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The map label for this gene is bigR [H]

Identifier: 93006892

GI number: 93006892

Start: 2535789

End: 2536079

Strand: Direct

Name: bigR [H]

Synonym: Pcryo_2068

Alternate gene names: 93006892

Gene position: 2535789-2536079 (Clockwise)

Preceding gene: 93006889

Following gene: 93006893

Centisome position: 82.87

GC content: 48.8

Gene sequence:

>291_bases
ATGCAGCAAGCATCAATGCAAGCCAGCCAACTGCTTAAGTCACTCTCGCATCCTGATCGCTTGCTCCTCCTCTGTCAACT
AACTCAAGGAGAGTACTGCGTCAGTGAGCTTGAAAGCTTAGTCGGTGTTGGGCAGCCAAGCTTATCGCAGCAGCTTGGTA
TTTTGCGTAAAGATGAGCTAGTGACGACACGCCGTGAAGGCAAGCAGATTTACTATAGTATCGCTAGTGATGATGCATTA
GCCATACTAGAGCTGTTGTATCAGCGTTTTTGTGCCCCTGCTGAGGCATAG

Upstream 100 bases:

>100_bases
TAAGGAGCTCGCTCTGATCACTTCTACTTCAGTACTGAGCACATTTGATACCAATGGTGACAGCAATGACGGCGTTGCGC
CAAAAGACCTTGCTGAAAAA

Downstream 100 bases:

>100_bases
ATGATTGGCTAAATCTCATTGATTAAATTCTGATGTTCATGAACATTTTTTATTGTGAGGACGTACTGCCATGCCCTCTA
TTGCCACTCGACTGATACCT

Product: ArsR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 96; Mature: 96

Protein sequence:

>96_residues
MQQASMQASQLLKSLSHPDRLLLLCQLTQGEYCVSELESLVGVGQPSLSQQLGILRKDELVTTRREGKQIYYSIASDDAL
AILELLYQRFCAPAEA

Sequences:

>Translated_96_residues
MQQASMQASQLLKSLSHPDRLLLLCQLTQGEYCVSELESLVGVGQPSLSQQLGILRKDELVTTRREGKQIYYSIASDDAL
AILELLYQRFCAPAEA
>Mature_96_residues
MQQASMQASQLLKSLSHPDRLLLLCQLTQGEYCVSELESLVGVGQPSLSQQLGILRKDELVTTRREGKQIYYSIASDDAL
AILELLYQRFCAPAEA

Specific function: Represses an operon that comprises at least itself and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=92, Percent_Identity=34.7826086956522, Blast_Score=62, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 10671; Mature: 10671

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQASMQASQLLKSLSHPDRLLLLCQLTQGEYCVSELESLVGVGQPSLSQQLGILRKDEL
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHH
VTTRREGKQIYYSIASDDALAILELLYQRFCAPAEA
HHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQQASMQASQLLKSLSHPDRLLLLCQLTQGEYCVSELESLVGVGQPSLSQQLGILRKDEL
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHH
VTTRREGKQIYYSIASDDALAILELLYQRFCAPAEA
HHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]