Click here to switch to the map view.

The map label for this gene is mnmC

Identifier: 93006801

GI number: 93006801

Start: 2436644

End: 2438737

Strand: Direct

Name: mnmC

Synonym: Pcryo_1977

Alternate gene names: 93006801

Gene position: 2436644-2438737 (Clockwise)

Preceding gene: 93006800

Following gene: 93006802

Centisome position: 79.63

GC content: 44.32

Gene sequence:

>2094_bases
ATGCCCAAACCAGCTTCTATGGCTATGAATACAACCAAAAGCACGACACCGGCACATGTTATCTCTCCAGCCAACATTGA
GTGGCAAACTGATGACGCTGGTAATGAAGTGCCAGTATCAGGTGAGTTTGGCGATGCCTATTTTTCGCAAGCAGATGGTT
TAGCAGAATCTCACCATGTATTTTTGGCACATAATCAGCTGCCCACGCGATTGGCAAACCTTATTCCTAAGCAATGCTTT
ACGATCTACGAACTAGGTTTTGGTACAGGACTTAATTTACTCGCGACTTGGCAGTTGTGGCGACAGCTTCGATTAACACA
TCCGCACTTAGCAAGCGCCCGTTTGCACTTTATTACTACCGAAAAACACCCCGTCCCCTTTAGTGACTTAGCAAAGATAC
TTGCCCCACTGGGACAACGTACACCTGAACTGGCACTGTTGATTGAGCAACTTTTAACCAGTTATCCTCCTCTTATCGCT
GGTTGTCATCGGCTAGATTTTATTGATGATAATTTAACCCTTGATATTTGGCTAGGTGATGCTAATGATAGCTTAGCAAG
TTTAGATAGTACAGTAGCTACTCAGCGTCCGTACATCAATGCCTGGTTTCTAGATGGGTTTGCGCCTTCTTGCAATGAGA
TCTTATGGACAGAGCGTATCTTTAGCCACATGCAACGCCTATCACGTGCTGGCACGACTGCTGCTACCTATAGCTGTGCA
GGTATCATCAAACGTGGATTACAGTCGCAGGGTTTTGTCATTAAAAAGAGCAAAGTGCTTGGGTCTAAGCGTGAGATGCT
CACGGCCGTTATGAGCGAGATGTCATCTTTAGATAAGCCTATTTCAACCTTATCTAATAAGGTGACTTTGTCCAACTATC
CTAATAATGCAGTCGTTATCGGTGCTGGTGTGGCAGGACTACTGACTGCATGGTCACTTGCCAATCGTGGTATAAGCGTG
ACAGTGCTCGATAAAGACGCACCGCTGGCTGGTGCATCCGGTAATCCGCGTGCCCTACTCGCCCCGAATATGACGCCTAT
TCATCATGTTTATGAGCATCTGCATTCTATTGGTTATTTATACAGTGGTCGATTATATCGATACTTTAATCAGCAAGCTG
CTATCCAAAAATCGCCGCTGATACTTGAGCAGACCGGCACATTAGACTTATTGGTAAAAACTAATATTGGTACGGAGCAG
ATTTTAGATTATCCAGATACTATGGCAACCACTATTAGCCCCGAAAAAGCACAGGATATCAGTGGTTTAAAAGATAAAGA
CTTAGCTCATAATTTGTATATGCCGCAGGCTGGTTTGGTCAATCCACAAGCCTTACAAACTGTTATTCTTGCGCACCCCA
ATATCACCTATCAGCAGCTCAATATTATTAATATTAAGGAAACCGAGAACAGTATCATTTTGACAGGTTCTAAAGAGGAT
GAAAATGCGAGCCAAGCCTCTTTGACTATCACAGCTGACCATGTTGTGATTTGCGCGGCTTATGAAAGCCATCAATTGGA
TAAACGTATCGTTAAGTGCCGGACGATTCGCGGGCAACTGTCTTGGTTTACACCAACTGCGCAGCAACTGACTCAATTGC
CTAAATTGCCGCTCAAATACAGTGGCTACTGTGCGCCATTTATTGGACAGAGCGGTGATGCGGAACTGAACCATATTAGC
GAAAATCAGCCACAGTTTTTACTTGGTGCAAGCTTTATCGATGGCGATACCAATATCGATGTCCGAGATGAAGAAAATCA
GCAAAATTATGACAAGTTGATTGAAGATATGCCTGAATTAAGCAGCGTCTTGCCAAATGATATCAGTACATGGCATGCGC
GTGCTGGCATTCGTACTCAAACGATGGATTATCATCCTTTGGTCGGATTACTTGCACAGAGTCAGCGTTTATGGAGCATG
TCAGCAATGGGTGCAAAAGGTTATGCGATTGCACCTATATGTGCCGAAGCCTTAGCAGATATGATGTTAGGCTGTTTTAC
ACCTTTATCTGCCGCTATGTTGGCGCGCCTATCGCCCAATCGGGCGCGGCTACATAAAGTACATAAGCATAAAACACGTC
AAGCAGTAATTTAG

Upstream 100 bases:

>100_bases
GTTGCATTTTTATCGATTGAAATCATCTATTGGATTGATGTCTACTGATTTGATGCTCCACTTCCTACTTGCTTGACCAT
TGTGGGGTTGATAAATAATT

Downstream 100 bases:

>100_bases
GTTCAGTACCTTAGCTATATGCCTAATTATGACCCTCACTAATGGATAGCTGTCTTATTATGAAAACTCTTCCCTCTACC
TTTACCTTTACCTTTATAAA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MPKPASMAMNTTKSTTPAHVISPANIEWQTDDAGNEVPVSGEFGDAYFSQADGLAESHHVFLAHNQLPTRLANLIPKQCF
TIYELGFGTGLNLLATWQLWRQLRLTHPHLASARLHFITTEKHPVPFSDLAKILAPLGQRTPELALLIEQLLTSYPPLIA
GCHRLDFIDDNLTLDIWLGDANDSLASLDSTVATQRPYINAWFLDGFAPSCNEILWTERIFSHMQRLSRAGTTAATYSCA
GIIKRGLQSQGFVIKKSKVLGSKREMLTAVMSEMSSLDKPISTLSNKVTLSNYPNNAVVIGAGVAGLLTAWSLANRGISV
TVLDKDAPLAGASGNPRALLAPNMTPIHHVYEHLHSIGYLYSGRLYRYFNQQAAIQKSPLILEQTGTLDLLVKTNIGTEQ
ILDYPDTMATTISPEKAQDISGLKDKDLAHNLYMPQAGLVNPQALQTVILAHPNITYQQLNIINIKETENSIILTGSKED
ENASQASLTITADHVVICAAYESHQLDKRIVKCRTIRGQLSWFTPTAQQLTQLPKLPLKYSGYCAPFIGQSGDAELNHIS
ENQPQFLLGASFIDGDTNIDVRDEENQQNYDKLIEDMPELSSVLPNDISTWHARAGIRTQTMDYHPLVGLLAQSQRLWSM
SAMGAKGYAIAPICAEALADMMLGCFTPLSAAMLARLSPNRARLHKVHKHKTRQAVI

Sequences:

>Translated_697_residues
MPKPASMAMNTTKSTTPAHVISPANIEWQTDDAGNEVPVSGEFGDAYFSQADGLAESHHVFLAHNQLPTRLANLIPKQCF
TIYELGFGTGLNLLATWQLWRQLRLTHPHLASARLHFITTEKHPVPFSDLAKILAPLGQRTPELALLIEQLLTSYPPLIA
GCHRLDFIDDNLTLDIWLGDANDSLASLDSTVATQRPYINAWFLDGFAPSCNEILWTERIFSHMQRLSRAGTTAATYSCA
GIIKRGLQSQGFVIKKSKVLGSKREMLTAVMSEMSSLDKPISTLSNKVTLSNYPNNAVVIGAGVAGLLTAWSLANRGISV
TVLDKDAPLAGASGNPRALLAPNMTPIHHVYEHLHSIGYLYSGRLYRYFNQQAAIQKSPLILEQTGTLDLLVKTNIGTEQ
ILDYPDTMATTISPEKAQDISGLKDKDLAHNLYMPQAGLVNPQALQTVILAHPNITYQQLNIINIKETENSIILTGSKED
ENASQASLTITADHVVICAAYESHQLDKRIVKCRTIRGQLSWFTPTAQQLTQLPKLPLKYSGYCAPFIGQSGDAELNHIS
ENQPQFLLGASFIDGDTNIDVRDEENQQNYDKLIEDMPELSSVLPNDISTWHARAGIRTQTMDYHPLVGLLAQSQRLWSM
SAMGAKGYAIAPICAEALADMMLGCFTPLSAAMLARLSPNRARLHKVHKHKTRQAVI
>Mature_696_residues
PKPASMAMNTTKSTTPAHVISPANIEWQTDDAGNEVPVSGEFGDAYFSQADGLAESHHVFLAHNQLPTRLANLIPKQCFT
IYELGFGTGLNLLATWQLWRQLRLTHPHLASARLHFITTEKHPVPFSDLAKILAPLGQRTPELALLIEQLLTSYPPLIAG
CHRLDFIDDNLTLDIWLGDANDSLASLDSTVATQRPYINAWFLDGFAPSCNEILWTERIFSHMQRLSRAGTTAATYSCAG
IIKRGLQSQGFVIKKSKVLGSKREMLTAVMSEMSSLDKPISTLSNKVTLSNYPNNAVVIGAGVAGLLTAWSLANRGISVT
VLDKDAPLAGASGNPRALLAPNMTPIHHVYEHLHSIGYLYSGRLYRYFNQQAAIQKSPLILEQTGTLDLLVKTNIGTEQI
LDYPDTMATTISPEKAQDISGLKDKDLAHNLYMPQAGLVNPQALQTVILAHPNITYQQLNIINIKETENSIILTGSKEDE
NASQASLTITADHVVICAAYESHQLDKRIVKCRTIRGQLSWFTPTAQQLTQLPKLPLKYSGYCAPFIGQSGDAELNHISE
NQPQFLLGASFIDGDTNIDVRDEENQQNYDKLIEDMPELSSVLPNDISTWHARAGIRTQTMDYHPLVGLLAQSQRLWSMS
AMGAKGYAIAPICAEALADMMLGCFTPLSAAMLARLSPNRARLHKVHKHKTRQAVI

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=705, Percent_Identity=32.3404255319149, Blast_Score=306, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_PSYCK (Q1Q999)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_581238.1
- ProteinModelPortal:   Q1Q999
- SMR:   Q1Q999
- STRING:   Q1Q999
- GeneID:   4035074
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_1977
- NMPDR:   fig|335284.3.peg.1572
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   WLILPHL
- PhylomeDB:   Q1Q999
- ProtClustDB:   CLSK830736
- BioCyc:   PCRY335284:PCRYO_1977-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 76653; Mature: 76521

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKPASMAMNTTKSTTPAHVISPANIEWQTDDAGNEVPVSGEFGDAYFSQADGLAESHHV
CCCCCCCEECCCCCCCCCEEECCCCCEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCEE
FLAHNQLPTRLANLIPKQCFTIYELGFGTGLNLLATWQLWRQLRLTHPHLASARLHFITT
EEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
EKHPVPFSDLAKILAPLGQRTPELALLIEQLLTSYPPLIAGCHRLDFIDDNLTLDIWLGD
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHCCHHCEEECCCEEEEEEECC
ANDSLASLDSTVATQRPYINAWFLDGFAPSCNEILWTERIFSHMQRLSRAGTTAATYSCA
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GIIKRGLQSQGFVIKKSKVLGSKREMLTAVMSEMSSLDKPISTLSNKVTLSNYPNNAVVI
HHHHHCCCCCCEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
GAGVAGLLTAWSLANRGISVTVLDKDAPLAGASGNPRALLAPNMTPIHHVYEHLHSIGYL
ECCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHH
YSGRLYRYFNQQAAIQKSPLILEQTGTLDLLVKTNIGTEQILDYPDTMATTISPEKAQDI
HCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCHHHHHCCCCHHEECCCCHHHHHH
SGLKDKDLAHNLYMPQAGLVNPQALQTVILAHPNITYQQLNIINIKETENSIILTGSKED
CCCCCCHHHHHCCCCCCCCCCHHHHHEEEEECCCCEEEEEEEEEEEECCCCEEEECCCCC
ENASQASLTITADHVVICAAYESHQLDKRIVKCRTIRGQLSWFTPTAQQLTQLPKLPLKY
CCCCCEEEEEEECCEEEEEECHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHCCCCCCCC
SGYCAPFIGQSGDAELNHISENQPQFLLGASFIDGDTNIDVRDEENQQNYDKLIEDMPEL
CCEECCCCCCCCCCCHHCCCCCCCCEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHH
SSVLPNDISTWHARAGIRTQTMDYHPLVGLLAQSQRLWSMSAMGAKGYAIAPICAEALAD
HHHCCCCHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
MMLGCFTPLSAAMLARLSPNRARLHKVHKHKTRQAVI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PKPASMAMNTTKSTTPAHVISPANIEWQTDDAGNEVPVSGEFGDAYFSQADGLAESHHV
CCCCCCEECCCCCCCCCEEECCCCCEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCEE
FLAHNQLPTRLANLIPKQCFTIYELGFGTGLNLLATWQLWRQLRLTHPHLASARLHFITT
EEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
EKHPVPFSDLAKILAPLGQRTPELALLIEQLLTSYPPLIAGCHRLDFIDDNLTLDIWLGD
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHCCHHCEEECCCEEEEEEECC
ANDSLASLDSTVATQRPYINAWFLDGFAPSCNEILWTERIFSHMQRLSRAGTTAATYSCA
CCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GIIKRGLQSQGFVIKKSKVLGSKREMLTAVMSEMSSLDKPISTLSNKVTLSNYPNNAVVI
HHHHHCCCCCCEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
GAGVAGLLTAWSLANRGISVTVLDKDAPLAGASGNPRALLAPNMTPIHHVYEHLHSIGYL
ECCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHH
YSGRLYRYFNQQAAIQKSPLILEQTGTLDLLVKTNIGTEQILDYPDTMATTISPEKAQDI
HCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCHHHHHCCCCHHEECCCCHHHHHH
SGLKDKDLAHNLYMPQAGLVNPQALQTVILAHPNITYQQLNIINIKETENSIILTGSKED
CCCCCCHHHHHCCCCCCCCCCHHHHHEEEEECCCCEEEEEEEEEEEECCCCEEEECCCCC
ENASQASLTITADHVVICAAYESHQLDKRIVKCRTIRGQLSWFTPTAQQLTQLPKLPLKY
CCCCCEEEEEEECCEEEEEECHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHCCCCCCCC
SGYCAPFIGQSGDAELNHISENQPQFLLGASFIDGDTNIDVRDEENQQNYDKLIEDMPEL
CCEECCCCCCCCCCCHHCCCCCCCCEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHH
SSVLPNDISTWHARAGIRTQTMDYHPLVGLLAQSQRLWSMSAMGAKGYAIAPICAEALAD
HHHCCCCHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
MMLGCFTPLSAAMLARLSPNRARLHKVHKHKTRQAVI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA