| Definition | Psychrobacter cryohalolentis K5 chromosome, complete genome. |
|---|---|
| Accession | NC_007969 |
| Length | 3,059,876 |
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The map label for this gene is priA [H]
Identifier: 93006831
GI number: 93006831
Start: 2467655
End: 2470036
Strand: Direct
Name: priA [H]
Synonym: Pcryo_2007
Alternate gene names: 93006831
Gene position: 2467655-2470036 (Clockwise)
Preceding gene: 93006830
Following gene: 93006832
Centisome position: 80.65
GC content: 45.68
Gene sequence:
>2382_bases ATGCTAAGCTTATTCGTCTCTCCCTCTTTATTTTTATTTCATATTGACTTAATCAAACTGCGAAGCCTTTTTATGTTGGT ACAAGTTGCTCTGCCCGTGCCCCTTTATCGGGTATTTGACTATAGCCTACCGTCCGATATAAGTGCTTTGCCAAATGCTT CGCCAAACAATAGCTCAATACAACTGCCGCAAATTGGCAGTCGTGTTGAGGTGTCCTTTGGTCGTCAGACTTTAATCGGA ATCGTGGTTGCTCATATTGCGCAAGCGGATACCAGCGTACCACTCAATAAGCTGAAACCCATTAATAAGTGTTTAGATGC TGAGTCTATTTTAGATAACAGCTTGCTAAAGCTCGCCTATTGGCTTGCTCGTTATTATCATTATCCGCTTGGTGAGGTGT TAGCGGTCATCTTGCCCACCTTGGTTCGTCAAGGTAAACCGCTAGATTTACTCATTACCCATTGGCGAATTCTGCCACAT ATCACTGATGACGACTTTCGTACTAACGCCAAAAAGCAAAAGCAGCAGTTTGATATGCTAAAGCTACATGGTCAACATGG TGCAAGTGAAGATGTACTATTATTAGAAGGTATGGAGCGTGCTTTTTTAAAAACACTAGAAGATAAGGGTTTGATTGAGC GCTATATTCAACCAAAACAAGCACCTACCTCTGTCAAATTGGCAAAAATGCCCCTTGATCTCAATGAGGAGCAACAGCTT GCCGTTGCAGCGATTGTTGCTGCACATGAATCCAAATGCTATACGGGCTTTTTATTAAATGGCATTACCGGCAGTGGCAA GACTGAAGTCTATCTACAAGCCATGCAAGCAGTATTGGAGGCTGGCAAGCAAGTGCTTATTTTAGTGCCTGAGATTGGGT TAACGCCACAGACGCGTGCGCGTTTTGCCAGTCGCTTCGCTGCGCATATCGTCTTGCTACATTCTGGTATGAACAATACT CATCGATTGCAAGGTTGGCAAGATTGCCGTACGGGTCACGCACAAATCATTATCGGTACGCGTTCGGCAGTACTATATCC GTTTGCAGATTTAGGCTTAATTGTCGTTGATGAAGCGCATGATGGCTCCTATAAACAGCAGGATACCTTGCGCTATCATG CTGCTGATGTCGCACTTTATCGAGGATTCCAAGCCAAAATACCGGTGGTCCTTGGTACGGCTACCCCTTCTCTTGAACAT TTAAAATTAGTCGATGAGGGCAAGCTAGTAGAGTGTCAATTATCGACGCGCCCTGGCAATGCTAAACCTGCGCCCATGCA GCTGATTGATGCGCGCTTGCAAAGTACTCATCAGCAAACGCAAGATGATGGCGCGCGCTATGATACCGGACTGACGGATG CACTTATCGGCGCCATAAGGCAAACCCTAGAAGCTGGCGATCAAGTTTTGATATTTTTAAACCGTCGCGGCTATGCGCCT GTTTTATTATGCGAGGCGTGTGGTTGGCAGGCAGATTGTCCGCGCTGCGACGCGCATTTAACCGTTCATTATAATAATCA AGCCCAGCGTAGCAATTATTCAAGCAACTCTTATTTAAAATGCCATCATTGTGATTGGCAAGCCTATATTCCGACTGTAT GCCCTGATTGTGGCAGTCAAAATCTAGATGCCAAAGGGATGGGAACAACAAGGCTAAGCGAAAATTTGCACGCTATCTTT GCCAATCCGCAAACCAGTAAAGCGCTTTATCCCATTATTCAAATTGATCGTGATACCACTAGGCGTAAAGACAGCTGGGA AAATATCTATCAGCGTATTAATGCAGGAAATCCTGCCATATTGGTCGGTACACAAATGGTTGCCAAAGGTCATCATTTTC CCAATGTCACGCTCGTTTGCTTACCCAACGCTGATCGTGGCTTTTTGTCTGCAGACTTTCGCTCGCCAGAGCATACTGCC CAATTGATGATCCAAGTAGCGGGGCGTGCAGGACGCGGGGATAAATCAGGACGGGTATTGATTCAAACGCTGCAACCGGA CAATGCGTTATTATTAAAATTGGTGAAAGACGGTTATTTGTCTTTTGCTCGTGAGCTGTTGCAAGAGCGTAAAATGATGG GACTGCCCCCTCATAGTCATGCTGCGCTGATACGCTGCGAAGGTAAAACTCTTGCTGCCACCACACAAGCACTCAAAGAT GCCATTGCCGTTCTGCCAACAGGGCACAATTTAGCCGTTCTTGGTCCTATCGATGCGCCCATGAGTAAAAAGAACAGTCG CTATCATGCCCAGCTTTTGCTATTGGCCAAGGATCGTCAGCAACTGCATCATGTTTTAAATCATTGGTGGCAACCTGTCC TTACCCTACCGAGCGCTAAATATCTTAAGCTTACTTTAGATATTGACCCTGTCGGCTGGTAA
Upstream 100 bases:
>100_bases CGTGGTCGAAATTGAGTCTATTCTATATATTGAATAAGTTAGATATTCAATACTGCTTGAATAACAATATTTCATATTAG ATCACCATAAGCTTAGCCTC
Downstream 100 bases:
>100_bases CGCAAATTTGCGAGTTATTGCTAGCATAATCATTTGTCGCATTACTCTTTTATCTTTACTTCGTCAGTACAGTTTCTAAT ATTTGACTATGATAAATTTA
Product: primosomal protein N'
Products: NA
Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]
Number of amino acids: Translated: 793; Mature: 793
Protein sequence:
>793_residues MLSLFVSPSLFLFHIDLIKLRSLFMLVQVALPVPLYRVFDYSLPSDISALPNASPNNSSIQLPQIGSRVEVSFGRQTLIG IVVAHIAQADTSVPLNKLKPINKCLDAESILDNSLLKLAYWLARYYHYPLGEVLAVILPTLVRQGKPLDLLITHWRILPH ITDDDFRTNAKKQKQQFDMLKLHGQHGASEDVLLLEGMERAFLKTLEDKGLIERYIQPKQAPTSVKLAKMPLDLNEEQQL AVAAIVAAHESKCYTGFLLNGITGSGKTEVYLQAMQAVLEAGKQVLILVPEIGLTPQTRARFASRFAAHIVLLHSGMNNT HRLQGWQDCRTGHAQIIIGTRSAVLYPFADLGLIVVDEAHDGSYKQQDTLRYHAADVALYRGFQAKIPVVLGTATPSLEH LKLVDEGKLVECQLSTRPGNAKPAPMQLIDARLQSTHQQTQDDGARYDTGLTDALIGAIRQTLEAGDQVLIFLNRRGYAP VLLCEACGWQADCPRCDAHLTVHYNNQAQRSNYSSNSYLKCHHCDWQAYIPTVCPDCGSQNLDAKGMGTTRLSENLHAIF ANPQTSKALYPIIQIDRDTTRRKDSWENIYQRINAGNPAILVGTQMVAKGHHFPNVTLVCLPNADRGFLSADFRSPEHTA QLMIQVAGRAGRGDKSGRVLIQTLQPDNALLLKLVKDGYLSFARELLQERKMMGLPPHSHAALIRCEGKTLAATTQALKD AIAVLPTGHNLAVLGPIDAPMSKKNSRYHAQLLLLAKDRQQLHHVLNHWWQPVLTLPSAKYLKLTLDIDPVGW
Sequences:
>Translated_793_residues MLSLFVSPSLFLFHIDLIKLRSLFMLVQVALPVPLYRVFDYSLPSDISALPNASPNNSSIQLPQIGSRVEVSFGRQTLIG IVVAHIAQADTSVPLNKLKPINKCLDAESILDNSLLKLAYWLARYYHYPLGEVLAVILPTLVRQGKPLDLLITHWRILPH ITDDDFRTNAKKQKQQFDMLKLHGQHGASEDVLLLEGMERAFLKTLEDKGLIERYIQPKQAPTSVKLAKMPLDLNEEQQL AVAAIVAAHESKCYTGFLLNGITGSGKTEVYLQAMQAVLEAGKQVLILVPEIGLTPQTRARFASRFAAHIVLLHSGMNNT HRLQGWQDCRTGHAQIIIGTRSAVLYPFADLGLIVVDEAHDGSYKQQDTLRYHAADVALYRGFQAKIPVVLGTATPSLEH LKLVDEGKLVECQLSTRPGNAKPAPMQLIDARLQSTHQQTQDDGARYDTGLTDALIGAIRQTLEAGDQVLIFLNRRGYAP VLLCEACGWQADCPRCDAHLTVHYNNQAQRSNYSSNSYLKCHHCDWQAYIPTVCPDCGSQNLDAKGMGTTRLSENLHAIF ANPQTSKALYPIIQIDRDTTRRKDSWENIYQRINAGNPAILVGTQMVAKGHHFPNVTLVCLPNADRGFLSADFRSPEHTA QLMIQVAGRAGRGDKSGRVLIQTLQPDNALLLKLVKDGYLSFARELLQERKMMGLPPHSHAALIRCEGKTLAATTQALKD AIAVLPTGHNLAVLGPIDAPMSKKNSRYHAQLLLLAKDRQQLHHVLNHWWQPVLTLPSAKYLKLTLDIDPVGW >Mature_793_residues MLSLFVSPSLFLFHIDLIKLRSLFMLVQVALPVPLYRVFDYSLPSDISALPNASPNNSSIQLPQIGSRVEVSFGRQTLIG IVVAHIAQADTSVPLNKLKPINKCLDAESILDNSLLKLAYWLARYYHYPLGEVLAVILPTLVRQGKPLDLLITHWRILPH ITDDDFRTNAKKQKQQFDMLKLHGQHGASEDVLLLEGMERAFLKTLEDKGLIERYIQPKQAPTSVKLAKMPLDLNEEQQL AVAAIVAAHESKCYTGFLLNGITGSGKTEVYLQAMQAVLEAGKQVLILVPEIGLTPQTRARFASRFAAHIVLLHSGMNNT HRLQGWQDCRTGHAQIIIGTRSAVLYPFADLGLIVVDEAHDGSYKQQDTLRYHAADVALYRGFQAKIPVVLGTATPSLEH LKLVDEGKLVECQLSTRPGNAKPAPMQLIDARLQSTHQQTQDDGARYDTGLTDALIGAIRQTLEAGDQVLIFLNRRGYAP VLLCEACGWQADCPRCDAHLTVHYNNQAQRSNYSSNSYLKCHHCDWQAYIPTVCPDCGSQNLDAKGMGTTRLSENLHAIF ANPQTSKALYPIIQIDRDTTRRKDSWENIYQRINAGNPAILVGTQMVAKGHHFPNVTLVCLPNADRGFLSADFRSPEHTA QLMIQVAGRAGRGDKSGRVLIQTLQPDNALLLKLVKDGYLSFARELLQERKMMGLPPHSHAALIRCEGKTLAATTQALKD AIAVLPTGHNLAVLGPIDAPMSKKNSRYHAQLLLLAKDRQQLHHVLNHWWQPVLTLPSAKYLKLTLDIDPVGW
Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase
COG id: COG1198
COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 helicase C-terminal domain [H]
Homologues:
Organism=Escherichia coli, GI1790370, Length=775, Percent_Identity=42.3225806451613, Blast_Score=565, Evalue=1e-162,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014001 - InterPro: IPR011545 - InterPro: IPR001650 - InterPro: IPR014021 - InterPro: IPR005259 [H]
Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]
EC number: NA
Molecular weight: Translated: 88005; Mature: 88005
Theoretical pI: Translated: 8.60; Mature: 8.60
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.8 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 1.8 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLSLFVSPSLFLFHIDLIKLRSLFMLVQVALPVPLYRVFDYSLPSDISALPNASPNNSSI CEEEEECCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHCCCCCCCCCEE QLPQIGSRVEVSFGRQTLIGIVVAHIAQADTSVPLNKLKPINKCLDAESILDNSLLKLAY ECCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHCHHHHHHHHHHHHHH WLARYYHYPLGEVLAVILPTLVRQGKPLDLLITHWRILPHITDDDFRTNAKKQKQQFDML HHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCHHCCHHHHHHHHHHH KLHGQHGASEDVLLLEGMERAFLKTLEDKGLIERYIQPKQAPTSVKLAKMPLDLNEEQQL HHCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCCCCHHH AVAAIVAAHESKCYTGFLLNGITGSGKTEVYLQAMQAVLEAGKQVLILVPEIGLTPQTRA HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHH RFASRFAAHIVLLHSGMNNTHRLQGWQDCRTGHAQIIIGTRSAVLYPFADLGLIVVDEAH HHHHHHHHHEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCCEEEEECCCCCEEEEECCC DGSYKQQDTLRYHAADVALYRGFQAKIPVVLGTATPSLEHLKLVDEGKLVECQLSTRPGN CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHEEEECCCCEEEEEEECCCCC AKPAPMQLIDARLQSTHQQTQDDGARYDTGLTDALIGAIRQTLEAGDQVLIFLNRRGYAP CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCC VLLCEACGWQADCPRCDAHLTVHYNNQAQRSNYSSNSYLKCHHCDWQAYIPTVCPDCGSQ EEEEECCCCCCCCCCCCCEEEEEECCCHHHCCCCCCCEEEEEECCCCEECCCCCCCCCCC NLDAKGMGTTRLSENLHAIFANPQTSKALYPIIQIDRDTTRRKDSWENIYQRINAGNPAI CCCCCCCCCHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEE LVGTQMVAKGHHFPNVTLVCLPNADRGFLSADFRSPEHTAQLMIQVAGRAGRGDKSGRVL EEECHHHHCCCCCCCEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEE IQTLQPDNALLLKLVKDGYLSFARELLQERKMMGLPPHSHAALIRCEGKTLAATTQALKD EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHH AIAVLPTGHNLAVLGPIDAPMSKKNSRYHAQLLLLAKDRQQLHHVLNHWWQPVLTLPSAK HHHEECCCCCEEEEECCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCHHEECCCCE YLKLTLDIDPVGW EEEEEEECCCCCC >Mature Secondary Structure MLSLFVSPSLFLFHIDLIKLRSLFMLVQVALPVPLYRVFDYSLPSDISALPNASPNNSSI CEEEEECCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHCCCCCCCCCEE QLPQIGSRVEVSFGRQTLIGIVVAHIAQADTSVPLNKLKPINKCLDAESILDNSLLKLAY ECCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHCHHHHHHHHHHHHHH WLARYYHYPLGEVLAVILPTLVRQGKPLDLLITHWRILPHITDDDFRTNAKKQKQQFDML HHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCHHCCHHHHHHHHHHH KLHGQHGASEDVLLLEGMERAFLKTLEDKGLIERYIQPKQAPTSVKLAKMPLDLNEEQQL HHCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCCCCHHH AVAAIVAAHESKCYTGFLLNGITGSGKTEVYLQAMQAVLEAGKQVLILVPEIGLTPQTRA HHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHH RFASRFAAHIVLLHSGMNNTHRLQGWQDCRTGHAQIIIGTRSAVLYPFADLGLIVVDEAH HHHHHHHHHEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCCEEEEECCCCCEEEEECCC DGSYKQQDTLRYHAADVALYRGFQAKIPVVLGTATPSLEHLKLVDEGKLVECQLSTRPGN CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHEEEECCCCEEEEEEECCCCC AKPAPMQLIDARLQSTHQQTQDDGARYDTGLTDALIGAIRQTLEAGDQVLIFLNRRGYAP CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCC VLLCEACGWQADCPRCDAHLTVHYNNQAQRSNYSSNSYLKCHHCDWQAYIPTVCPDCGSQ EEEEECCCCCCCCCCCCCEEEEEECCCHHHCCCCCCCEEEEEECCCCEECCCCCCCCCCC NLDAKGMGTTRLSENLHAIFANPQTSKALYPIIQIDRDTTRRKDSWENIYQRINAGNPAI CCCCCCCCCHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEE LVGTQMVAKGHHFPNVTLVCLPNADRGFLSADFRSPEHTAQLMIQVAGRAGRGDKSGRVL EEECHHHHCCCCCCCEEEEEECCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEE IQTLQPDNALLLKLVKDGYLSFARELLQERKMMGLPPHSHAALIRCEGKTLAATTQALKD EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHH AIAVLPTGHNLAVLGPIDAPMSKKNSRYHAQLLLLAKDRQQLHHVLNHWWQPVLTLPSAK HHHEECCCCCEEEEECCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCHHEECCCCE YLKLTLDIDPVGW EEEEEEECCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]