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The map label for this gene is gmd [H]

Identifier: 94314779

GI number: 94314779

Start: 56442

End: 57647

Strand: Reverse

Name: gmd [H]

Synonym: Rmet_5860

Alternate gene names: 94314779

Gene position: 57647-56442 (Counterclockwise)

Preceding gene: 94314780

Following gene: 94314778

Centisome position: 2.23

GC content: 63.27

Gene sequence:

>1206_bases
ATGGGCGTAAACGAACTGGATAACCTCTTCGACGCGGCCGACAAGCCCGGCGATGCATCGACGCCACGCGGCGCCAATGG
CCGGCGCCGGGCACTGATCACCGGCATCACCGGCCAGGACGGTTCGTACCTGTGCGAATACCTGCTCGACAAGGGATACG
AAGTCCACGGCATCAAGCGGCGCAGTTCGCTGTTCAATACCGAGCGTATCGATCATCTGTACGAGGACCCGCAAGCACCG
GACCGCAGGTTGATCCTCCATCACGGCGACATGACCGATACGGCGAGCCTCGTGCGCGTCGTGCAACAGTCCCGCCCCGA
CGAGGTCTACAACCTCGCGGCGCAGAGCCACGTGCAGGTGTCCTTCGAGGAGCCCGAGTACACCGCCAACACCGACGCCG
TCGGCACGCTGCGCCTGCTCGAGGCGATGCGGATTCTCGGCATGGAGCAAAGCACGCGTTTCTACCAGGCATCCACGTCC
GAACTGTATGGACTGGTGCAGGAGATTCCGCAGAAGGAAACCACGCCGTTCTATCCGCGCAGCCCGTATGCCGTGGCCAA
GCTCTACGCCTACTGGATCACCGTGAACTACCGTGAAGCCTATGGCATGTACGCGTGCAACGGCATCCTGTTCAATCACG
AAAGCCCCGTGCGCGGCGAGACGTTCGTCACCCGCAAGATCACACGCGCCATCGCGCGCATCGCGCTGGGCCTGCAGGAA
GTGCTCTATCTTGGCAACCTGTCCGCGCTGCGCGACTGGGGCCATGCGCGCGACTACGTCGAGATGCAGTGGCTGATGCT
GCAGCAGGAAACGCCGCGTGATTTCGTCATCGCCACGGGCGAGCAACACAGCGTGCGCGAGTTCGTCACGCTCTCCGCGC
AGAAGCTCGGGCTAACGGTCACCTTCGAGGGCAGCGGCGTCGACGAGGTCGGCATCATTTCTCATATCGACACCTCACGC
CGCGAGTTGTCCTCCCGCTGCAAGGTCGGCGACGTGATCGTGCGCGTGGACCCGCGCTACTTCCGCCCGACCGAAGTCGA
GACGCTGCTGGGCGACCCGACGCAGGCACGTGAGCAACTGGGCTGGTCCCCACGCATCACGTTCGACGAACTAGTGCACG
AAATGATCGAAGCGGACTTCGTGGCCGCGCGGCGTGACGCGCTGGTCAAGATGGCCGGCTTCAGAACCTATGACTACCAT
GAGTGA

Upstream 100 bases:

>100_bases
GCACGTCACGCCCGGGCGCTGCCACTGTGTGGGGTAGCAGACAGTGGCTTCAGGACACCCCCCCTACAGTCCGCGTATTG
CTAACCAAAATGTGACTGTC

Downstream 100 bases:

>100_bases
CGCACGCCCGAGAGTGTTCGTGGCCGGCCATCGCGGCATGGTCGGTTCCGCGATCGTGCGCGCGCTGGCCGCGCGTGGCG
ACGTGGAACTGGTCCTGCGC

Product: GDP-D-mannose dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MGVNELDNLFDAADKPGDASTPRGANGRRRALITGITGQDGSYLCEYLLDKGYEVHGIKRRSSLFNTERIDHLYEDPQAP
DRRLILHHGDMTDTASLVRVVQQSRPDEVYNLAAQSHVQVSFEEPEYTANTDAVGTLRLLEAMRILGMEQSTRFYQASTS
ELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYREAYGMYACNGILFNHESPVRGETFVTRKITRAIARIALGLQE
VLYLGNLSALRDWGHARDYVEMQWLMLQQETPRDFVIATGEQHSVREFVTLSAQKLGLTVTFEGSGVDEVGIISHIDTSR
RELSSRCKVGDVIVRVDPRYFRPTEVETLLGDPTQAREQLGWSPRITFDELVHEMIEADFVAARRDALVKMAGFRTYDYH
E

Sequences:

>Translated_401_residues
MGVNELDNLFDAADKPGDASTPRGANGRRRALITGITGQDGSYLCEYLLDKGYEVHGIKRRSSLFNTERIDHLYEDPQAP
DRRLILHHGDMTDTASLVRVVQQSRPDEVYNLAAQSHVQVSFEEPEYTANTDAVGTLRLLEAMRILGMEQSTRFYQASTS
ELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYREAYGMYACNGILFNHESPVRGETFVTRKITRAIARIALGLQE
VLYLGNLSALRDWGHARDYVEMQWLMLQQETPRDFVIATGEQHSVREFVTLSAQKLGLTVTFEGSGVDEVGIISHIDTSR
RELSSRCKVGDVIVRVDPRYFRPTEVETLLGDPTQAREQLGWSPRITFDELVHEMIEADFVAARRDALVKMAGFRTYDYH
E
>Mature_400_residues
GVNELDNLFDAADKPGDASTPRGANGRRRALITGITGQDGSYLCEYLLDKGYEVHGIKRRSSLFNTERIDHLYEDPQAPD
RRLILHHGDMTDTASLVRVVQQSRPDEVYNLAAQSHVQVSFEEPEYTANTDAVGTLRLLEAMRILGMEQSTRFYQASTSE
LYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYREAYGMYACNGILFNHESPVRGETFVTRKITRAIARIALGLQEV
LYLGNLSALRDWGHARDYVEMQWLMLQQETPRDFVIATGEQHSVREFVTLSAQKLGLTVTFEGSGVDEVGIISHIDTSRR
ELSSRCKVGDVIVRVDPRYFRPTEVETLLGDPTQAREQLGWSPRITFDELVHEMIEADFVAARRDALVKMAGFRTYDYHE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=375, Percent_Identity=59.7333333333333, Blast_Score=457, Evalue=1e-128,
Organism=Escherichia coli, GI1788366, Length=366, Percent_Identity=68.8524590163934, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI48994969, Length=240, Percent_Identity=29.5833333333333, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788353, Length=261, Percent_Identity=25.6704980842912, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786974, Length=359, Percent_Identity=25.3481894150418, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=363, Percent_Identity=61.7079889807163, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17539424, Length=364, Percent_Identity=60.1648351648352, Blast_Score=460, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI133901788, Length=364, Percent_Identity=60.1648351648352, Blast_Score=460, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI133901790, Length=364, Percent_Identity=60.1648351648352, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17539422, Length=364, Percent_Identity=60.1648351648352, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI133901786, Length=364, Percent_Identity=60.1648351648352, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24158427, Length=378, Percent_Identity=57.9365079365079, Blast_Score=461, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 45575; Mature: 45444

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00061 ADH_SHORT ; PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVNELDNLFDAADKPGDASTPRGANGRRRALITGITGQDGSYLCEYLLDKGYEVHGIKR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEHH
RSSLFNTERIDHLYEDPQAPDRRLILHHGDMTDTASLVRVVQQSRPDEVYNLAAQSHVQV
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCEEEE
SFEEPEYTANTDAVGTLRLLEAMRILGMEQSTRFYQASTSELYGLVQEIPQKETTPFYPR
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCC
SPYAVAKLYAYWITVNYREAYGMYACNGILFNHESPVRGETFVTRKITRAIARIALGLQE
CCHHHHEEEEEEEEEEHHHHCCEEEECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VLYLGNLSALRDWGHARDYVEMQWLMLQQETPRDFVIATGEQHSVREFVTLSAQKLGLTV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHEEEEE
TFEGSGVDEVGIISHIDTSRRELSSRCKVGDVIVRVDPRYFRPTEVETLLGDPTQAREQL
EECCCCCCCCHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHCCCCHHHHHHC
GWSPRITFDELVHEMIEADFVAARRDALVKMAGFRTYDYHE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GVNELDNLFDAADKPGDASTPRGANGRRRALITGITGQDGSYLCEYLLDKGYEVHGIKR
CCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEHH
RSSLFNTERIDHLYEDPQAPDRRLILHHGDMTDTASLVRVVQQSRPDEVYNLAAQSHVQV
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCEEEE
SFEEPEYTANTDAVGTLRLLEAMRILGMEQSTRFYQASTSELYGLVQEIPQKETTPFYPR
EECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCC
SPYAVAKLYAYWITVNYREAYGMYACNGILFNHESPVRGETFVTRKITRAIARIALGLQE
CCHHHHEEEEEEEEEEHHHHCCEEEECEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VLYLGNLSALRDWGHARDYVEMQWLMLQQETPRDFVIATGEQHSVREFVTLSAQKLGLTV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHEEEEE
TFEGSGVDEVGIISHIDTSRRELSSRCKVGDVIVRVDPRYFRPTEVETLLGDPTQAREQL
EECCCCCCCCHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHCCCCHHHHHHC
GWSPRITFDELVHEMIEADFVAARRDALVKMAGFRTYDYHE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]