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The map label for this gene is ycfH [C]

Identifier: 94310763

GI number: 94310763

Start: 1981363

End: 1982160

Strand: Reverse

Name: ycfH [C]

Synonym: Rmet_1825

Alternate gene names: 94310763

Gene position: 1982160-1981363 (Counterclockwise)

Preceding gene: 94310764

Following gene: 94310762

Centisome position: 50.46

GC content: 60.4

Gene sequence:

>798_bases
ATGTTTGTTGATTCCCACTGCCATATCGATTTTCCCGAGCTGGCCGAAAGGCTGCCGCAGTTGCTCGAGAACATGCGGAC
CAATCAGGTGTCGCACGCCCTCTGCATCTCGGTGACGCTCGAGGACTTTCCGCGCGTGCTGGCGCTGGCCGAACAGCATC
CGAACCTGTATGCCTCAGTCGGCGTGCATCCTGACTACGAGGAAGGCGCCGAAGAACCGACACTCGAGCGGCTGGTCACG
CTGTCGGCGCATCCGCGAGTGGTTGGTACCGGCGAGACCGGGCTGGACTACTACCGTCTCAATGGTCGCAGCATCGCGGA
GATGGAGTGGCAGCGTGAGCGGTTCCGCACCCATATCCGCGCGGCCCGCCAGACTGGCAAGCCGCTGATCATCCATACGC
GATCCTCGGCGGACGACACGCTGCGGCTGATGCGCGAGGAAAACGCAGGTGAGGTTGGCGGCGTCATGCACTGCTTCACG
GAGACCTGGGACATCGCCCGGCAGGCGCTGGACGAAGGCTTCCATATTTCGTTCTCGGGTATCGTCACGTTCAAGAGCGC
GGCTGACCTGCAAGAAACCGCGAAGAAGGTACCGCTCGACCGCATGCTGATCGAAACCGATTCGCCTTATCTTGCCCCGG
TGCCGTACCGCGGCAAGACGAACGAGCCGGCCTGGGTGCGTCACGTGGGCGAGTTCATCGCCAAGCTGCGCGATGTTCCC
GTGGAGACGATCGCAGAACAAACTACAGATAATTTTTTCCGCTTATTCAAGCACATAGACAGGAATTCTCATGCTTGA

Upstream 100 bases:

>100_bases
TGGTTTTGCCGCGGGTATCGTCGTTCGATGCCGGGCCGGTGACCGTGGCGTCGAGCGAGACCTGATGATCGCCCGACGTC
GGCTCGATTGAGATATCCCC

Downstream 100 bases:

>100_bases
CTTTAAGATCAGACGCGGGGTCGCCGCAATGGCCCTGATCTGCGCGGCTTGTGCGAGTGTGGCCGCTCCGGCCGACGATA
TGCGCAAGGCCGTCGAGTTC

Product: putative metallodependent hydrolase, TatD family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MFVDSHCHIDFPELAERLPQLLENMRTNQVSHALCISVTLEDFPRVLALAEQHPNLYASVGVHPDYEEGAEEPTLERLVT
LSAHPRVVGTGETGLDYYRLNGRSIAEMEWQRERFRTHIRAARQTGKPLIIHTRSSADDTLRLMREENAGEVGGVMHCFT
ETWDIARQALDEGFHISFSGIVTFKSAADLQETAKKVPLDRMLIETDSPYLAPVPYRGKTNEPAWVRHVGEFIAKLRDVP
VETIAEQTTDNFFRLFKHIDRNSHA

Sequences:

>Translated_265_residues
MFVDSHCHIDFPELAERLPQLLENMRTNQVSHALCISVTLEDFPRVLALAEQHPNLYASVGVHPDYEEGAEEPTLERLVT
LSAHPRVVGTGETGLDYYRLNGRSIAEMEWQRERFRTHIRAARQTGKPLIIHTRSSADDTLRLMREENAGEVGGVMHCFT
ETWDIARQALDEGFHISFSGIVTFKSAADLQETAKKVPLDRMLIETDSPYLAPVPYRGKTNEPAWVRHVGEFIAKLRDVP
VETIAEQTTDNFFRLFKHIDRNSHA
>Mature_265_residues
MFVDSHCHIDFPELAERLPQLLENMRTNQVSHALCISVTLEDFPRVLALAEQHPNLYASVGVHPDYEEGAEEPTLERLVT
LSAHPRVVGTGETGLDYYRLNGRSIAEMEWQRERFRTHIRAARQTGKPLIIHTRSSADDTLRLMREENAGEVGGVMHCFT
ETWDIARQALDEGFHISFSGIVTFKSAADLQETAKKVPLDRMLIETDSPYLAPVPYRGKTNEPAWVRHVGEFIAKLRDVP
VETIAEQTTDNFFRLFKHIDRNSHA

Specific function: Unknown

COG id: COG0084

COG function: function code L; Mg-dependent DNase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tatD DNase family [H]

Homologues:

Organism=Homo sapiens, GI110349730, Length=270, Percent_Identity=27.4074074074074, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI110349734, Length=270, Percent_Identity=27.4074074074074, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI226061853, Length=275, Percent_Identity=27.6363636363636, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI226061614, Length=263, Percent_Identity=27.3764258555133, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI225903424, Length=270, Percent_Identity=27.037037037037, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI225903439, Length=246, Percent_Identity=26.8292682926829, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI14042943, Length=246, Percent_Identity=26.8292682926829, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI226061595, Length=229, Percent_Identity=27.0742358078603, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787342, Length=263, Percent_Identity=46.0076045627376, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI87082439, Length=258, Percent_Identity=29.4573643410853, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI48994985, Length=232, Percent_Identity=29.7413793103448, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17565396, Length=307, Percent_Identity=28.0130293159609, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17559024, Length=284, Percent_Identity=26.056338028169, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17543026, Length=298, Percent_Identity=25.1677852348993, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71980746, Length=262, Percent_Identity=26.3358778625954, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24586117, Length=271, Percent_Identity=27.3062730627306, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221330018, Length=271, Percent_Identity=27.3062730627306, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648690, Length=287, Percent_Identity=26.1324041811847, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015992
- InterPro:   IPR001130
- InterPro:   IPR018228
- InterPro:   IPR012278
- InterPro:   IPR015991 [H]

Pfam domain/function: PF01026 TatD_DNase [H]

EC number: 3.1.21.-

Molecular weight: Translated: 30173; Mature: 30173

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS01137 TATD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVDSHCHIDFPELAERLPQLLENMRTNQVSHALCISVTLEDFPRVLALAEQHPNLYASV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCCEEEEC
GVHPDYEEGAEEPTLERLVTLSAHPRVVGTGETGLDYYRLNGRSIAEMEWQRERFRTHIR
CCCCCHHHCCCCHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
AARQTGKPLIIHTRSSADDTLRLMREENAGEVGGVMHCFTETWDIARQALDEGFHISFSG
HHHHCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
IVTFKSAADLQETAKKVPLDRMLIETDSPYLAPVPYRGKTNEPAWVRHVGEFIAKLRDVP
EEEECCHHHHHHHHHHCCHHHEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHCCC
VETIAEQTTDNFFRLFKHIDRNSHA
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFVDSHCHIDFPELAERLPQLLENMRTNQVSHALCISVTLEDFPRVLALAEQHPNLYASV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHCCCEEEEC
GVHPDYEEGAEEPTLERLVTLSAHPRVVGTGETGLDYYRLNGRSIAEMEWQRERFRTHIR
CCCCCHHHCCCCHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
AARQTGKPLIIHTRSSADDTLRLMREENAGEVGGVMHCFTETWDIARQALDEGFHISFSG
HHHHCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
IVTFKSAADLQETAKKVPLDRMLIETDSPYLAPVPYRGKTNEPAWVRHVGEFIAKLRDVP
EEEECCHHHHHHHHHHCCHHHEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHCCC
VETIAEQTTDNFFRLFKHIDRNSHA
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]