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The map label for this gene is fucA [C]

Identifier: 94310786

GI number: 94310786

Start: 2002000

End: 2002833

Strand: Reverse

Name: fucA [C]

Synonym: Rmet_1848

Alternate gene names: 94310786

Gene position: 2002833-2002000 (Counterclockwise)

Preceding gene: 94310787

Following gene: 94310785

Centisome position: 50.99

GC content: 65.47

Gene sequence:

>834_bases
ATGAATGCCCCGCAAGCCAAGGATGAGATCGGCGCCGGTCTCTCGGAAGAGGTCAAGGCCCGCTATCGCAACCTGCCGCG
CCCGCCCCAGTTCGCCACGCCGGCCGAGGAGCGCCTGCATCGCAAGCAGCGGCTGGCGGCAGCATTCCGGTTGTTCTCCA
AGTTCGGTTTCGACGAAGGCGTGGCCGGCCATATCACCGCGCGCGATCCCGAGTTTGGCGATACCTTCTGGGTCAATCCG
TTCGGCGTGCATTTCAGCCAGGTCAAGGTGTCGAACCTGATCCGCTGCGACCACCACGGTAACGTGGTGGAGGGCGACTA
TCCGGTCAACGCCGCCGCATTCGCGATCCACTCGCGCGTGCACCAGACCCGCTCGGATGCCGTGGCCGCCGCGCACTCGC
ACAGTACCTTTGGCCGTGCGTGGTCGACGCTCGGGCGCAAGCTCGATCCGCTGACGCAGGACGTCTGCGCCTTCTACAAC
GACCACGCGCTGTATGACGATTTCGGTGGCGTGGTGGTGGAGCTCGACGAAGGTCAGCGTATTGCCAACGCGCTAGGCGA
CAACAAGGCAGCGATCCTCCAGAACCACGGCCTCCTGACCGTCGGCAAGACCGTCGACGAAGCCGCGTGGTGGTTCATCA
CGATGGAGCGGTCGTGCCAGGTGCAGTTGCTGGCGGAGGCCGCGGCTGCGCGTACCGGTGACGTGCCGAAGGCGATCAGC
GACGCCGCCGCACGTCAGGCGTACTCGATCGTTGGTACGTCGCAGGCGGGGTGGTTCCAGTTCCAGCCGCTCTATGCGCG
CATCGTGAAGGAGCAGCCGGACCTGCTCGACTGA

Upstream 100 bases:

>100_bases
GAACTTCGGCAAGCAGCTCGTGAAGCTGGCATAAAGCCGCCTTCGACGCAGTACACCCCCGCAAATAGACAAATAGACAA
AGAAAGCACAGGAGACAACC

Downstream 100 bases:

>100_bases
TTCCAACGGGAGGCGCGGATGCCCGACATCATTCTTCATCAGTACGCCACGTCGCCTTTCTCGGAAAAGATCCGCCTGCT
GCTCGGCGCGAAGGATCTGT

Product: hypothetical protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNAPQAKDEIGAGLSEEVKARYRNLPRPPQFATPAEERLHRKQRLAAAFRLFSKFGFDEGVAGHITARDPEFGDTFWVNP
FGVHFSQVKVSNLIRCDHHGNVVEGDYPVNAAAFAIHSRVHQTRSDAVAAAHSHSTFGRAWSTLGRKLDPLTQDVCAFYN
DHALYDDFGGVVVELDEGQRIANALGDNKAAILQNHGLLTVGKTVDEAAWWFITMERSCQVQLLAEAAAARTGDVPKAIS
DAAARQAYSIVGTSQAGWFQFQPLYARIVKEQPDLLD

Sequences:

>Translated_277_residues
MNAPQAKDEIGAGLSEEVKARYRNLPRPPQFATPAEERLHRKQRLAAAFRLFSKFGFDEGVAGHITARDPEFGDTFWVNP
FGVHFSQVKVSNLIRCDHHGNVVEGDYPVNAAAFAIHSRVHQTRSDAVAAAHSHSTFGRAWSTLGRKLDPLTQDVCAFYN
DHALYDDFGGVVVELDEGQRIANALGDNKAAILQNHGLLTVGKTVDEAAWWFITMERSCQVQLLAEAAAARTGDVPKAIS
DAAARQAYSIVGTSQAGWFQFQPLYARIVKEQPDLLD
>Mature_277_residues
MNAPQAKDEIGAGLSEEVKARYRNLPRPPQFATPAEERLHRKQRLAAAFRLFSKFGFDEGVAGHITARDPEFGDTFWVNP
FGVHFSQVKVSNLIRCDHHGNVVEGDYPVNAAAFAIHSRVHQTRSDAVAAAHSHSTFGRAWSTLGRKLDPLTQDVCAFYN
DHALYDDFGGVVVELDEGQRIANALGDNKAAILQNHGLLTVGKTVDEAAWWFITMERSCQVQLLAEAAAARTGDVPKAIS
DAAARQAYSIVGTSQAGWFQFQPLYARIVKEQPDLLD

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI29826323, Length=198, Percent_Identity=31.8181818181818, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI29826325, Length=198, Percent_Identity=31.8181818181818, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI29826319, Length=197, Percent_Identity=31.9796954314721, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI29826321, Length=197, Percent_Identity=31.9796954314721, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI297307160, Length=232, Percent_Identity=28.448275862069, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI89276787, Length=232, Percent_Identity=28.448275862069, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI9257190, Length=232, Percent_Identity=28.448275862069, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI297307158, Length=197, Percent_Identity=29.4416243654822, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI9257192, Length=197, Percent_Identity=29.4416243654822, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI9943848, Length=198, Percent_Identity=28.2828282828283, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI9951927, Length=198, Percent_Identity=28.2828282828283, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI62912453, Length=198, Percent_Identity=28.2828282828283, Blast_Score=88, Evalue=7e-18,
Organism=Escherichia coli, GI1789164, Length=193, Percent_Identity=28.4974093264249, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115533216, Length=190, Percent_Identity=28.4210526315789, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115533218, Length=224, Percent_Identity=27.2321428571429, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71989541, Length=233, Percent_Identity=27.8969957081545, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71989560, Length=233, Percent_Identity=27.8969957081545, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71989550, Length=233, Percent_Identity=27.8969957081545, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71989555, Length=233, Percent_Identity=27.8969957081545, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71989544, Length=233, Percent_Identity=27.8969957081545, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281363755, Length=189, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281363751, Length=189, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24655690, Length=189, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281363763, Length=186, Percent_Identity=33.8709677419355, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281363757, Length=186, Percent_Identity=33.8709677419355, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17136738, Length=186, Percent_Identity=33.8709677419355, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24655685, Length=186, Percent_Identity=33.8709677419355, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281363761, Length=186, Percent_Identity=33.8709677419355, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281363759, Length=186, Percent_Identity=33.8709677419355, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281363753, Length=186, Percent_Identity=33.8709677419355, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 30485; Mature: 30485

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPQAKDEIGAGLSEEVKARYRNLPRPPQFATPAEERLHRKQRLAAAFRLFSKFGFDEG
CCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
VAGHITARDPEFGDTFWVNPFGVHFSQVKVSNLIRCDHHGNVVEGDYPVNAAAFAIHSRV
CCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHEEECCCCCEECCCCCCCHHHHHHHHHH
HQTRSDAVAAAHSHSTFGRAWSTLGRKLDPLTQDVCAFYNDHALYDDFGGVVVELDEGQR
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEECCCCEEEEECCCHH
IANALGDNKAAILQNHGLLTVGKTVDEAAWWFITMERSCQVQLLAEAAAARTGDVPKAIS
HHHHHCCCCEEEEECCCEEEECCCHHCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHH
DAAARQAYSIVGTSQAGWFQFQPLYARIVKEQPDLLD
HHHHHHHHHHEECCCCCCEEECHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNAPQAKDEIGAGLSEEVKARYRNLPRPPQFATPAEERLHRKQRLAAAFRLFSKFGFDEG
CCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
VAGHITARDPEFGDTFWVNPFGVHFSQVKVSNLIRCDHHGNVVEGDYPVNAAAFAIHSRV
CCCEEECCCCCCCCEEECCCCCCCHHHHHHHHHEEECCCCCEECCCCCCCHHHHHHHHHH
HQTRSDAVAAAHSHSTFGRAWSTLGRKLDPLTQDVCAFYNDHALYDDFGGVVVELDEGQR
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEECCCCEEEEECCCHH
IANALGDNKAAILQNHGLLTVGKTVDEAAWWFITMERSCQVQLLAEAAAARTGDVPKAIS
HHHHHCCCCEEEEECCCEEEECCCHHCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHH
DAAARQAYSIVGTSQAGWFQFQPLYARIVKEQPDLLD
HHHHHHHHHHEECCCCCCEEECHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]