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The map label for this gene is yafC [C]

Identifier: 94312821

GI number: 94312821

Start: 436925

End: 437878

Strand: Direct

Name: yafC [C]

Synonym: Rmet_3893

Alternate gene names: 94312821

Gene position: 436925-437878 (Clockwise)

Preceding gene: 94312818

Following gene: 94312822

Centisome position: 16.93

GC content: 66.35

Gene sequence:

>954_bases
ATGCACAGGCACTTCGACGACGTCCTGCTCGGCAGCATCGAGCTGTTTTGCCTCGCGGCCGAAGCCGGTAGCTTCACCGC
CGCTGCCAACACCGCCGGGGTCACGCCGGCGGCCGTAAGCCGCTCGATCTCACGGCTGGAGAAGCGCCTGGGCGTGCGCC
TCTTTGTGCGGTCCACGCGCAGCATCCGGCTGACCGAGGGCGGCAGGAGCTACTTCGAGCAATGCCGTGGCGCGCTCAAC
CAGTTGATCGAGGCCGAGCGCAAGGTCACGGGCGAGCAGGTCCAGCCTTCGGGCACCCTGCGTATCAGCGCGCCGACGAC
CTACGGGCACTACCGGCTGCTGCCCTTGCTGCCCGCCTTGCGCGAGCGGTTTCCTCTCCTCAGGTTCGAGGTCCAGATCG
GCAACCGCAATATCGATTTCCACGAGGAGAACTTCGATGTGGCGATCCGGTTCCGCGCGCAGCCGGATTCGACGATGGTC
TCGCGCCATCTGGAGGATGCGGAACTGGTGGTGGTGGCAAGCCCTGGGTATCTGAAGCGGGCCGGACGGCCGCAGGCCCT
GGAGGATTTGCAGCAGCACGAGTGCATTCAGTTCGATCTGCCCAGCAGTGGCCGGCCGATCGCGTGGCTGTTTATGGATG
GCGGCCGTGAACGGGAGGTGCTGGCCACGGAAAGCCATTATCTGTGCGCCGAGGACGTGCTTGCCGGCGTCACCCTGGCC
AGGTCTGGTGCCGGTCTGTTCCAAACCTACCGATTTATCGTCGAACGGGACTTGGCCGACGGCACGCTGGTCGAGGTCCT
GCAGCCATTTGCTGGCCGGTCACGGCCAGTCAGTCTGATGTACCCGCACGGGCGCCTGCTGCCGTCGAGGGTCCGGGTGT
TCGTGGATTTTCTGATGGAGCAGGCCGCGGCCCTGGCAAGCGCGCGGGGGGCGGGGAAGCCTGCCCGGGGCTAG

Upstream 100 bases:

>100_bases
GCGTTTGCAGTGCCGCCTCCGGTTGAACACACTCTATTCCGGCATCAAAAAGCAAAAAACGGGGTTATGTACACTTGTCC
TGTTACATGGAGTAATAAAT

Downstream 100 bases:

>100_bases
CGAATGAGCGAATGGCCGGGTGCCGGCTGTTGCGCAAGTGGCCGTGAAAAAAGCGTCGGCCGAACCTTGTCACCCTCCTT
TCTTAGGGAAATCGTCTCTA

Product: putative transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MHRHFDDVLLGSIELFCLAAEAGSFTAAANTAGVTPAAVSRSISRLEKRLGVRLFVRSTRSIRLTEGGRSYFEQCRGALN
QLIEAERKVTGEQVQPSGTLRISAPTTYGHYRLLPLLPALRERFPLLRFEVQIGNRNIDFHEENFDVAIRFRAQPDSTMV
SRHLEDAELVVVASPGYLKRAGRPQALEDLQQHECIQFDLPSSGRPIAWLFMDGGREREVLATESHYLCAEDVLAGVTLA
RSGAGLFQTYRFIVERDLADGTLVEVLQPFAGRSRPVSLMYPHGRLLPSRVRVFVDFLMEQAAALASARGAGKPARG

Sequences:

>Translated_317_residues
MHRHFDDVLLGSIELFCLAAEAGSFTAAANTAGVTPAAVSRSISRLEKRLGVRLFVRSTRSIRLTEGGRSYFEQCRGALN
QLIEAERKVTGEQVQPSGTLRISAPTTYGHYRLLPLLPALRERFPLLRFEVQIGNRNIDFHEENFDVAIRFRAQPDSTMV
SRHLEDAELVVVASPGYLKRAGRPQALEDLQQHECIQFDLPSSGRPIAWLFMDGGREREVLATESHYLCAEDVLAGVTLA
RSGAGLFQTYRFIVERDLADGTLVEVLQPFAGRSRPVSLMYPHGRLLPSRVRVFVDFLMEQAAALASARGAGKPARG
>Mature_317_residues
MHRHFDDVLLGSIELFCLAAEAGSFTAAANTAGVTPAAVSRSISRLEKRLGVRLFVRSTRSIRLTEGGRSYFEQCRGALN
QLIEAERKVTGEQVQPSGTLRISAPTTYGHYRLLPLLPALRERFPLLRFEVQIGNRNIDFHEENFDVAIRFRAQPDSTMV
SRHLEDAELVVVASPGYLKRAGRPQALEDLQQHECIQFDLPSSGRPIAWLFMDGGREREVLATESHYLCAEDVLAGVTLA
RSGAGLFQTYRFIVERDLADGTLVEVLQPFAGRSRPVSLMYPHGRLLPSRVRVFVDFLMEQAAALASARGAGKPARG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=33.448275862069, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1787128, Length=285, Percent_Identity=30.5263157894737, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=30.4054054054054, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1789639, Length=316, Percent_Identity=29.746835443038, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=31.8493150684932, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789440, Length=312, Percent_Identity=28.525641025641, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788706, Length=306, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=26.8041237113402, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786448, Length=298, Percent_Identity=26.510067114094, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789173, Length=188, Percent_Identity=32.9787234042553, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=27.6450511945392, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI157672245, Length=142, Percent_Identity=34.5070422535211, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082132, Length=296, Percent_Identity=24.6621621621622, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786508, Length=117, Percent_Identity=36.7521367521368, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35162; Mature: 35162

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRHFDDVLLGSIELFCLAAEAGSFTAAANTAGVTPAAVSRSISRLEKRLGVRLFVRSTR
CCCCHHHHHHCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEEECCC
SIRLTEGGRSYFEQCRGALNQLIEAERKVTGEQVQPSGTLRISAPTTYGHYRLLPLLPAL
EEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEEHHHHHHHH
RERFPLLRFEVQIGNRNIDFHEENFDVAIRFRAQPDSTMVSRHLEDAELVVVASPGYLKR
HHHCCEEEEEEEECCCCCCEECCCCEEEEEEECCCCHHHHHHCCCCCEEEEEECCCHHHH
AGRPQALEDLQQHECIQFDLPSSGRPIAWLFMDGGREREVLATESHYLCAEDVLAGVTLA
CCCCHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCEEEECCCCCEEHHHHHHHHHHH
RSGAGLFQTYRFIVERDLADGTLVEVLQPFAGRSRPVSLMYPHGRLLPSRVRVFVDFLME
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
QAAALASARGAGKPARG
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHRHFDDVLLGSIELFCLAAEAGSFTAAANTAGVTPAAVSRSISRLEKRLGVRLFVRSTR
CCCCHHHHHHCCEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEEECCC
SIRLTEGGRSYFEQCRGALNQLIEAERKVTGEQVQPSGTLRISAPTTYGHYRLLPLLPAL
EEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEEHHHHHHHH
RERFPLLRFEVQIGNRNIDFHEENFDVAIRFRAQPDSTMVSRHLEDAELVVVASPGYLKR
HHHCCEEEEEEEECCCCCCEECCCCEEEEEEECCCCHHHHHHCCCCCEEEEEECCCHHHH
AGRPQALEDLQQHECIQFDLPSSGRPIAWLFMDGGREREVLATESHYLCAEDVLAGVTLA
CCCCHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCEEEECCCCCEEHHHHHHHHHHH
RSGAGLFQTYRFIVERDLADGTLVEVLQPFAGRSRPVSLMYPHGRLLPSRVRVFVDFLME
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHH
QAAALASARGAGKPARG
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]