKIAGMMMCDSYNRQYG
VDYRSVMPTNLYGPGDNYHPENSHVIPALIRRIHEARLADAPEVAVWGSGAPMREFLHADDLAHACLHVMDLPQASYRAH
TQGAGFLNVGSGEEVSIRQLALLIARVVGYRGNIAFDPTRPDGTPRKMLDCGLINATGWQPQVSLESGLRSTYQEALSSG
RLSVEPEALATPT

Sequences:

>Translated_333_residues
MTTMSDARPRVFVAGHRGMVGSAIVRALAARGDVELVLRTHRELDLTEQRDVRHFFATERISQVYLAAARVGGIHANNVY
PAEFIQQNLTIAANVIHEAWRGGVEQLLFLGSSCIYPKFASQPIQESALLTGALEPTNAPYAIAKIAGMMMCDSYNRQYG
VDYRSVMPTNLYGPGDNYHPENSHVIPALIRRIHEARLADAPEVAVWGSGAPMREFLHADDLAHACLHVMDLPQASYRAH
TQGAGFLNVGSGEEVSIRQLALLIARVVGYRGNIAFDPTRPDGTPRKMLDCGLINATGWQPQVSLESGLRSTYQEALSSG
RLSVEPEALATPT
>Mature_332_residues
TTMSDARPRVFVAGHRGMVGSAIVRALAARGDVELVLRTHRELDLTEQRDVRHFFATERISQVYLAAARVGGIHANNVYP
AEFIQQNLTIAANVIHEAWRGGVEQLLFLGSSCIYPKFASQPIQESALLTGALEPTNAPYAIAKIAGMMMCDSYNRQYGV
DYRSVMPTNLYGPGDNYHPENSHVIPALIRRIHEARLADAPEVAVWGSGAPMREFLHADDLAHACLHVMDLPQASYRAHT
QGAGFLNVGSGEEVSIRQLALLIARVVGYRGNIAFDPTRPDGTPRKMLDCGLINATGWQPQVSLESGLRSTYQEALSSGR
LSVEPEALATPT

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=282, Percent_Identity=31.5602836879433, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI7657641, Length=287, Percent_Identity=25.0871080139373, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI42516563, Length=326, Percent_Identity=25.1533742331288, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788365, Length=314, Percent_Identity=63.3757961783439, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17554428, Length=281, Percent_Identity=30.6049822064057, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922778, Length=286, Percent_Identity=31.8181818181818, Blast_Score=148, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 36373; Mature: 36242

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMSDARPRVFVAGHRGMVGSAIVRALAARGDVELVLRTHRELDLTEQRDVRHFFATER
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
ISQVYLAAARVGGIHANNVYPAEFIQQNLTIAANVIHEAWRGGVEQLLFLGSSCIYPKFA
HHHHHHHHHHHCCEECCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
SQPIQESALLTGALEPTNAPYAIAKIAGMMMCDSYNRQYGVDYRSVMPTNLYGPGDNYHP
CCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCC
ENSHVIPALIRRIHEARLADAPEVAVWGSGAPMREFLHADDLAHACLHVMDLPQASYRAH
CCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
TQGAGFLNVGSGEEVSIRQLALLIARVVGYRGNIAFDPTRPDGTPRKMLDCGLINATGWQ
CCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCCCCCCCC
PQVSLESGLRSTYQEALSSGRLSVEPEALATPT
CCHHHHHHHHHHHHHHHHCCCCEECCHHCCCCC
>Mature Secondary Structure 
TTMSDARPRVFVAGHRGMVGSAIVRALAARGDVELVLRTHRELDLTEQRDVRHFFATER
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
ISQVYLAAARVGGIHANNVYPAEFIQQNLTIAANVIHEAWRGGVEQLLFLGSSCIYPKFA
HHHHHHHHHHHCCEECCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
SQPIQESALLTGALEPTNAPYAIAKIAGMMMCDSYNRQYGVDYRSVMPTNLYGPGDNYHP
CCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCC
ENSHVIPALIRRIHEARLADAPEVAVWGSGAPMREFLHADDLAHACLHVMDLPQASYRAH
CCCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
TQGAGFLNVGSGEEVSIRQLALLIARVVGYRGNIAFDPTRPDGTPRKMLDCGLINATGWQ
CCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCCCCCCCC
PQVSLESGLRSTYQEALSSGRLSVEPEALATPT
CCHHHHHHHHHHHHHHHHCCCCEECCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]